Active Zone Research Articles

Thoron, radon and microbial community as supportive indicators of seismic activity in groundwater

Earthquakes have a significant impact on groundwater environments as well as human life. However, identifying active and affected zones from seismic events using isotopic and microbial diversity indicators remains a challenging frontier. To validate the applicability of this coupled method for real-time analysis, we analyzed thoron (220Rn), radon (222Rn), microbial community compositions, and hydrochemistry in groundwater samples during the 2017 Pohang earthquake for the first time. We observed the detection of 220Rn in groundwater right before the aftershocks, with a high correlation to 222Rn concentrations. This indicates that 220Rn and 222Rn can serve as reliable seismic indicators for real-time analysis. The microbial data can assist in identifying affected groundwater zones, particularly when real-time detection of 220Rn is not feasible. At the phylum level, Peregrinibacteria and Firmicutes were only found in samples with detected thoron. At the genus level, hydrogen-oxidizing or sulfur-oxidizing bacteria could serve as indicators of active zones. Two statistical analyses, self-organizing map (SOM) and principal component analysis (PCA) using hydrochemical parameters, also correlated with the results from these coupled indicators. This study demonstrates the theoretical and practical applicability of 220Rn, 222Rn, and microbial community compositions as new multi-faceted ecological indicators, whether for real-time analysis or otherwise.

The geometry of fault reactivation and uplift along the central part of the Maacama Fault Zone, Northern California Coast Ranges (USA)

Fault reactivation of bedrock structures in active fault zones influences stress state and earthquake rupture phenomena through the introduction of weak slip surfaces that impact fault zone geometry and width. Yet, geometric relationships between modern faults and older reactivated faults are difficult to quantify in rocks that have experienced multiple deformation episodes. We used new geologic mapping, geomorphic tools, and structural modeling to quantify rock uplift and subsurface fault geometry of the central part of the Maacama Fault Zone near Ukiah, California, USA, and the surrounding area. Results suggest that the northern Mayacamas Mountains are in a tectonically driven disequilibrium, with differential rock uplift focused on the western side of the range. Steeply east-dipping fault surfaces and splays characterize the geometry of the Maacama Fault Zone. We mapped two newly identified faults to the east of the main Maacama Fault, the Cow Mountain–Mill Creek Fault, and Willow Creek Fault, which align with a moderately east-dipping cluster of microseismicity between 4–10 km depth beneath the Mayacamas Mountains. Static stress modeling on the Maacama Fault Zone and newly identified faults to the east quantify slip tendency values of 0.5–0.4, which suggests that the faults are moderately to poorly suited for slip in the modern stress field and may be weak. We infer that modern uplift is driven by oblique reverse, up-to-the-east, dip-slip motion on the reactivated Cenozoic Cow Mountain–Mill Creek and Willow Creek Faults as material is advected through a restraining bend on the Maacama Fault. This study shows that reactivated bedrock faults increase the fault zone width and introduce fault surfaces that contribute a component of vertical deformation and uplift in major strike-slip fault zones. Deformation is accommodated on an interconnected network of new and reactivated faults that delineate a complex seismic hazard.

Pre-Roman U-Th datings of an aqueduct near ancient Lebedos (Aegean Region, Türkiye)

In the vicinity of the ancient city of Lebedos, known as one of the twelve Ionian cities, located south of İzmir in W. Anatolia (Türkiye), the NE-SW-trending active Tuzla Fault zone is characterized by numerous hot springs and associated travertine-type carbonate deposits (sinter). Among these, the active Doğanbey bath features an approximately 560 m-long hot water aqueduct, called the “Roman Aqueduct”. This structure is distinct from the well-known Roman (Byzantine) ruins (Karakoç bath) in the area in terms of its materials and construction techniques. Despite the absence of detailed archaeological or geochronological studies in this region, the Doğanbey bath and aqueduct have conventionally been attributed to the Roman era.The trough and sidewalls of the Doğanbey aqueduct are covered with a 5–25 cm-thick, laminated sinter crust, formed by the flow of hot water. Each sinter lamina comprises radial structures of calcite and/or aragonite, resembling feather-like shrub structures. This study employs the U-Th chronometry to determine the age of sinter layers covering the Doğanbey bath aqueduct. Two layers from a single sinter sample of the ancient Doğanbey aqueduct yielded U-Th ages of 2717 ± 106 and 2528 ± 106 years (BP). These dates indicate a pre-Roman phase of settlement in the Lebedos area, a finding documented for the first time through this study.

Activity-dependent synapse clustering underlies eye-specific competition in the developing retinogeniculate system.

Co-active synaptic connections are often spatially clustered to facilitate local dendritic computations underlying learning, memory, and basic sensory processing. In the mammalian visual system, retinal ganglion cell (RGC) axons converge to form clustered synaptic inputs that enable local signal integration in the dorsal lateral geniculate nucleus (dLGN) of the thalamus. While visual experience promotes retinogeniculate synapse clustering after eye-opening, the earliest events in cluster formation prior to visual experience are unknown. Here, using volumetric super-resolution single-molecule localization microscopy and eye-specific labeling of developing retinogeniculate synapses in mice, we show that synaptic clustering is eye-specific and activity-dependent during retinogeniculate refinement in the first postnatal week. We identified a subset of retinogeniculate synapses with multiple active zones that are surrounded by like-eye synapses and depleted of synapse clustering from the opposite eye. In mutant mice with disrupted spontaneous retinal wave activity, synapses with multiple active zones still form, but do not exhibit the synaptic clustering seen in controls. These results highlight a role for spontaneous retinal activity in regulating eye-specific synaptic clustering in circuits essential for visual perception and behavior.

Tracing the Footsteps of Peace: Examining the Locations of UN Peacekeeping Patrols1

Recent studies have shown that United Nations (UN) peacekeepers are deployed to active conflict zones and are effective in reducing violence against civilians and between armed actors. Yet, while existing research has explored where peacekeepers are initially deployed, we know less about where peacekeepers patrol after deployment in which existing evidence remains largely anecdotal. We contend that UN peacekeeping patrols are generally conducted in areas where they are most needed in areas of armed violence and where civilians are targeted as UN peacekeepers are mandated to anticipate and respond to violence. We assess this argument using unique, geocoded mission report data compiled by the Joint Mission Analysis Center (JMAC) on UNAMID patrols across Darfur between January 2008 and April 2009. We find that, while UN patrols often stayed closer to the base, many patrols did venture far from base into the “sea of instability,” in locations with armed clashes and civilian violence.

Mechanisms and degradation pathways of tetracycline by Co7Fe3 nanoparticles anchored porous carbon activated peroxymonosulfate

In recent years, catalysts based on transition metals have been extensively employed for the activation of peroxymonosulfate (PMS) in the removal of tetracycline (TC) from aquatic environments. However, the efficiency of single transition metals and heterogeneous transition metals as catalysts has been somewhat limited. In this study, different bimetallic Co-Fe catalysts embedded in a porous carbon (Co7Fe3-500/600/700/800) were synthesized using a straightforward single-step calcination process at varying temperatures, addressing the issues of poor activation performance of single transition metals and low dispersion of active components in heterogeneous transition metals. The Co7Fe3-500/600/700/800 enhanced the electronic configuration, establishing electron-rich and highly active zones that promoted electron transfer between the Co and Fe transition metals. The results showed that Co7Fe3-600, exhibited excellent PMS activation performance, removing 95.1 % of TC from the solution within 10 min. Additionally, Co7Fe3-600 showed strong adaptability and resistance to interference under various environmental conditions. DFT calculations, HPLC-MS analysis and T.E.S.T analysis were used to predict potential degradation intermediates during TC degradation and assess their biological toxicity. This research introduces an innovative strategy for formulating sophisticated bimetallic alloy catalysts and contributes fresh perspectives on the mechanisms involved in the PMS activation process by bimetallic catalysts.

The role of gas emissions (He, Rn, and CO2) from fault zones in understanding fault and seismic activity

Active fault zones are critical pathways for the migration of deep fluids to the Earth’s surface, carrying gases such as He, Rn, and CO2 that provide evidence for the physical and chemical dynamics of the Earth’s interior. This review examines the geochemical characteristics of fault zone gases and their implications for understanding fault activity and seismic events. Fault zones with high activity levels exhibit significant gas release, and variations in soil and hot spring gas concentrations can serve as indicators of seismic activity. Changes in gas concentrations and isotopic ratios, particularly before and after earthquakes, reflect the dynamic interplay between deep-sourced and shallow-sourced fluids. Seismic-induced stress alterations enhance gas release along fault zones, leading to observable anomalies that can aid in earthquake monitoring and prediction. The study underscores the importance of isotope tracing in deciphering fluid sources, migration pathways, and the evolution of fault zones, providing valuable information for assessing tectonic activity and mitigating seismic risks.

Моделирование теплового режима дорожной одежды и земляного полотна лесовозных дорог

Soil freezing is observed throughout almost the entire forested area of the Russian Federarion in winter. The effect of negative temperatures on dusty-clay soils causes a number of adverse processes that change the properties of the soils themselves. One of the most unfavorable of these processes is the accumulation of moisture in soils under the influence of the movement of the freezing front. When freezing, water-saturated clay soils increase dramatically in volume. This leads to the appearance of frost heaving in the active zone of the forest roadbed, which has an extremely adverse effect on the structure of the entire pavement and can lead to damage to the pavement with a sharp deterioration in the transport and operational qualities of forest roads. To combat frost heaving, it is necessary to study the patterns of changes in the water-thermal regime of road structures. The depth of freezing of the pavement and the roadbed is of the greatest importance for predicting frost heaving and developing measures to combat this phenomenon. The article describes the developed system for monitoring the temperature of the road structure to a depth of 3 m and the measurement results which allow us to evaluate the temperature change at different depths from the road surface and determine the freezing depth. A total of 32 sensors have been installed with a step of 10 cm. A numerical simulation of the freezing process of the pavement and the upper part of the roadbed of a forest road has been performed, with the results compared with the indicators of field observations. Good data convergence has been revealed. According to the results of experimental studies, the freezing value has been 173 cm, and according to the results of numerical simulation – 190 cm. The average error in the results of numerical simulation of the freezing process of the pavement and the upper zone of the forest roadbed has been 8–10 % compared to the experimental data.

Geoparticipation as a tool for mapping calamities mosquito hotspots: A case study from Litovelské Pomoraví, Czechia

The coexistence of humans and nature has been a recurring theme throughout history, gaining particular importance in the context of climate change and the sustainability of our planet for future generations. However, the delicate balance between humans and nature is often disrupted, as exemplified by the Litovelské Pomoraví Protected Landscape Area in Czechia, where close interaction between humans and mosquitoes is a persistent issue. In this area, when environmental conditions are favorable for mosquito outbreaks, the local population living near the floodplain forests experiences significant discomfort for extended periods. The risk of mosquito overpopulation has intensified in recent years, largely attributed to climate change. Rising water temperatures in breeding habitats accelerate the developmental cycles of certain mosquito species, shortening the time required for maturation. This study aims to utilize modern geoinformation techniques to assess mosquito activity within the Litovelské Pomoraví region and evaluate the perceived impact on human populations. The methodological approach integrates the development of a representative population distribution layer and participatory mapping. These efforts culminate in the identification of active mosquito zones and the quantification of the at-risk population. While long-term data indicate that the situation is not deteriorating significantly, the study confirms that elevated mosquito activity continues to disrupt the daily lives of residents and visitors. Notably, summer mosquito outbreaks exert a broader impact than spring outbreaks, affecting both a larger geographic area and a greater proportion of the population.The study's primary output, the spatial delineation of active mosquito zones and the identification of continuously populated areas at risk, holds significant value for the integrated management of the region. These findings can help mitigate the annual occurrence of mosquito outbreaks, improve the health of the floodplain forest ecosystem, and promote biodiversity conservation.

Paraventricular hypothalamic RUVBL2 neurons suppress appetite by enhancing excitatory synaptic transmission in distinct neurocircuits

The paraventricular hypothalamus (PVH) is crucial for food intake control, yet the presynaptic mechanisms underlying PVH neurons remain unclear. Here, we show that RUVBL2 in the PVH is significantly reduced during energy deficit, and knockout (KO) of PVH RUVBL2 results in hyperphagic obesity in mice. RUVBL2-expressing neurons in the PVH (PVHRUVBL2) exert the anorexigenic effect by projecting to the arcuate hypothalamus, the dorsomedial hypothalamus, and the parabrachial complex. We further demonstrate that PVHRUVBL2 neurons form the synaptic connections with POMC and AgRP neurons in the ARC. PVH RUVBL2 KO impairs the excitatory synaptic transmission by reducing presynaptic boutons and synaptic vesicles near active zone. Finally, RUVBL2 overexpression in the PVH suppresses food intake and protects against diet induced obesity. Together, this study demonstrates an essential role for PVH RUVBL2 in food intake control, and suggests that modulation of synaptic plasticity could be an effective way to curb appetite and obesity.

Single-vesicle imaging reveals actin-dependent spatial restriction of vesicles at the active zone, essential for sustained transmission

Synaptic-vesicle (SV) recruitment is thought to maintain reliable neurotransmitter release during high-frequency signaling. However, the mechanism underlying the SV reloading for sustained neurotransmission at central synapses remains unknown. To elucidate this, we performed direct observations of SV reloading and mobility at a single-vesicle level near the plasma membrane in cerebellar mossy fiber terminals using total internal reflection fluorescence microscopy, together with simultaneous recordings of membrane fusion by capacitance measurements. We found that actin disruption abolished the rapid SV recruitment and reduced sustained release. In contrast, induction of actin polymerization and stabilization did not affect vesicle recruitment and release, suggesting that the presence of actin filaments, rather than actin dynamics, was required for the rapid recruitment. Single-particle tracking experiments of quantum dot-labeled vesicles, which allows nanoscale resolution of vesicle mobility, revealed that actin disruption caused vesicles to diffuse more rapidly. Hidden Markov modeling with Bayesian inference revealed that SVs had two diffusion states under normal conditions: free-diffusing and trapped. After disruption of the actin filament, vesicles tended to have only the free-diffusing state. F-actin staining showed that actin filaments were localized outside the active zones (AZs) and surrounded some SV trajectories. Perturbation of SV mobility, possibly through interference with biomolecular condensates, also suggested that the restricted diffusion state determined the rate of SV recruitment. We propose that actin filaments confined SVs near the AZ to achieve rapid and efficient recruitment followed by priming and sustained synaptic transmission.

Pre-fusion AAA+ remodeling of target-SNARE protein complexes enables synaptic transmission.

Membrane fusion is driven by SNARE complex formation across cellular contexts, including vesicle fusion during synaptic transmission. Multiple proteins organize trans-SNARE complex assembly and priming, leading to fusion. One target membrane SNARE, syntaxin, forms nanodomains at the active zone, and another, SNAP-25, enters non-fusogenic complexes with it. Here, we show that the AAA+ protein NSF (N-ethylmaleimide sensitive factor) and SNAP (soluble NSF attachment protein) must act prior to fusion. We show that syntaxin clusters are conserved, that NSF colocalizes with them, and characterize SNARE populations within and near these clusters using cryo-EM. Supercomplexes of NSF, α-SNAP, and either a syntaxin tetramer or two binary complexes of syntaxin-SNAP-25 reveal atomic details of SNARE processing and show how sequential ATP hydrolysis drives disassembly. These results suggest a functional role for syntaxin clusters as reservoirs and a corresponding role for NSF in syntaxin liberation and SNARE protein quality control preceding fusion.

Failure Mechanism and Control Technology of the Primary Support for Asymmetric Deformation Disaster of Deep Tunnel

Asymmetric large deformation disasters inevitably occur when highway tunnels cross active fault zones. Relying on the Nanlangshan No.1 Tunnel Project, the mechanism of asymmetric deformation is analyzed based on the in-situ engineering geology and tunnel monitoring data, and numerical calculations are used for verification. Based on the results of asymmetric large deformation disaster analysis, the control scheme of double-layer primary support is proposed, and the design is optimized with numerical calculation before engineering practice. The conclusions are as follows: 1. The original primary support program cannot withstand the asymmetric surrounding rock pressure, and the slip of the weak interlayer in the engineering geology is the main reason for the asymmetric deformation disaster in the tunnel. 2. According to the results of the numerical calculations, the tilted weak interlayer greatly reduces the stability of the surrounding rock, which leads to the local bending damage in the left shoulder of the tunnel, and the local shear deformation damage in the right arch girdle. 3. When using the double-layer primary support program, the appropriate delay of support timing for the second layer of primary support can effectively decrease the tunnel asymmetric deformation. However, if the second layer of primary support lag distance is too large, it will also lead to tunnel deformation convergence larger, or support failure and other issues. So, it is recommended that the second layer of primary support lags behind the first layer of primary support by 2 m to be applied.

Dysregulation of protein SUMOylation networks in Huntington's disease R6/2 mouse striatum.

Huntington's disease (HD) is a neurodegenerative disorder caused by an expanded CAG repeat mutation in the Huntingtin (HTT) gene. The mutation impacts neuronal protein homeostasis and cortical/striatal circuitry. SUMOylation is a post-translational modification with broad cellular effects including via modification of synaptic proteins. Here, we used an optimised SUMO protein-enrichment and mass spectrometry method to identify the protein SUMOylation/SUMO interaction proteome in the context of HD using R6/2 transgenic and non-transgenic (NT) mice. Significant changes in enrichment of SUMOylated and SUMO-interacting proteins were observed, including those involved in presynaptic function, cytomatrix at the active zone scaffolding, cytoskeleton organization, and glutamatergic signaling. Mitochondrial and RNA-binding proteins also showed altered enrichment. Modified SUMO-associated pathways in HD tissue include clathrin-mediated endocytosis signaling, synaptogenesis signaling, synaptic long-term potentiation, and SNARE signaling. To evaluate how modulation of SUMOylation might influence functional measures of neuronal activity in HD cells in vitro, we utilised primary neuronal cultures from R6/2 and NT mice. A receptor internalization assay for the metabotropic glutamate receptor 7 (mGLUR7), a SUMO enriched protein in the mass spec, showed decreased internalization in R6/2 neurons compared to NT. siRNA-mediated knockdown of the E3 SUMO ligase Protein Inhibitor of Activated STAT1 (Pias1), which can SUMO modify mGLUR7, prevented this HD phenotype. In addition, microelectrode array analysis of primary neuronal cultures indicated early hyperactivity in HD cells, while later timepoints demonstrated deficits in several measurements of neuronal activity within cortical neurons. HD phenotypes were rescued at selected timepoints following knockdown of Pias1. Collectively, our results provide a mouse brain SUMOome resource and show that significant alterations occur within the post-translational landscape of SUMO-protein interactions of synaptic proteins in HD mice, suggesting that targeting of synaptic SUMO networks may provide a proteostatic systems-based therapeutic approach for HD and other neurological. Disorders.

Does the direct effect of friction increase continuously with absolute temperature?

Constitutive models of fault friction form the basis of physics-based simulations of seismic activity. A generally accepted framework for the slip-rate and state dependence of friction involves a thermally activated process, whereby the probability of slip along microasperities adheres to an Arrhenius law. This model, which has become widely adopted among experimentalists and theoreticians, predicts a continuous increase of the direct effect with absolute temperature, but is it observed experimentally? Leveraging comprehensive laboratory data across diverse hydrothermal, barometric, and lithological conditions, we demonstrate that, contrary to the classical view, the direct effect for a given deformation mechanism remains largely temperature-independent. Instead, the incremental shifts in the direct effect often coincide with the brittle to semi-brittle transition, across which distinct deformation mechanisms operate. These considerations challenge the validity of the classical model. Realistic constitutive laws for rock failure within the lithosphere must incorporate the contributions of multiple deformation mechanisms within active fault zones.

Detecting and sourcing GHGs and atmospheric trace gases in a municipal waste treatment plant using coupled chemistry and isotope compositions

Landfill operations and waste processing facilities are important and highly heterogeneous sources of both greenhouse gases (GHGs) and non-GHG air pollutants in the atmosphere. This arises the need for detailed apportionment of waste sources in order to locate and subsequently reduce emissions from landfills. Here, a time series of in situ measurements of atmospheric trace gases and spatial allocation of specific emission source types under different processing phases and environmental conditions were conducted in and in the surroundings of a Municipal Solid Waste Treatment Plant (MSWTP) in south-western Poland. Results revealed that several individual GHG sources dominated across the waste processing facility and that GHGs concentrations displayed spatial seasonality. An increase in the ground-level CH4 concentrations, from ∼ 30.3 to 56.3 ppmv, was observed close (∼5 – 10 m) to the major emission sources within the MSWTP. While hotspot areas generally yielded elevated CH4 concentrations near the soil surface, these were relatively low (2.4 to 8.9 ppmv) along the facility’s fence line. The study of the corresponding δ13C delineated the extent of dispersion plumes downwind emission hotspots, characterized by a 13C depletion (around 4.0 ‰) in the atmospheric CH4 and CO2. For CH4, emissions were isotopically discriminated between the extraction wells at active quarters/cells (δ13C = –58.3 ± 1.1 ‰) and biogas produced in the biological waste treatment installation (δ13C = –62.7 ± 0.7 ‰). Most of the trace compounds (non-methane hydrocarbons, halocarbons, oxygen-bearing organic gases, ketones, nitrogenous and sulphurous gases, and other admixture compounds) detected at the ground surface were linked to the CH4- and CO2-rich spots. Despite the relatively high variability in the concentrations of organic and inorganic compounds observed at the MSWTP active zones, our results suggest that they do not have a meaningful impact on the surrounding air quality.

Analysis of the interactions between laser and arc due to different geometric arrangements and laser parameters in the additive manufacturing of Ti-6Al-4V

Additive manufacturing based on arc welding (=DED-Arc) has already established itself in the world of additive manufacturing. Recent process optimizations show the increased use in a hybrid process by irradiating an additional laser beam into the active arc zone. Numerous publications can be found in the literature for steel, aluminum, or bronze, in which, among other things, a strength-enhancing effect or an improved wall surface during this hybrid process is reported. The way in which the laser beam impacts the arc, at what distance, at what angle and with what spot size or focusing appears to be random in the publications and is always chosen differently in the literature. In order to close this knowledge gap, a corresponding holder for a DED-Arc torch and laser optic is developed, which allows these geometric factors to be systematically changed and also enables the titanium alloy Ti-6Al-4V to be processed in a hybrid process. By welding individual tracks and walls with different angles, distances and spot sizes of the two energy sources, a fundamental understanding of the laser-arc hybrid process is created. The results are analyzed using a high-speed camera and evaluated on the basis of surface measurements, micrographs, and power source values. This showed that the distance between the laser and the arc has the greatest effect on the additive process stability and the arc power sources values. In the range of 2–6 mm, there are, in some cases, strong fluctuations in the wire deposition and the arc voltage, so that this range should be avoided. It also showed that a wider spot sizes range of 360–600 μm can be used, whereby different ranges are recommended depending on the target size of uniform wall height or thickness. For the laser incidence angle, the most uniform walls across all tests are achieved at 20°. As the angle of incidence becomes flatter, the walls become more irregular, although there is no linear relationship or areas that should be avoided at all.

Сейсмотектоническая активность Ляховичского разлома в Припятском прогибе

The main result of the work was the identification of the present-day seismotectonic regime of an active zone of the Lyakhovichi regional fault. The areal and space-time distribution of the earthquake epicenters along the fault line was reconstructed. The coordinates of the seismically active fault zone located within an area of 52.738—53.037° N and 26.830—28.034° E were determined. It was found that the distribution of earthquakes along the fault line occurred non-uniformly in space and time. The period from 2012 till 2021 was the most active one by the quantity of occurred earth quakes (169) and released total seismic energy (4.5929•109 J). The strongest earthquake with a magnitude M = 2.9 was recorded in the eastern part of the fault on September 03, 2021. Our reconstructions determined the value of seismic dislocation along the fault, which corresponded to the kinematic slip fault. To characterize the modern seismogeodynamic activity in the Lyakhovichi fault zone, a comprehensive technique based on long-term monitoring of seismic events was used. Based on the performed studies, the seismotectonic potential of the seismoactive zone of the Lyakhovichi fault was determined.

Enhancing trauma care in Ukraine amid conflict: A successful implementation of the modified advanced trauma life support course in an active war zone.

Following the 2022 Russian invasion, Ukraine's healthcare system suffered extensive damage, with over 1000 medical facilities destroyed, exacerbating the trauma care crisis. The absence of standardized trauma training left Ukrainian healthcare providers ill-equipped to manage the surge in trauma cases amid conflict. To bridge this gap, we implemented advanced trauma life support (ATLS) courses in Ukraine amid active warfare, aiming to enhance trauma care expertise among healthcare professionals. A consortium, including the International Medical Corps, Harvard Humanitarian Initiative, and others, responded to a request from the Ukrainian Ministry of Health. The ATLS curriculum, translated into Ukrainian, guided the training, with US-based instructors sent to Ukraine for teaching. Despite logistical challenges, such as missile attacks and curfews, the courses ran in multiple Ukrainian cities over 3months. Course effectiveness was evaluated through pre- and post-course knowledge tests, self-efficacy surveys, and satisfaction assessments. Ten ATLS courses trained 213 Ukrainian healthcare providers across five deployments. Significant improvements in knowledge scores (p<0.05) and enhanced self-reported confidence in trauma management were observed. Notably, no casualties were reported among instructors or students, highlighting program safety despite security challenges. Our study demonstrates successful ATLS course implementation in an active war zone, filling a critical gap in trauma education in Ukraine. Despite challenges, the program significantly enhanced participants' trauma care knowledge and confidence. Collaboration between international and local partners was pivotal. This model can serve as a valuable framework for trauma education globally, improving outcomes in conflict zones and resource-limited settings.

Kif1a and intact microtubules maintain synaptic-vesicle populations at ribbon synapses in zebrafish hair cells.

Sensory hair cells of the inner ear utilize specialized ribbon synapses to transmit sensory stimuli to the central nervous system. This transmission necessitates rapid and sustained neurotransmitter release, which depends on a large pool of synaptic vesicles at the hair-cell presynapse. While previous work in neurons has shown that kinesin motor proteins traffic synaptic material along microtubules to the presynapse, the mechanisms of this process in hair cells remain unclear. Our study demonstrates that the kinesin motor protein Kif1a, along with an intact microtubule network, is essential for enriching synaptic vesicles at the presynapse in hair cells. Through genetic and pharmacological approaches, we disrupt Kif1a function and impair microtubule networks in hair cells of the zebrafish lateral-line system. These manipulations led to a significant reduction in synaptic-vesicle populations at the presynapse in hair cells. Using electron microscopy, in vivo calcium imaging, and electrophysiology, we show that a diminished supply of synaptic vesicles adversely affects ribbon-synapse function. Kif1aamutants exhibit dramatic reductions in spontaneous vesicle release and evoked postsynaptic calcium responses. Furthermore, kif1aamutants exhibit impaired rheotaxis, a behaviour reliant on the ability of hair cells in the lateral line to respond to sustained flow stimuli. Overall, our results demonstrate that Kif1a-mediated microtubule transport is critical to enrich synaptic vesicles at the active zone, a process that is vital for proper ribbon-synapse function in hair cells. KEY POINTS: Kif1a mRNAs are present in zebrafish hair cells. Loss of Kif1a disrupts the enrichment of synaptic vesicles at ribbon synapses. Disruption of microtubules depletes synaptic vesicles at ribbon synapses. Kif1aa mutants have impaired ribbon-synapse and sensory-system function.