Young Permanent Teeth Research Articles

Management of Delayed Reimplanted Avulsed Young Permanent Tooth by Decoronation: A Case Report

Management of Delayed Reimplanted Avulsed Young Permanent Tooth by Decoronation: A Case Report

English

BACKGROUND The vitality of dental pulp is essential for long-term survival of the tooth. Vital pulp therapy (VPT) intends to maintain healthy pulp tissue by eliminating bacteria from the dentin-pulp complex. There are many treatment options for vital pulp therapy in extensively decayed teeth. Pulp capping or pulpotomy procedures rely upon an accurate assessment of the pulp status, and careful management of the remaining pulp tissue. We wanted to evaluate as to whether biodentine total pulpotomy is an effective alternative to conventional endodontic therapy for young permanent teeth. METHODS Full coronal pulpotomy was performed in young permanent molars diagnosed with acute irreversible pulpitis with Biodentine as the pulpotomy medicament. Follow up evaluation was done clinically and radiographically at 3, 6 and 12 months. RESULTS During follow-up periods clinical signs/symptoms were absent including pulpal pain, swelling or presence of sinus and percussion pain. Continuous root development and healing of periapical radiolucency were noticed in the study which were indicative of maintenance of vitality of the pulp. CONCLUSIONS Within the limitations of this clinical study, it is concluded that total pulpotomy with Biodentine has a promising scope in regenerative approaches in the treatment of carious young permanent teeth. KEY WORDS Biodentine, Coronal Pulpotomy, Permanent Teeth, Total Pulpotomy

Assessment the Efficacy of Resin Infiltration in Treatment of Young Permanent Teeth

Purpose: To evaluate the efficacy of resin infiltration in treatment of young permanent teeth and to evaluate dental anxiety related to resin infiltration procedure and the time required. Material and methods: 22 Egyptian children ranging from 6-14 years of age were selected with at least 2 smooth surface white lesions (WSL), one on each side using split mouth design. Lesions were assigned in a random way into 2 groups; Group I, Group II. Group I: resin infiltration, Group II: fluoride varnish. Radiographic and Photographic records were taken before treatment, immediately after treatment and 12 months plus assessing the marginal adaptation and discoloration. A facial image scale (FIS) was recorded. The outcome data were recorded, tabulated, coded to be statistically analyzed. Results: by radiographic evaluation immediately after treatment and through the study period the difference was statistically significant in the mean of DSR and RD between groups in favor of group I. By the descriptive analysis the difference of mean of change in both marginal adaptation and discoloration was not a statistically significant. While group I, show increase in mean of marginal adaptation but did not reach to significance level. But within group II, the mean of discoloration has shown statistically significantly difference. Dental anxiety was low in both groups with no statistically significantly difference. Conclusion: Infiltration concept was bridging the gap between “wait and see”; it is a promising micro-invasive approach.

Roles of Dental Mesenchymal Stem Cells in the Management of Immature Necrotic Permanent Teeth.

Dental caries and trauma always lead to pulp necrosis and subsequent root development arrest of young permanent teeth. The traditional treatment, apexification, with the absence of further root formation, results in abnormal root morphology and compromises long-term prognosis. Regeneration endodontics procedures (REPs) have been developed and considered as an alternative strategy for management of immature permanent teeth with pulpal necrosis, including cell-free and cell-based REPs. Cell-free REPs, including revascularization and cell homing with molecules recruiting endogenous mesenchymal stem cells (MSCs), have been widely applied in clinical treatment, showing optimistic periapical lesion healing and continued root development. However, the regenerated pulp–dentin complex is still absent in these cases. Dental MSCs, as one of the essentials of tissue engineering, are vital seed cells in regenerative medicine. Dental MSC–based REPs have presented promising potential with pulp–dentin regeneration in large animal studies and clinical trials via cell transplantation. In the present review, we summarize current understanding of the biological basis of clinical treatments for immature necrotic permanent teeth and the roles of dental MSCs during this process and update the progress of MSC-based REPs in the administration of immature necrotic permanent teeth.

Apexification with apical growth and closure using Metapex in a Necrotic Immature Permanent Tooth with Periapical abscess: A Case Report with 16 months follow-up.

The purpose of this report is to present the case of a patient wherein apexification of an immature permanent maxillary left central incisor tooth was induced by the Metapex paste (Calcium hydroxide and Iodoform). It has been reported in the literature of dentistry that apexification treatment in teeth with open apex is more than 40 years old. Apexification procedures had been carried out in different ways using various techniques and materials. Regardless of the type of materials used for apexification method by different authors, apexification procedures appear to be the most subtle and accepted clinical practice in non-vital young permanent teeth. In our case, radiographic examination showed the start of apical closure 6 months after the completion of the treatment protocol. The growth of root end and complete apical closure was confirmed 16 months after the treatment, indicating the implausible potential of Metapex to induce apical growth and closure in young permanent tooth with periapical lesion.

Adipose Tissue–derived Microvascular Fragments as Vascularization Units for Dental Pulp Regeneration

IntroductionThe transplantation of dental pulp stem cells (DPSCs) has emerged as a novel strategy for the regeneration of lost dental pulp after pulpitis and trauma. Dental pulp regeneration of the young permanent tooth with a wide tooth apical foramen has achieved significant progress in the clinical trials. However, because of the narrow apical foramen, dental pulp regeneration in adult teeth using stem cells remains difficult in the clinic. Finding out how to promote vascular reconstitution is essential for the survival of stem cells and the regeneration of dental pulp after transplantation into the adult tooth. MethodsAdipose tissue–derived microvascular fragments (ad-MVFs) were isolated from human adipose tissues. The apoptosis and senescence of DPSCs cultured in conditioned media were evaluated to explore the effects of ad-MVFs on DPSCs. DPSCs combined with ad-MVFs were inserted into the human tooth root segments and implanted subcutaneously into immunodeficient mice. Regenerated pulplike tissues were analyzed by hematoxylin and eosin and immunohistochemistry. The vessels in regenerated tissues were analyzed by Micro-CT and immunofluorescence. ResultsThe isolated ad-MVFs contained endothelial cells and pericytes. ad-MVFs effectively prevented the apoptosis and senescence of the transplanted DPSCs both in vivo and in vitro. Combined with DPSCs, ad-MVFs obviously facilitated the formation of vascular networks in the transplants. DPSCs combined with ad-MVFs formed dental pulp–like tissues with abundant cells and matrix after 4 weeks of implantation. The supplementation of ad-MVFs led to more odontoblastlike cells and increased the formation of mineralized substance around the root canal. ConclusionsCotransplantation with ad-MVFs promotes the angiogenesis and revascularization of transplanted DPSC aggregates, leading to robust regeneration of dental pulp.

Tooth Discoloration Induced by Different Calcium Silicate-Based Cements: A Two-Year Spectrophotometric and Photographic Evaluation in Vitro.

Calcium silicate-based cements (CSCs) may lead to coronal staining in young permanent teeth over the time. The purpose of this study was to evaluate and compare the long-term tooth discoloration induced by different CSCs. Ninety freshly-extracted human molars were assigned randomly into 6 groups (n=15/group) according to the CSC used as a pulpotomy material: ProRoot MTA, MTA Angelus, NeoMTA, EndoSequence Putty, Biodentine and Negative control (No cement). The color was assessed at baseline, and thereafter at 3, 6, 12 and 24 months by using both a spectrophotometer and digital images taken with and without a cross-polarizing filter. The time-dependent changes in color (ΔE) were compared within and among groups using Analysis of Variance. Angelus MTA and ProRooT MTA showed severe coronal discoloration (p>0.05) starting at 3 months. ΔE values of NeoMTA, EndoSequence Bioceramic Putty and Biodentine were below the perceptibility threshold, with Biodentine showing greater ΔE values than NeoMTA and EndoSequence Putty in the absence of statistical significance (p>0.05). Discoloration elicited by CSCs may develop soon after placement, and continue to increase for up to two years. Angelus MTA and ProRooT MTA cannot be recommended for vital pulp therapies in the esthetic zone of young individuals.

Topographic Distribution of Carious Lesion on Young Permanent Mandibular Molars and Its Relation to Periapical Index Score of Apical Periodontitis: A Radiographic Analysis

INTRODUCTION: Caries in young permanent teeth progress rapidly resulting in early pulp involvement which further progresses into loss of mineralized tissue and severe periapical pathologies. The Periapical Index scoring system (PAI) proposed by Ørstavik et al. in 1986 is an invaluable diagnostic tool used to evaluate periapical lesion extent and severity by 2D digital intraoral periapical radiographic analysis with minimal radiation exposure and hence treatment planning.AIM: To evaluate the relationship of the surface distribution of caries in young permanent molars, pulpal involvement and periapical index (PAI) score with respect to individual root forming young permanent mandibular molars. MATERIALS AND METHOD: In this retrospective single-centre study, 100 pulpally involved carious young permanent mandibular molars were included, selected from the departmental records between August 2018-2019. Topographic distribution of caries and radiographic analysis (using PAI) was evaluated to see the effect of anatomical site of caries, individual anatomical form of root, and extent & severity of apical periodontitis developed.RESULT: No significant correlation between the site of caries involvement and PAI score was observed. PAI score for distal roots was higher(PAI> 2) as compared to mesial root for pulpally involved carious young permanent first molars (p-value mesial root-0.576 , distal root-0.591)CONCLUSION: PAI score is independent of the topographic distribution of caries and no correlation was observed. Periapical radiolucency does not depend on caries distribution, either it is mesial, distal or occlusal.

Comparison of different bioactive scaffolds in the treatment of regenerative pulp of young permanent teeth

Comparison of different bioactive scaffolds in the treatment of regenerative pulp of young permanent teeth

An Aberrant Root Development in Severely Extruded Immature Young Permanent Tooth: A Case Report

An Aberrant Root Development in Severely Extruded Immature Young Permanent Tooth: A Case Report

Pulp healing in immature replanted permanent teeth: A competing risk analysis.

Tooth displacement during avulsion causes total rupture of the pulp's neurovascular supply. Revascularization and pulp healing may occur in immature teeth, which gives rise to the recommendation that root canal treatment may not be required. The aim of this study was to evaluate the prognostic factors for the pulp's response after replantation of young permanent teeth. Records from 117 patients with 133 replanted permanent immature teeth were reviewed, and pulp outcomes were classified as healing (hard tissue deposition on the dentinal walls followed by narrowing of the pulp lumen or ingrowth of bone-like tissue inside the pulp canal) or non-healing (pulp necrosis with infection). The effect of clinical and demographic co-variates on the hazards of both outcomes was assessed performing a competing risk model. Pulp necrosis with infection was diagnosed in 78.2% of the teeth, and healing was observed in 12.8% of the teeth. A total of 12 teeth (9.0%) were censored due to prophylactic removal of the pulp or severe external root resorption caused by eruption of adjacent canines. The cs-Cox model demonstrated that the hazards of pulp healing increased in teeth with extra-alveolar periods <15min (csHR: 7.83, 95% CI 1.76-34.80, p=.01), while the hazards of pulp necrosis with infection decreased (csHR: 0.31, 95% CI: 0.10-0.92, p=.04). Teeth replanted with Moorrees' stages 4 and 5 of root development had higher hazards of pulp necrosis with infection than teeth with stage 2 of root development (csHR: 2.23, 95% CI 1.11-4.50, p=.03; csHR: 2.89, 95% CI: 1.40-5.95; p=.01). Pulp healing rarely occurred after replantation of young permanent teeth being associated with short extra-alveolar periods <15min. Early stages of root development decreased the hazards of pulp necrosis with infection.

Remineralizing effect of calcium-sucrose-phosphate with and without fluoride on primary and permanent enamel: Microhardness and quantitative-light-induced-fluorescence™ based in vitro study

To evaluate and compare remineralization potential of commercially available calcium sucrose phosphate (CaSP) (5% EnaFix) individually and with 0.2% Sodium Fluoride (NaF) mouth-rinse (ACT Kids Anticavity Fluoride-Rinse) supplementation through Vickers Microhardness tester (VMH) and Quantitative Light Induced Florescence (QLF™) technology on artificial carious lesions on primary and permanent enamel. In present in-vitro comparative study, artificial carious lesions were created on 60 each, permanent and primary enamel samples followed by pH cycling for a period of 21 days with interventional agents (Control; Distilled water; Test; CaSP alone and CaSP + 0.2% NaF). The samples were evaluated for ΔF values of QLF at, demineralization, 7th, 14th and 21st day and VHN at, demineralization and 21st day of pH cycling. The data were analysed using paired t -test and ANOVA. Intra group comparison showed the statistically significant fluorescence gain with CaSP+0.2%NaF in permanent samples and statistically significant fluorescence gain with both CaSP and CaSP+0.2%NaF in primary samples (p = 0.001). Inter group comparison of interventional agents in both primary and permanent samples showed statistically non-significant variation between CaSP and added NaF group (p = 0.68). Within the limitations of the present study, results showed no significant difference in microhardness improvement and remineralization potential between CaSP and CaSP+0.2%NaF. However, since both showed similar efficacy CaSP looks to be a choice for primary dentition and CaSP with 0.2% NaF can be preferred for mixed dentition with primary and young permanent teeth.

Influence of Isolation Methods on Retention of Pit and Fissure Sealants in Young Permanent Teeth based on Simonsen’s Criteria: A Randomised Clinical Trial

Introduction: Pit and Fissure Sealants (PFS) are chemically-active liquid materials that are introduced into the occlusal surface of caries-susceptible teeth. These form a micro mechanically bonded, protective layer preventing access to caries-producing bacteria, from rest of the oral environment. Aim: To evaluate the retention of PFS placed on young permanent teeth using two isolation methods. Materials and Methods: This clinical study was carried out in the Department of Paediatric and Preventive Dentistry, at Narayana Dental College and Hospital, Nellore, Andhra Pradesh, India. One hundred Permanent First Molars (PFM) from 25 healthy, cooperative children in the age group of 6-9 years were assigned by block randomisation into two groups (isolation type); each group containing the upper and lower PFMs of the right and left side receiving two different treatment modalities (split-mouth design). In Group I (n=50 teeth), the teeth received sealant with rubber dam isolation, and in Group II (n=50 teeth), with cotton roll isolation. The sealant was applied to all the PFMs as indicated. All the teeth were evaluated using Simonsen’s criteria at regular intervals of 3, 6, and 12 months. The data collected were subjected to statistical analysis using the SPSS statistical package (version 17) and statistical significance was set at 0.05. The difference in the retention of PFS with the two isolation methods was analysed using the Chi-square test. Results: After 12 months, the marginal integrity rate for sealants placed with a rubber dam and cotton roll isolation were 75% and 64%, respectively. The results were better in Group I than in Group II, although there were no statistically significant differences with respect to marginal integrity, discolouration, and anatomical failure. Conclusion: Retention of PFS in young permanent teeth was better with rubber dam than cotton rolls for isolation, though the results were not statistically significant.

Evaluation of Embrace WetBond and Helioseal-F sealant retention with and without a Self-etch adhesive: A 12 month follow-up.

BackgroundPit and fissures on the young permanent tooth are ideal in harbouring dental plaque and calculus. Hence it is important to provide a preventive agent to protect against dental caries. Aim: To evaluate the retention of two different pit and fissure sealants with and without a self-etch adhesive in the first permanent molars for a period of one year. Material and Methods280 molars were included among 70 healthy children. According to randomisation, the groups were divided into Group IA - Embrace without Adhse One F bonding agent and Group IB - Embrace with Adhse One F bonding agent, Group IIA - Helioseal F without Adhse One F bonding agent and Group IIB - Helioseal F with Adhse One F bonding agent. The sealants were assessed clinically at 3,6,9, and 12 months using the modified Colour, Coverage, Caries (CCC) sealant evaluation system. ResultsAt the end of 12 months, the retention rates of Group IB showed statistically significant results, followed by Group IIB, Group IA and Group IIA. ConclusionsAt twelve months follow-up Embrase WetBond and Helioseal-F were better retentive when used with a Adhese One F bonding agent which was statistically significant. Key words:Retention, Bonding agent, Embrace WetBond, Helioseal-F.

Pulp canal obliteration after extrusive and lateral luxation in young permanent teeth: A scoping review.

In the literature, no single therapeutic approach is preferred for treating dental pulp responses such as pulp canal obliteration (PCO). Some authors still recommend preventive endodontic treatment, whereas others choose not to intervene, to avoid causing possible iatrogenic complications. This review was conducted to explore, in the current scientific literature, the degree of knowledge concerning the onset and development of PCO and pulp necrosis (PN) following dental trauma (i.e. extrusive luxation and lateral luxation) in children and young adults (6-20 years). The authors also evaluated whether and to what extent the stage of dental root development at the time of traumatic dental injury (TDI) influences these pulp responses, and whether PN can arise in teeth already affected by PCO. MATERIALS AND METHODS The literature search was carried out during the period October 2019 to January 2020, using the following databases: PubMed/MEDLINE, SCOPUS and Web of Science. Articles in English reporting on young patients with PCO or PN in permanent teeth affected by trauma were included. Theresearch, whose questions were summarised according to the PICO method, considered the following aspects: patient age, type of TDI, stage of root development, initial treatment, presence of calcification or necrosis, time to onset of the two outcomes (PCO and PN), and duration of follow-up. The articles found were evaluated by two reviewers; in the event of disagreement regarding the inclusion of an article, a third reviewer was called upon to decide. The initial screening of the databases, using the selected search keywords, yielded a total of 343 articles. After exclusion of duplicates and articles not meeting the inclusion criteria, 11 articles remained. Of these, only four completely met the inclusion criteria. Closer analysis of these four publications revealed that they would not easily yield standardised sets of clinical data that might be homogenised in order to produce clear meta-analytical data. Hence the need to limit the data collected to the following seven items: number and type of injuries, type of initial intervention, duration of follow-up, main pulp responses, number and type of pulp responses. PCO is a physiological pulp response associated with a traumatic event, usually a luxation injury. It is diagnosed on the basis of combined radiographic and clinical-anamnesticdata. Signs of PCO start to appear at around one year after the traumatic event, and its development reaches completion by about five years post-trauma. PN, on the other hand, is clearly apparent within the first year. Endodontic treatment, be it carried out as a preventive measure or following detection of PCO, is inappropriate and can cause serious iatrogenic damage. Treatment is indicated only in cases of definite PN.

Effect of Enamel Pre-etching with Sodium Hypochlorite Deproteinisation and Bonding Agent on Retention and Microleakage of Pit and Fissure Sealants: An In-vitro Study

Introduction: Sealants have proved to be one of the easiest methods of caries prevention in young permanent teeth, the placement of which is very technique sensitive. The non-invasive method of pretreating the enamel surface by deproteinising it with 5.25% sodium hypochlorite (NaOCl) for 60 seconds prior to etching has proven to be a promising method for improving retention and reducing microleakage of sealant. Aim: To evaluate the effect of enamel pre-etching with sodium hypochlorite deproteinisation and bonding agent on retention and microleakage of pit and fissure sealants. Materials and Methods: This in-vitro experimental study was conducted in Department of Paediatric and Preventive Dentistry at Annoor Dental College, Muvattupuzha, Kerala and Nanotechnology Lab at Amrita Institute of Medical Science. Freshly extracted Intact permanent maxillary and mandibular 20 third molars and 20 premolars mounted on acrylic blocks were divided into 4 groups containing 5 teeth in each group. Molars and premolar were taken for assessing the rate of retention and microleakage, respectively. Teeth in Group 1 were subjected to acid etching only, Group 2 were deproteinised prior to acid etching, Group 3 were subjected to acid etching followed by bonding and Group 4 were subjected to deproteinisation, etching and bonding. Then, all teeth were sealed with pit and fissure sealant and underwent thermocycling in artificial saliva bath for evaluation of sealant retention and microleakage. After thermocycling, retention was assessed on molar by one pre-calibrated blinded examiner by passing a 0.5 mm diameter probe along the margins of the sealant placed to verify integrity, failure, or loss of continuity based on Simonsen’s criteria (1989). To assess microleakage, premolar samples were immersed in rhodamine B, sectioned longitudinally, and examined under a stereomicroscope for assessment of microleakage. Collected data were statistically analysed using Chi-square and Mann-Whitney u test. Results: The results showed 100% retention for teeth in Group 2a, Group 3a, Group 4a, while partial sealant loss was present in 3 teeth from Group 1a. The differences between the groups for retention was statistically significant (p=0.014). In the case of microleakage, Group 3b showed more microleakage and group 4b showed least microleakage and the difference between them was statistically significant (p=0.009). Conclusion: Deproteinisation and use of bonding agent increases retention of the pit and fissure sealants. However, deproteinisation is a more effective method to control microleakage when compared to bonding agent. Thus, it can be considered as an effective method for pretreating enamel surface before sealant placement.

Development and Application of a Quick Cleaning and Portable Storage Device for Dislocated Teeth

Background: The teeth with dental trauma in children are mostly young permanent teeth of school-age children. Once trauma occurs, it will have adverse effects on children's masticatory function, occlusal relationship, beauty, growth and development. Objective: To improve the success rate of replantation of completely dislocated teeth. Methods: Through literature review, we focus on “Tooth Injuries, Complete Dislocation, Replantation” to understand the current rescue measures of dislocated teeth, and conceive a rapid cleaning and portable storage device for dislocated teeth. Results: A device for rapid cleaning and portable preservation of dislocated teeth was successfully designed. Setting the ultraviolet sterilization lamp can play the role of irradiation sterilization and setting the mesh vertical plate can prevent the violent collision of multiple teeth when cleaning. It is possible to divide the storage slot space by setting the baffle, so as to achieve the purpose of classified storage. Conclusion: A rapid cleaning and portable storage device for dropped teeth is described, which solves the problems that the existing rapid cleaning and portable storage device cannot clean teeth uniformly, the cleaning effect is poor, and the cleaned teeth cannot be stored at low temperature.

Conservative management of traumatized young permanent tooth: A 1-year follow-up

Traumatic exposure of pulp in immature permanent teeth is most commonly observed in pediatric patients. Preserving the dental pulp is an important factor while treating an incompletely formed root of a traumatized tooth. Cvek pulpotomy is a method which helps in maintaining its vitality by providing a favorable environment for continued root development. This report describes a case of a young patient whose maxillary right central incisor suffered crown fractures because of a traumatic accident in school. Cvek pulpotomy with mineral trioxide aggregate was performed in order to achieve apexogenesis, and the tooth was restored with glass ionomer cement and the tooth fragment was reattached. The patient was recalled at 1, 3, 6, and 12 months.

Clinical Observation of the Effect of Erbium-Doped Yttrium Aluminium Garnet Laser on the Treatment of Pit and Fissure in Children

To apply erbium-doped yttrium aluminium garnet laser to the closure of young permanent teeth and pits, compare the total retention rate of sealants, and discuss whether erbium-doped yttrium aluminium garnet laser is suitable for clinical treatment in children. Sixty children with 6-9 y old and bilateral mandibular first molars with complicated sulcus were randomly divided into two groups: A and B. The self-half control method was used: group A children’s control side test teeth application of corundum The enamel was performed by enamel, acid etching, coating pit and fissure sealant, erbium-doped yttrium aluminium garnet laser enameloplasty was applied to the contralateral test teeth and pit and fissure sealant was applied; group B children’s control side test teeth were treated with group A. The contralateral test teeth were treated with erbium-doped yttrium aluminium garnet laser enameloplasty, acid etching, and coating pit and fissure sealant. The children were scored for treatment after surgery, and regular follow-up was performed to observe the retention of the sealant. 63.3 % of the children tested were willing to take the initiative to choose laser treatment. At the 3rd and 6th mo of follow-up, there was no significant difference in the total retention rate of the two groups of sealants (p>0.05); at the 12th and 18th mos of followup, the total retention rate of the sealant materials: laser+etchant group>traditional bur. The difference between the needle group and the laser group was statistically significant (p<0.05). Laser is a more acceptable treatment for children. Erbium-doped yttrium aluminium garnet laser is used in the formation of permanent enamel. It needs to be combined with acid etchant to enhance the retention of the sealant.

Conceptual combination of disinfection in regenerative endodontics: Conventional versus laser-assisted disinfection.

Background:Successful regeneration depends on four key elements of tissue engineering such as stem cells, growth factors, scaffold and sterile environment. Therefore, regenerative endodontic therapy requires higher degree of disinfection for successful outcomes.Aims and Objective:To evaluate the clinical and radiographic outcome using conventional and laser assisted disinfection in regenerative endodontics at 1, 3 and 6 months.Materials and Methods:18 children with necrotic young permanent teeth were selected for the study. In Group A, in the first appointment AAE (American Association of Endodontics, Clinical Consideration for Regenerative Endodontic Procedure - 2016) disinfection protocol was employed. In Group B laser assisted disinfection (810 nm diode laser, 1 W, 20 ms Pulse length and 20 ms interval duration) was performed. Microbial samples were obtained before and after disinfection to check the level of disinfection. The cases were followed up at 1 month, 3 months and 6 months interval and evaluated for clinical outcome, periapical healing and apical response. Data were statistically analyzed with level of significance set 1% or 5% accordingly (P<0.01 or P<0.05)Results:On intragroup comparison there was significant reduction in bacterial count before and after disinfection with respect to laser assisted disinfection group. (P value = 0.007) There was no significant change in the clinical outcome score in laser group at 1,3- and 6-months interval. Periapical healing score showed statistically significant results in laser group when followed from 3 months to 6 months (P value = 0.04).Conclusion:Along with AAE 2016 protocol, use of laser assisted disinfection resulted in improved quality of disinfection and an expeditious clinical outcome and periapical healing in necrotic young permanent teeth. Keywords: Disinfection; laser; regenerative endodontics; sodium hypochlorite; tooth apex