Zucchini Cultivars Research Articles

Production of fruits with attached flowers in zucchini squash is correlated with the arrest of maturation of female flowers

SummaryResults from two trials carried out under different environmental conditions indicated that the high temperatures reached in greenhouses during the Spring-Summer growing season are the main environmental factor inducing production of zucchini fruits with attached flowers. The fact that the incidence of this characteristic is genotypedependent, with the percentage of fruits with attached flowers varying from 1.4 – 73% among the different cultivars grown during the Spring-Summer season, provides an opportunity for direct counter-selection for this trait in current zucchini squash breeding programmes. High temperatures in the Spring-Summer growing season also induced male-ness in all the cultivars analysed, delaying the production of female flowers, and increasing the number of male flowers on the main stem. Indeed, those flowers that remained attached to harvested zucchini fruits were transformed into bisexual flowers, exhibiting different degrees of stamen development, and were arrested as immature, closed floral buds. A detailed analysis of the maturation and abscission times in female and male flowers of different zucchini cultivars indicated that, although abscission time did not differ, maturation time was longer in male than in female flowers. By comparing sexual expression in different cultivars in different environments, we concluded that inhibition of female flower bud maturation occurs concomitantly with a delay in flower abscission, a process accompanied by the conversion of the female bud into a bisexual bud. Given that Spring-Summer conditions promote the maturation of both female and male flowers, and that the arrest of female flower maturation and abscission are associated with floral sex determination, it is possible that the arrest of female flower maturation is not directly induced by high temperatures, but is mediated instead by hormones such as ethylene or gibberellins.

(257) Evaluation and Selection of Squash Types and Cultivars for Production of Edible Squash Blossoms

Recently, an increasing number of restaurants in Palm Beach County, Florida, have been requesting squash (Cucurbita pepo) flowers from local vegetable growers. Typically, current field-grown squash cultivars produce a higher ratio of female to male flowers, with the emphasis on fruit production. However, a market for squash blossoms indicates a need for cultivars that produce higher numbers of consistently developing male flowers throughout the growing season. In order to evaluate male squash blossom production, 10 squash cultivars, including yellow-summer, zucchini, round, and scallop-types, and one compact-type pumpkin, were field-grown during the 2005–06 growing season. The average number of male flowers per plant by week was recorded for 7 weeks, starting when the first male flowers were identified within the entire trial. In addition to blossom counts, flower traits, such as bell height, depth, volume, and weight were also recorded. Preliminary results from the 2005 season indicate that the commercial yellow-summer squash cultivars, Mulitpik and Early Prolific Straightneck, and the zucchini cultivars, Jaguar and Raven, produced fewer male flowers on a week-by-week and total basis. The cultivar, White Bush Scallop, produced significantly more male flowers then any other entry, with an average of 9.8 male flowers per plant per week. Little or no difference was seen in bell height and depth among the 11 cultivars; however, two cultivars, Costa Romanesque and Hybrid Pam (compact pumpkin type) had significantly greater bell volumes and weights, indicating a much larger blossom size.

Effect of mycorrhizal fungi on the phytoextraction of weathered p, p-DDE by Cucurbita pepo

Field experiments were conducted to assess the impact of inoculation with mycorrhizal fungi on the accumulation of weathered p, p′-DDE from soil by three cultivars of zucchini ( Cucurbita pepo spp. pepo cv Costata Romanesco, Goldrush, Raven). Three commercially available mycorrhizal products (BioVam, Myco-Vam™, INVAM) were inoculated into the root system of the zucchini seedlings at planting. In agreement with our previous findings, plants not inoculated with fungi accumulated large but variable amounts of contaminant, with root bioconcentration factors (BCFs, ratio of p, p′-DDE, on a dry weight basis, in the root to that in the soil) ranging from 10 to 48 and stem BCFs ranging from 5.5 to 11. The total amount of contaminant phytoextracted during the 62 day growing season ranged from 0.72–2.9%. The effect of fungal inoculation on the release of weathered p, p′-DDE from soil and on the subsequent uptake of the parent compound by zucchini appeared to vary at the cultivar level. For Goldrush, fungal inoculation generally decreased tissue BCFs but because of slightly larger biomass, did not significantly impact the percent contaminant phytoextracted. Alternatively, for Costata, BioVam and Myco-Vam™ generally enhanced p, p′-DDE accumulation from soil, and increased the amount of contaminant phytoextracted by up to 34%. For Raven, BioVam reduced contaminant uptake whereas Myco-Vam TM and INVAM increased contaminant phytoextraction by 53 and 60%, respectively. The data show that fungal inoculation may significantly increase the remedial potential of C. pepo ssp. pepo. The apparent cultivar specific response to mycorrhizal inoculation is unexpected and the subject of ongoing investigation.

Analysis of Biogenic Volatile Organic Compounds in Zucchini Flowers: Identification of Scent Sources

An analytical method has been applied to determine volatile organic compounds in zucchini flowers. In a first step, the analytical method was applied to characterize the main scents emitted by whole male and female living flowers of three main commercial cultivars of zucchini (Tosca, Chapin, and Consul). In a second step, the compounds were quantified in different parts of the living flowers to identify the contribution of nectar, petals, anther, and stigma to the aroma profile of the flower. The analytical method is based on headspace solid-phase microextraction coupled on-line with GC and tandem MS detection (HS-SPME-GC-MS/MS). A reference compound is added to samples as part of the field quality control procedure to check for likely analyte losses or sample decomposition. The reference compound also acts as an internal standard for quantification purposes. Results have been statistically studied applying principal component analysis (PCA), which shows that three components explain more than 91% of the variance. PCA emphasizes the great importance of nectar as being the main source of 1,4-dimethoxybenzene and 1,2,4-trimethoxybenzene, which influence the aroma profile of flowers. The remaining components can be accounted for by emissions from petals and sexual organs (adroecium and gynoecium anthers or stigmas).

Increase in Zucchini yellow mosaic virus Symptom Severity in Tolerant Zucchini Cultivars Is Related to a Point Mutation in P3 Protein and Is Associated with a Loss of Relative Fitness on Susceptible Plants

ABSTRACT Zucchini yellow mosaic virus (ZYMV, Potyvirus) is a very damaging cucurbit virus worldwide. Interspecific crosses with resistant Cucurbita moschata have led to the release of "resistant" zucchini squash (C. pepo) F(1) hybrids. However, although the resistance is almost complete in C. moschata, the commercial C. pepo hybrids are only tolerant. ZYMV evolution toward increased aggressiveness on tolerant hybrids was observed in the field and was obtained experimentally. Sequence comparisons and recombination experiments revealed that a point mutation in the P3 protein of ZYMV was enough to induce tolerance breaking. Competition experiments were performed between quasi-isogenic wild-type, and aggressive variants of ZYMV distinguished by monoclonal antibodies. The aggressive mutants were more fit than wild-type strains in mixed infections of tolerant zucchini, but they presented a drastic fitness loss in mixed infections of susceptible zucchini or melon. Thus, the ability to induce severe symptoms in tolerant zucchini is related to a genetic load in susceptible zucchini, but also on other susceptible hosts. This represents the first quantitative study of the fitness cost associated with tolerance breaking for a plant virus. Thus, although easily broken, the tolerance might prove durable in some conditions if the aggressive variants are counterselected in susceptible crops.

Occurrence of races and pathotypes of cucurbit powdery mildew in southeastern Spain

The causal agent of cucurbit powdery mildew in southeastern Spain has been investigated since 1996. Of the 139 single-spore isolates obtained, all were identified asSphaerotheca fusca. Four physiological races (1, 2, 4 and 5) of the pathogen were detected. During the survey, a population shift ofS. fusca was observed: race 1 was progressively replaced by other races. In addition, race coexistence was observed in several cucurbit greenhouses. Four host range patterns or pathotypes were distinguished among the isolates ofS. fusca. All isolates were highly aggressive on melon and especially on zucchini cultivars. No clear relationships between races and pathotypes could be established, although isolates virulent on watermelon were preferentially associated with race 1. These data show an apparent heterogeneity ofS. fusca populations in southeastern Spain that should be analyzed further.

Tolerance of Cucurbits to the Herbicides Clomazone, Ethalfluralin, and Pendimenthalin. I. Summer Squash

Field studies were conducted in 1993, 1994, and 1996 to determine the tolerance of several cultivars of zucchini and yellow crookneck squash (Cucurbita pepo L.) to various rates and methods of application of clomazone, ethalfluralin, and pendimethalin. Applying herbicides preplant soil incorporated (PPI), preemergence (PRE), at seedling emergence (SE), or early postemergence (EPOT) resulted in plant injury that varied from 0% to 98%. Ethalfluralin and pendimethalin (PPI) at 1.12 kg·ha–1 a.i. resulted in the greatest stand and yield reductions across all cultivars. Fruit number and weight declined for all cultivars in 1993 and 1994 as the amount of pendimethalin applied PRE was increased. Zucchini (`Senator') fruit size was significantly reduced for the first three harvests in 1993 by PRE application of pendimethalin or PPI application of ethalfluralin, at all rates. Yellow squash (`Dixie') fruit size was unaffected by herbicide treatment for any harvests during 1993 or 1996. Yellow and zucchini squash yield, fruit number, and average fruit weight were equal to, or greater than, those of the untreated control for PRE clomazone using either the emulsifiable concentrate formulation (EC) during 1993, 1994, and 1996 or the microencapsulated formulation (ME) during 1996. Foliar bleaching and stunting by clomazone was evident in early-season visual observations and ratings, but the effect was transient. Foliar bleaching by clomazone PPI (1.12 kg·ha–1 a.i.) was more evident in `Senator' zucchini, and yield was significantly reduced in 1993. These effects of clomazone PPI were not evident in 1994 for either `Elite' or `Senator' zucchini squash. Chemical names used: 2-[(2-chlorophenyl)methyl]-4, 4-dimethyl-3-isoxazolidinone (clomazone); N-ethyl-N-(2-methyl-2-propenyl)-2,6-dinitro-4-(trifluoromethyl) benzenamine (ethalfluralin); N-(1-ethylopropyl)-3,4-dimethyl-2,6-dinitrobenzenamine (pendimethalin).

Differential susceptibility of commercial zucchini cultivars to viruses of cucurbits

A study to assess the effectiveness of some zucchini [Cucurbita pepo (L.)] cultivars in limiting the effects of virus disease in zucchini crops was conducted at Bayne's Drift, KwaZulu-Natal. The severity of disease was assessed using a visual rating scale. No yield data was collected. ‘SQ 229’, ‘Puma’, ‘SQ 228’ and ‘Gemma’ offered some control. These cultivars may be useful in increasing production in areas severely affected by the virus diseases considered in the study.

Quantitative and Qualitative Changes in Peroxidase of Cucurbita Pepo Cultivars Stressed with Heavy Metals

Seedlings of two cultivars of zucchini (Cucurbita pepo L.) Courgette d'Italie (CI) and Courgette d'Alger (CA) were pre-treated with various concentrations of cadmium, copper and zinc for 30 d. High accumulation of heavy metals especially in the roots was showed. Peroxidase activity was affected according to the type of metal added, concentration, and the plant cultivar used. In leaves and roots of the CI control plants peroxidase activities were 50 and 17 % higher than in the CA control plants. Treatment with Cd (5 µg g−1), Cu (200 µg g−1), and Zn (500 µg g−1) increased peroxidase activities in CA but decreased it in CI both in leaves and roots. Heavy metals tested lead also to some qualitative changes characterized by appearance of new isoforms of peroxidase. The results show the possibility to use the activities of peroxidase as biomarkers for Cd, Cu and Zn stresses.

Resource re‐allocation following fruit removal in cucurbits: patterns in two varieties of squash

summaryThe effects of removal of marketable‐sized fruits on investment toward male, female and vegetative activities were evaluated using two monoecious varieties of cultivated squash, Cucurbita pepo, cv. Striato d'Itaha o di Napoli, and cv. Zucchini Black. Leaf and flower demography were used to examine potential trade‐offs among these activities. Treated individuals produced more leaves, but individual leaf life expectancies were lower than those of controls. Fruit removal also significantly stimulated the production of male and female flowers, fruit and total above‐ground biomass. By contrast, permitting the extended process of fruit maturation in controls significantly decreased the overall production of both male and female flowers. There was no significant difference in total fruit mass between treated and control individuals. However, production of female and male flowers was labile, and responsive to the resource relationships operating within the plant. Treated plants produced more female (potentially fruit‐bearing) flowers as their fruits were harvested, and both groups of plants produced more male flowers at the end of the season. Overall, the squash cultivar produced almost twice as many male flowers as the zucchini cultivar; the harvesting treatment enhanced male flower production later in the season, and did so earlier in squash than in zucchini. Since reduced fruit production is achieved through reduced production of female flowers rather than through abortion of early fruits, and this precedes the period where resource limitation is anticipated, it is concluded that available resources do not directly prevent fruit development. The results are interpreted in the light of recent artificial selection for one to two large fruits per plant in the case of the squash cultivar and many smaller fruits in the case of the zucchini.

Effects of soil fertility and mycorrhizal infection on pollen production and pollen grain size of Cucurbita pepo (Cucurbitaceae)

ABSTRACTThe effects of soil fertility (two levels of soil nitrogen and two levels of soil phosphorus) and mycorrhizal infection on pollen production and pollen grain size were studied in two cultivars of the common zucchini (Cucurbita pepo). Overall, soil fertility and mycorrhizal infection had significant effects on traits affecting the male function of plants (staminate flower production, pollen production per flower and pollen grain size). There were also differences between the cultivars for these male traits in all three experiments. In addition, pollen grain size decreased toward the end of the growing season. In the mycorrhiza experiment, both phosphate concentration per pollen grain and total phosphate content per anther were greater but not significantly greater in the mycorrhizal plants than in the non‐mycorrhizal plants. A significant negative relationship between pollen production and pollen grain size was found in the mycorrhiza and soil phosphorus experiments, indicating that there was a trade‐off between pollen production and pollen size. This study is the first to show that mycorrhizal infection has an effect on male function (pollen production and size) in addition to the well‐documented effects on female function (fruit/seed production and size).

Crop/weed microgametophyte competition inCucurbita pepo (Cucurbitaceae)

Prior studies of crop/weed gene flow in Cucurbita pepo have demonstrated that pollinators can deposit mixed pollen loads. The fate of microgametophytes emerging from these pollen mixtures is unknown. In an effort to define the relationship between pollen mixture composition and progeny composition, experimental plants representing a zucchini cultivar and a cross‐compatible free‐living type were genotyped with an isozyme marker and grown under greenhouse conditions. Weighed mixtures of weed/crop pollen were applied to stigmas of both types in differing ratios. Subsequent fertilizations were tracked by an electrophoretic screen of over 1,600 progeny from 39 fruits. Equal mixtures of weed/crop pollen do not produce a corresponding suite of progeny. Pollen of the pistillate parent is favored, and this advantage increases as the proportion of pollen from the pistillate parent increases. However, increasing the proportion of zucchini pollen does not produce a corresponding increase in fertilization success when the free‐living type is used as pistillate parent. These results indicate that microgametophyte competition in both domesticated and free‐living Cucurbita gynoecia could be a significant component of gene flow dynamics. The nature of this competition can only be defined by experiments that control numerous variables, both paternal and maternal, that might be involved. The tendency for crop/weed hybrids to occur at different portions of the ovary suggests that interactions involving two factors—microgametophyte growth rate and gynoecium structure—might play a significant role.

Crop/Weed Microgametophyte Competition in Cucurbita pepo (Cucurbitaceae)

Prior studies of crop/weed gene flow in Cucurbita pepo have demonstrated that pollinators can deposit mixed pollen loads. The fate of microgametophytes emerging from these pollen mixtures is unknown. In an effort to define the relationship between pollen mixture composition and progeny composition, experimental plants representing a zucchini cultivar and a cross-compatible free-living type were genotyped with an isozyme marker and grown under greenhouse conditions. Weighed mixtures of weed/crop pollen were applied to stigmas of both types in differing ratios. Subsequent fertilizations were tracked by an electrophoretic screen of over 1,600 progeny from 39 fruits. Equal mixtures of weed/crop pollen do not produce a corresponding suite of progeny. Pollen of the pistillate parent is favored, and this advantage increases as the proportion of pollen from the pistillate parent increases. However, increasing the proportion of zucchini pollen does not produce a corresponding increase in fertilization success when the free-living type is used as pistillate parent. These results indicate that microgametophyte competition in both domesticated and free-living Cucurbita gynoecia could be a significant component of gene flow dynamics. The nature of this competition can only be defined by experiments that control numerous variables, both paternal and maternal, that might be involved. The tendency for crop/weed hybrids to occur at different portions of the ovary suggests that interactions involving two factors—microgametophyte growth rate and gynoecium structure—might play a significant role.

Effects of soil phosphorus on pollen production, pollen size, pollen phosphorus content, and the ability to sire seeds in Cucurbita pepo (Cucurbitaceae)

To determine the effects of soil phosphorus on pollen production, pollen grain size, phosphate concentration per pollen grain, and the siring ability of pollen, two cultivars of the common zucchini (Cucurbita pepo) were grown under two soil phosphorus conditions in an experimental garden. Overall, soil phosphorus availability had a significant effect on reproductive output through the female function and on traits affecting the male function of plants (staminate flower production, pollen production per flower, and pollen grain size). In addition, pollen produced by plants in the high phosphorus soils had a higher phosphate concentration than pollen produced by plants in the low phosphorus soils. A pollen mixture experiment revealed that pollen produced by plants in the high phosphorus treatment sired significantly more seeds than pollen produced by plants in the low phosphorus treatment. This study showed that growing conditions such as soil phosphorus can influence the size of a pollen grain and its chemical composition, which, in turn, can affect its ability to sire mature seeds.

151 HIGH YIELDS OF SUMMER SQUASH LINES AND HYBRID COMBINATIONS.

The Gray Zucchini cultivar of summer squash is widely consumed as a fresh market vegetable in Northwest Mexico but is highly suceptible to viral diseases. Gray Zucchini type lines were developed by interspecific hybridization using a local landrace of Cucurbita moschata, which has shown high levels of viral resistance, as male parent and suceptible C. pepo cv. classic as female. The lines were obtained after 4 backcrossing and 3-5 selfing generations. In 1993 average commercial yield of first generation hybrids between lines was 28,155 kg/ha followed by line × Gray Zucchini hybrids, lines, commercial hybrids (Classic. Corsair, Onyx, Raven), and open pollinated cultivars (Gray Zucchini, Black Zucchini) with 26,594, 21,062, 18,862 and 10,172 kg/ha respectively. Yield was inversely related to symptoms of viral infection.

Effects of Soil Nitrogen on Pollen Production, Pollen Grain Size, and Pollen Performance in Cucurbita pepo (Cucurbitaceae)

To determine the effects of soil nitrogen on pollen production, pollen size, and pollen performance, two cultivars of zucchini (Cucurbita pepo) were grown under two nitrogen regimes in an experimental garden. The two cultivars were true breeding for alternative alleles for a one gene trait, ovary color. The soil nitrogen treatment had a significant effect on most measures of reproductive output through the female function. The nitrogen treatment did not affect the number of staminate flowers per plant but did have an effect on the number of pollen grains per staminate flower and the mean pollen grain size. A pollen mixture experiment revealed that pollen produced by plants in the high nitrogen treatment sired significantly more seeds than pollen from low nitrogen plants. Moreover, we found that the high nitrogen pollen sired even a greater percentage of seeds in the region of the fruit (ovary) previously shown to be fertilized by the fastest growing pollen tubes. Thus, the difference in the number of seeds sired by pollen from the two nitrogen treatments is due to differences in pollen performance. We conclude that spatial heterogeneity in soil nitrogen can influence the paternity of seeds in a plant population.

EFFECTS OF SOIL NITROGEN ON POLLEN PRODUCTION, POLLEN GRAIN SIZE, AND POLLEN PERFORMANCE IN CUCURBITA PEPO (CUCURBITACEAE)

To determine the effects of soil nitrogen on pollen production, pollen size, and pollen performance, two cultivars of zucchini (Cucurbita pepo) were grown under two nitrogen regimes in an experimental garden. The two cultivars were true breeding for alternative alleles for a one gene trait, ovary color. The soil nitrogen treatment had a significant effect on most measures of reproductive output through the female function. The nitrogen treatment did not affect the number of staminate flowers per plant but did have an effect on the number of pollen grains per staminate flower and the mean pollen grain size. A pollen mixture experiment revealed that pollen produced by plants in the high nitrogen treatment sired significantly more seeds than pollen from low nitrogen plants. Moreover, we found that the high nitrogen pollen sired even a greater percentage of seeds in the region of the fruit (ovary) previously shown to be fertilized by the fastest growing pollen tubes. Thus, the difference in the number of seeds sired by pollen from the two nitrogen treatments is due to differences in pollen performance. We conclude that spatial heterogeneity in soil nitrogen can influence the paternity of seeds in a plant population.

Effects of genotype on pollen performance in Cucurbita pepo

This study examines the assumption of the pollen competition hypothesis that genetic differences among microgametophytes lead to differences in pollen performance and result in non-random fertilization. In addition, we examined the assumption that pollen performance is genetically correlated with sporophyte vigor due to an overlap in gene expression between the two stages of the life cycle. The results from a pollen mixture experiment in which two cultivars of common zucchini were used show that the ability to sire seeds is nonrandom with respect to the cultivar of the pollen donor plant. The proportion of the progeny sired by the two cultivars is not independent of the region of the fruit where the seeds are produced. The progeny sired by the yellow cultivar outperformed the progeny sired by the green cultivar in a greenhouse study. In addition, the progeny sired by the yellow cultivar from the stylar region of the fruit germinated faster and had more leaf area than the progeny sired by the same cultivar from the peduncular end of the fruit. Thus, the most vigorous progeny are obtained from the stylar region of the fruit where the ovules are fertilized by the most vigorous microgametophytes.

EFFECT OF FRUIT COLOR ON HARVEST SPEED OF ZUCCHINI

Two zucchini (Cucurbita pepo) cultivars having dark green fruits, Ambassador and Bareqet, and one having golden-yellow fruits, Goldy, were compared for speed of harvest under field conditions. Plots of Goldy were harvested 18%, fruits 20%, marketable fruits 26% and Grade A fruits 33% faster than those of Ambassador or Bareqet. All three cultivars had an open growth habit and produced nearly identical numbers of fruits. The results indicate that the differences in harvest speed among the cultivars were based mainly on camouflage of the green fruits and contrast of the golden-yellow fruits with the green foliage.Key words: Zucchini, Cucurbita pepo, gene B, summer squash, marrow, courgette

Yield and Yield Quality in Precocious Yellow Zucchini Cultivars

Abstract Two precocious yellow-fruited zucchinis (Cucurbita pepo L. cvs. Goldy and Gold Rush) and a green-fruited control, ‘Burpee Hybrid Zucchini9, were compared for yield and yield quality in a series of 6 trials conducted in Israel. Overall, ‘Goldy’ and ‘Burpee Hybrid Zucchini’ produced a similar number of fruits and yield of “Grade A’’ fruits, but ‘Gold Rush’ produced significantly less. Yield quality, expressed as the percentage of “Grade A” fruits, was higher in ‘Goldy’ and ‘Burpee Hybrid Zucchini’ than in ‘Gold Rush’. Off-color fruit production was the most important parameter which reduced yield quality in ‘Gold Rush’.