МЕТОД ОПРЕДЕЛЕНИЯ СВЕРХЧУВСТВИТЕЛЬНОГО ОТВЕТА РАСТЕНИЙ КАРТОФЕЛЯ НА КЛАДКИ КОЛОРАДСКОГО ЖУКА ДЛЯ ИСПОЛЬЗОВАНИЯ В СЕЛЕКЦИОННОЙ РАБОТЕ

Abstract

The creation of new varieties of potato revealing innate highly effective mechanisms of protection against Colo-rado potato beetle is an urgent task of breeding, which requires the development of methods that allow standardizing certain plant responses to different aspects of the influence of this phytophage under laboratory conditions. The laying of eggs by insects on the surface of plant laves leads to the development of an intense protective reaction and the production of ovicides, intensive death of tissue, followed by the loss of eggs or their drying. To simulate the laying of eggs on the leaves, we used a washout from the surface of the clutches of the Colorado potato beetle containing a pool of elicitors of plant defense reactions. On cultivars Bashkirsky (high resistance to the Colorado potato beetle) and Udacha (medium-resistant cultivar), drying of leaf areas was observed in response to the treatment of the lower surface of the leaf blade, and on cultivar Rannyaya rosa (unstable cultivar), there was no reaction. The dried spots on the leaf blade were strictly limited to the place where the wash was applied. It was found that the formation of reactive oxygen species in response to the application of the washout occurs only on potato varieties Bashkirsky and Udacha with the development of a hypersensitive response, which corresponded to the drying of leaf blades observed under the field conditions at the sites of clutch attachment. Localization of hydrogen peroxide and superoxide anion occurs in the cells of the vascular bundles and adjacent tissues. On the Rannyaya Rosa cultivar, the application of the washout did not lead to the accumulation of reactive oxygen spe-cies in the leaf tissue and the drying of the leaves.