Zarrouk Medium Research Articles

Low operating cost approach in Spirulina platensis bio-photovoltaic cell

Bio-photovoltaic (BPV) cells are receiving more attention due to their clean electricity generation, utilisation of renewable energy, self-sustainability, and their ability to reduce carbon footprint by imitating the microorganism metabolism in the natural environment. This paper studied the performance of BPV using Spirulina platensis (S. platensis) as a biocatalyst in the anode compartment, under intermittent and continuous illumination with a light supply of 1500 lux. Zarrouk medium (ZM), and fertiliser medium (FM) became the anolyte, with graphite brush as electrodes. S.platensis cultured in FM showed a growth rate of 0.02285 ± 0.004 Abs/day, which was 15% less than growth in the ZM (0.02713 ± 0.006 Abs/day). The average current density normalised by the projected surface area of electrode produced by BPV with ZM under intermittent illumination at the end of the test period (≈1.654 A/m2) was higher than that of continuous illumination (≈0.391 A/m2). Meanwhile, the average current density by BPV with FM under intermittent illumination at the end of the test period (≈2.027 A/m2) was higher than that of the continuous illumination (≈0.839 A/m2). By comparison of the polarisation curve and the current density of ZM sets and FM sets, it can be deduced that the FM can be used to replace ZM in BPV operations.

A novel two-phase bioprocess for the production of Arthrospira (Spirulina) maxima LJGR1 at pilot plant scale during different seasons and for phycocyanin induction under controlled conditions

A two-phase outdoor cultivation bioprocess for Arthrospira maxima LJGR1 combined with phycocyanin induction in concentrated cultures under controlled conditions was evaluated using a modified low-cost Zarrouk medium. Growth was monitored during 4 cycles in 2018 and 4 cycles in 2019. Biomass was harvested and concentrated using membrane technology at the end of each cycle for further phycocyanin induction using blue LED light (controlled conditions, 24 h). The highest biomass productivity was observed during spring and summer cycles (13.63–18.97 gDWm−2 d−1); during mid-fall and mid-end fall, a decrease was observed (9.93–7.76 gDWm−2 d−1). Under favorable growth conditions, phycocyanin induction was successful. However, during cycles with unfavorable growth condition, phycocyanin induction was not observed. Reactive-grade phycocyanin (3.72 ± 0.14) was recovered and purified using microfiltration and ultrafiltration technologies.

Biomass and nutritive value of Spirulina (Arthrospira fusiformis) cultivated in a cost-effective medium

IntroductionCultivation of spirulina at commercial-scales relies on analytical grade–based media, which are expensive and so are the product.PurposeThis study assessed the biomass, proximate composition, and other useful compounds in Spirulina (Arthrospira fusiformis) produced with a cost-effective culture medium (LCMA), and the results were compared with those from a standard Zarrouk medium–grown spirulina.MethodsThe LCMA medium was formulated by using a commercial NPK10-20-20 fertilizer as a source of the three major nutrients for spirulina growth, and other three ingredients from Zarrouk medium. The experiment was conducted for 28 days in the glass aquaria under indoor conditions. Standard analytical methods were applied for the determination of proximate composition, chlorophyll, minerals, and vitamins in the spirulina biomass.ResultThe LCMA medium showed the best growth conditions by accumulating higher chlorophyll content (0.99 ± 0.02%) and dry weight (0.75 ± 0.01 g/100 ml) as well as attaining higher optical density (2.06 at day 15) earlier than the Zarrouk medium. The results of the proximate analysis for spirulina cultured in the LCMA medium were of good quality, with the protein contributing more than 50% of its dry matter. It was further noticed that the LCMA was an ideal medium for optimization of vitamins and some minerals since it recorded a significant amount of most of the analyzed vitamins together with the minerals sodium and potassium compared with the Zarrouk medium.ConclusionIt is suggested that LCMA medium could be used as the alternative and cheap medium for maximization of biomass and production of useful biochemical compounds in spirulina species.

Biomass production and growth performance of Momela Lake’s spirulina (<i>Arthrospira fusiformis</i>) cultured under urea and N: P: K fertilizers as cheaper nitrogen sources

Spirulina species are world widely known for their ability to produce high quantities of useful metabolites such as protein, fatty acids and vitamins. One of the major challenges in production of this microalgae is the cost and composition of culture medium. In the present study, effect of two culture media with agricultural fertilizers as nitrogen sources viz. urea containing medium (UM) and N: P: K containing medium (NPKM) on production of a locally found spirulina (Arthrospira fusiformis) was investigated. Zarrouk medium (ZM) was used as a standard, other culture conditions included 67.5 μmol m-2 s-1 radiation, 10 pH and 30 ºC temperature. A. fusiformis was isolated from Momela Lake in Arusha National Park Tanzania. There was no difference (p > 0.05) in mean biomass production (BMmean) and doubling time (DT) between ZM (1.205 g/L, 1.437 days respectively) and UM (1.168 g/L, 1.63 days respectively). Significantly higher (p < 0.05) maximum biomass BMmax production and exponential growth rate (μ) in a sequential order was from ZM (1.844 g/L, 0.483/day respectively), UM (1.6 g/L, 0.425/day respectively) and NPKM (0.57 g/L, 0.273/day). It appeared that the wild-isolated A. fusiformis was able to grow well in less costly UM showing high potential for mass production.© 2019 International Formulae Group. All rights reserved.Keywords: Momela Lake’s spirulina, biomass production, agricultural fertilizers, nitrogen sources

Chemical Constituents of Zarrouk Medium Affect Growth, Pigments and Metabolites Productions of Spirulina platensis

GROWTH (OD, Dw), pigments (Chl. a, carotenoids) and metabolites (proteins, carbohydrates, phenolic, flavonoids) have been evaluated under different component concentrations of Zarrouk media (NaCl, NaNO3, K2HPO4 and K2SO4) as well as some heavy metals (Zn, Cu, Se). The normal concentration of the tested components in Zarrouk medium (control) was found to induce the maximum growth parameters, pigments and metabolites contents. Acetone extracts of the normal concentrations (1.0g/L NaCl, 2.5g/L NaNO3, 0.5g/L K2HPO4 and 1g/L K2SO4) revealed the highest phenolic contents. While the ethanolic extracts of starved media (0.0g/L) of N or S, P (0.2g/L) as well as high salinity (4g/L) showed the greatest flavonoid production. Concerning heavy metals, Zn enhanced all the investigated parameters more than those performed by Cu, Se and control sample. In absence of all heavy metals, both acetone and ethanolic extracts revealed the greatest phenolic and flavonoids contents.

Enhancement of C-phycocyanin productivity by Arthrospira platensis when growing on palm oil mill effluent in a two-stage semi-continuous cultivation mode

Palm oil mill effluent (POME) is well known as agricultural wastewater that has a high potential as a medium for microalgal growth due to its high macro- and micronutrient content. The cyanobacterium Arthrospira platensis is considered as a species with a high C-phycocyanin (C-PC) content which is important for fine chemical and pharmaceutical applications. However, cultivation of A. platensis on POME to produce economically feasible amounts of C-PC has not been well explored. For this, environmental, nutritional, and cultivation modes (batch, semi-continuous) were varied to optimize C-PC productivity when cultivated at various POME concentrations. Arthrospira platensis was found to grow well on POME. Highest biomass and C-PC concentrations were found on 30–100% POME. Central composite rotatable design (CCRD) response surface methodology demonstrated that C-PC productivity was influenced by urea addition at the optimum salinity. The highest C-PC productivity was found on 100% POME during semi-continuous cultivation, while the addition of phosphorus and urea did not significantly improve C-PC productivity. By applying semi-continuous cultivation with 50% POME at the first stage and 100% POME at the second stage, a similarly high C-PC productivity (4.08 ± 1.3 mg L−1 day−1) was achieved as compared with (artificial) Zarrouk medium during batch cultivation. We conclude that, when using a two-stage semi-continuous cultivation process, A. platensis can produce economically feasible amounts of C-PC when cultivated on 100% POME.

Growth medium and nitrogen stress sparked biochemical and carotenogenic alterations in Scenedesmus sp. CCNM 1028

Microalgae are a spring of valuable compounds finding its wide applicability as cosmo-nutraceuticals, a renewable substitute for bioenergy and bioremediation. The present study utilizes Scenedesmus sp. CCNM 1028, freshwater microalgae, to understand the influence of various microalgal growth media, like BG-11, Chu's 10, Bold's basal medium (BBM) and Zarrouk's media (ZM), on the growth and lutein production. Furthermore, two-stage nitrogen starvation studies were carried out using the selected BG-11 media and its effect on the lutein and biochemical parameters of Scenedesmus sp. CCNM 1028 after 0, 6, 9, 15 and 21 days (d) of nitrogen starvation were recorded. Lutein was accumulated till 6d of nitrogen starvation. Additionally, ~3-fold increase of carbohydrate content (%w/w) was observed after 21d of nitrogen starvation with respect to the control. Thus, this study proposes two-stage nitrogen starvation as a capable strategy to cultivate Scenedesmus sp. CCNM 1028 for lutein (6d) and carbohydrate production (21d).

Production and characterization of Spirulina sp. LEB 18 cultured in reused Zarrouk’s medium in a raceway-type bioreactor

The objective was to evaluate the effect of reusing Zarrouk's medium on a Spirulina sp. LEB 18 culture by determining kinetic parameters, chemical composition, biofuels, and thermal characterization. Cultivation was performed in a raceway bioreactor for 7 days, the supernatant was reused for four cycles. Culturing the microalga in the reused medium resulted in a cellular yield of 2.30 g L−1 (control) and 2.04, 1.89, 1.73, and 1.15 g L−1 for four cycles with no influence on cell growth and productivity. Biomass with high contents of carbohydrates (58.00%, 3rd cycle), phycocyanin (2.47 mg mL−1, 1st cycle), and saturated fatty acids (60.13%, 4th cycle) were obtained with an increase in the profiles of C16:0 (45.85%) and C18:2n6 (47.40%) in the 1st cycle. The reuse of Zarrouk's medium allowed obtaining biomass with reduced cost and differentiated characteristics, allowing the exploration of commercially important biomolecules by the completion of up to four cycles.

Nutritional optimization of Arthrospira platensis for starch and Total carbohydrates production.

Present study aims to optimize the production of starch and total carbohydrates from Arthrospira platensis. Growing concerns toward unprecedented environmental issues associated with plastic pollution has created a tremendous impetus to develop new biomaterials for the production of bioplastic. Starch-based biopolymers from algae serve as sustainable feedstock for thermoplastic starch production due to their abundant availability and low cost. A. platensis was cultivated in Zarrouk's medium at 32 ± 1°C and exposed to red light with a photoperiod of 12:12 hr light/dark. Growth kinetics studies showed that the maximum specific growth rate (μmax ) obtained was 0.059 day-1 with the doubling time (td ) of 11.748 days. Subsequently, Zarrouk's medium with different concentrations of sulfur, phosphorus and nitrogen was prepared to establish the nutrient-limiting conditions to enhance the accumulation of starch and total carbohydrates. In this study, the highest starch accumulated was 6.406 ± 0.622 mg L-1 under optimized phosphorus limitation (0.025 g L-1 ) conditions. Nitrogen limitation (0.250 g L-1 ) results demonstrated significant influenced (p < 0.05) on total carbohydrates (67.573 ± 2.893 mg L-1 ) accumulation in A. platensis. The starch accumulation in A. platensis was significantly affected (p < 0.05) by phosphorus limitation (0.0025 g L-1 ). Subsequently, the optimized phosphorus concentration was coupled with mixotrophic cultivation to further enhance the starch accumulation. The results obtained indicated that, the starch (11.426 ± 0.314 mg L-1 ) and carbohydrates (43.053 ± 2.986 mg L-1 ) concentration obtained was significantly high (p < 0.05) under mixotrophic cultivation. Therefore, it shown that nutrient limitation and mixotrophic cultivation are viable strategies to enhance the accumulation of starch and total carbohydrates in A. platensis.

Comparative study on the growth performance of Spirulina platensis on modifying culture media

India faces a severe challenge to ensure adequate nutrition for children and women. Chronic child nutrition deficiency is more prevalent in Madhya Pradesh state of India. Spirulina, multicellular and filamentous cyanobacterium are considered an absolute food supplement to combat malnutrition in Asian and African countries. Spirulina cultivation requires sufficient aeration, agitation and proper light intensity for enhanced biomass yield, cell productivity, specific growth rate, and protein content. This paper presents a novel experimental approach to maximize biomass yield, minimize evaporation rate and respiration losses in a laboratory scale closed reactor and open pond system. Lab scale open pond and closed reactor system were designed for spirulina cultivation under dry climatic conditions at Bhopal, India. Zarrouk media was used as standard and modified organic media was prepared by changing the nitrogen source. Temperature and other input parameters were maintained. Aeration was done manually in an open pond, and the air pump was used in the case of a closed reactor system. Biomass yield obtained from an open pond system was 11.34 g/l, and 12.28 g/l in the closed reactor system. Doubling time was also less in the closed reactor in comparison with the open pond system. Urea seems to be a promising alternative source of low-cost nitrogen for Spirulina cultures. From the experimental results, it is concluded that modified organic media and closed reactor system could be used for better biomass yield.

FEATURES OF SPIRULINA PLATENSIS CULTIVATED UNDER AUTOTROPHIC AND MIXOTROPHIC CONDITIONS

Cultivation of Spirulina platensis in Zarrouk media containing 0–20 g l-1 glucose was studied in a photobioreactor for 30 days using a light intensity of 3 klux. Various parameters were measured to evaluate the enhancement of cell performance with glucose such as cell number, osmolarity, membrane stability, biomass productivity, doubling time, stress intensity, stress tolerance, chlorophyll, protein, carbohydrates, and lipid contents. Based on the results, we concluded that S. platensis is able to grow and produce some ingredients in Zarrouk media containing up to 20 g l-1 of glucose which is the first to be reported. The cell concentration of the mixotrophic cultures (80 cells per mm2) corresponded well to the sum of the autotrophic cell concentrations (50 cells per mm2), showing that the addition of carbohydrate positively effects on the microalgae growth. The continuous operation supplemented with 0.5 g l-1 of glucose (G0.5) led to the maximum cell concentration about 9.06 g l-1 wet and 1.32 g l-1 dry weights. The highest tolerance index, specific growth rate, biomass productivity, cell division, osmolarity and membrane stability index were respectively 102.5%, 0.15 d-1, 0.04 g l-1d-1, 0.26 div d-1, 0.87 osmol kg-1 and 93.8%, obtained in the same treatment. Chlorophyll (6.7 % in G0; 0.046 g l-1 in G0.5), protein (79.9 % and 0.884 g l-1 in G0.5), carbohydrates (55.5% in G20; 0.492 g l-1 in G6) and lipid (53.3% in G10; 0.636 g l-1 in G0) percentages and yields were mostly enhanced in the mixotrophic condition. This study indicated that mixotrophic growth of S. platensis is useful for commercial biomass production.

Optimization of Spirulina platensis Biomass and Evaluation of its Protective Effect Against Chromosomal Aberrations of Bone Marrow Cells

There are numerous studies investigating the effectiveness and potential clinical applications of Spirulina in treating several diseases. So, the present essay was designed to Cultivate Spirulina sp. in three different media; namely (modified commercial low cost Zarrouk medium (MS), Khul medium and Sea Water enriched medium), to compare Biomass concentrations (g/lDW) and optical density (OD) for 20 days, and phytochemical screening (Total phenolic, total flavonoids and Antioxidant activity) of the crude extract of all media with commercial Spirulina (dry product). In addition, examine possible potentials of Spirulina against hepatic intoxication induced by CCl4 in albino male mice. Results revealed that, methanolic extract recorded the highest values in modified Zarrouk’s medium (88.98 mg gallic acid equivalent/ml, 78.57 mg Rutin equivalent/ml and 82.04%) in Total phenolic, total flavonoids and Antioxidant activity respectively. Conclusively, Spirulina showed an ameliorating effect of CCl4 induced chromosomal aberrations of bone marrow cells; which proves the protective role of it against the chromosomal damage.

PERTUMBUHAN POPULASI Spirulina platensis DALAM MEDIA LIMBAH CAIR BAHAN OLAHAN KECAP DAN MEDIA ZARROUK

ABSTRACTThe aims of this research were to study the influence of mixed industrial soy sauce liquid waste and Zarrouk medium mixture on population density and specific growth rate of Spirulina platensis on a short periode. This study has been conducted from March to April 2017 in the Laboratory of Aquaculture, Aquaculture study Program, Faculty of Agriculture, University of Sriwijaya. This research was arranged according to completely randomized design (CRD) with 5 treatment levels and 3 replications. The treatment levels consisted of P1 (0 % industrial soy sauce liquid waste + 100 % Zarrouk medium), P2 (25 % industrial soy sauce liquid waste + 75 % Zarrouk medium), P3 (50 % industrial soy sauce liquid waste + 50 % Zarrouk medium), P4 (75 % industrial soy sauce liquid waste + 25 % Zarrouk medium), P5 (100 % industrial soy sauce liquid waste + 0 % Zarrouk medium). The parameters observed during the study were population density and the maximum specific growth rate. The result of this study showed that the treatment P2 gave the best in maximum density (60.6 g.L-1 ) and specific growth rate (14.66%. day-1). Keywords: Spirulina platensis, the liquid waste ketchup, Zarrouk media.

Effectiveness of repeated usage of the modified Zarrouk culture medium for cultivation of Spirulina platensis

Spirulina (Arthrospira) platensis biomass has a wide range of applications in several industries. One of the key issues for its production is the reduction of the culture medium cost. Here we have shown the possibility of a single recycling (repeated usage) of the modified Zarrouk nutrient medium (MZM; which contains 8.4 g/l of NaHCO3and 0.1 g/l of NaOH instead of 16.8 g/l of NaHCO3) for spirulina cultivation without altering culture productivity, photosynthetic pigments and protein contents in its biomass given that technological approach consisting in application of a mixture of a freshly prepared and once recycled modified Zarrouk medium in a ratio of 1:1 by volume is used. The obtained results can be applied for further reduction of costs of spirulina biomass production compared to methods described in previous studies.

Cyanobacterial Biomass by Reuse of Wastewater-Containing Hypochlorite

Spirulina (Arthrospira) are cyanobacteria with high potential to use waste as a substrate for conversion into products such as biofuels and biopolymers. This study aims to investigate the alternative application of polyhydroxybutyrate (PHB) extraction wastes from cyanobacterial biomass in the cultivation of Spirulina sp. LEB 18. Cultures were prepared with the addition of waste at concentrations of 2, 4, 6, 8, and 10% (v/v) in Zarrouk medium. The experimental cultures were compared to the control culture (without the addition of waste). The waste was exposed to three conditions: no treatment, thermal treatment at 121°C for 15 min, and chemical neutralization by sodium thiosulfate. The maximum biomass concentrations achieved by the cultures on the control, with no treatment, with thermal treatment and with neutralized waste, were 1.15 g/L (0% waste), 0.96 g/L (8% waste), 1.2 g/L (6% waste), and 1.12 g/L (10% waste), respectively. The yield values obtained on the control, with no treatment, with thermal treatment, and with chemical neutralization were 0.07, 0.04, 0.08, and 0.08 g/L/d, respectively. These results show the usability of this waste in Spirulina sp. LEB 18 cultivation. The application of this waste reduces the disposal of waste in the environment.

English

Spirulina species are known to have a good nutritional profile and antioxidant properties against reactive oxygen species. However, little is known about the antioxidant contents and the scavenging ability of Arthrospira fusiformis, cultivated under various conditions. This study aimed at evaluating the content of antioxidants (total phenols, total flavonoids, β-carotene, and lycopene) and the activity of A. fusiformis produced using low-cost culture (LCMA) and standard culture (Zarrouk) media. The results revealed that A. fusiformis is rich in antioxidants and it possesses high scavenging and chelating activities. Interestingly, the LCMA was superior over the Zarrouk medium as it resulted in spirulina with a higher amount of antioxidants and lower EC50 values. In this context, production of natural antioxidants can be maximized through the use of cost-saving, inorganic culture medium. Key words: Arthrospira fusiformis, spirulina, total phenols, total flavonoids, carotenoids, scavenging activity, low-cost culture (LCMA) medium, Zarrouk medium.

Outdoor Cultivation of Spirulina platensis for Mass Production

In the present study, the blue-green alga Spirulina platensis (NRC) was used for mass production under outdoor cultivation in three open ponds with a final capacity of 75 m3 net cultivation volume. Subculturing was performed within sequences and gradual volumes till 1,200 L open plate photobioreactor. The first and second ponds (30 cm depth) were used for the actual continuous production, while the third pond (80 cm depth) was used as a continuous inoculum supplier. In spite of low turbulence of the third pond due to high depth, all ponds had the same mechanical specification concerning paddle wheel structure and turbulence rate (16 rpm). A final nutrient concentration was employed based on Zarrouk medium by commercial grade compounds with some modifications. The nutrition was performed for the third pond by extra supplementation of extra doses of macro and micro-nutrients during the production period and dilution took place when culture was transferred to production ponds (first and second). Each production pond was harvested every 48 hours and the remainder water was return again into the third pond. The harvested pond yielded about 40 kg per day of fresh algal weight containing about 85% moisture on a dry weight basis. The results proved that using urea as nitrogen and carbon source with corn steam liquor instead of sodium nitrate and low bicarbonate, reduces production cost and supports growth medium by an adequate amount of carbon dioxide on the expense of the luxury use of sodium bicarbonate (16.8 g.l-1). Chemical analysis of the produced biomass showed 58-62% crude protein, 6-8% of ether extract and 8-11% of total carbohydrates. S. platensis contained total essential amino acids (131.3 mg/g), with a predominance of arginine followed by glutamic acid, leucine and phenylalanine.

Influence of spectral light quality on the pigment concentrations and biomass productivity of Arthrospira platensis

The species in the Arthrospira genus are cultured at a large scale throughout the world primarily for food supplements for human and animal diets. These species are valued for the rich composition of their biomass, which contains minerals, antioxidants, proteins and essential amino acids. This biomass can also be used for high-value product extraction, for example, the pigments chlorophyll a, β-carotene and phycocyanin as well as essential fatty acids. The use of LEDs is a solution for optimizing the productivity and biochemical composition of biomass produced by these microalgae. In this study, an innovative strategy for using LEDs was evaluated for Arthrospira platensis cultivation to increase its biomass productivity and high-value pigments (chlorophyll a, total carotenoids and phycocyanin). Microalgae suspensions were cultured in 250 mL Erlenmeyer flasks containing 120 mL of culture (George-modified Zarrouk's medium, pH 8.90) at 32 ± 1 °C with constant stirring and an initial biomass concentration of 0.03 g L−1. The biomass weights from ten lighting conditions consisting of blue and red LEDs of different compositions were evaluated in relation to the algal productivity and the chlorophyll a, total carotenoid and phycocyanin contents. The best results were obtained using LEDs that had a 70% red and 30% blue composition and a light intensity of 100 μE m−2 s−1, leading to an average biomass productivity of 0.148 g L−1d−1 and average concentrations of 21.35 μg mL−1, 5.45 μg mL−1 and 167.98 μg mL−1 of chlorophyll a, carotenoids and phycocyanin, respectively, in the given culture volume.

Simultaneous production of phycobiliproteins and carbonic anhydrase by Spirulina platensis LEB‐52

AbstractThe simultaneous production of carbonic anhydrase and the phycobiliproteins, C‐phycocyanin and allophycocyanin, was evaluated during the cultivation of Spirulina platensis using Zarrouk's medium in its non‐diluted (100 % ZM) and diluted to 20 % (v/v) (20 % ZM) forms. In both media, Spirulina simultaneously produced carbonic anhydrase and phycobiliproteins. For the phycobiliproteins’ production, higher concentrations (mg/g) of C‐phycocyanin and allophycocyanin were obtained when 100 % ZM was used. For carbonic anhydrase production, higher concentrations (U/g) were obtained on the 6th day of cultivation in both culture media. 100 % ZM promoted greater productions of these bioproducts, and, for simultaneous obtainment, the cells should be harvested after 21 days, obtaining concentrations of 137.4 mg/g C‐phycocyanin, 104.4 mg/g allophycocyanin, and 26.6 U/g carbonic anhydrase.

Biomass composition of Arthrospira platensis during cultivation on industrial process water and harvesting

Microalgae have the ability to utilize nutrients from wastewater and use it for biomass production. The effluent from a biogas process was tested as a nutrient source for blue-green microalga Arthrospira platensis cultivation and compared with conventional synthetic medium. Cultivation was carried out in four different concentrations of industrial process water (25, 50, 75, and 100%). The biomass was then harvested by microfiltration, and centrifugation followed by freeze drying. Variations in biomass composition were studied, in order to investigate effects of industrial process water on A. platensis over 30 days of cultivation. Applied harvesting techniques were evaluated for their effect on physiochemical properties of the biomass. Arthrospira platensis was able to grow in all tested wastewater concentrations except 100%, however, increase of wastewater concentration in medium resulted in a decreased growth rate. Partial substitution of synthetic Zarrouk medium with 25% of wastewater showed no adverse effect on chemical composition of the biomass including high protein content (45–58% dry weight) and favorable fatty acid composition (42–45% PUFAs of total fatty acids). Evaluation by optical microscopy showed that microfiltration caused cell rupture at the moderate level while centrifugation had more severe effect on A. platensis. Effect of centrifugal forces and shear stress on A. platensis cells was confirmed by detecting lower lipid content in samples after applying both microfiltration and centrifugation due to cell content leakage.