Three sesquiterpene glycosides were isolated from the MeOH (70%) extract of flowers of Yucca gloriosa L. Their chemical structures were established using spectral analyses, i.e., 1D and 2D NMR (1H, 13C, HSQC, HMBC, COSY) and mass spectroscopy (ESI-MS). They all were new glycoside derivatives of nerolidol and a new class for the genus Yucca. Glycoside 1 had the structure (1E,3S,5R,6E,9E)-5-O-β-D-glucopyranosyl-(1_6)-O-β-D-glucopyranosyl-3,5,11-trihydroxy-3,7,11-trimethyldodeca-1,6,9-triene; glycoside 2, (1E,3S,5R,6E,9E)-5-O-β-D-glucopyranosyl-(1→6)-O-β-D-glucopyranosyl-3,5,11,12-tetrahydroxy-3,7,11-trimethyldodeca-1,6,9-triene; glycoside 3, (1E,3S,5R,6E,10E)-(12-O-β-D-glucopyranosyl)-5-O-β-Dglucopyranosyl-(1→6)-O-β-D-glucopyranosyl-3,5,12-trihydroxy-3,7,11-trimethyldodeca-1,6,10-triene.