Protective effect of ivabradine on myocardial injury in rats with coronary heart disease

Abstract

Objective To explore protective effect of ivabradine on myocardial injury in rats with coronary heart disease. Methods Twenty rats were fed with high-fat diet and injected with Pituitrin to simulate coronary heart disease. Successful rats were randomly divided into model group and ivabradine group [10 mg/ (kg·d)]. In addition, rats in sham operation group were fed with normal diet, continuous administration for 12 weeks. Cardiac function, apoptotic index (AI), the level of malondialdehyde (MDA), the activity of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GSH-Px), the level of tumor necrosis factor alpha (TNF-α), interleukin (IL)-1β, IL-6, and the expression of nuclear factor-κ-gene binding κ (NF-κB) signal pathway related protein was detected. Results Compared with the model group, LVSP、+ dp/dtmax、-dp/dtmax was increased (P<0.01), AI [(8.35±0.84)% vs. (36.35±3.68)%], the contents of TNF-α [(4.83±0.48) μg/L vs. (14.57±1.46) μg/L], IL-1β [(74.68±7.49) ng/L vs. (245.32±24.53) ng/L, IL-6 [(97.28±9.73) μg/L vs. (221.43±20.68) μg/L] and MDA [(63.82±6.33) μmol/g vs. (84.36±8.44) μmol/g]was decreased (P<0.01), the activities of SOD [(6.29±0.39) U/g vs. (2.78±0.25) U/g], CAT [(12.56±1.26) U/g vs. (8.31±0.83) U/g] and GSH-Px [(14.47±1.45) U/g vs. (25.34±2.57) U/g] was increased (P<0.01), and the expressions of p-IκBα (0.24±0.02 vs. 0.88±0.09) and p-NF-κB p65 (0.32±0.03 vs. 0.72±0.07) was decreased in dexamethasone group (P<0.01). Conclusion Ivabradine might inhibit myocardial injury in rats with coronary heart disease through antioxidant and anti-inflammatory effects Key words: Ivabradine; Coronary heart disease; Rats; Oxidant; Inflammation