Coconut Water Research Articles

A super convenient and specific CRISPR/Cas12a diagnostic platform for toxigenic Burkholderia gladioli based on virulence genes

In recent years, food poisoning cases caused by toxigenic Burkholderia gladioli pv. cocovenenans (BGC) have been frequently reported in Asian countries like China and Indonesia, which seriously threaten food safety and public health. BGC is the only subspecies within the Burkholderia gladioli species that can cause food poisoning in humans, but there is a high degree of sequence homology between the different subspecies, making detection and differentiation difficult. In this study, a specific DNA target was screened out from the bongkrekic acid biosynthentic gene cluster. A rapid, sensitive, and highly specific CRISPR/Cas12a molecular diagnostic platform was developed for the first time to differentiate between toxigenic and non-toxigenic subspecies. Genomic DNA was extracted from the samples after a simple boiling process. The sensitivity was evaluated from three different levels, the genomic DNA sensitivity was determined to be 10−4 ng/μL, the target DNA sensitivity was determined to be 14.9 copies/μL, and the bacterial suspension sensitivity was confirmed to be 1.52 CFU/mL. The detection limit of BGC in artificially contaminated coconut water samples was determined to be 1.50 CFU/mL without any microbial enrichment. Including sample pretreatment and DNA extraction process, the whole detection of BGC in samples can be completed within 60 min under the assistance of some small and portable devices. This super convenient diagnostic platform is suitable for food field testing and provides a theoretical basis for the specific identification of BGC.

Evaluating the Probiotic Profile, Antioxidant Properties, and Safety of Indigenous Lactobacillus spp. Inhabiting Fermented Green Tender Coconut Water

Evaluating the Probiotic Profile, Antioxidant Properties, and Safety of Indigenous Lactobacillus spp. Inhabiting Fermented Green Tender Coconut Water

Eco-friendly production of leather-like material from bacterial cellulose and waste resources

In recent years, resource optimization has been recognized as a top priority among many challenges. Studies have indicated that valuable products can be created by combining natural or discarded resources with cutting-edge biosynthesis techniques. This approach is considered foundational for establishing a “zero-waste” socio-economic structure. This research focuses on optimizing resources and minimizing environmental impacts, especially within the manufacturing and fashion sectors. An innovative leather-like material named BC-BioLeath (Bacterial Cellulose-Based Bio Leather) has been introduced. BC was produced by the fermentation of coconut water, a byproduct of the food industry. Traditional leather-like processing, including tanning, was applied to BC using food waste such as oxidized edible oil and coffee grounds, along with timber industry by-products like vegetable tannins. Studies involving SEM observation, mechanical, style, and dyeing performance testing revealed that the product, BC-BioLeath, exhibited superior mechanical properties. For instance, BC crosslinked with oxidized edible oil showed a tensile strength of 82.14 MPa, surpassing traditional cowhide used in shoe uppers by 2.08 times. When plasticized with glycerol, the tensile strength reached 56.19 MPa, with breaking elongation of 36%. Beyond its mechanical prowess, the product also achieved economic and environmental benefits through waste repurposing, contributing to a natural energy cycle. BC-BioLeath was identified as a more humane and eco-friendly leather alternative. This method showcased not just efficient waste repurposing but also introduced a novel, microbe-focused sustainable production approach. Such a model offered a genuine circular economy solution for high-polluting sectors, such as the leather fashion industry. A cost analysis showed that BC-BioLeath's production cost of $97.92/m2 (excluding labor and operating costs: $7.52/m2) was within the commercial leather range of $39.7 to $220.8/m2. This indicated its sustainability as a competitive alternative.

Activated biochar production from young coconut waste (Cocos nucifera) as bioadsorbent: a pathway through Artificial Neural Network (ANN) optimization.

This pioneering work explores the immense potential of young coconut waste, a continuously marginalized residue of the food and beverage industry, to serve as an indispensable feedstock in the production of biochar. Through an examination of the key carbonization factors that include time, temperature, and concentrations of the activating agent, KOH, the outcomes offer relevant insights that could be leveraged to maximize biochar production for tailored applications. This study stands out for its innovative use of Artificial Neural Network (ANN) approaches for predictive modeling. Fifty datasets, supplemented with secondary data obtained from the literature and experiments, were utilized for the purposes of training, testing, and validating the neural network model. Here, the datasets were processed utilizing the Deep Neural Network (DNN) framework, which was designed and implemented with the minimal loss function framework feasible. The architectural configuration comprises the following; an input layer, four hidden layers (128-neuron dense layer, batch normalization, and 64-neuron dense layer, batch normalization), a dropout layer, and an output layer. With an R2 of 0.8238 for biochar yield and 0.7324 for iodine number, the trained DNN model showed a relatively high degree of accuracy in making predictions.

Characterization of Bacterial Nanocellulose Produced by Tropical Fruit‐Derived Bacteria in Coconut Water Medium Supplemented with Pineapple Peel Extract

AbstractAgro‐industrial residues, such as surplus fruits and vegetables are increasingly recognized as valuable sources for isolating bacterial strains capable of efficiently producing bacterial nanocellulose (BNC). This study presents a novel approach to BNC production using Komagataeibacter intermedius SB 110 isolated from rotten pineapple, cultivated in a sustainable medium of coconut water enriched with 5% sucrose and 0.5% pineapple peel extract. By leveraging agricultural waste, this method accelerates BNC biosynthesis to less than 7 days and achieves a yield of 5.563 g/L over 14 days, a 1.58‐fold increase compared with traditional media. The BNCs were primarily composed of cellulose type I, with minor cellulose type II, ultrafine fiber sizes (58.3 to 84.98 nm) and a high crystallinity index (60.81% to 78.34%), indicating superior structural quality. This innovative process not only demonstrates the potential of using low‐cost, sustainable substrates for high‐yield BNC production but also significantly enhances both the efficiency and scalability of the production process, setting a new standard over existing methods.

Microbiological Quality of Coconut Water Sold in the Grande Vitória Region, Brazil, and Phenogenotypic Antimicrobial Resistance of Associated Enterobacteria.

This study aimed to evaluate the microbiological quality of coconut water sold from street carts equipped with cooling coils or refrigerated at bakeries in the Grande Vitória Region, Brazil. Additionally, it assessed the phenotypic and genotypic antimicrobial resistance profiles of isolated enterobacteria. The results indicated that coconut water sold at street carts had lower microbiological quality compared to refrigerated samples, as evidenced by significantly higher counts of mesophilic microorganisms. Using MALDI-TOF, the following opportunistic pathogens were identified: Citrobacter freundii, Enterobacter bugandensis, E. kobei, E. roggenkampii, Klebsiella pneumoniae, and Kluyvera ascorbata. Three isolates-E. bugandensis, K. pneumoniae, and K. ascorbata-were classified as multidrug-resistant (MDR). Widespread resistance to β-lactams and cephalosporins was detected, and some isolates were resistant to quinolones, nitrofurans, and phosphonic acids. The gene blaCTX-M-2 was detected in C. freundii, E. bugandensis, E. kobei, and K. ascorbata. However, genes blaNDM, blaKPC, blaCMY-1, and blaCMY-2 were not detected in any isolate. The findings underscore the need to enhance good manufacturing practices in this sector to control the spread of antimicrobial resistance (AMR). To our knowledge, this is the first study documenting the presence of potentially pathogenic enterobacteria in coconut water samples and their associated phenotypic and genotypic AMR profiles.

Whole-Genome Resequencing Identifies SNPs in Sucrose Synthase and Sugar Transporter Genes Associated with Sweetness in Coconut.

Coconut (Cocos nucifera L.) is an important agricultural commodity with substantial economic and nutritional value, widely used for various products, including coconut water. The sweetness is an important quality trait of coconut water, which is influenced by genetic and environmental factors. In this study, we utilized next-generation sequencing to identify genetic variations in the coconut genome associated with the sweetness of coconut water. Whole-genome resequencing of 49 coconut accessions, including diverse germplasm and an F2 population of 81 individuals, revealed ~27 M SNPs and ~1.5 M InDels. Sugar content measured by °Bx was highly variable across all accessions tested, with dwarf varieties generally sweeter. A comprehensive analysis of the sugar profiles revealed that sucrose was the major sugar contributing to sweetness. Allele mining of the 148 genes involved in sugar metabolism and transport and genotype-phenotype association tests revealed two significant SNPs in the hexose carrier protein (Cnu01G018720) and sucrose synthase (Cnu09G011120) genes associated with the higher sugar content in both the germplasm and F2 populations. This research provides valuable insights into the genetic basis of coconut sweetness and offers molecular markers for breeding programs aimed at improving coconut water quality. The identified variants can improve the selection process in breeding high-quality sweet coconut varieties and thus support the economic sustainability of coconut cultivation.

An Analysis Of Energy And Nutrient Content In High Energy Natural Isotonic Drink (Isopower)

Isotonic drink is the carbonated or non-carbonated drink product used to subtitute the fluid lost in the body. Lack of fluid in the body can cause muscle fatigue until causing the performance decrease in athlete. Isotonic drink can accelerate the return of body fluid compared to water. The purpose of this research was to analyze the content of energy, sodium, potassium, and Vitamin C in isotonic drink with raw material coconut water, pure coconut oil, bee bread, palm vinegar, and honey as high energy natural istotonic drink. The research design used was descriptive observation based laboratory test. The data analysis employed SPSS 22 programs namely by carrying out descriptive test until obtained the average for each isotonic namely 247,491 kkla, sodium 77,085 mg.kg, potassium 1000,785 mg/kg and Vitamin C 2,112 mg. This research concluded that the energy drink content has fulfilled the quality specification of isotonic drink, while the sodium and potassium level do not fulfill the quality specification of isotonic drink yet. Likewise with the Vitamin C content which also does not fulfill the Referrence of Nutrition Label namely 10%.

Economic Empowerment of Residents of Al-Hidayah Kalumbuk Padang Orphanage by Utilizing Virgin Coconut Oil Manufacturing Waste into Nata de Coco Beverage Products as Additional Income

The "Al-Hidayah" orphanage, established in Kalumbuk, Padang City, in 1987, by the Al-Hidayah Foundation. The establishment of this orphanage is because in the Kalumbuk area there are many orphans and orphans, who are economically deprived. They struggle with the cost of living and the cost of education. The number of them at the beginning of the establishment was 35 men, and 15 women. Those who live in the orphanage are boys, while women live with their parents, in houses around the orphanage. The current economic situation of the orphanage is still in shortage, because it still depends on government assistance and donations from the community. Meanwhile, orphanage children have potential, especially high school and junior high school students who number 26 people. If directed to fill their free time with activities that can generate additional income, it will help the economy of the orphanage. Previously, the children of this orphanage had been trained, how to make a VCO by the service team chaired by Prof. Dr. Suryani MSi, at their own expense. In the process of making VCO, the waste, which is in the form of coconut water, is wasted. Partners do not have knowledge about the use of coconut water waste into Nata de coco, as well as used as food and beverages that can be sold and make money. The purpose of the service is to utilize the waste produced by VCO, namely coconut water into Nata de coco and its drinks, to improve the economy of the orphanage. This service program is carried out through training in making Nata de Coco from coconut water and making drinks from Nata de Coco. As a result, Mitra has marketed its products, by serving orders for the procurement of drinks from Nata de Coco at the Wedding Party. So that it has generated an average monthly income of Rp. 6,750,000. This program has succeeded in increasing Partners' income

Consumer preference on packed tender coconut water: Conjoint analysis approach

The study examined consumer preferences towards packed tender coconut water, which has witnessed higher demand in recent times. This is mainly due to its potential health benefits and being an ideal alternative to energy drinks and other carbonated beverages. For this study, primary data were collected from 150 consumers in Coimbatore using a well-structured questionnaire. Expending conjoint analysis, it was found that brand name was recorded as a highly preferred attribute, followed by quality parameters and price range. Therefore, creating awareness about the health benefits using various promotional tools and increasing its availability by establishing tender coconut water kiosks would raise its consumption. Further, due to the emergence of digital marketing adoptions, firms could focus on its availability through online platforms, which could increase consumption. Therefore, processing firms, in addition to traditional channels, should focus on adopting digital marketing channels with innovative products that have natural taste and good quality. Keywords: Brand, consumer buying behaviour, online platforms, processed foods, quality. JEL Codes: L66, M31.

Assessment of Calcium Concentration and pH at the Periapical Environment on Placing Calcium Hydroxide with Different Natural Vehicles – An In vitro Study

Abstract Background: In root canal therapy, the number of microorganisms in canals can be controlled by giving proper intracanal medication dressing. Calcium concentration and changes in pH at the periapical region denote the effect of antibacterial action in the periapical region. Aim: The aim is to determine a suitable natural vehicle for calcium hydroxide (Ca(OH)2) as an intracanal medicament by assessing the calcium concentration and changes in pH at the periapical region. Materials and Methods: A total of 60 extracted single-rooted permanent teeth were equally allotted into four groups – Group I: Ca(OH)2 with distilled water, Group II: Ca(OH)2 with aloe vera, Group III: Ca(OH)2 with coconut water, and Group IV: Ca(OH)2 with neem extract. pH was determined by pH meter and calcium by chromatography. The values were taken on days 1, 2, 3, 7 and 14. Results: All the materials showed alkaline pH and release of calcium ion throughout the study. For day 1, the mean pH value was highest for distilled water (10.06) and lowest for aloe vera extract (9.42). The highest calcium release during day 1 was by coconut water (67.85) and the least calcium release was by aloe vera (42.01). On day 14, the highest calcium release was by distilled water (89.98) followed by coconut water (67.24) and least by neem extract (61.13). pH values and calcium release in four different natural vehicles were significant (P < 0.05). Conclusion: All natural materials used in this study showed good Ca(OH)2 diffusion when used as a vehicle with Ca(OH)2 in root canals.

Effect of sodium metabisulfite treatment and storage condition on metabolic profile of young coconut (Cocos nucifera L.)

Young coconuts (Cocos nucifera L.) used for export are trimmed to reduce their size and weight to lower transport costs. However, trimmed coconuts have a shorter shelf life due to microbial spoilage and surface discoloration caused by enzymatic browning. To minimize these effects, trimmed coconuts were dipped in an anti-browning agent, sodium metabisulfite (SMB), and stored under ambient conditions. However, there have been no reports on the effects of SMB treatment on metabolome changes in the flesh and water of young coconuts. Hence, this study investigated the metabolite changes in trimmed young coconuts after SMB treatment under different storage conditions using a gas chromatography (GC)/mass spectrometry (MS) metabolomic profiling approach. Tall young coconut samples were trimmed and treated with a 2% SMB solution for 5min before storage at 25°C or 4°C for 2-4 weeks. Coconut flesh and water samples were collected after storage for 0, 2, and 4 weeks, and were subjected to GC-MS analysis. The results showed that the major metabolites affected by coconut deterioration were amino acids, sugars, and sugar alcohols. SMB treatment and/or refrigeration can help prevent metabolite changes in the flesh and water of young coconuts. In the future, improvements in storage conditions based on metabolite profiles should be explored.

Supercritical carbon dioxide–activated copper/bacterial cellulose aerogels for the capture of hydrophobic organic liquids

In this study, bacterial cellulose was derived from coconut water via fermentative production of nata de coco, a common food in Southeast Asia. Bacterial cellulose offers distinct advantages over plant cellulose resources, including three-dimensional structure, high porosity, high cellulose purity, and the absence of required polluting treatments, making it an ideal candidate for the facile preparation of various functional aerogel materials. Surface modification of nata de coco-derived cellulose fibers was obtained by incorporating Cu species (approximately 15 wt%) through an aqueous reaction of Cu(CH3COO)2 with N2H4 at room temperature. The Cu-coated bacterial cellulose aerogels were fabricated via supercritical carbon dioxide drying to maintain the natural three-dimensional matrix and subsequently characterized by various techniques confirming high porosity and crystallinity and successful Cu coating onto the cellulose fiber surface. The Cu-containing aerogel showed a significantly improved adsorption uptake for n-hexane (12.6 g g−1) in comparison with pure bacterial cellulose–based aerogel (3.2 g g−1). Furthermore, notable adsorption performances of 13.6–19.9 g g−1 were found for cyclohexane, ethyl acetate, and soybean oil, indicating the high efficiency of the supercritical carbon dioxide–activated bacterial cellulose aerogel containing Cu species in the adsorption of water-insoluble liquids. Notably, applying supercritical carbon dioxide drying afforded the stable aerogel structure for reusing over several cycles with almost unchanged trapping capacity.

Coconut water combined with purebred chicken egg yolk as an alternative semen extender for Madura Pote bucks semen preservation

One of the many efforts to increase the quality of livestock genetics is through artificial insemination (AI). Other than increasing it, AI can be conducted to preserve semen. A successful AI is determined by seminal quality, therefore, a method to preserve semen for a longer storage time is needed. The method used is adding an extender that fulfills prerequisites for a semen extender such as coconut water combined with egg yolk citrate extender. Coconut water is rich in carbohydrates, protein, vitamins, and antioxidants while egg yolk contains lecithin. This study aims to find out the Pote buck spermatozoa quality stored in coconut water and egg yolk extender. This study uses three groups of treatments (T0: 0.1 ml semen + 0.9 ml egg yolk citrate, T1: 0.1 ml semen + 0.9 ml coconut water, and T2: 0.1 ml semen + egg yolk citrate (20%) + coconut water). All three of these are stored at 5oC and evaluated every day until day 5 of their motility, viability, intact plasma membrane, abnormality, and MDA level. Data analysis used is ANOVA and a further test called BNT is conducted if a significant difference is determined. No significant difference was found between T0 and T1 (p>0.05). The highest progressive motility, viability, and intact plasma membrane (%) among the three groups of treatments happened to be from T2. Meanwhile, a low percentage of spermatozoa abnormality and MDA level were also found in T2 with its extender being coconut water combined with egg yolk citrate. To conclude, the best extender for storing Pote buck semen is stored at 5oC is coconut water combined with egg yolk citrate extender.

Pengaruh Air Kelapa, Ekstrak Bawang Merah dan Ekstrak Rebung Bambu Terhadap Perkecambahan Benih Kopi Robusta (Coffea canephora)

Background: Robusta (Coffea canephora) is one type of coffee widely cultivated in Indonesia and is one of the leading commodities. Coffee seeds include seeds that have a long dormancy period that inhibits the germination process. Breaking dormancy can be done by several methods, one of which is soaking in natural growth regulators. Soaking treatment with natural growth regulators can be combined with soaking time treatment to break dormancy. This study aimed to determine the combination of natural growth regulator treatment and the right soaking time for Robusta coffee seed germination. Methods: This research is a 3-repeat Complete Factorial Randomized Design Experiment with the first factor of natural growth regulators type, which consists of 4 levels, namely: Z0 = control; Z1 = coconut water; Z2 = onion extract and Z3 = bamboo shoots, and the second factor is the length of soaking which consists of 3 levels, namely P1 = 12 hours; P2 = 24 hours and P3 = 36 hours. Results: Natural growth regulators' treatment of shallot extract with 24 hours of soaking time gave the best results on all parameters. Conclusions: Using natural growth regulators from coconut water, shallots, and bamboo shoots with several soaking times affects all parameters observed, both in single and combined administration.

Application of Bacillus sp Liquid Formula Using Root Infusion Technique To Control VSD (Vascular Streak Dieback) Disease In Cocoa

This study aims to explore the potential of liquid organic waste formula made from Bacillus sp to suppress the severity of vascular streak dieback (VSD) disease in cocoa plants. The research was conducted on smallholder cocoa plantations in Patimbe Village, Palolo Subdistrict, Sigi Regency, Central Sulawesi. The research was arranged in a non-factorial Randomised Group Design (RAK) with 5 treatments and 5 replications. The treatments consisted of control (P0=no formula); coconut water + Bacillus sp (P1); rice washing water + Bacillus sp (P2); tofu water + Bacillus sp (P3); and a combination of coconut water, rice washing water, and tofu water + Bacillus sp (P4). Each experimental unit consisted of 10 cocoa plants infected with VSD disease. The liquid organic waste formula was applied using the root infusion technique in months 1 and 3 at a dose of 250 ml/root. Observations of disease symptoms, disease development, disease severity, and disease suppression efficacy were conducted monthly. The results showed that the combination of organic waste of coconut water, tofu water, and rice washing water with Bacillus sp active ingredient was more effective in suppressing VSD disease than liquid organic waste alone with an effectiveness rate of 81.7%.

PHARMACEUTICAL STUDY OF ERANDA TAILA EXTRACTED FROM ERANDA BEEJA WITH AND WITHOUT SHODHANA

Eranda Taila (castor oil) is a widely used drug in Ayurveda. The classical texts Yogaratnakara and Rasatarangini have mentioned the shodhana process (purificatory procedure) of Eranda beeja (Ricinus communis Linn. seeds). Shodhana aims to nullify or reduce toxins and improve the therapeutic efficacy and potency of a drug. The shodhana of Eranda beeja (castor seeds) was performed as per the classics, using the method of swedana, which involves heating a pottali (cloth pouch) containing decorticated Eranda beeja immersed in narikelodaka (fresh tender coconut water) for 3 hours. Eranda Taila was extracted from shodhita (purified) and ashodhita (unpurified) Eranda beeja using the traditional method of castor oil extraction. The obtained shodhita Eranda Taila was light brown, had the characteristic odour of coconut and castor, was less nauseating, and was yielded in higher quantity than ashodhita Eranda Taila. The ashodhita Eranda Taila was creamish yellow and exhibited the characteristic odour of castor.

Effect of Sperm Extender and Dilution Ratio on The Sperm Motility, Fertility, and Hatching Rates of Depik Fish Rasbora Tawarensis (Pisces: Cyprinidae) Eggs

Abstract There is no known artificial breeding method for depik fish Rasbora tawarensis. Sperm extender and the dilution ratio are crucial factors in the artificial breeding of fish. The current study aimed to assess the effect of several common fish sperm extenders and its dilution ratio on sperm characteristics and fertilization of depik fish eggs. Two series of experiments were performed to find the best extender and its dilution ratio. In the first experiment, five types of extenders were tested: Ringer’s solution, physiological solution, coconut water, sugarcane water, and roomie water. The second experiment studied four dilution levels of sperm and extender (v/v): 1:20, 1:30, 1:40, and 1:50. Every treatment was conducted in four replicates and the experiments were conducted consequently so that the findings in the first experiment were applied in the second. The first experiment showed that Ringer’s is the best extender for depik sperm with sperm motility, fertilization and hatching rates of 71.00%, 69.30%, and 53.66%, respectively; and the second experiment revealed that Ringer’s at dilution ratio 1:40 gave higher sperm motility, fertilization, and hatching rates of 74.66, 70.33, and 59.00%, respectively. In conclusion, Ringer’s solution was the most favorable extender for sperm which yielded best fertilization results at 1:40 dilution ratio (sperm:extender, v/v) in depik fish.

Silver Core Coated with Molecularly Imprinted Polymer as Adsorbent in Pipet-Tip Solid Phase Extraction for Neonicotinoids Determination from Coconut Water.

In this work, we report an innovative adsorbent named Ag-MPS@MIP that has a core@shell structure, i.e., silver nanoparticles modified with 3-methacryloxypropyltrimethoxysilane as the core and molecularly imprinted polymer based on methacrylic acid as its shell. Thiamethoxam, imidacloprid, and acetamiprid were extracted from coconut water samples using Ag-MPS@MIP in pipet-tip solid phase, prior to high-performance liquid chromatography analysis. The separation was carried out on isocratic mode using a mobile phase consisting of C18 column (Phenomenex, 150 mm × 4.6 mm, 5 μm), ultrapure water acidified with 0.3% phosphoric acid:acetonitrile (78:22, v/v), flow rate at 1.0 mL min-1, injection volume of 10 μL, temperature of 25 °C, and wavelength at 260 nm. The adsorbent and precursor materials were properly characterized by different instrumental techniques. The main factors affecting the recovery of analytes from coconut water samples by pipet-tip solid phase were optimized, such as sample volume (250 μL), sample pH (pH = 5.0), ionic strength (1%, m/v), washing solvent (300 μL ultrapure water), volume and type of eluent (500 μL methanol), amount of adsorbent (15 mg), cycle of percolation-dispensing (1×), and reuse (5×). Thereby, the neonicotinoids presented extraction recoveries between 82.80 and 96.36%, enrichment factor of 5, linearity ranged from 15 to 4000 ng mL-1, correlation coefficient (r) > 0.99, limit of detection of 5 ng mL-1, satisfactory selectivity, stability, and proper precision (RSD%: 0.52-9.64%) and accuracy (RE%: -5.19-6.45%). The method was successfully applied to real samples of coconut water.

Pengelolaan limbah rumah kemas sayur melalui produksi pupuk organik cair

Background: The growth of the agricultural sector in Cipanas District, Cianjur Regency, West Java has increased the production of organic waste from vegetable packaging houses. This study aims to explore the method of converting vegetable waste into Liquid Organic Fertilizer (POC) for waste management and sustainable agriculture. Methods: This descriptive study evaluated the process of converting vegetable waste into POC in Cipanas District. The process includes soaking rice grits, mixing ingredients (rice soaking water, brown sugar solution, coconut water, vegetable waste), anaerobic fermentation for three weeks with periodic stirring, and harvesting. Findings: The study resulted in a description of the process of making POC from vegetable waste. POC has the potential to improve soil fertility holistically (physically, chemically, biologically) and has been proven effective in increasing the productivity of various types of vegetables. Conclusion: Utilization of vegetable waste as POC offers a sustainable solution for waste management and environmentally friendly agriculture. Although there are challenges in quality standardization and infrastructure, its economic potential and long-term benefits make this approach worthy of consideration in future agricultural strategies. Novelty: This study highlights the potential use of vegetable waste from packaging houses in Cipanas for POC production, integrating the concept of circular economy in agricultural waste management and the development of sustainable agricultural practices.