Starch Gel Zone Electrophoresis Research Articles

Plasma cells and serum proteins in marine fish.

Summary1) Serum proteins of 18 species of fish have been analyzed by means of starch gel zone electrophoresis. In 10 of these species, observations were also made on occurrence of plasma cells in spleens. 2) Elasmobranchs had typical plasma cells in their spleens and proteins in their serum migrating like gamma globulins upon electrophoresis. Teleosts, however, generally had very few plasma cells in their spleens and no demonstrable gamma globulin in the serum. These observations suggest that among marine fish, there may be an association between plasma cells and gamma globulins.

Histochemical demonstration of enzymes separated by zone electrophoresis in starch gels.

Histochemical demonstration of enzymes separated by zone electrophoresis in starch gels.

Histochemical Demonstration of Enzymes Separated by Zone Electrophoresis in Starch Gels

Histochemical Demonstration of Enzymes Separated by Zone Electrophoresis in Starch Gels

Histochemical Demonstration of Enzymes Separated by Zone Electrophoresis in Starch Gels

Histochemical Demonstration of Enzymes Separated by Zone Electrophoresis in Starch Gels

Two-dimensional electrophoresis of serum proteins.

A METHOD of zone electrophoresis in starch gels has recently been described by one of us1. The high degree of resolution obtained with this method when applied to serum appears to be due to the use of a supporting medium the pore size of which approaches the molecular dimensions of some of the proteins involved, so that resolution by molecular sizes is superimposed on resolution by free solution mobilities. Consequently the starch-gel electrophoretic separations result from a qualitatively different process from that involved in free-solution electrophoresis. A combination of the two electrophoretic processes at right-angles should therefore give a much greater degree of resolution than is possible with either separately. Such a two-dimensional electrophoretic system has been tested by us, using electrophoresis on filter paper as a close approximation to that occurring in free solution. The present communication illustrates the type of result which can be obtained; a detailed description of the method will be published later.

Zone electrophoresis in starch gels: group variations in the serum proteins of normal human adults.

Zone electrophoresis in starch gels: group variations in the serum proteins of normal human adults.