ZnSO4 Groups Research Articles

Study on Zn relative concentration and chemical state in broilers duodenum by micro-X-ray fluorescence and micro-X-ray absorption fine structure

Micro-X-ray fluorescence (μ-XRF) and micro-X-ray absorption fine structure (μ-XAFS) are not widely used in animal science. The objective of this experiment was to employ μ-XRF and μ-XAFS technique to determine the change of Zn quantity, distribution and chemical state (Zn2+ chemical state, organic zinc chemical state) into intestinal wall using different zinc sources. 45 newly hatched healthy (1-d-old) commercial male broilers were used in this experiment. The chicks were fed a corn–soybean meal basal diet (90.50mg/kg) from day 1 to 21, but were fed a semipurified diet (12.51mg/kg) after day 21 to deplete the body Zn stores. At 28d of age, after an overnight fast, 45 birds were randomly allotted to 3 perfusion groups (ZnMet, ZnLys, ZnSO4) with 15 replicates/group for in situ ligated intestinal loops of broilers in experiment. Duodenum of each bird was used as 1 replication of intestinal segments. The solutions injected into the duodenal loops were buffered with 15.5mmol/L of morpholineoethanesulfonic acid. In the treatment groups of different Zn sources, 0.616mmol/L (40mg/L) was added to the media. The 3.5mL of Zn dose was injected and incubated 30min in the abdomen cavity. The μ-XRF and μ-XAFS were used to analyze the relative quantity, distribution and chemical state of Zn in the intestinal wall, and atomic absorption spectrometry (AAS) was used to verify Zn concentration in the whole intestinal sac. The results showed that ZnMet and ZnLys group samples have a greater amount of zinc in the intestinal wall specific region than ZnSO4 group. The Zn chemical state of organic Zn and ZnSO4 group were identical in intestinal wall specific region. In addition, the Zn concentration achieved using ZnMet was greater than that for ZnLys and ZnSO4 (P<0.05) as measured by AAS. As observed in this experiment, organic zinc was more easily absorbed than inorganic Zn.

Possibility of Alveolar Bone Promoting Enhancement by Using Lipophilic and/or Hydrophilic Zinc Related Compounds in Zinc-Deficient Osteoporosis Rats

The purpose of this research is improvement of therapeutic treatment for periodontitis by using lipophilic and/or hydrophilic zinc materials. The sample suspension injections were prepared from zinc octanate (C8Zn), zinc stearate (C18Zn), zinc sulfate hepta-hydrate (ZnSO4) and tricalcium phosphate (ZnTCP) containing 6.17 w/w% zinc. After administrating of all injections to around alveolar bone of zinc-deficient osteoporosis rats, plasma Zn concentration, bone mineral content (BMC) of jawbone, BMC and bone mechanical strength (BMS) of femur and permeability tests for hairless rat stripped skin were measured as therapeutic scores. BMC and BMS were measured by using an X-ray computing tomography and the three-point bending method, respectively. The body weight, plasma Zn concentrations and the area under curve (AUC) for Zn of C8Zn, C18Zn and ZnTCP group rats were higher than those of control group, but those of ZnSO4 group were not changed. BMC of alveolar bone and femur and BMS of femur for C8Zn and C18Zn groups for 12 weeks were significantly higher than those of the control group, but those of ZnSO4 group were not changed. Stripped rat skin permeability treated by the hydrophilic creams containing C8Zn was 5-times higher than that of ZnTCP.

Effect of Zinc‐ligand Complexes on Intracellular Uptake and Distribution in the Urinary System of Male Sprague‐Dawley Rats

Zinc involves in many cellular metabolisms including gene expression, carcinogenesis, and etc. Especially, prostate gland and testis contain high level of zinc, which is associated with genitals related diseases. We carried out this study to examine the zinc concentration in plasma and various organs in Zn-ligand complexes and ZnSO4 administered rats, and to investigate the mRNA expression level of zinc transporter. SD rats were divided into three groups: ZnSO4, Zn-Arg and Zn-Asp. After oral administration of zinc substrates (40mg zinc/kg body weight), blood and organs were collected. The zinc concentration was detected by zinquin, and zinc transporter mRNA expression was measured by semi quantitative RT-PCR. We observed that plasma zinc concentration was increased after zinc administration. Plasma zinc concentration of Zn-Arg and Zn-Asp group was peaked out at 2h after zinc administration. Although intracellular zinc level in prostate gland and testis is relatively lower than in other organs, zinc level of Zn-Asp group is higher than other two groups. Also, we found that ZnT2 mRNA expression level was higher in Zn-Asp group than in Zn-Arg and ZnSO4 group. This study shows Zn-ligand complexes are effective the uptake and accumulation of zinc in prostate gland and testis. Therefore, zinc-ligand complex can be used for available therapy of many zinc-related diseases of the male genitals.

EFFECT OF ZINC SULPHATE AND ZINC METHIONINE ON GROWTH, PLASMA GROWTH HORMONE CONCENTRATION, GROWTH HORMONE RECEPTOR AND INSULIN‐LIKE GROWTH FACTOR‐I GENE EXPRESSION IN MICE

1. The current experiment was conducted to investigate the effect of zinc sulphate (ZnSO4) and zinc methionine (Zn-Met) on growth and their effect on plasma growth hormone (GH) concentration, growth hormone receptor (GHR) and insulin-like growth factor I (IGF-I) mRNA expression in mice. 2. Ninety male KunMing (KM) mice were randomly divided into three treatments. The control group was fed on a basal diet containing 11.67 mg/kg of zinc. The ZnSO4 group and Zn-Met group were fed on the diets supplemented with ZnSO4 or Zn-Met at 30 mg/kg (containing zinc of 40.05 and 40.75 mg/kg, respectively). The mice were offered the test diets for 10 days. Weight gains and food intake were measured at the end of the experiment, zinc contents in liver and serum were determined using atomic absorption spectrophotometry; GH was determined by radioimmunoassay, the levels of GHR and IGF-I mRNA were determined with reverse transcript polymerase chain reaction. 3. Both ZnSO4 and Zn-Met enhanced weight gain and food intake in the mice, Zn-Met improved the growth and food intake more effectively than ZnSO4 did (P < 0.05). The both forms of zinc had no effect on GH and the level of GHR mRNA expression (P > 0.05) and they up-regulated the expression of IGF-I mRNA (P < 0.05). As compared to ZnSO4, Zn-Met enhanced the level of IGF-I mRNA significantly (P < 0.05). 4. Both ZnSO4 and Zn-Met had no effect on plasma GH and the expression of GHR mRNA, but they enhanced the expression of IGF-I mRNA. Zinc methionine enhanced the weight gain and up-regulated IGF-I mRNA expression more effectively than ZnSO4.

Zinc salts precipitate unconjugated bilirubin in vitro and inhibit enterohepatic cycling of bilirubin in hamsters.

We have evidence for enterohepatic cycling of bilirubin experimentally and in vivo in humans. This study was designed to investigate whether Zn salts might inhibit such cycling of bilirubin. Micellar bile salt solutions with unconjugated bilirubin were prepared, appropriate concentrations of Zn salts were added, and unconjugated bilirubin precipitation was measured. Hamsters and Wistar rats were fed a chow diet or a chow diet enriched with 1% ZnSO4, and bilirubin secretion rates were monitored. Unconjugated bilirubin was precipitated maximally (90%) after a 10-min incubation with 5 mM Zn salts in the pH range of 6.8-9.0. In control hamsters, total bilirubin secretion rates into bile were 36.0 +/- 2.8 nmol h(-1) 100g(-1) body weight, whereas they were 25.0 +/- 3.3 nmol h-1 100(-1) g in the ZnSO4 group (P < 0.05). Zn salts that flocculate at physiological pH adsorb unconjugated bilirubin almost completely from unsaturated micellar BS solutions. In addition, Zn salts administered orally suppress biliary bilirubin secretion rates in hamsters. These findings suggest that the administration of Zn salts may inhibit the enterohepatic cycling of unconjugated bilirubin in humans who are predisposed to pigment gallstone formation due to diet, disease or drugs.