Zambian Population Research Articles

Characterization of mammalian orthoreoviruses isolated from faeces of pigs in Zambia.

Mammalian orthoreovirus (MRV) has been identified in humans, livestock and wild animals; this wide host range allows individual MRV to transmit into multiple species. Although several interspecies transmission and genetic reassortment events of MRVs among humans, livestock and wildlife have been reported, the genetic diversity and geographic distribution of MRVs in Africa are poorly understood. In this study, we report the first isolation and characterization of MRVs circulating in a pig population in Zambia. In our screening, MRV genomes were detected in 19.7 % (29/147) of faecal samples collected from pigs by reverse transcription PCR. Three infectious MRV strains (MRV-85, MRV-96 and MRV-117) were successfully isolated, and their complete genomes were sequenced. Recombination analyses based on the complete genome sequences of the isolated MRVs demonstrated that MRV-96 shared the S3 segment with a different MRV isolated from bats, and that the L1 and M3 segments of MRV-117 originated from bat and human MRVs, respectively. Our results suggest that the isolated MRVs emerged through genetic reassortment events with interspecies transmission. Given the lack of information regarding MRVs in Africa, further surveillance of MRVs circulating among humans, domestic animals and wildlife is required to assess potential risk for humans and animals.

Toward an Evolutionarily Appropriate Null Model: Jointly Inferring Demography and Purifying Selection.

The question of the relative evolutionary roles of adaptive and nonadaptive processes has been a central debate in population genetics for nearly a century. While advances have been made in the theoretical development of the underlying models, and statistical methods for estimating their parameters from large-scale genomic data, a framework for an appropriate null model remains elusive. A model incorporating evolutionary processes known to be in constant operation, genetic drift (as modulated by the demographic history of the population) and purifying selection, is lacking. Without such a null model, the role of adaptive processes in shaping within- and between-population variation may not be accurately assessed. Here, we investigate how population size changes and the strength of purifying selection affect patterns of variation at "neutral" sites near functional genomic components. We propose a novel statistical framework for jointly inferring the contribution of the relevant selective and demographic parameters. By means of extensive performance analyses, we quantify the utility of the approach, identify the most important statistics for parameter estimation, and compare the results with existing methods. Finally, we reanalyze genome-wide population-level data from a Zambian population of Drosophila melanogaster, and find that it has experienced a much slower rate of population growth than was inferred when the effects of purifying selection were neglected. Our approach represents an appropriate null model, against which the effects of positive selection can be assessed.

Variations in sex ratios estimated from census and survey data in Zambia, 1969-2014.

The sex ratio question has been an area of growing interest in population dynamics, especially in developing countries with respect to the issue of missing women, but few studies have examined this in sub-Saharan Africa. Both at birth and in the general population, sex ratios follow an expected demographic pattern in the absence of the interference of historical events affecting either males or females in a population. In Zambia, an unexpected demographic pattern of sex ratios is exhibited in census and survey data. This study used data from censuses and surveys conducted from 1969 to 2014 to examine variations in sex ratios in the Zambian population. It was found that sex ratio imbalances were largely due to data deficiencies due to age misreporting and under-enumeration. A consistent under-enumeration of young adult males in the 20-34 years age group was found. A systematic pattern of high sex ratios, above 100, for ages 40+ was found, represented by synthetic cohorts traceable from the 1969 census, progressing to the 2000 census and phased out in the 2010 census. Extremely high adult male mortality was found in 2010 in the 35-59 years age group, primarily attributable to HIV/AIDS. Understanding the demographic pattern of sex ratios in a population is relevant for policies to improve the quality of data collection systems, and socioeconomic development planning, for the young age group population, which is prone under-enumeration.

Eligibility for hepatitis B antiviral therapy among adults in the general population in Zambia.

We evaluated antiviral therapy (AVT) eligibility in a population-based sample of adults with chronic hepatitis B virus (HBV) infection in Zambia. Using a household survey, adults (18+ years) were tested for hepatitis B surface antigen (HBsAg). Sociodemographic correlates of HBsAg-positivity were identified with multivariable regression. HBsAg-positive individuals were referred to a central hospital for physical examination, elastography, and phlebotomy for HBV DNA, hepatitis B e antigen, serum transaminases, platelet count, and HIV-1/2 antibody. We determined the proportion of HBV monoinfected adults eligible for antiviral therapy (AVT) based on European Association for the Study of the Liver (EASL) 2017 guidelines. We also evaluated the performance of two alternative criteria developed for use in sub-Saharan Africa, the World Health Organization (WHO) and Treat-B guidelines. Across 12 urban and 4 rural communities, 4,961 adults (62.9% female) were tested and 182 (3.7%) were HBsAg-positive, 80% of whom attended hospital follow-up. HBsAg-positivity was higher among men (adjusted odds ratio [AOR], 1.37; 95% confidence interval [CI], 0.99-1.87) and with decreasing income (AOR, 0.89 per household asset; 95% CI, 0.81-0.98). Trends toward higher HBsAg-positivity were also seen at ages 30-39 years (AOR, 2.11; 95% CI, 0.96-4.63) and among pregnant women (AOR, 1.74; 95% CI, 0.93-3.25). Among HBV monoinfected individuals (i.e., HIV-negative) evaluated for AVT, median age was 31 years, 24.6% were HBeAg-positive, and 27.9% had HBV DNA >2,000 IU/ml. AVT-eligibility was 17.0% by EASL, 10.2% by WHO, and 31.1% by Treat-B. Men had increased odds of eligibility. WHO (area under the receiver operating curve [AUROC], 0.68) and Treat-B criteria (AUROC, 0.76) had modest accuracy. Fourteen percent of HBsAg-positive individuals were HIV coinfection, and most coinfected individuals were taking tenofovir-containing antiretroviral therapy (ART). Approximately 1 in 6 HBV monoinfected adults in the general population in Zambia may be AVT-eligible. Men should be a major focus of hepatitis B diagnosis and treatment. Further development and evaluation of HBV treatment criteria for resource-limited settings is needed. In settings with overlapping HIV and HBV epidemics, scale-up of ART has contributed towards hepatitis B elimination.

A Cross - Section Study to Determine Human Height Using Femur Length in Zambian Population

A Cross - Section Study to Determine Human Height Using Femur Length in Zambian Population

Voluntary medical male circumcision and HIV in Zambia: expectations and observations.

The study analysed the HIV/AIDS situation in Zambia six years after the onset of mass campaigns of Voluntary Medical Male Circumcision (VMMC). The analysis was based on data from Demographic and Health Surveys (DHS) conducted in 2001, 2007 and 2013. Results show that HIV prevalence among men aged 15-29 (the target group for VMMC) did not decrease over the period, despite a decline in HIV prevalence among women of the same age group (most of their partners). Correlations between male circumcision and HIV prevalence were positive for a variety of socioeconomic groups (urban residence, province of residence, level of education, ethnicity). In a multivariate analysis, based on the 2013 DHS survey, circumcised men were found to have the same level of infection as uncircumcised men, after controlling for age, sexual behaviour and socioeconomic status. Lastly, circumcised men tended to have somewhat riskier sexual behaviour than uncircumcised men. This study, based on large representative samples of the Zambian population, questions the current strategy of mass circumcision campaigns in southern and eastern Africa.

Anthropometrics Analysis of Mental Foramen and Accessory Mental Foramen in Zambian Adult Human Mandibles.

The mental foramen (MF) and accessory mental foramen (AMF) are the strategically important landmarks during surgical interventions and anaesthetic nerve blocks procedures involving the mental nerve. The study aimed at evaluating anthropometrics of MF and AMF in Zambian adult human mandibles and it was cleared for ethics from TDRC Ethics Review Committee (Reg. No.: 00002911; FWA: 00003729). A total of 33 Zambian adult human mandibles were evaluated for shape, position, and direction of opening of foramen. All measurements were performed using a Digital Vernier Calliper and statistically analysed for per cent frequency and mean and standard deviations, and we performed the one sample t-test for comparative analysis. Data were considered significant at p<0.05. All mandibles that were examined had bilateral MF while unilateral AMF was found in two mandibles (6%). The foramens were mostly oval in shape and their most common position was between the second premolar and first molar and the most common orientation was posterior-superior. The comparative analysis of mandibular anthropometrics showed significant variations (p<0.05) with different ethnic groups. The findings emphasize the ethnic variations and edify that the foramen position is not always as stated in reference textbooks. The clinical creditability of the study is cautioning the surgeons on possible variations of the MF and AMF anthropometrics compared to existing literature in order to avoid any unforeseen injury related to anaesthesia or dental surgeries. Further studies with large sample sizes representing whole country are recommended to establish the standard MF and AMF anthropometrics of Zambian population.

An Assessment of the Challenges affecting Smallholder Farmers in Adopting Biogas Technology in Zambia

The objective of this study was to assess the challenges of adopting biogas technology among rural households in Zambia. Three hundred and twenty households with and without biodigesters were selected randomly from five provinces in the country for the study. A household survey and qualitative methods such as focus group discussions and key interviews were used to collect information. The results show that firewood and charcoal are the main sources of cooking energy in the study areas despite the enormous potential for the utilization of biogas. The use of biogas technology is in its infancy and few households have adopted the technology. The study found that several challenges or factors were responsible for the low adoption status of biogas technology in the study areas. The main challenges were the high cost of installation of biodigesters; lack or limited access to credit to help meet the costs of construction of biodigesters; inadequate numbers of skilled biodigester technicians; and lack of awareness or limited information on biogas technology. Increasing access to affordable credit, as well as awareness raising on biogas technology among rural households are suggested as some of the ways that will assist to promote the adoption of biogas technology as a sustainable renewable energy source for rural populations in Zambia.

The anatomy of the Lamina terminalis and Cisterna Chiasmatica: A cadaveric study at the University Teaching Hospital, Lusaka, Zambia

The Lamina Terminalis represents an important neuroanatomical structure by which third Ventriculostomy could be performed into the subarachnoid space through the Cisterna Chiasmatica. Recent studies have indicated a role for Lamina Terminalis fenestration in approaching pathologies of the third ventricle. However, there is limited knowledge on the anatomical features, variations and the vasculature of the Lamina Terminalis in a Zambian population. This study was aimed to explore the anatomy of the Lamina Terminalis, the Cisterna Chiasmatica and its neurovascular relationships as seen in a Zambian population and compare with the findings in the literature. The study was a descriptive cross-sectional design in which 32 post-mortem human cadaveric brains were systematically sampled. The Lamina Terminalis region was examined in 27 male cadavers and five female cadavers of age range between 25 and 66 years (mean 34.1 ± 9.2years). Data was collected using a data collection form, entered and analyzed by descriptive statistics using Statistical Package for Social Sciences (SPSS) software version 22. Mean and standard deviations were used to describe variables. The base of the brain was detached from the floor of the cranial cavity and the Lamina Terminalis exposed fully by retracting both optic nerves and the optic Chiasma posteroinferiorly. Thetriangular Lamina Terminalis measurements were performed as the distance between the midportion of the upper aspect of the chiasma and the lower aspect of the anterior commissure (height), which averaged 8.6 ±1.0mm. The distance between the medial edges of the optic tracts (base) averaged 13.1 ±1.1mm and the area averaged 56.9 ± 11.6mm2. The Lamina Terminalis membrane showed variations in appearance; 20 were transparent with a large dark midline gap and 10 were less translucent with a slender midline gap and two were indistinct. The Cisterna Chiasmatica was observed as a dilated subarachnoid space adjacent to the Optic Chiasma. For neurovascular relationships, 24 cadavers showed arterioles arising from the posterosuperior aspect of the anterior cerebral artery to perforate the anterior perforating substance(s), supplying the chiasma, and optic tracts; whereas, eight cadavers showed arterioles arising posteroinferiorly and ramifying on the Lamina Terminalis. The Lamina Terminalis membrane is variably developed. There are variations in the membrane appearance, measurements and neurovascular relationships. These findings will supplement a knowledge gap in neuroanatomy and help prevent complications during Lamina Terminalis fenestration in approaching pathologies of the third ventricle.Key words: Lamina Terminalis, Cisterna Chiasmatica, Third Ventriculostomy

Applying the social determinants of health lens to the situation of young key populations in Zambia: what can it tell us about what we could do?

# Background According to census data, Zambia has a predominantly young population with 65% under the age of 25 years. Improving the sexual and reproductive health of young people aged 15-24 years has become a crossing-cutting priority for sustainable development for the country. Certain sub-groups within this population, namely young gay men and other men-having-sex-with-men; young sex workers; young transgender people; and young prisoners -- collectively termed key populations -- may have poorer sexual and reproductive health than their general population peers. The application of the social determinants of health framework offers a way to understand these inequities in order to more effectively address them. # Methods Data and other information to support this analysis were collected using key informant interviews and desk review. Interviews were conducted with 30 individuals deemed to be knowledgeable about the situation of young key populations in Zambia. Peer-reviewed literature on these groups is very limited. As a result, non-peer-reviewed sources were also used. # Results According to the limited information that is available, young key populations do indeed have significantly poorer sexual reproductive health, particularly elevated rates of HIV and other sexually transmitted infections. This may arise due to a range of determinants linked to a general context of marginalisation and disadvantage, as well as the more specific pathways related to the effects of punitive laws and policies; negative socio-cultural beliefs and practices; a high degree of social exclusion; and limited or no access to relevant and acceptable health services. While, in this last regard, tailored HIV-focussed programming is beginning to expand for some groups, it is not yet addressing these broader forces of risk and vulnerability. # Conclusion The growing attention to young key populations in Zambia is both appropriate and necessary. However, more research is urgently needed to guide this emphasis. The social determinants of health can provide a highly relevant organising framework for this work. Should further investigation confirm some of the conclusions of this analysis, more will be needed to achieve health equity for young key populations than tailored services alone. Enabling, inclusive, and protective legal, policy and programmatic approaches will be equally critical.

Applying the social determinants of health lens to the situation of young key populations in Zambia: what can it tell us about what we could do?

Applying the social determinants of health lens to the situation of young key populations in Zambia: what can it tell us about what we could do?

Trade in Zambian Edible Orchids-DNA Barcoding Reveals the Use of Unexpected Orchid Taxa for Chikanda.

In Zambia, wild edible terrestrial orchids are used to produce a local delicacy called chikanda, which has become increasingly popular throughout the country. Commercialization puts orchid populations in Zambia and neighbouring countries at risk of overharvesting. Hitherto, no study has documented which orchid species are traded on local markets, as orchid tubers are difficult to identify morphologically. In this study, the core land-plant DNA barcoding markers rbcL and matK were used in combination with nrITS to determine which species were sold in Zambian markets. Eighty-two interviews were conducted to determine harvesting areas, as well as possible sustainability concerns. By using nrITS DNA barcoding, a total of 16 orchid species in six different genera could be identified. Both rbcL and matK proved suitable to identify the tubers up to the genus or family level. Disa robusta, Platycoryne crocea and Satyrium buchananii were identified most frequently and three previously undocumented species were encountered on the market. Few orchid species are currently listed on the global International Union for the Conservation of Nature (IUCN) Red List. Local orchid populations and endemic species could be at risk of overharvesting due to the intensive and indiscriminate harvesting of chikanda orchids, and we therefore encourage increased conservation assessment of terrestrial African orchids.

A retrospective analysis of adverse obstetric and perinatal outcomes in adolescent pregnancy: the case of Luapula Province, Zambia

BackgroundAbout three in ten young women aged 15–19 have begun childbearing among the Zambian population, with adolescent pregnancy levels as high as 35% in rural areas. In 2009, Luapula reported 32.1% adolescent pregnancies. The study sought to investigate obstetric and perinatal outcomes among adolescents compared to mothers aged 20-24 years delivering at selected health facilities in Kawambwa and Mansa districts of Luapula.MethodsA retrospective analysis was carried out of all deliveries to mothers aged between 10 and 24 years for the period January 2012 to January 2013. A total of 2795 antenatal and delivery records were reviewed; 1291 adolescent mothers and 1504 mothers aged 20–24 years. Crude and adjusted odds ratios for the association between maternal age and adverse obstetric and perinatal outcomes were obtained using logistic regression models.ResultsThe mean age of the adolescent mothers was 17.5 years. Mothers younger than 20 years faced a higher risk for eclampsia, anaemia, haemorrhage, Cephalopelvic disproportion, prolonged labour and caesarean section. After adjustment for potential confounders, the association between maternal age and adverse obstetric and perinatal outcome diminished. Children born to mothers younger than 20 were at increased risk for low birth weight, pre-term delivery, low Apgar score and neonatal death; the risk for asphyxia, however, tended to increase with age.ConclusionThe findings demonstrate that adolescent pregnancy increases the risk of adverse obstetric and perinatal outcomes. High rates of adolescent pregnancies in Luapula province are likely as a result of the predominantly rural and poor population. Understanding the factors that contribute to the high levels of adolescent pregnancy in the region will be vital in addressing the situation and subsequently reducing the high obstetric and perinatal morbidity and mortality.

Barriers to safe-sex behavior change in Zambia: Perspectives from HIV/AIDS psychosocial counselors

Despite being one of the Sub-Saharan African countries most affected by HIV/AIDS, few studies address the mismatch between continued practices of high-risk sexual behaviors considering increased HIV/AIDS knowledge in Zambia. This article reports findings from an exploratory qualitative study that addressed the research question, “What are the attitudinal, cultural, interpersonal, and contextual barriers to safer sexual behavior change among the Zambian population?” The study involved semistructured interviews with eight psychosocial counselors from two Zambian cities who provide HIV testing and counseling services. Data were analyzed using the constant comparative method. Findings indicated individual level barriers including gendered differences, adolescent specific barriers, and issues related to illiteracy. Lack of communication and perceived meaning of condom use emerged as factors influencing unsafe sex at partner levels. Community level factors included high rates of poverty, substance abuse, availability of antiretroviral therapy, inconsistent condom supplies, cultural beliefs, unemployment, and limited recreational activities.

Quality of care and HIV service utilization among key populations in Zambia: a qualitative comparative analysis among female sex workers, men who have sex with men and people who use drugs

ABSTRACTHIV epidemic control requires improving access and uptake of HIV services by key populations (KPs). In Zambia, the behaviors of female sex workers (FSWs), men who have sex with men (MSM), and people of who use drugs (PWUD) are criminalized, and little information exists about their HIV/STI service use. Using a quality of care (QOC) framework, we compared barriers to and opportunities for HIV/STI service access and uptake among the three KPs. We conducted in-depth interviews and focus group discussions with 314 KP members between July 2013 and September 2015 in eight districts. Poorer QOC was received at public health facilities compared to private, NGOs and traditional healers. Stigma and discrimination, confidentiality, and legal prosecution were barriers to service use and more salient among MSM than FSWs and PWUD. Invasive facility policies were barriers and more prominent among FSWs than MSM and PWUD. Service unavailability was of equally high salience among MSM and PWUD than FSWs. Comfort in the clinic and perceived treatment effectiveness were facilitators for all three KPs. The health care experiences of KPs are not monolithic; HIV/STI service improvement strategies should address the concerns and be tailored to the needs of each key population.

Retracted: Cadaveric study on variations of the cystic artery in a Zambian population

This article has been retracted by the authors. An incorrect dataset was used, therefore the resullts are misleading.The main source of blood supply to the gall bladder is the cystic artery which is a branch of the right hepatic artery. Anatomical variations of the cystic artery are frequent. Thus, careful dissection of the Calot`s triangle is necessary for conventional and laparoscopic cholecystectomy. The knowledge of variations of the origin, course, and length of the cystic artery is important for the surgeon as bleeding from the cystic artery during cholecystectomy can lead to death. Forty-three post-mortem human cadavers at the University Teaching Hospitals, Pathology Department, Lusaka were dissected and examined over a period of five weeks, to establish the origin, length and course of the cystic artery. And to establish the relationship of the cystic artery to the cystic duct. Out of the 43 human cadavers, the cystic artery was found to be originating from the right hepatic artery in 37 cases (86%), from hepatic artery proper in four cases (9.3%), from the left hepatic artery in one case (2.3%) and liver parenchyma in one case (2.3%). In the forty (93%) cadavers dissected, only one cystic artery was identified and in three (7%) others there were two arteries detected. The cystic artery length ranged from 2 – 6 cm (mean 3.53± 0.962 cm). The cystic artery was found to be inside Calot`s triangle in 37 cases (86%) while in four cases (9.3%) it was outside the Calot`s triangle. The cystic artery was found to be passing supero-medial to the cystic duct in 40 cases (93%) while in two cases (4.7%) the cystic artery was passing anterior to the cystic duct and one (2.3%) was unrelated (from the gall bladder bed) to the cystic duct. Demographic characteristic (gender and age) had no statistically significant association to variations of cystic artery (p&gt;0.005). Six (16.2%) of males had variants of cystic artery and no females had variants of the cystic artery. Variations of the cystic artery origin, length, its course through the Calot`s triangle and its relation to the cystic duct are common. Knowledge of these variations is important and helpful in preventing and controlling haemorrhage or avoiding other complications during conventional and laparoscopic cholecystectomy.Keywords: Cystic artery, Cholecystectomy, Calot`s triangle

Implementation of a community-based intervention in the most rural and remote districts of Zambia: a process evaluation of safe motherhood action groups

BackgroundA community-based intervention known as Safe Motherhood Action Groups (SMAGs) was implemented to increase coverage of maternal and neonatal health (MNH) services among the poorest and most remote populations in Zambia. While the outcome evaluation demonstrated statistically significant improvement in the MNH indicators, targets for key indicators were not achieved, and reasons for this shortfall were not known. This study was aimed at understanding why the targeted key indicators for MNH services were not achieved.MethodsA process evaluation, in accordance with the Medical Research Council (MRC) framework, was conducted in two selected rural districts of Zambia using qualitative approaches. Focus group discussions were conducted with SMAGs, volunteer community health workers, and mothers and in-depth interviews with healthcare providers. Content analysis was done.ResultsWe found that SMAGs implemented much of the intervention as was intended, particularly in the area of women’s education and referral to health facilities for skilled MNH services. The SMAGs went beyond their prescribed roles to assist women with household chores and personal problems and used their own resources to enhance the success of the intervention. Deficiencies in the intervention were reported and included poor ongoing support, inadequate supplies and lack of effective transportation such as bicycles needed for the SMAGs to facilitate their work. Factors external to the intervention, such as inadequacy of health services and skilled healthcare providers in facilities where SMAGs referred mothers and poor geographical access, may have led SMAGs to engage in the unintended role of conducting deliveries, thus compromising the outcome of the intervention.ConclusionWe found evidence suggesting that although SMAGs continue to play pivotal roles in contribution towards accelerated coverage of MNH services among hard-to-reach populations, they are unable to meet some of the critical sets of MNH service-targeted indicators. The complexities of the implementation mechanisms coupled with the presence of setting specific socio-cultural and geographical contextual factors could partially explain this failure. This suggests a need for innovating existing implementation strategies so as to help SMAGs and any other community health system champions to effectively respond to MNH needs of most-at-risk women and promote universal health coverage targeting hard-to-reach groups.

DEFB1 polymorphisms and HIV-1 mother-to-child transmission in Zambian population

Introduction: Human Beta Defensin-1 (hBD-1) is a component of the innate immune system, the first line of defence against pathogens, already reported as involved in the susceptibility to HIV-1 infection and HIV-1 mother-to-child transmission (MTCT) in different populations. We investigated the role of DEFB1 gene (encoding for hBD-1) functional polymorphisms in the susceptibility to HIV-1 MTCT in a population from Zambia.Methods: Four selected polymorphisms within DEFB1 gene, three at the 5′ untranslated region (UTR), namely -52G > A (rs1799946), -44C > G (rs1800972) and -20G > A (rs11362) and one in the 3′UTR, c.*87A > G (rs1800972), were genotyped in 101 HIV-1 positive mothers (26 transmitters -27% and 75 not transmitters -73%) and 331 infants born to HIV-1 infected mothers (85 HIV-1 positive -26% and 246 exposed but not infected -74%).Results: DEFB1 c.*87–A allele was more frequent among HIV− children with respect to HIV+ (with intrauterine MTCT). Concerning DEFB1 haplotypes, GCGA haplotype resulted more represented in HIV− than HIV+ infants and DEFB1 ACGG haplotype presented increased frequency in HIV− children respect to HIV+ (with intra-partum MTCT) (p = .02, p = .002 and p = .006, respectively).Conclusions: DEFB1 polymorphisms were significantly associated with decreased risk of HIV-1 infection acquisition in the studied Zambian population suggesting that they may play a role in HIV-1 MTCT.

Deprivation levels in Zambia, 2006 – 2015: an analytical profile

Zambia has registered impressive economic growth rates since the beginning of this millennium. The question is whether this has resulted in commensurate impacts on reduction in deprivation suffered by a significant percentage of the Zambian population. Has the growth been pro-poor and pro-vulnerable? This paper explored this question by examining the changes in the levels of deprivation during the period 2006-2015. The methodology involved the construction of indices of deprivation based on 15 selected variables and the construction of an aggregate measure of deprivation using these indices. The data used are obtained from the Zambia Living Conditions Monitoring Survey reports for 2006, 2010 and 2015. Based on the results obtained, some key conclusions and policy suggestions have been made. Keywords: Economic growth, poverty, vulnerability, deprivation index, inequality

Are Nonsense Alleles of Drosophila melanogaster Genes under Any Selection?

A gene which carries a bona fide loss-of-function mutation effectively becomes a functionless pseudogene, free from selective constraint. However, there is a number of molecular mechanisms that may lead to at least a partial preservation of the function of genes carrying even drastic alleles. We performed a direct measurement of the strength of negative selection acting on nonsense alleles of protein-coding genes in the Zambian population of Drosophila melanogaster. Within those exons that carry nonsense mutations, negative selection, assayed by the ratio of missense over synonymous nucleotide diversity levels, appears to be absent, consistent with total loss of function. In other exons of nonsense alleles, negative selection was deeply relaxed but likely not completely absent, and the per site number of missense alleles declined significantly with the distance from the premature stop codon. This pattern may be due to alternative splicing which preserves function of some isoforms of nonsense alleles of genes.