Background: Uses and traditional knowledge (TK) are essential for the protection and management of natural resources. There are extensive records of traditional uses involving mainly vascular plants, while for mosses are scarce. This study documents the TK and uses of Dendropogonella rufescens in San Juan Luvina, Oaxaca, Mexico. Questions: For what purpose and how D. rufescens is used in San Juan Luvina? Can TK provide ecological information for this species? How is TK maintained among the members of the community? Studied species: Dendropogonella rufescens (Schimp.) Britt. (Bryophyta). Study site and dates: San Juan Luvina, Oaxaca, Mexico. March 2020. Methods: We conducted interviews with inhabitants from three age categories (15-41, 42-68, and 69-95 years). Interviews included questions about the length of time the community has used the plant, harvesting practices, traditional use per se, and plant recycling after traditional use. Results: Results include historical information about the use of the plant and TK grouped in seven categories (ceremonial, construction, craft, environmental, fuel, medicinal, and ornamental). These results include new reports of TK of bryophytes in Mexico. Conclusions: For the uses and TK reported, D. rufescens is essential for the cultural identity of the community of San Juan Luvina. D. rufescens TK could use to develop conservation strategies for bryophytes. As our findings suggest, TK and uses of bryophytes should be recorded because they are likely to disappear.