Padrón-Cabo, A, Lorenzo-Martínez, M, De Dios-Álvarez, V, Rey, E, and Solleiro-Durán, D. Effects of a short-term detraining period on the physical fitness in elite youth soccer players: a comparison between chronological age groups. J Strength Cond Res XX(X): 000-000, 2024-The aim of this study was to examine the effects of a 2-week in-season training cessation on neuromuscular and endurance performance in 2 different age groups of elite youth soccer players. A total of 30 players belonging to the under-15 (U15, n = 17) and under-17 (U17, n = 13) age groups of a Spanish professional soccer academy participated in the study. The following tests were assessed before and after the training cessation period: squat jump (SJ), countermovement jump (CMJ), 20-m sprint with 5-m and 10-m split times, modified 505 change of direction, and 30-15 Intermittent Fitness Test (30-15 IFT). The results showed significant and similar performance impairments between the 2 age groups in SJ (p = 0.020), CMJ (p = 0.044), 20-m sprint (p < 0.001), and 30-15 IFT (p < 0.001). The U17 players evidenced greater impairment than the U15 players in 5-m (p = 0.006) and 10-m (p = 0.002) sprint performance. However, there was no significant change (p = 0.070) in the players' change of direction performance. These findings provide valuable information for strength and conditioning coaches, suggesting the need for targeted interventions to mitigate the effects of detraining on neuromuscular and endurance performance during short-term in-season training cessation periods in elite youth soccer players. Special attention should be paid to maintaining sprint performance during short-term detraining periods in U17 players.