NaY Zeolite Research Articles

Study of adsorption and immobilization of Cs+, Sr2+, Co2+, Pb2+, La3+ ions on Na-Faujasite zeolite transformed in solid state matrices

Zeolites have become promising adsorbents for wastewater treatment due to their enhanced adsorption capacity, stability of crystalline structure, high porosity and surface area. One of the primary goals of our study was to assess the effectiveness of employing NaY zeolite as a sorbent and potential solid matrix for immobilizing radionuclides. In this study NaY faujasite zeolite was obtained by hydrothermal synthesis and characterized by X-Ray diffraction (XRD), N2 adsorption–desorption, scanning electron microscopy (SEM) and energy dispersive spectroscopy (EDS) methods. The prepared zeolite had NaY faujasite crystalline structure, characterized by developed surface area (370 m2/g) with micro-mesoporous structure, and spherical-like morphology with particles of 1–4 μm in diameter. The isotherms adsorption modeling on Cs+, Sr2+, Co2+, Pb2+ and La3+ ions was performed. The isotherm well described by Langmuir equation with maximum adsorption capacity of q(Cs+) = 1,9 mmol/g, q(Sr2+) = 3,75 mmol/g, q(Co2+) = 1,82 mmol/g, q(Pb2+) = 2,54 mmol/g, q(La3+) = 3,83 mmol/g. Thermal behavior of metal-saturated adsorbents by differential thermal analysis (DTA) and thermal gravimetric (TG) techniques was studied. Each metal examined in this paper is responsible for the stable formation of radionuclides (137Cs, 90Sr, 60Co, La - generally, the group of active lanthanides was modelled, Pb - uranium fission residues) that are generated during the operation of nuclear power plants. To optimize sintering regimes, it has been proposed to achieve sorption saturation of stable ions such as Cs, Sr, Co, La, and Pb and transfer them into solid matrices. It was shown that the consolidation temperature for the obtained samples varies in the range of 935–1040 °C.

Study on iodine adsorption capacity and adsorption mechanism of AgY

ABSTRACT To systematically study the methyl iodide (CH3I) adsorption performance and adsorption mechanism of Ag ion exchange NaY zeolite (AgY), AgY adsorbents with different Ag content were selected. 16.6% AgY was capable of storing 150 mg/g and 275 mg/g of CH3I when the penetrated CH3I reached 1‰ and 100% inlet concentration. TEM and EDS results presented a high relationship between Ag dispersion and CH3I adsorption capability. The formation of a stable AgI complex rationalized the CH3I adsorption mechanism on AgY. Compared to AgX, AgY was verified to possess a strong acid resistance by FT-IR, SEM and XRD. The effect of three key parameters (temperature, humidity and gas velocity) on CH3I adsorption was carefully investigated by orthogonal experiment design. The experimental results showed that AgY had better iodine removal performance at a high temperature, low humidity, and low flow rate. This work provides technical reference for further engineering applications of 16.6%AgY in spent fuel reprocessing plants.

Formic acid as a sacrificial agent for byproduct suppression in glucose dehydration to 5-hydroxymethylfurfural using NaY zeolite catalyst

Biomass-derived 5-hydroxymethylfurfural (HMF) holds potential for applications in green materials, but its conventional synthesis is hindered by undesired side reactions. This study presents a catalytic system that effectively suppresses the formation of byproducts, thus enhancing HMF yield. The system demonstrated synergistic effects between Lewis acid NaY zeolite and formic acid sacrificial agent for the production of HMF from glucose. The results indicate that formic acid reacts with reactive intermediates from glucose decomposition, preventing their interactions with other sugar-derived species in the dehydration path to HMF. Such effect originates from the neutral formic acid species rather than the dissociated acidic proton normally observed in Brønsted acid-catalyzed reactions. The NaY/formic acid catalysts in isopropanol/water achieved a 57% HMF yield, a significant improvement over 31% and 27% yields with NaY or formic acid alone, respectively. Moreover, performance of the spent catalysts was easily restored to the original state via a simple NaCl wash.

Facile synthesis of regenerable NaY zeolite adsorbent relying on alcohol-terminated compounds for efficient trace sulfur dioxide capture

The selective removal of sulfur dioxide (SO2) is of importance for the desulfurization and purification of exhaust gases, but it is still challenging to design adsorbents with high capture capacities (particularly at low partial pressures) and great cyclic stability. Herein, a facile strategy is proposed to optimize the adsorption performance of NaY zeolites, where various alcohol-terminated compounds (n-pentanol, ethylene glycol, polyethylene glycol) with different molecular structures are introduced as auxiliaries to facilitate the zeolite nucleation process and the formation of microporous structures. These alcohols promote the crystallization of NaY, increase the specific surface area and expand the microporosity due to the formation of micelles during synthesis. Coupled with the rise of surface basic sites, the chemisorption and physisorption of SO2 on designed zeolites is considerably improved. The PEG-Y sample derived from polyethylene glycol gives the highest dynamic adsorption of SO2 (315 mg/g), high-level SO2 equilibrium adsorption (2.18 mmol/g) under exceptionally low pressure (0.002 bar), preferred separation performance of SO2 from SO2/N2, robust stability and great regenerability. This technique can be further employed to fabricate analogous adsorbents for other gas adsorption and separation processes.

Copper-carboxamide complex immobilized on nano NaY zeolite: an efficient catalyst for xanthenes synthesis

Copper-carboxamide complex immobilized on nano NaY zeolite: an efficient catalyst for xanthenes synthesis

Active and passive corrosion protection coating based on NaY zeolite

The coating of a surface with organic material is the simplest and most effective method to prevent metal materials from corrosion. However, pure resin coatings are prone to corrosion, as corrodents can penetrate the metal surface via micropores. Moreover, passive corrosion resistance, achieved by adding a filler to a coating to lengthen the penetration path of the corrodent, is not sufficient to prevent corrosion. Therefore, the development of coatings with active and passive corrosion resistance properties is necessary to extend the lifetime and enhance the performance of coatings. Herein, a coating with active and passive corrosion resistance properties was designed by loading a NaY zeolite nanocarrier with the corrosion inhibitor benzotriazole (BTA), with a NaY zeolite nanocarrier loading capacity of 7.22 %. The release properties of the resulting NaY@BTA and the corrosion inhibition of BTA released under different pH conditions were investigated. The adhesion strength, contact angles (CAs), and water absorption of the composite coatings were evaluated. The coating with 0.5 % nanocarrier exhibited an adhesion strength of 4.68 MPa, a CA of 84.78°, and the lowest absorbed water content of 1.28 %. The corrosion resistance of composite coatings with different amounts of NaY@BTA was compared, with the electrochemical impedance spectroscopy data of intact and scratched coatings displaying their excellent active corrosion resistance and self-repair properties. After immersion in 3.5 % NaCl solution for 60 days, the low frequency Z0.01 Hz value of EP–PU-0.5%NaY@BTA was 1011 Ω.

Polyacrylonitrile (PAN)/Freeze-dried Chitosan (FCS)-NaY zeolite triple layer nanostructure fibrous adsorptive membrane (PAN/FCS-NaY TNAM) for Cu(II) and Pb(II) ions removal from aqueous solutions

In this study, an innovative PAN/FCS-NaY TNAM was fabricated using an electrospinning technique for Cu(II) and Pb(II) removal from contaminated water. Top and bottom fibrous layers (M1) were fabricated using a combination of FCS and PAN blends. Middle layer (M2) was electrospun from pure PAN solution and loaded with NaY zeolite particles. The physicochemical properties of the material and fibrous samples were investigated through SEM, FTIR-ATR, DSC, AFM, DMTA analysis, and contact angle measurement. The effective parameters, including initial ions concentration, time, reusability, and pH on the dynamic adsorption performance of the prepared membrane and its stability in the aqueous medium for Cu(II) and Pb(II) ions removal were investigated at a pressure of 0.006 bar. Dynamic metal ions adsorption was modeled using dynamic filtration prediction models such as Thomas and Yoon-Nelson. The fabricated nanostructure fibrous membranes with the removal percentage of Cu(II) and Pb(II) ions (94.6% and 98.7%, respectively) under the optimal operating conditions (initial concentration: 50 ppm, time: 10 min, and pH: 4.6), high compatibility in modeling dynamic filtration (R2 > 0.99), high pure water flux (298 LMH), suitable reusability (four-cycle without significant reduction in adsorption) as well as long-term stability, can be as potential filters in water treatment applications.

From Basic Research on Supported-Metal Catalysts to their Practical Applications for Reduction of Food Waste

Abstract Supported metal catalysts are one of the most important heterogeneous catalysts, and they are widely used in chemical processes such as naphtha reforming, purification of exhaust gas from automobiles and so on. However, rational design of supported metal catalysts is still a target in heterogeneous catalysis. The author had a hypothesis that metal nanoparticles in ordered pores of zeolite or mesoporous silica may exhibit novel catalytic properties. Based on this idea, we used micropores of zeolites to accommodate metal carbonyl clusters, and then mesoporous silica to prepare metal nanoparticles. First, we studied ship-in-bottle synthesis of Rh carbonyl clusters in zeolite and its reactivity. Rh6(CO)16 was synthesized in NaY zeolite accompanied with formation of a mononuclear Rh(CO)2, which acted as a carrier of 13CO in 12CO-13CO exchange reaction. Then we worked on synthesis of platinum nanoparticles in mesoporous silica, and found that high activity in preferential oxidation of CO in hydrogen and in low-temperature oxidation of ethylene. The high catalytic performance in ethylene oxidation has led to practical application of platinum/silica catalysts for preservation of fruits and vegetables, resulting in reduction of food waste. This article describes the development process of this research.

Separation of the Chlorofluorocarbon (CFC) CCl2F2 from N2 in NaY Zeolite, in MIL-127(Fe) and in the two Carbon Nanotubes CNT (9,9) and CNT (11,11)

Four well-suited porous materials for the selective adsorption of the most prominent CFC, which is CCl2F2, from the air are carbon nanotubes CNT (9,9) and CNT (11,11), NaY zeolite, and the Metal Organic Framework MIL-125(Fe). The adsorption has been investigated through molecular simulations. Simulation results and theoretical considerations show that reasons for the extraordinarily high selectivity in all four cases were found to be the differences in the enthalpy of adsorption for the various adsorbed gases rather than steric reasons. The four adsorbate–adsorbent systems have been examined at different temperatures, pressures, and concentration ratios in the mixture. Among them, the carbon nanotube CNT (11,11) exhibited the highest selectivity, reaching up to 104.

Effect of Hydrogen Adsorption on Pt Nanoparticle Encapsulated in NaY Zeolite: Combined Study of WT XAFS and DFT Calculation

Extensive research has been conducted on platinum nanoparticles or clusters supported on zeolite for various catalytic applications, primarily due to the well-defined structure contained within the pore. The preparation and characterization of these particles have been thoroughly examined using advanced techniques such as X-ray absorption fine structures (XAFSs), both in situ and ex situ. In this study, we employed the Wavelet method to analyze the structure of platinum nanoparticles encapsulated within the supercage of a Y zeolite, where XAFS data were collected over a temperature range of 100 K to 423 K, both with and without hydrogen. The adsorption of hydrogen caused a relaxation in the structure of the platinum nanoparticles, thus leading to a decrease in the Pt–Pt distance and resulting in a lower Debye–Waller factor compared to bare nanoparticles. This structural change induced by hydrogen chemisorption aligns with the findings of the density functional theory (DFT) calculations for Pt13 nanoparticles located in the supercage. The relaxation of the structure results in charge redistribution, thereby ultimately generating atomic hydrogen with a partial negative charge, which is crucial for catalytic processes.

Sustainable fluorescent dye-faujasite zeolite systems as tools for cancer bioimaging

The use of fluorescent nanoparticles for biological and medical applications has attracted great attention due to their several advantages compared to conventional fluorescent dyes. However, there is also a need for sustainable and versatile alternatives, alongside the streamlining of complex synthesis or preparations. In this work, we study the potential of the faujasite zeolite structure to encapsulate dye molecules and be applied as a bioimaging agent for cancer cells. The commercial zeolites NaY, USY, and NaX were used to encapsulate Rhodamine B (RhB) or Fluorescein (F) dyes, which present a similar tetracycle core structure. Different characterization techniques confirmed the encapsulation of the dyes and their stability. The photophysical characterization of the encapsulated RhB and F dyes was also elucidated by UV–visible and fluorescence spectroscopy. Biocompatibility was tested in different cancer cell lines and confocal laser scanning electron microscopy (CLSM) was used to track the fluorescent dye-containing zeolites. Remarkably, the dye-containing zeolite RhB@USY showed interesting multiluminescent labeling properties that make it a suitable agent for bioimaging applications. The economic feasibility of the developed fluorescent dye-zeolite probes was also examined through a detailed cost analysis, which further highlights their commercial potential.

Decorating {0 0 1} TiO2 nanosheets on hydrophobic NaY zeolite: An efficient deactivation-resistant photocatalyst for gaseous toluene removal

Photocatalysis is regarded as a promising in situ air purification technology for the removal of volatile organic compounds. However, the deactivation of photocatalysts remains a serious issue that needs to be addressed. Herein, we report a novel dual-functional photocatalyst (TiO2@HYZ) with spatially separated catalytic sites and adsorption sites, which has been obtained by combining hydrophilic {001} TiO2 nanosheets with hydrophobic NaY zeolite. TiO2@HYZ showed excellent photocatalytic activity for the degradation of gaseous toluene under UV irradiation in humid air. The optimized TiO2@HYZ-2 (containing 45.7 wt% TiO2) achieved 96.6% toluene removal and 87.4% mineralization within 120 min. The specific reaction rate constant of TiO2@HYZ-2, pristine TiO2 and P25 normalized by the unit amount of TiO2 during the photocatalytic degradation process were calculated to be 0.481, 0.230 and 0.250 min−1 g−1, respectively. Moreover, TiO2@HYZ-2 showed superior durability, maintaining stable performance over repeated toluene degradation processes, even at high toluene concentration, whereas pristine TiO2 incurred severe deactivation with increasing number of photocatalysis cycles. The significantly enhanced activity and anti-deactivation property was attributed to a synergistic effect between HYZ and TiO2 in the catalyst structure, which strengthened the adsorption of toluene on HYZ and facilitated effective separation of photoexcited charge carriers on TiO2, thus avoiding the deposition of degradation intermediates on the active sites and simultaneously improving mineralization. This work provided a new perspective for the design of efficient and deactivation-resistant photocatalysts for air pollution remediation.

Synthesis and Characterization of Zeolite NaY Dispersed on Bamboo Wood.

Zeolites in powder form have the potential to agglomerate, lowering access to active sites. Furthermore, a suspension of fine zeolite powder in liquid media is difficult to separate. Such drawbacks could be improved by dispersing zeolite crystals on support materials. This work demonstrates the dispersion of zeolite NaY crystals on bamboo wood by mixing the wood with zeolite gel before hydrothermal treatment. The syntheses were performed with acid-refluxed and non-refluxed wood. The phase of zeolites, particle distribution and morphology, zeolite content in the wood, and zeolite-wood interaction were investigated using X-ray diffraction, X-ray tomography, scanning electron microscopy, thermogravimetric analysis, nitrogen sorption analysis, and X-ray photoelectron spectroscopy. Higher zeolite content and better particle dispersion were obtained in the synthesis with the acid-refluxed wood. The composite of NaY on the acid-refluxed wood was demonstrated to be an effective adsorbent for Ni(II) ions in aqueous solutions, providing a higher adsorbed amount of Ni(II) per weight of NaY.

Synthesis of magnetic NaY zeolite for plasma proteomics application

Magnetically modified NaY zeolite has shown advantages in high-throughput and large-scale clinical plasma proteomic studies. In this study, magnetic NaY zeolite was prepared via an improved method, "layer by layer adhesion", using epoxy adhesive. The morphology, structure, and magnetic properties of magnetic NaY zeolite were characterized and tested by scanning electron microscope (SEM), fourier transform infrared spectrometer (FT-IR), X-ray diffractometer (XRD), and vibrating sample magnetometer (VSM), and using sodium dodecyl sulfonate-polyacrylamide gelelectrophoresis (SDS-PAGE) and liquid chromatography-tandem mass spectrometry (LC-MS) for plasma proteomics studies. The results showed that NaY zeolite was coated on the surface of Fe3O4 by epoxy adhesive, and such coating did not affect the surface properties of the magnetic zeolite. The saturation magnetization of magnetic NaY zeolite was measured as 9.964 emu g−1. Approximately 2929 proteins could be identified from plasma protein corona formed on magnetic NaY zeolite, reaching 97.6 % of the identification number of pure NaY zeolite. Furthermore, compared with NaY zeolite, the solid-liquid separation processes of magnetic zeolite during plasma proteomics studies were significantly reduced from half an hour to few minutes. Due to excellent plasma protein adsorption performance and rapid magnetic separation property, magnetic NaY zeolite is expected to become a powerful tool for the large-scale adsorption of low-abundant plasma proteins and high-throughput proteomic studies.

Examining the impact of different anions in Cu precursors on sulfur adsorption through zeolites with Cu ion-exchange

The majority of hydrogen is generated by converting hydrocarbons, with natural gas reforming accounting for over 50% of the total hydrogen production. However, the utilization of natural gas in generating hydrogen causes catalyst deactivation during the reforming process because of presence of sulfur compounds as odorants in commercial natural gas. Therefore, the removal of sulfur compounds from natural gas should be prioritized before its application in hydrogen production. Adsorption using Cu-ion-exchanged zeolites is an effective method for removing sulfur compounds from natural gas. In this study, the adsorptive desulfurization performance of tetrahydrothiophene (THT) was evaluated using pristine NaY and Cu-ion-exchanged Y zeolites. In particular, the effects of anions in the Cu compounds used for the ion exchange of zeolites were investigated using three Cu precursors (Cu(NO3)2, Cu(Ac)2, and CuSO4). Among the tested zeolite adsorbents, the NaY zeolite ion-exchanged with Cu(Ac)2 exhibited the highest adsorption uptake, whereas the NaY zeolite ion-exchanged with CuSO4 demonstrated the lowest adsorption uptake for THT. The analytical results indicated that following the exchange of Cu ions, a rearrangement of Si and Al was observed within the zeolite framework, resulting in the Si site distribution. The rearrangement of the framework and distribution of Si sites was influenced by the anions present in the Cu precursors. In addition, it was observed that Cu2+ primarily occupied two locations within the zeolite, namely the supercage and the sodalite cage, and the distribution of Cu2+ between these two sites varied amongst the Cu-ion-exchanged zeolites. This discrepancy was linked to the adsorption uptake for THT.

Ugi‐modified nano NaY zeolite for the synthesis of new 1,5‐dihydro‐2H‐pyrrol‐2‐ones under mild conditions

In this study, it was aimed to modify the nano zeolite surface via a multi‐component reaction to obtain high functionality in one pot process. For this purpose, the Ugi‐reaction was applied for the first time to develop a novel hydrogen‐bonding nanocatalyst. The nano NaY zeolite was post‐modified using 3‐aminopropyltriethoxysilane (APTES), followed by the Ugi reaction with salicylaldehyde, tert‐butyl isocyanide, and acetic acid to create carboxamide‐functionalized nano NaY. The presence of hydroxyl groups, pores, and carboxamide moieties on the surface of nano zeolite could improve the reactivity, dispersibility, and hydrogen bonding formation capability of the nano zeolite. The formation of the nanocatalyst was investigated by Dynamic Light Scattering (DLS), X‐Ray Diffraction (XRD), Differential Thermal Analysis (DTA), Fourier Transform Infrared spectroscopy (FT‐IR), Thermogravimetric Analysis (TGA), Transmission Electron Microscopy (TEM), Brunauer–Emmett–Teller (BET), Scanning Electron Micrograph (SEM), Elemental analyses, and Energy Dispersive Spectroscopy (EDS). The catalytic activity of the synthesized modified nano zeolite was examined in the three component‐reaction of aldehydes, amines, and ethyl pyruvate to obtain new pyrrol derivatives in high yields under mild conditions. After separation by filtration, the catalyst can be recycled for up to seven subsequent runs without any appreciable activity loss. Thus, the titled heterocycles can be prepared in an eco‐friendly manner since the catalyst is easily recovered and reused, and the procedure is simple to perform. The results indicated that the catalytic system developed in this study may be useful for the preparation of other beneficial heterocyclic compounds under environmentally benign conditions.

Two-Stage Sequential Adsorption System for Denitrogenation and Desulfurization of Model Diesel Oil over Ion-Exchanged Y Zeolites

This work focused on adsorptive desulfurization (ADS) and denitrogenation (ADN) in single- and two-stage systems. Na-Y zeolite possessed the best 4,6-DMDBT and DBT adsorption performances. La-Y zeolite exhibited the best ADN efficiency. The impact of nitrogen compounds and aromatics on ADS capacity and the impact of sulfur compounds and aromatics on ADN ability over ion-exchanged Y zeolite were evaluated in the single-stage system. The nitrogen compounds not only have the ability to compete, but they also have a higher absorbability than sulfur compounds. The sequential adsorption in a two-stage system was proposed to enhance ADS ability. The first stage contained La-Y for ADN, and the second stage contained Na-Y for ADS. It was found to successfully increase the breakthrough capacity of all compounds by 40% compared with the single-stage system. This two-stage sequential system is a suitable route for the elimination of sulfur and nitrogen content in diesel oil.

Evaluation of alkaline and acidic modification of NaY zeolite for enhancing adsorptive removal of diclofenac sodium from aqueous solution

In the present study, facile and cost-effective modification methods, including alkali treatment and acid treatment, were evaluated to improve the adsorption capacity of NaY zeolite for diclofenac sodium (DFS) elimination from the aqueous solution. The performance of unmodified NaY, sodium hydroxide treated NaY (SHT-Y), hydrochloric acid treated NaY (HAT-Y), and sulfuric acid treated NaY (SAT-Y) was compared, followed by optimization of sulfuric acid concentration since sulfuric acid was recognized as the preferred modifying agent. The modified NaY with a concentration of 0.1 M of sulfuric acid exhibited the highest adsorption capacity. Analyses of FE-SEM, EDS, FTIR, BET, and XRD were used to determine the characteristics of NaY and treated zeolites. All treatments changed the Si/Al ratio of NaY. Silanol and bridging hydroxyl functional groups were detected in all zeolitic samples. Acidic modifications enhanced the specific surface area of NaY more than the alkali treatment. The influence of the major factors on the DFS adsorption, including adsorbent dosage, contact time, pH of the solution, and initial concentration of DFS was investigated and optimized in detail. The maximum adsorption capacity was obtained at pH 11 at the equilibrium time of 180 min. The hydrogen bond formation and cation-π interaction were recognized as DFS adsorption mechanisms. From the results of kinetic studies, the Elovich model has better agreement with experimental data, suggesting that the adsorbent surface is energetically heterogeneous and chemisorption leads the DFS uptake onto the adsorbent, indicative of hydrogen bond formation. In accordance with the equilibrium modeling results, the Sips isotherm better expresses the DFS adsorption mechanism onto the surface of the modified zeolite. According to the Langmuir isotherm model, the maximum adsorption capacity obtained for 0.2 g/L of treated zeolite was 117.77 mg/g. Eventually, sulfuric acid treatment was found as an effective and facile method for enhancing zeolite performance in DFS removal from aqueous solution.

Synthesis of NaY zeolites with exceptionally high SiO2/Al2O3 ratios via a dissolution-recrystallization process

Commercial HY zeolites with SiO2/Al2O3 ratios between 5.1 and 80 have been treated in alkaline solutions in the presence of crown ether molecules. If the zeolites are almost totally amorphized at room temperature due to severe desilication of the framework, they completely recrystallize within less than one day when temperature is increased to 110 °C. Extra-framework species likely present in HY precursors are reincorporated in the framework, providing NaY zeolites with exclusively 4-coordinated Al species. The composition of the newly formed NaY zeolite depends not only on the SiO2/Al2O3 ratio of the HY precursor but also on the alkalinity of the desilicating solution. For each HY, the SiO2/Al2O3 ratio of the recrystallized NaY increases linearly when the Na2O/SiO2 ratio of the alkaline solution decreases, down to a limit beneath which complete recrystallization is no longer possible. FAU zeolites with framework SiO2/Al2O3 ratios up to 25 have been obtained from CBV 760, which constitutes the highest value ever reported in the literature for as-made NaY zeolites. A mechanism involving adsorption of crown ether molecules in the supercages of the partially desilicated framework followed by recrystallization has been proposed. This mechanism supports all experimental results, in particular the presence of more than 1 crown ether molecule per supercage in recrystallized solids.

New developments in supercritical fluids as a green technology: Processing of β-glucanase with sub- and supercritical carbon dioxide

There is evidence that high pressure can lead to changes in the stability and activation of enzymes, even though the majority of cases reported the inactivation of harmful enzymes. Supercritical fluids have attracted attention recently in green biotechnological processes. In this study, the aim is to investigate the capability of supercritical carbon dioxide to positively alter the stability and activity of hydrolytic enzymes as an alternative technique. The effects of operational parameters such as pressure (60–300 bar), temperature (28–80 °C), CO2 flow rate (2–10 g/min) and time (60–180 min) were evaluated with respect to the activity and stability of β-glucanase. The highest activity of β-glucanase was measured as 18.39 μmol/mL/min at 240 bar, 90 min, 67 °C and 8 g/min flow rate with SC-CO2 applications. In addition, the most active processed enzyme was employed in 11 subsequent enzymatic reactions after being immobilized in NaY zeolite with 71 % efficiency. The increased activity of the enzyme and prolonged useful life by ensuring its reuse can create a competitive advantage in the industry.