Zingiber Officinale Roscoe Rhizome Research Articles

Effect of Orijin Bitters, Aqueous Extracts of Hibiscus Sabdariffa Calyx and Zingiber Officinale Roscoe Rhizome on the Liver of adult wistar rats

BACKGROUND AND AIM: Orijin bitters, Hibiscus sabdariffa and Zingiber officinale roscoe are consumed for medicinal properties and their effects were evaluated in this study on adult wistar rats’ liver. METHODOLOGY: Thirty rats were divided into 6 groups, 5 in each. Group 1 received distilled water, group 2, 70cl/70kg/bw orijin bitters, group 3 orijin bitters and 200mg/kg/bw Hibiscus sabdariffa calyx aqueous extract, group 4 orijin bitters and 500mg/kg/bw Hibiscus sabdariffa calyx aqueous extract, group 5 orijin bitters and 200mg/kg/bw officinale rhizome aqueous extract, group 6 orijin bitters and 500mg/kg/bw Zingiber officinale rhizome aqueous extract. After administration, the rats were sacrificed and the liver harvested for biochemical and histological analysis. RESULTS: Aspartate aminotransferase and alkaline phosphate increased in group 2 while alanine transaminase decreased. Aspartate aminotransferase and alanine transaminase increased in most treatment groups while alkaline phosphate decreased. P < 0.05 was considered statistically significant. Orijin bitters distorted the liver tissue while Hibiscus sabdariffa and Zingiber officinale roscoe extracts resulted in restoration. CONCLUSION: Orijin bitters may possess toxicity to the liver. Hibiscus sabdariffa calyx and Zingiber officinale roscoe rhizome due to antioxidants present ameliorated the effect.

Chemodiversity of Zingiber officinale Roscoe rhizome essential oil at different drying times

Ginger (Zingiber officinale), a globally distributed plant, is widely used in the industry for its flavourings, seasonings, and beverages. However, maintaining its quality and volatile components during processing has posed a challenge. This study, therefore, aimed to assess the impact of drying time (24, 48, and 72h) in a circulation oven at 40 °C on the chemical composition and yield of fresh and dried ginger. The essential oils were extracted using the hydrodistillation method, and their chemical analysis was conducted using gas chromatography. The drying time in the oven directly influenced the essential oil yield, with a longer time resulting in a higher yield. We identified 27 compounds in the essential oils, varying their predominance depending on the drying time. The PCA analysis revealed that the drying time can lead to the formation of different chemotypes for ginger, indicating that altering the drying time can yield significantly different chemical profiles.

Development of a determination method for quality control markers utilizing metabolic profiling and its application on processed Zingiber officinale Roscoe rhizome.

This study established an Orthogonal Partial Least Squares (OPLS) model combining 1H-NMR and GC-MS data to identify characteristic metabolites in complex extracts. Both in metabolomics studies, and natural product chemistry, the reliable identification of marker metabolites usually requires laborious isolation and purification steps, which remains a bottleneck in many studies. Both ginger (GR) and processed ginger (PGR) are listed in the Japanese pharmacopeia. The plant of origin, the rhizome of Zingiber officinale Roscoe, is differently processed for these crude drugs. Notably, the quality of crude drugs is affected by genetic and environmental factors, making it difficult to maintain a certain quality standard. Therefore, characteristic markers for the quality control of GR and PGR are required. Metabolomic analysis using 1H-NMR was able to discriminate between GR and PGR, but there were unidentified signals that were difficult to distinguish based on NMR data alone. Therefore, we combined 1H-NMR and GC-MS analytical data to identify them by OPLS. As a result, αr-curcumene was found to be a useful marker for these identifications. This new approach enabled rapid identification of characteristic marker compounds and reduced the labor involved in the isolation process.

Isolation of 6-gingerol and semi-synthesis of 1,4-benzodiazepines derivatives: An in-situ pharmacokinetics properties, molecular docking and molecular dynamics simulation assessments

This paper outlines a methodical approach for isolating 6-gingerol (1a) from Zingiber officinale Roscoe rhizomes on a gram-scale, resulting in a product of high purity and significant yield. Further, 6-gingerol (1a) [SSG1] derivatives, including 1-(4-hydroxy-3-methoxyphenyl)decane-3,5-dione (1ab), were synthesized via a semi-synthetic pathway involving DMP-mediated fast oxidation and replication. Subsequently, a new series of 1,4-benzodiazepines (3a-c) was synthesized quantitatively using a basic technique. This synthesis necessitated the interaction of 1ab with various o-phenylenediamine (2a-c) compounds. Spectroscopic methods were employed to characterize the synthesized 1,4-benzodiazepines (3a-c)[SSG2, SSG3 & SSG4]. Despite extensive investments by pharmaceutical companies in traditional drug research and development for diseases like type 2 diabetes (T2D), successful treatments remain elusive. Medication repurposing has gained traction as a strategy to address not only diabetes but also other disorders. Leveraging existing molecular pharmacology data accelerates the development of new medications. This paper underscores the importance of repurposing traditional medicines to combat a range of communicable and non-communicable diseases, offering a promising avenue for therapeutic advancement. Additionally, molecular docking studies suggested that one derivative (SSG2) exhibited stronger binding affinity compared to the reference standards. Overall, the findings of this study highlight the potential of semi-synthetic gingerol derivatives for the development of novel therapeutic agents.

Evaluation of the efficacy and safety of a herbal formulation for rheumatoid arthritis – A non-inferiority randomized controlled trial

Ethnopharmacological relevanceRheumatoid arthritis (RA) is a diverse disease characterized by a variable, progressive course of articular and extra-articular symptoms that are linked with pain, disability, and mortality. The exact cause of rheumatoid arthritis is still being investigated, and there is no cure for this debilitating, persistent, painful disease. Qurs-e-Mafasil, a herbal Unani preparation, is regarded as a potent treatment for Waja'al-Mafasil, a condition clinically similar to rheumatoid arthritis, but scientific evidence is scarce. Aim of the studyThis study aimed to assess the non-inferiority of Qurs-e-Mafasil compared to celecoxib in the treatment of patients with rheumatoid arthritis. Materials and methodsThis randomized controlled trial was conducted on seventy patients diagnosed with rheumatoid arthritis between the ages of 35 and 55 years. The participants were randomly allocated in a ratio of 3:2, with 42 participants in the test group and 28 participants in the control group. The test group was administered 2 tablets (each having 500 mg) of Qurs-e-Mafasil, while the control group was administered 1 capsule of Celecoxib 100 mg. Both medications were delivered for four weeks. The primary outcome measure was European League Against Rheumatism (EULAR) response criteria based on Disease Activity Score-28 (DAS28) assessed before and after therapy, whereas the secondary outcome measure was the change in joint pain severity as determined by a 100 mm Visual Analog Scale (VAS) at baseline and each follow-up. The safety of the interventions was evaluated based on adverse event monitoring at each follow-up and laboratory tests including hemogram, Liver Function Tests (LFTs), Kidney Function Tests (KFTs), and a complete urine examination performed at baseline and after four weeks of treatment. ResultsThe per-protocol analysis was done on 50 participants (30 in test group and 20 in control group) who completed the study duration. Thus, at the conclusion of the trial, participants in the test and control groups had either a moderate or no response based on EULAR response criteria. The odds ratio for no response versus moderate response between the test and the control groups was 0.71 (95% CI: 0.20–2.55) with p = 0.744. Moreover, the observed mean differences in VAS scores between the test and the control groups at 1st, 2nd, 3rd, and final follow-up were −0.33 (95% CI: −6.65 to 5.99, p = 0.916), 0.50 (95% CI: −5.63 to 6.63, p = 0.870), 2.42 (95% CI: −2.95 to 7.78, p = 0.370), and 3.00 (95% CI: −1.82 to 7.84, p = 0.219), respectively. ConclusionsThe differences in primary and secondary outcomes between the two groups indicate that Qurs-e-Mafāsil, a herbal Unani formulation containing Zingiber officinale Roscoe rhizome, Colchicum luteum Baker root, Piper nigrum L. fruit, and Withania somnifera (L.) Dunal. root, is comparable to celecoxib in the treatment of rheumatoid arthritis.

Biological control of fruit rot of postharvest orange (Citrus aurantium) by aqueous plant extracts

Post-harvest rot symptoms caused by complex airborne fungi are nowadays the most serious threatening disease affecting orange fruits in Nigeria and presumably in many African countries. Experimental trials were conducted at the Department of Biological Sciences (Akwa Ibom State, Nigeria) from January to December 2022 to ascertain the pathogens associated with postharvest fruit rot of oranges (Citrus aurantium L.). Results of the pathogenic test revealed that Fusarium sp. and Penicillium sp. are pathogenic and produced the highest lesion diameter (38.67 and 40.33 mm, respectively) and disease severity index (88.67 and 94.33%, respectively). The frequency occurrence of Fusarium sp. (69.14%) and Penicillium sp. (50.62%) was the highest. Two aqueous extracts of Azadirachta indica Juss. leaves and Zingiber officinale Roscoe rhizomes (at 80% concentration) were evaluated for their antifungal activities against the pathogenic isolates under in vitro and in vivo conditions. The results revealed that A. indica and Z. officinale aqueous extracts exhibited the highest mycelial growth inhibition (>90%) of Fusarium sp. and Penicillium sp. Similarly, under in vivo assay minimum lesion diameter (≤ 6.37 mm) and disease severity index (≤11.13%) were recorded in these aqueous extracts. In conclusion, the application of A. indica and Z. officinale can provide an alternative to fungicides against Penicillium sp. + Fusarium sp.

Zingiber officinale Roscoe Rhizome Extract Exerts Senomorphic and Anti-Inflammatory Activities on Human Endothelial Cells

Aging is related to a low-grade and sterile inflammation called inflammaging, recognized as the main risk factor for age-related disease (ARD) development. Inflammaging is fostered by the repeated activation of immune cells, as well as by the accumulation of senescent cells. Recently, a number of natural compounds have gained attention to be tested as anti-aging therapies, based on their anti-inflammatory activity and/or ability to reduce the pro-inflammatory secretome of senescent cells (senomorphyc activity). Here, we investigated the anti-inflammatory and senomorphic properties of an Asian-native Zingiber officinale Roscoe extract (ZOE), commonly consumed as a food spice and herbal medicine. We employed two models of primary endothelial cells (HUVECs), such as the replicative-senescence and LPS-induced response, to investigate the anti-inflammatory/senomorphic effect of ZOE, and one cellular model of neuroinflammation, i.e., immortalized murine microglial cells (BV2). First, we found that the ZOE treatment induced the inhibition of NF-kB activation in BV2 cells. Among the constituents of ZOE, we showed that the terpenoid-enriched fraction (ZTE) was the component able to counteract the phosphorylation of NF-kB(p65), while 6-gingerol (GIN) and 6-shogaol (SHO) did not produce any significant effect. Further, we observed that the treatment with 10 µg/mL of ZOE exerted anti-inflammatory activity on LPS-stimulated young (y)HUVEC and senomorphyc activity on replicative senescent (s)HUVEC, significantly reducing the expression levels of IL-1β, TNF -α, IL-8, MCP-1, and ICAM-1. Moreover, the ZTE treatment was able to significantly reduce the IL-8 levels secreted in the medium of both LPS-stimulated yHUVEC and sHUVEC. Overall, our data suggest a potential protective role of ZOE on neuroinflammation and endothelial inflammation/activation, thus suggesting its potential relevance in delaying/postponing ARD development and progression, characterized by endothelial dysfunction.

Catalytic Conversion of Beef Tallow to Biofuels using MgO Nanoparticles Green Synthesized by Zingiber officinale Roscoe Rhizome Extract

MgO nanoparticles (MgONPs) have been successfully synthesized using ZOE (Zingiber officinale Roscoe extract in water) and applied for catalytic conversion of beef tallow to bio gasoline, kerosene and diesel. ZOE was used due to containing the alka

Comparative anticonvulsant studies on ethanol and ethyl acetate extracts of Zingiber Officinale Roscoe rhizome in mice and chicks

Epilepsy represents a major public health problem in low- and middle-income countries of Africa and Latin America where over 75% of patients do not have access to treatment. Aqueous and ethanol extracts of Zingiber officinale have been reported previously to have anticonvulsant effect. This study was aimed at comparing the anticonvulsant effect of ethanol and ethylacetate extracts of Zingiber officinale rhizomes in mice and chicks. Fresh rhizomes of Zingiber officinale (ginger) were collected, size reduced, air-dried under shade and extracted with ethanol and ethylacetate using cold maceration. Anticonvulsant activity of the extracts was determined by maximal electroshock stimulation (MES) in chicks, pentylenetetrazole (PTZ) and strychnine induced seizure models in mice. The ethanol and ethylacetate extracts of the ginger at doses of 50, 100 and 200 mg/kg were administered intraperitoneally 30 minutes prior to the induction of seizures. Both ethanol and ethylacetate extracts of Zingiber officinale at the doses of 100 and 200 mg/kg produced a dose-dependent protection against tonic hind limb extension seizure in MES model, significantly (p< 0.01) decreased the onset of clonic seizure in the strychnine model and significantly (p<0.001) prolonged the mean time of death in the PTZ model. In addition, the ethanol extract at the doses of 100 and 200 mg/kg significantly (p<0.001) decreased the onset of seizure in the PTZ model. It might be concluded that both ethanol and ethylacetate extracts of Zingiber officinale contain biologically active compounds with significant anticonvulsant activity, but the ethanol extract produced a better anticonvulsant activity.

UPLC-HRMS Polyphenolic Characterization, Contents and Antioxidant Activity of Zingiber officinale Roscoe rhizomes from Costa Rica

Zingiber officinale Roscoe rhizomes have been associated with multiple health benefits, such as blocking blood clotting, digestive and antinausea effects and aid in respiratory conditions. These effects have been linked with their polyphenolic main metabolites, gingerols and shogaols. Herewith, we report a detailed study on the polyphenolic profile and in the contents of main gingerols and shogaol as well as the antioxidant activity of extracts from Z. officinale rhizomes (n = 17) produced in Costa Rica. Using UPLC-QTOF-ESI MS, a total of 34 polyphenols were identified, grouped in 12 types of structures. In addition, our findings on the main metabolites using UPLC-DAD show all rhizomes complying with total gingerols (TG) content established by the United States Pharmacopeia (USP). At an individual level, samples SR-1 and NR-4 show the higher contents and also exhibit the highest Folin–Ciocalteu (FC) reducing capacity results as well as the best DPPH antioxidant values. In addition, Pearson correlation analysis results showed positive correlation (p < 0.05) between TG and 6-gingerol with FC results and negative correlation (p < 0.05) between 6-gingerol, TG and FC with DPPH results. In turn, Principal Component Analysis (PCA) indicated variability in the composition associated with their region of origin and confirmed that NR-3, NR-4, and especially SR-1 stand out significatively, showing the highest PC1 because of its particularly high TG, 6-gingerol and antioxidant activities. Finally, results from controlled experimental plots of Z. officinale rhizomes (n = 6) indicated influence on metabolites content with higher values for a shorter harvest time and high tunnel cultivation. Therefore, our findings indicate the value of Z. officinale in the elaboration of products with potential benefits for health, delivering extracts with higher levels of gingerols than previous reports and exhibiting high antioxidant activity.

Nanoparticle Production of Zingiber officinale Roscoe Rhizome Extracts by ESS (Expansion of Supercritical Solution)

Aims: To propose a modified RESS method of herbal pharmaceutical extracts nanoparticle production. Background: A vast number of methods have been applied to water-insoluble pharmaceuticals to improve their solubility. Nanoparticle production of pharmaceuticals is considered as one of the high-speed ways to improve solubility. Objective: Supercritical CO2 was applied to extract Zingiber officinale Roscoe rhizome pharmaceutical. Then, a modified RESS (Rapid Expansion of Supercritical Solution) method, called ESS (Expansion of Supercritical Solution), was exerted to obtain NPs (nanoparticles) of the extracted pharmaceuticals. Methods: Initially, applying high pressure in supercritical CO2 contributed to the extract dissolution such that supercritical CO2 was saturated with the sample. Then, by decreasing the pressure, an expansion occurred in the saturated medium. This expansion reduced the power of supercritical CO2 solvent and induced the sample nanoparticle nucleation in the needle valve. Results: LC-MS (Liquid Chromatography-Mass Spectrometry) and EDX (energy dispersive X-ray spectroscopy) result provided solid evidence for the presence of anti-cancer pharmaceutical, [6]-Gingerol, in the extract. The medium size of the nanoparticles in FESEM (Field Emission Scanning Electron Microscopy) analysis was 36 nm. The most satisfactory parameters for a 2 mg mL-1 feeding solution were the initial pressure of 350 atm, secondary pressure of 160 atm, equilibrating time of 10 min, precipitating time of 20 min, and temperature of 48 °C. Conclusion: Unlike rapid expansion of supercritical solution methodology, in this technique, the initial and secondary pressures were permanently above the critical pressure to provide a gentle expansion, which contributes to the production of uniform and small particles. The obtained uniform NPs had a narrow size distribution. Consequently, ESS technique can be considered as an efficient technique for improving the solubility of hydrophobic pharmaceuticals, such as [6]-gingerol.

Quality Assessment of Zingiber officinale Roscoe Essential Oil from Nepal

Zingiber officinale Roscoe rhizome plays a vital role in food flavoring as well as utilization in folk medicine. Nepal is categorized among the leading countries of Z. officinale rhizome production and export. Mature Z. officinale rhizome collection is based on the major production sites within Nepal, but we are currently unaware of the chemical composition of essential oil for export. A comparative investigation of Z. officinale rhizome essential oil compositions, collected from 12 different sites of the eastern, mid, and western regions of Nepal, has been carried out. The analysis of essential oils chemical composition by gas chromatographic–mass spectral (GC-MS), enantiomeric composition by chiral gas chromatography–mass spectrometry (CGC-MS), and antimicrobial activity was evaluated. Essential oil yields ranged from 0.28% to 0.34%. The results showed that the essential oils obtained in this study contained α-zingiberene (8.6%-24.1%), camphene (7.2%-12.8%), β-phellandrene (3.8%-10.1%), neral (0.6%-11.8%), geranial (1.0%-17.4%), ar-curcumene (3.0%-10.3%), and β-sesquiphellandrene (3.7%-9.7%). With CGC-MS, the enantiomeric distributions of 21 chiral compounds were determined and showed no contrasting enantiomeric distributions. Two essential oil samples (G3 and G5) possessed good antibacterial activity against Pseudomonas aeruginosa (MIC = 78.1μg/mL) and excellent antifungal activity against Aspergillus niger (MIC = 39.1 μg/mL). Based on the content of α-zingiberene and citral (neral and geranial), samples collected from their respective areas might be used to identify an ideal Z. officinale rhizome production zone in Nepal.

Phytochemical screening and evaluation of the antioxidant and antibacterial potential of Zingiber officinale extracts

Zingiber officinale roscoe rhizome has been very popular worldwide as a spice, but an auxiliary medicine in treatment of various disorders. In this study, we controlled the effect of the heating extraction in the case of ethyl acetate, ethanol and aqueous extracts on the antioxidant and antibacterial properties. Compounds of ethyl acetate extract were identified by gas chromatography-mass spectrometry (GC-MS). Results showed that zingiberene was present as the major compound (23.71%; 31.70%) followed by β-sesquiphellandrene (15.08%; 16.64%) and curcumene (16.47%; 14.42%) in the ethyl acetate extracts at 20°C and 40°C, respectively. The mineral composition of ginger rhizomes was carried out by the inductively coupled plasma–atomic emission spectroscopy (ICP–AES) analysis. Moreover, ethanol extracts presented the highest polyphenol content (297.63 and 322.11 µg EAG/mg Ex) at 20°C (EET1) and 40°C (EET2), respectively. The two ethanolic extracts were the most active against the DPPH free radicals with IC50 of 98.62 and 77.23 µg/mL, respectively. The two ethyl acetate extracts EAC1 and EAC2 revealed the best antibacterial efficiency compared to the other extracts against all bacterial strains with MIC values ranging from 0.03 to 0.12 mg/mL and MBC values ranging from 0.06 to 0.24 mg/mL. Results showed a good agreement between the antioxidant power of extracts and their content of phenolic compounds.

Philippine Medicinal Plants with Potential Immunomodulatory and Anti-SARS-CoV-2 Activities

Coronavirus disease 2019 (COVID-19) continues to devastate the world’s health and economy, affecting all aspects of life leading to widespread social disruption. Even as several vaccines have been developed, their availability in developing countries is limited and their efficacy against the variants of SARS-CoV-2 (severe acute respiratory syndrome–coronavirus 2) needs to be continuously assessed. The World Health Organization (WHO) has acknowledged that vaccines alone will not overcome the global challenges of COVID-19. Medicinal plants may provide the needed support. Herein, we identify Philippine medicinal plants that possess phytochemicals with potential anti-SARS-CoV-2 activity and/or immunomodulatory properties that may strengthen one’s immune system against COVID-19. These plants were selected from 100 of the best-studied Philippine medicinal plants with antiviral and immunomodulatory properties. The general antiviral and specific anti-SARS-CoV-2 activities and immunomodulatory properties of the phytochemicals that these plants contained were searched. While many compounds assessed individually using in vitro and in silico techniques suggest potential anti-SARS-CoV-2 or immunomodulatory effects, this review sought to identify the medicinal plants which contain these compounds and which, based on literature, have the best potential application against COVID-19. These plants are Allium spp. bulbs (bawang), Andrographis paniculata (Burm.f.) Nees leaves (sinta), Cocos nucifera L. oil (niyog), Euphorbia hirta L. leaves (tawa-tawa), Euphorbia neriifolia L. leaves (sorosoro), Moringa oleifera Lam. leaves (malunggay), Ocimum basilicum L. leaves (balanoy), Piper nigrum L. seeds (paminta), Vitex negundo L. leaves (lagundi), and Zingiber officinale Roscoe rhizome (luya). This review provides a shortlist that can guide research on possible solutions to COVID-19 using Philippine medicinal plants.

Interaction of herbal ingredients contained in the Trikatuk recipe: design of experiment (DOE) and chemical analysis point of view

Trikatuk is an herbal recipe used in Thai traditional medicines since ancient times. However, there is no scientific support about the use of the Trikatuk with an equal weight ratio of its herbal mixture. This work sought to apply the design of experiment to determine the interaction between Zingiber officinale Roscoe rhizomes, Piper nigrum L. fruits, and Piper retrofractum Vahl. infructescences, which are found in the traditional Trikatuk recipe. The chemical markers 6-gingerol and piperine were analyzed using high-performance liquid chromatography. A response surface analysis was also conducted to describe the level of interaction among the three herbal plants. The analysis method was a linear response, specific, precise, and accurate. Estimation of interaction generated using computer software were both precise and reliable. The high extraction yield was achieved at a high Piper retrofractum ratio. The high 6-gingerol content was found at the high Zingiber officinale ratio. The high piperine content was found at the equal ratio of Piper nigrum and Piper retrofractum. According to the interaction, the traditional Trikatuk recipe exhibited at least 200% increase in active compounds yield as compared with the effects of any of the plants individually. Data from this work could be used to describe the interaction of Trikatuk extract. Moreover, they may support that the use of the Trikatuk with an equal weight ratio distribution of the three herbal plants is in fact already appropriate.

Effect of Zingiber officinale Roscoe rhizome (ginger) capsule on postpartum pain: Double-blind randomized clinical trial.

Background:Postpartum pain contributes to increased irritability and excessive stress in the mother and consequently may inhibit successful breastfeeding, reduce a mother's ability to take care of her baby, and cause an imperfect mother-baby interaction. Evidence suggests the positive effect of ginger on reduction in uterus-associated pain. The objective of this study is to investigate the effect of ginger capsules on postpartum pain.Materials and Methods:The present double-blinded, randomized, placebo-controlled trial was conducted in Mahdiyeh Educational Hospital, Tehran. One hundred and twenty-eight mothers having moderate-to-severe pain following vaginal delivery were included. The participants were divided into two groups (A and B). Interventions were performed every 8 h in 24 h. In the first intervention (2 h after the delivery), Group A received 500 mg of placebo capsules (containing chickpea flour) and Group B received 500 mg of Zintoma (ginger rhizome) capsules. In the second and third interventions, Group A received 250 mg placebo capsules and Group B received 250 mg Zintoma capsules. All participants received 250 mg capsules of mefenamic acid in each intervention in addition to ginger or placebo capsules. The pain severity was measured before and half an hour, an hour, and 2 h after each intervention. Statistical analysis was performed using the SPSS software version. 22. The Chi-square, Fisher's, and t tests and the GEE model were applied to assess the pain severity.Results:The average pain severity was not statistically significant between the groups in the beginning of the intervention (P = 0.623). The mean score of pain significantly decreased within the duration of intervention in both groups (P < 0.001); however, the pain severity was significantly lower in the intervention group as compared to the control group at any point after the intervention (P = 0.006).Conclusion:Ginger can be used as an effective remedy for postpartum pain relief.

Extract of zingiber officinale roscoe rhizomes for pharmacological screening anxiolytic activity in rats

This project work study was carried out the pharmacological screening of anxiolytic activity of methanolic extract of Zingiber officinale (ginger) rhizomes in the rat. Ginger is an antique therapeutic plant fitting to Zingiberoside family that is formally called as Zingiber officinale Rosc. The behavioral test was measuring by using the two anxiolytics represent actionlike raised plus maze (EPM) and light-dark arena model. The extraction of ginger is approved out by Soxhlet apparatus utilizing methanol as solvent. The ginger extract dose was administered in two groups as 200 mg/kg, p.o. which is compared with control (received vehicle) and standard groups (diazepam 1 mg/kg, i.p.) for its anxiolytic action for 7 days. The dose at 200 mg /kg and 400 mg/kg administered verbally which increase the time spent and no of entry in raised plus maze and growth time consumed inbox and no of crossing between light dark compartment boxes. The consequences of the training work exposed by extracts exhibit anxiolytic activity. Those female-to-male proportion to At whatever lifetime tension issue may be 3:2. The majority uneasiness issue start over childhood, youthfulness What's more initial adulthood. Frenzy issue exhibits a bimodal period about onset in the age gatherings for 15-24 a considerable length of time. Furthermore, 45- 54 a considerable length of time.

Aqueous Extract of Zingiber officinale Roscoe Rhizomes Sold in Ouagadougou Markets: Phytochemical Constituents, Effect on Arterial Pressure and Blood Biochemical Parameters of Wistar Rats

The aim of this study was to exhibit cardiovascular effects of aqueous extract of Zingiber officinale rhizome sold in Ouagadougou markets (Burkina Faso). Phytochemical constituents and some blood biochemical parameters were also investigated. Colorimetric method was used for phytochemical screening. Extract was prepared and orally administered on Wistar rats. Arterial pressure and cardiac rhythm were measured using Ugo Basile Blood Pressure Recorder 58500. Biochemical parameters were performed using Mindray BA-88A, Semi-auto Chemistry Analyzer. Triterpenes and/or sterols, and saponosides were present in aqueous extract of the plant. We also found very significative hypotension effect on rat mean arterial pressure, when plant extract was administered at 400 mg/kg bw (p ˂ 0.01). When extract was administered after a hypertension induced by L-NAME, we observed a high significative antihypertensive effect (p ˂ 0.001) at 200 and 400 mg/kg bw. This effect was comparable to captopril inhibition of L-NAME induced hypertension. However, in all cases, we did not observe any significative variation of heart rate. For biochemical parameters, we did not find any effect, excepted a significant increase of phosphoremia at 400 mg/kg bw and after L-NAME administration (highly significant). Our results confirm literature data and corroborate traditional uses of Z. officinale sold in Ouagadougou markets. They suggest that antihypertensive effect of AEZO is mainly supported by vascular physiology components.

Zingiber officinale Roscoe rhizome extract alleviates neuropathic pain by inhibiting neuroinflammation in mice

BackgroundCurrent therapies for neuropathic pain are generally symptomatic and possess several side effects, limiting their prolonged usage. Hypothesis/PurposeThus, it is urgent to develop novel and safe candidates for the management of this chronical condition. For this purpose, we investigated the analgesic effect of a standardized extract from Zingiber officinale Roscoe rhizomes (ZOE) obtained by CO2 supercritical extraction, in a mice model of peripheral neuropathy. We also explored the mechanism of action of ZOE and its main constituents using an in vitro model of neuroinflammation. MethodsPeripheral mono-neuropathy was induced in mice, by spared nerve injury (SNI). The analgesic effect of ZOE after oral administration was assessed by measuring mechanical and thermal allodynia in SNI mice. The mechanism of action of ZOE and its main constituents were investigated using spinal cords samples and in an in vitro model of neuroinflammation by ELISA, western blotting and immunofluorescence techniques. ResultsOral administration of ZOE 200 mg kg−1 ameliorated mechanical and thermal allodynia in SNI mice, with a rapid and a long-lasting effect. ZOE did not alter locomotor activity. In BV2 cells and spinal cord samples, ZOE, 6-gingerol and 6-shogaol reduced pERK levels, whereas ZOE and terpene fraction reduced HDAC1 protein levels, inhibited NF-κB signalling activation and decreased IL-1β, TNF-α and IL-6 release. ZOE and each tested constituent had a positive effect on inflammation-impaired SH-SY5Y cell viability. ConclusionsThe oral administration of ZOE attenuated SNI-induced neuropathic pain symptoms by reducing spinal neuroinflammation, suggesting ZOE as a novel and interesting candidate for the management of neuropathic pain.

Molluscicidal Activity of the Essential Oil of Zingiber officinale Roscoe Rhizomes

This work evaluated the molluscicidal activity of the essential oil of the Zingiber officinale Roscoe rhizomes in with of the Biomphalaria glabrata species. The essential oil was isolated quantitatively by hydrodistillation and the oil was characterized analytically by gas chromatography coupled to mass spectrometry and the LC50 of the oil was calculated from the methods of Reed-Muench & Pizzi, respectively. From this, 18 components were identified, the main ones being A-zingiberene, geranial and nerolidol. The essential oil obtained LC50 of 56.23 mg.L-1 and mortality of the snails in the concentration of 75 mg.L-1 in 72 hours. It was concluded that this essential oil has molluscicidal activity.