Zinc Source Research Articles

Comparison of plant extract-derived nano-zinc particles with different zinc sources and effects of different Zn sources on egg yield and quality traits.

This study aimed to compare the zinc (Zn) nanoparticles obtained from plant extracts with different zinc sources and to reveal the effects of different Zn sources on growth performance, egg quality traits and blood serum parameters of laying quail. Treatment groups as follows: control and 3 and 6mg/kg inorganic, organic, and plant extract-derived nano-Zn particles to quail diet supplementation. Animals were fed on experimental diets for 6 weeks. Different Zn sources supplemented into quail diets did not significantly affect live weights (P> 0.05). On the other hand, different Zn sources increased egg yields (P< 0.05). Plant extract-derived nano-Zn supplementations into quail diets increased feed consumption, feed conversion ratio, and egg weights (P< 0.05). However, 6 mg/kg plant extract-derived nano-Zn supplementations reduced egg weights. Different Zn sources did not have any significant effects on egg yolk weight (g) and egg yolk color L*b* values (P> 0.05). For blood serum parameters, 6mg/kg plant extract-derived nano-Zn supplementations increased blood serum AST, ALT, and Zn values (P< 0.05). No adverse effect was observed on the measured values in laying Japanese quails. The findings suggest that plant extract-derived nano-Zn particles could reliably be used in quail feeding.

Effect of feeding various sources of zinc on nutrient digestibility and rumen parameters of male crossbred calves

Effect of feeding various sources of zinc on nutrient digestibility and rumen parameters of male crossbred calves

Food sources of zinc and nutritional status with usual dietary zinc intake in Korean toddlers and preschool children.

This study aimed to evaluate the food sources of zinc and the usual intake of dietary zinc among Korean toddlers and preschool children. A total of 2,679 children aged 1-5 years was selected from the 2009-2013 Korea National Health and Nutrition Examination Survey (KNHANES) data. Dietary data collected from a single 24-h recall were used to evaluate the food sources of zinc. To estimate usual zinc intake, the distribution obtained from single 24-h recall data in the total sample was adjusted using the ratio of within-to-between-person variance in zinc intake obtained from 2-day 24-h recall sub-sample data of the 2009 KNHANES. The proportion of children with usual zinc intake below the estimated average requirement (EAR) and above the tolerable upper intake level (UL) was assessed. The main sources of zinc in Korean children were grains, dairy products, and meat. The mean usual intakes of zinc among all individuals, those aged 1-2 yrs, and those aged 3-5 yrs were 5.50, 5.01, and 5.83 mg/d, respectively. In all participants, 1.1% of the children consumed zinc below the EAR, whereas 10.7% exceeded the UL. The proportion of children with excessive zinc intake was 25.6% in the 1-2 yrs age group and 0.6% in the 3-5 yrs age group. According to the current UL, the risk of excessive zinc intake appears to be high among Korean toddlers. Future studies that monitor the health effects of excessive zinc intake are needed to appropriately guide zinc intake in children.

Dietary zinc intake and sources among Koreans: findings from the Korea National Health and Nutrition Examination Survey 2016-2019.

Zinc is an essential trace mineral which is important for the growth and development of the human body and immunological and neurological functions. Inadequate zinc intake may cause zinc deficiency with its adverse consequences. In this study, we aimed to estimate the dietary zinc intake levels and sources among Koreans. For this secondary analysis, we obtained data from the Korea National Health and Nutrition Examination Survey (KNHANES) 2016-2019. Individuals aged ≥ 1 yr who had completed a 24-h recall were included. The dietary zinc intake of each individual was calculated by applying data from a newly developed zinc content database to the KNHANES raw data. We also compared the extracted data with the sex-, age-specific reference values suggested in the Korean Dietary Reference Intakes 2020. The prevalence of adequate zinc intake was then evaluated by the proportion of the individuals who met the estimated average requirement (EAR). The mean zinc intake of Koreans aged ≥ 1 yr and adults aged ≥ 19 yrs were 10.2 and 10.4 mg/day, equivalent to 147.4% and 140.8% of the EAR, respectively. Approximately 2 in 3 Koreans met the EAR for zinc, but the zinc intake differed slightly among the different age and sex groups. In children aged 1-2 yrs, 2 out of 5 exceeded the upper level of intake, and nearly half of the younger adults (19-29 yrs) and the elders (≥ 75 yrs) did not meet the EAR. The major contributing food groups were grains (38.9%), meats (20.4%), and vegetables (11.1%). The top 5 food contributors to zinc intake were rice, beef, pork, egg, and baechu kimchi, which accounted for half of the dietary intake. The mean zinc intake among Koreans was above the recommended level, but 1 in 3 Koreans had inadequate zinc intake and some children were at risk of excessive zinc intake. Our study included zinc intake from diet only, thus to better understand zinc status, further research to include intake from dietary supplements is needed.

Response of Contrasting Rice Genotypes to Zinc Sources under Saline Conditions

Abiotic stresses are among the major limiting factors for plant growth and crop productivity. Among these, salinity is one of the major risk factors for plant growth and development in arid to semi-arid regions. Cultivation of salt tolerant crop genotypes is one of the imperative approaches to meet the food demand for increasing population. The current experiment was carried out to access the performance of different rice genotypes under salinity stress and Zinc (Zn) sources. Four rice genotypes were grown in a pot experiment and were exposed to salinity stress (7 dS m−1), and Zn (15 mg kg−1 soil) was applied from two sources, ZnSO4 and Zn-EDTA. A control of both salinity and Zn was kept for comparison. Results showed that based on the biomass accumulation and K+/Na+ ratio, KSK-133 and BAS-198 emerged as salt tolerant and salt sensitive, respectively. Similarly, based on the Zn concentration, BAS-2000 was reported as Zn-in-efficient while IR-6 was a Zn-efficient genotype. Our results also revealed that plant growth, relative water content (RWC), physiological attributes including chlorophyll contents, ionic concentrations in straw and grains of all rice genotypes were decreased under salinity stress. However, salt tolerant and Zn-in-efficient rice genotypes showed significantly higher shoot K+ and Zn concentrations under saline conditions. Zinc application significantly alleviates the harmful effects of salinity by improving morpho-physiological attributes and enhancing antioxidant enzyme activities, and the uptake of K and Zn. The beneficial effect of Zn was more pronounced in salt-tolerant and Zn in-efficient rice genotypes as compared with salt-sensitive and Zn-efficient genotypes. In sum, our results confirmed that Zn application increased overall plant’s performance under saline conditions, particularly in Zn in-efficient and tolerant genotypes as compared with salt-sensitive and Zn efficient rice genotypes.

Effects of Zinc Source and Level in Low ABC-4 Diets on Nursery Pig Growth Performance and Fecal Characteristics

Effects of Zinc Source and Level in Low ABC-4 Diets on Nursery Pig Growth Performance and Fecal Characteristics

HEAVY METAL CONTENT IN BEEF COLLECTED FROM LOCAL MARKETS OF DHAKA AND ASSESSING THE HEALTH RISK

Around the world, beef is consumed as a healthy source of protein, fat, niacin, vitamins B6, B12, iron, zinc, and phosphorus. There is mounting evidence that beef is becoming increasingly contaminated with heavy metals, particularly lead (Pb) and chromium (Cr). The purpose of this study was to determine the amounts of the lead (Pb) and chromium (Cr) in beef. Five large markets in Dhaka city—Mirpur-1, New Market, Karwan Bazar, Kaptan Bazar, and town hall market of Mohammadpur—were chosen to gather samples of beef. In this study, the Pb and Cr content in beef was estimated by the Zeeman method using graphite furnace atomic absorption spectrophotometer (GFAAS). The microwave digester finished the digestion of beef. The Pb and Cr content in beef were estimated in mg/kg. Total lead (Pb) content in a beef sample of Mirpur -1, New Market, Karwan Bazar, Kaptan Bazar and Mohammadpur was found to be 0.563±0.084, 0.845±0.127, 0.159±0.024, 0.063±0.009 and 0.143±0.021 mg/kg respectively (Minimum detection limit is 0.06 mg/kg). On the other hand, total chromium (Cr) content in beef sample from Mirpur -1 was found below the detection limit of the method (Minimum detection limit is 0.05 mg/kg). The Cr content in beef from New market, Karwan Bazar, Kaptan bazaar and Mohammadpur was found to be 0.05±0.007, 0.052±0.008, 0.121±0.018 and 0.053±0.008 mg/kg respectively. According to Codex Alimentarium Commission (CAC), the acceptable limit of lead content in beef is 0.1 mg/kg and there is no standard for chromium set by international organization like WHO/FAO, CAC. Brazil has set 0.1 mg/kg as permissible chromium content in beef (Ihedioha et al., 2014). Except for Kaptan Bazar, the chromium content was found to be within Brazil's permissible limit. Surprisingly, only Kaptan Bazar had acceptable lead content that was within the CAC limit. Bioresearch Commu. 9(1): 1196-1202, 2023 (January)

The Threat of Mercury Poisoning by Fish Consumption

(1) Background: Fish is a good source of protein, iron, and zinc, which are key nutrients for infant growth and development, as well as a source of energy for adults and the elderly. Due to mercury contamination in both the terrestrial and aquatic environments, there is a risk that considerable levels of mercury will be ingested by the population, causing undesired illnesses and metabolic symptoms once they reach the body. The purpose of this research was to show and emphasize the hazardous effect of mercury in diverse marine products; (2) Methods: We conducted a comparative, open, randomized, non-interventional study on a study group of 119 adults from varied social and demographic backgrounds to assess the bad repercussions of mercury poisoning from fish consumption. A questionnaire with 18 questions was prepared in order to obtain information about the impact, i.e., the dangers present in fish meat. The research was carried out in Oradea and Bihor County, Romania; (3) Results: The largest age group was between the ages of 25–34, but we had respondents from almost all age groups. Most of them considered the fish healthy (30%) and that the omega-3 fatty acids have beneficial effects on the body (25.10%). More than a half of respondents (55%) were unaware of the hidden danger in fish meat in the form of mercury derivatives. Most respondents (65%) considered that they did not consume enough amounts of fish to suffer the consequences of this phenomenon. More than a half of respondents (59%) agreed that they should have been made aware beforehand by displaying the hazards of excessive intake of some high mercury concentration fish species; (4) Conclusions: It is imperative that the population be informed about dangers of mercury toxicity when consuming fish. Mercury must be gradually eliminated worldwide by replacing it with safer alternatives.

Bamboo and value addition: a boon for industrial development

Bamboo has been increasingly used as a substitute for tropical timber in the International market. Value-added bamboo products are becoming popular due to their characteristics of durability and strength, which resemble the qualities of tropical wood while providing an equally sustainable alternative. Bamboo is considered a grass with rapid growth. As it is a colony plant, it produces new canes (culms) every year from the root system. After 3 to 5 years, culms are ready for harvest, depending on the species. Every year, 5–6 culms can be harvested (from the species that are growing in clumps). Due to this feature, bamboos are mostly used in the rural and semi-urban areas to make houses and so is known as 'poor man's timber. Bamboo shows a very significant part in day-to-day survives (lodging, service, revenue, gasoline, etc.). Lately, bamboo has also found more uses in the manufacturing areas as well as in transitory assemblies. Moreover, being a vital section of cottage and rural business, it is also utilized in some dedicated different usages such bamboo wood, bamboo based-laminates, floor tiling, strengthening in cement material etc. Pulp and paper, Gasoline (charcoal), Fixtures and Handcrafts (picnic basket, jewellery, ball-points, cycles etc.), Building (houses, channels framework, armoured, barriers, concrete, boats), Harmonious Gadgets, Customary drugs, Food (shoots, wine) and silage. Separately from the varied usages of bamboo, the tender sprouts, being little in fat, rich in dietary fibre and mineral content, decent source of potassium, calcium, manganese, zinc, chromium, copper, iron and lesser quantity of phosphorus and selenium. The sprouts are eaten due to their unusual palate, flavour, medicinal and nourishing importance. Diverse value added foodstuffs such as nuggets, pickle and biscuit can be prepared from new bamboo sprouts. Anti-carcinogenic ingredients existing in sprouts marks them a regular portion of regime. It is assumed that bamboo extract offer anti-inflammatory effects. Thus, this paper discusses about the bamboo and its value addition via bamboo-based products industry's future.

Processing of the zinc-lead-bearing flotation middlings by sulfidizing roasting with pyrrhotites production by predicted properties

The accumulated amount of lead-zinc ore flotation tailings in the dumps of concentrating plants today can be considered as independent man-made deposits. In addition to their resource value as sources of lead and zinc, as well as associated gold, silver, cadmium, selenium and other metals, tailings are an environmentally hazardous source of heavy metal pollution of ground and surface waters. The environmental hazard of stale tailings is exacerbated by the fact that they occupy large areas that cannot be used for agricultural or other purposes of the national economy. Wastes of flotation enrichment of lead-zinc ores significantly differ from the source material not only in the content of minerals, but also in the degree of oxidation of their surface, fractional composition, and the presence of a significant amount of mineral intergrowths. In view of this, the use of existing flotation technologies is ineffective for obtaining standard lead and zinc concentrates from enrichment tailings. This paper describes the technology that has been developed for processing of zinc and lead-bearing enrichment wastes by sulfidizing roasting followed by magnetic and flotation concentration of cinders. It was found that, as a result of the sulfidizing- pyrrhotizing roasting process, the flotation ability increases for lead compounds and decreases for iron compounds, while the magnetic susceptibility of lower iron sulfides formed during roasting increases. It has been established that sulfidizing takes place with sufficient completeness, and during subsequent flotation, it is possible to extract up to 95% of zinc and up to 80% of lead into sulfide concentrate. These results have a technological advantage in contrast to the other methods that have been used. It was found that at roasting temperatures of 700&ndash;800 &deg;C, pyrrhotites have a maximum magnetic susceptibility of 3.75, 5.43 and 2.18 SI units for Fe0.855S, Fe0.888S and Fe0.909S, respectively. Technological recommendations are acceptable for similar raw materials. The research was funded by the Science Committee of&nbsp;the Ministry of Education and Science of the Republic of&nbsp;Kazakhstan Grant №AP08052829.

Synthesis, characterization, and 5-fluorouracil release behavior of superparamagnetic γ-Fe2O3/ZnO hetero-nanostructures for biomedical applications

In this study, superparamagnetic maghemite (γ-Fe2O3) nanosystems in the form of nanoparticles were fabricated through the co-precipitation method at different processing conditions. Additionally, novel γ-Fe2O3/ZnO heterostructures were fabricated using zinc acetate as the zinc source and polyvinyl pyrrolidone (PVP), and ethylenediaminetetraacetic acid (EDTA) as the capping agents. The results revealed that the size of the crystallites of the prepared maghemite nanopowders was between 6 nm and 7.8 nm depending on the synthesis temperature. The saturation magnetization value of maghemite nanopowders synthesized at 90 °C was measured to be 51.58 emu/g without hysteresis with negligible coercivity. FTIR analysis results proved the presence of ZnO in the maghemite core-based nanosystem. The formation of a ZnO coating using zinc acetate in the presence of PVP and EDTA as a capping agent caused a decrease in magnetization value to 12 emu/g. A ZnO coating provided better dispersion of maghemite nanoparticles and it also affected the drug (5-FU) delivery behavior. ZnO-coated nanoparticles showed prolonged and sustained drug release additionally, they continue to release the drug after 410 h. The 5-FU release kinetics of the prepared heterogeneous nanostructures fitted well with the Korsmeyer-Peppas model.

Nutritional Composition and Antinutrient to Mineral Molar Ratios of Selected Improved Common Beans Grown in Kenya

A decline in common bean production has been ascribed to climate change. The adoption of improved beans aims to increase productivity, profitability, and consumption, thus reducing food and nutrition insecurity in the country. The aim of this study was to determine the proximate composition, antinutrient content, mineral content, and bioaccessibility of zinc and iron in two improved bean varieties grown in Kenya; Faida (biofortified) and RM 01 (drought tolerant)). The protein content of RM 01 (22.48%) was significantly higher than the Faida bean variety (20.90%). RM 01 bean variety had higher crude fat (4.20%) and crude fiber (4.31%) content compared to Faida which had 3.78% and 3.31% for crude fat and crude fiber respectively. Faida recorded significantly higher levels of iron (61.5 mg/kg) and zinc (26.8 mg/kg) content. Faida beans also had significantly (p&lt; 0.05) high levels of phytates (11.70 mg/g) and tannins (4.39 mg CE/g). Phytate to iron ratio for Faida was 17.08 and RM 01 was 15.19 while the phytate-to-zinc ratio was 42.26 and 35.36 for Faida and RM 01 respectively. The RM 01 bean variety had iron bioaccessibility of 35% and zinc bioaccessibility of 65% compared to the Faida bean variety which had bioaccessibility of 29% and 42% for iron and zinc respectively. In conclusion, RM 01 variety is a better source of iron, zinc, and protein compared to the Faida variety.

Drought Stress in Lentil (Lens culinaris, Medik) and Approaches for Its Management

Lentil plays an important role for food and nutritional security. It is a sustainable source of protein, zinc, iron, prebiotic carbohydrates, and diverse health-promoting nutrients. This crop is widely cultivated in semi-arid marginal areas and exposed to various environmental stressors. Drought stress is the major abiotic stress that causes serious effects on lentil growth and development. Thus, it is imperative to set up innovative and sustainable solutions to reduce the adverse effects of drought on lentil crop. In this review, the agro-morphological, physiological, and biochemical effects of drought on lentil were highlighted. Furthermore, breeding and agronomic interventions to improve lentil performance in drought-prone environments were also discussed. Overall, drought disturbs lentil germination, photosynthesis, water relations, shoot and root growth, thereby reducing final yield. Conventional breeding programs have identified several sources of drought tolerance; however, modern biotechnological tools could be adopted to decipher the genetic architecture of drought tolerance in lentil to accelerate the genetic progress. Cost-affordable and eco-friendly agronomic practices may also contribute to minimize the negative consequences of drought stress. Smart exploitation of breeding approaches and agronomic practices could help overcome drought, improve lentil productivity, and increase the profitability of farmers in dry areas.

Effect of fluoride complexation by aluminium ions on the corrosion behaviour of aluminium cathodes used in zinc electrowinning

Fluoride (F−) impurity at 20 mg L−1 in the zinc sulphate electrolyte increased by 73% and 98% the corrosion rate of Al-1050 and Al-1070 alloys, respectively, which are used as cathode in zinc electrowinning process. The addition of aluminium ions, Al3+, to the electrolyte reduced the corrosion rate by about 21% for the Al-1050 alloy and about 28% for the Al-1070 alloy. The effect was ascribed to the formation of AlF2+ and AlF2+ complexes, thus decreasing fluoride activity. Electrochemical impedance spectroscopy demonstrated that the corrosion resistance of Al alloys is increased in the presence of Al3+. Scanning electron microscopy indicated uniform corrosion of the Al surface, the degree of which depends on fluoride concentration. The higher corrosion resistance of Al-1050 was related to its coarser microstructure, which is less susceptible to corrosion in a non-passivating media. The selection of effective complexing agent for fluoride ion and the consequent reduction in the corrosion rate of aluminum cathodes are relevant in scenario of increasing co-processing of secondary zinc sources.

Theoretical and Practical Evaluation of the Feasibility of Zinc Evaporation from the Bottom Zinc Dross as a Valuable Secondary Material

This study presents a theoretical and practical evaluation of zinc evaporation from bottom zinc dross (hard zinc) as a secondary zinc source (zinc content approximately 94-97%), which originates in the batch hot-dip galvanizing process. The thermodynamics of the zinc evaporation process were studied under the normal pressure (100 kPa) in the inert atmosphere, using argon with flow rate 90 mL/min. Samples were subjected to the evaporation process for 5, 10 and 20 min under the temperature of 700 °C and 800 °C, respectively. For the theoretical thermodynamic study, HSC Chemistry 6.1 software was used and final products, as well as residuals after the evaporation process, were analyzed by SEM (Scanning Electron Microscopy) and EDX (Energy Dispersive X-ray). Calculated and experimental argon consumption in the process of zinc evaporation has been compared. A high purity zinc with efficiency over 99% was reached. Due to a dynamic regime, argon consumption at the temperature of 700 °C and 800 °C were 7 times and 3 times, respectively, less than calculated.

A lean‐zinc anode battery based on metal–organic framework‐derived carbon

AbstractImproving zinc metal (Zn0) reversibility and minimizing the N/P ratio are critical to boosting the energy density of Zn0 batteries. However, in reality, an excess Zn source is usually adopted to offset the irreversible zinc loss and guarantee sufficient zinc cycling, which sacrifices the energy density and leads to poor practicability of Zn0 batteries. To address the above conundrum, here, we report a lean‐Zn and hierarchical anode based on metal–organic framework (MOF)‐derived carbon, where trace Zn0 is pre‐reserved within the anode structure to make up for any irreversible zinc source loss. This allows us to construct low N/P ratio Zn0 full cells when coupling the lean‐Zn anode with Zn‐containing cathodes. Impressively, high Zn0 reversibility (average Coulombic efficiency of 99.4% for 3000 cycles) and long full‐cell lifetime (92% capacity retention after 900 cycles) were realized even under the harsh lean‐Zn condition (N/P ratio: 1.34). The excellent Zn reversibility is attributed to the hierarchy structure that homogenizes zinc ion flux and electric field distribution, as confirmed by theoretical simulations, which therefore stabilizes Zn0 evolution. The lean‐Zn anode design strategy will provide new insights into construction of high‐energy Zn0 batteries for practical applications.

Effect of AgNps and different sources of Zinc on rooting and acclimation of strawberry (Fragaria ananassa Duch) cv. Rubygem in vitro

This study was conducted to examine the effect of adding different concentrations of nano silver nitrate (0, 5, 10 and 15 mg L-1) and zinc source {traditional(0.86mg L-1 as ZnSO4.7H2O) and nano zinc 0.086mg L-1 as Zinc oxide) to the specifies medium of rooting strawberry (Fragaria X ananassa Duch) cv. Rubygem, in addition to testing the efficiency of nano silver nitrate and zinc source on successful percentage of cultures adapting stage. Results revealed that highest rooting percentage, root numbers, roots length, leaves content of total chlorophyll and macro nutrients (N, P and K) were achieved the highest average at 10mg L-1 + nano zinc treatment. The source of cultures resulting from rooting stage affected the plant survival at acclimatization stage. The treatment 10 and 15mg L-1 of nano silver nitrate + nano zinc achieved 100% success percentage.

Nutritional Evaluation of Nano Zinc Compared with Other Zinc Sources in Broilers

Nutritional Evaluation of Nano Zinc Compared with Other Zinc Sources in Broilers

Nano Zinc Oxide Improves Performance, IGF-I mRNA Expression, Meat Quality, and Humeral Immune Response and Alleviates Oxidative Stress and NF-κB Immunohistochemistry of Broiler Chickens

A 35-day trial was set to explore the effects of different dietary zinc sources on growth, insulin-like growth factor I (IGF-I) mRNA expression, meat quality, immune response, antioxidant activity, and immunohistochemistry of nuclear factor kappa B (NF-κ7B) of broiler chickens. Ross 308 broiler chicks (n = 156) were randomly assigned into four experimental groups. The G1 received the basal control diet without zinc supplementation; the G2, G3, and G4 were supplemented with zinc oxide, zinc lysine, and nano zinc oxide, respectively, at a level of 40 mg Zn/kg diet. The data revealed that nano zinc oxide linearly improved the overall growth performance parameters. Nano zinc oxide linearly elevated (P < 0.001) mRNA expression of IGF-I followed by G3. The pH value of breast muscle in G4 shows a linearly decreasing value (P < 0.001). Also, the linearly highest expressible release volume percentage and lightness (L*) value with the lowest redness (a*) value (P < 0.05) were recorded in G4 and G3. A numerical increase in the total antibody titer was recorded on the 35th day in the G3 and G4. A numerical elevation in the superoxide dismutase (SOD) and a numerical reduction in the serum malondialdehyde (MDA) were recorded in the G4. The section of the liver from G4 revealed significantly very low expression of NF-κB staining. It is concluded that nano zinc oxide is considered the more trending zinc source. It had no negative effects on the health status and can be used in broiler diet premix.

Biscuits Prepared with Enzymatically-Processed Soybean Meal Are Rich in Isoflavone Aglycones, Sensorially Well-Accepted and Stable during Storage for Six Months.

Soybean meal (SBM) is a co-product of the soybean oil industry that is rich in bioactive compounds, such as isoflavones. We aimed to study the effects of processing SBM by fermentation (Saccharomyces cerevisiae) (FSBM) and enzymatic hydrolysis (CelluMax C, a commercial cellulase) (ESBM) on its chemical composition, with emphasis on isoflavones. Fermentation increased protein content by 9%, ash content by 7%, dietary fiber by 11% and minerals by up to 38%, except for iron, which decreased by 26%. Fermentation completely removed oligosaccharides from SBM, while enzymatic processing decreased oligosaccharides by 45% in SBM. Both processes converted glycosylated isoflavones into the corresponding aglycones, the content of which increased by up to 7.7-fold. Biscuits containing SBM, FSBM and ESBM could be labeled as dietary sources of dietary fibers, potassium, phosphorous, calcium and zinc, as well as high in proteins, copper, iron, manganese and magnesium. While FSBM biscuits had lower sensory scores compared to SBM biscuits, ESBM biscuits had equivalent scores. During storage for 180 days at room temperature, the isoflavone profile of all biscuits remained stable. Moreover, storage did not impair microbiological and sensory qualities of any biscuits. Altogether, ESBM biscuits show great marketing potential.