The study of twisted representations of graded vertex algebras is important for understanding orbifold models in conformal field theory. In this paper, we consider the general setup of a vertex algebra V, graded by \documentclass[12pt]{minimal}\begin{document}$\Gamma /\mathbb {Z}$\end{document}Γ/Z for some subgroup Γ of \documentclass[12pt]{minimal}\begin{document}$\mathbb {R}$\end{document}R containing \documentclass[12pt]{minimal}\begin{document}$\mathbb {Z}$\end{document}Z, and with a Hamiltonian operator H having real (but not necessarily integer) eigenvalues. We construct the directed system of twisted level p Zhu algebras \documentclass[12pt]{minimal}\begin{document}$\operatorname{Zhu}_{p, \Gamma }(V)$\end{document}Zhup,Γ(V), and we prove the following theorems: For each p, there is a bijection between the irreducible \documentclass[12pt]{minimal}\begin{document}$\operatorname{Zhu}_{p, \Gamma }(V)$\end{document}Zhup,Γ(V)-modules and the irreducible Γ-twisted positive energy V-modules, and V is (Γ, H)-rational if and only if all its Zhu algebras \documentclass[12pt]{minimal}\begin{document}$\operatorname{Zhu}_{p, \Gamma }(V)$\end{document}Zhup,Γ(V) are finite dimensional and semisimple. The main novelty is the removal of the assumption of integer eigenvalues for H. We provide an explicit description of the level p Zhu algebras of a universal enveloping vertex algebra, in particular of the Virasoro vertex algebra \documentclass[12pt]{minimal}\begin{document}$\operatorname{Vir}^c$\end{document}Virc and the universal affine Kac-Moody vertex algebra \documentclass[12pt]{minimal}\begin{document}$V^k(\mathfrak {g})$\end{document}Vk(g) at non-critical level. We also compute the inverse limits of these directed systems of algebras.