Yolk Powder Research Articles

Subcritical fluid extraction treatment on egg yolk: Product characterization

In this study, the method of subcritical fluid-propane extraction (SPE) used to coproduce egg yolk oil (EYO) and low-fat egg yolk powder from spray-dried egg yolk powder (EYP) was investigated. Results showed that the optimum parameters were: extraction temperature of 313.15 K, extraction time of 120 min, and solid-liquid ratio of 1:9 (g/mL). Under this condition, the residual oil rate of EYP was 21.04%, which was much lower than that of ethanol extraction (39.24%). Scanning electron microscopy reveled that serious disruptions of egg yolk sphere extracted by SPE were observed, facilitating the EYO extraction. In addition, oil extracted by SPE had lower acid value and peroxide value than that of ethanol extraction. Moreover, the emulsifying properties and solubility of low-fat EYP obtained by SPE were similar to the original egg yolk powder. This study provides a new technique for coproducing high-quality oil and protein.

Variation in the structure and emulsification of egg yolk high-density lipoprotein by lipid peroxide.

To further clarify the effect of the oxidation of egg yolk high-density lipoprotein (EYHDL) on the protein structure and emulsification, 2,2'-azobis (2-methylpropionamidine) dihydrochloride (AAPH) was selected as a representative lipid peroxidation-derived peroxyl radical. The results of Raman spectroscopy indicated that, with the increase in the concentration of AAPH, the EYHDL carbonyl content increased significantly and the free sulfhydryl content declined sharply. Circular dichroism spectroscopy and intrinsic fluorescence indicated that exposure of EYHDL to AAPH led to destruction of the orderly structure and reduction of the structural stability. The particle size distribution and zeta potential indicated that the peroxyl radical caused molecular aggregation. Moderate oxidizing conditions can enhance the emulsification of EYHDL, and high-intensity oxidation decreased emulsification. The research results indicated that EYHDL made a significant change in the oxidation system and led to a change in its structure and emulsification, providing a theoretical basis to clarify the EYHDL oxidation mechanism. PRACTICAL APPLICATIONS: Egg yolk powder is prone to emulsification degradation during storage. The emulsification of egg yolk powder is mainly derived from high-density lipoprotein in egg yolk. Moreover, egg yolk powder contains a large amount of lipids, and, during the processing and storage of egg yolk powder, many lipid peroxyl radicals are inevitably generated. Therefore, it is desired to combine the lipid peroxyl radicals generated during the storage of egg yolk powder with the decrease in emulsifiability. In this paper, we first investigated the effects of peroxyl radicals on the structure and emulsifying properties of high-density lipoproteins and provided a theoretical basis to solve the problem that the emulsifiability of egg yolk powder is significantly reduced during storage.

LİYOFİLİZE BILDIRCIN YUMURTASI, YUMURTA BEYAZI VE YUMURTA SARISININ KÖPÜK OLUŞTURMA VE EMÜLSİFİKASYON ÖZELLİKLERİNİN BELİRLENMESİ

The aim of the study was to investigate the foaming and emulsifying properties of lyophilized whole quail egg, egg yolk and egg white. The microstructural properties of oil-in-water emulsions of egg powders were determined and rheological properties of mayonnaise-like product produced using egg yolk powder were investigated. The highest foaming capacity was recorded for egg white powder at all pH levels. Egg yolk powder at 1% dispersion level had the highest emulsion activity index value (P<0.05). Micrographs of scanning electron microscope (SEM) proved that the finest emulsion was prepared using egg yolk powder. Mayonnaise-like product produced using egg yolk powder exhibited gel-like structure (G’>G”) with shear thinning behavior. Quail egg yolk powder was successfully used in production of a product having acceptable rheological properties. Consequently, it was shown that lyophilized quail egg powders had potential to be used as alternative foaming and/or emulsifying agents in food products.

Protective effect of chicken egg yolk immunoglobulins (IgY) against enterotoxigenic Escherichia coli K88 adhesion in weaned piglets

BackgroundEnterotoxigenic Escherichia coli K88 (E. coli K88) are considered as a major cause of diarrhea and death in newly weaned piglets. Oral passive immunization with chicken egg yolk immunoglobulins (IgY) have attracted considerable attention for treatment of gastrointestinal infection due to its high specificity. In this study it was estimated the protective effect of anti-K88 fimbriae IgY against E. coli K88 adhesion to piglet intestinal mucus in vitro and to investigate the potential use of IgY for controlling E. coli-induced diarrhea in weaned piglets in vivo.ResultsE. coli K88 was incubated with IgY for 24 h, and the bacterial growth profiles showed that specific IgY with a concentration higher than 5 mg/mL was observed to significantly inhibit the growth of E. coli K88 compared to nonspecific yolk powder in a liquid medium. Moreover, pretreatment with 50 mg/mL of IgY was found to significantly decrease the adhesion ability of E. coli K88 to porcine jejunal and ileal mucus, further supported by the observations from our immunofluorescence microscopic analysis. In vivo, administration of IgY successfully protected piglets from diarrhea caused by E. coli K88 challenge. Additionally, IgY treatment efficiently alleviated E. coli-induced intestinal inflammation in piglets as the gene expression levels of inflammatory cytokines TNF-α, IL-22, IL-6 and IL-1β in IgY-treated piglets remained unchanged after E. coli K88 infection. Furthermore, IgY significantly prevented E. coli K88 adhering to the jejunal and ileal mucosa of piglets with E. coli infection and significantly decreased E. coli and enterotoxin expression in colonic contents.ConclusionOutcome of the study demonstrated that IgY against the fimbrial antigen K88 was able to significantly inhibit the growth of E. coli K88, block the binding of E. coli to small intestinal mucus, and protect piglets from E. coli-induced diarrhea. These results indicate that passive immunization with IgY may be useful to prevent bacterial colonization and to control enteric diseases due to E. coli infection. The study has great clinical implication to provide alternative therapy to antibiotics in E coli induced diarrhea.

Effect of enzymatic hydrolysis on the solubility of egg yolk powder from the changes in structure and functional properties

Egg yolk powder (EYP) is widely used in food processing due to its excellent emulsifying properties, but its poor solubility limits its market application as a powdered beverage. In this study, papain, trypsin, and subtilisin were used to hydrolyze egg yolk, and the effects of enzymatic hydrolysis on the solubility of EYP was investigated. The results showed that subtilisin treatment significantly improved the water dispersibility, powder solubility and stability coefficient of EYP (P < 0.05) by 41.67%, 98.16%, and 66.21%, respectively, while its solubility did not change significantly after the treatment with papain and trypsin. The degree of hydrolysis (DH) was related to the enzyme active site and the treatment with papain and subtilisin resulted in a higher DH. Additionally, the results of sodium dodecyl sulfate-polyacrylamide gel electrophoresis, particle size distribution and scanning electron microscopy proved that the molecular weight of subtilisin-treated EYP was lower than EYP after the other treatments. Moreover, surface hydrophobicity and zeta-potential results indicated that molecular hydrophobicity and the amount of surface charge increased after enzymatic hydrolysis. The hydratability of papain-treated EYP increased to 20.91%. Low-field nuclear magnetic resonance results showed that enzymatic hydrolysis changed the content of bound water in the EYP, resulting in the changes of its ability to combine with water.

In vitro efficacy of potentiated egg yolk powder against Campylobacter jejuni does not correlate with in vitro efficacy.

Campylobacter jejuni is a zoonotic agent responsible for the foodborne gastroenteritis campylobacteriosis. Control of C. jejuni load in the poultry primary production is recognized as an avenue to reduce human exposure to the pathogen. As for now, no commercially applicable control methods exist at the farm. Several studies tested egg yolk powders, potentiated or not against C. jejuni, as feed additives for chicken and suggested that the quantity and quality of the antibodies presence in the yolk are determinant factors for the full success of this approach. Unfortunately, data from these studies inconsistently showed a reduction of cecal C. jejuni carriage. Our first goal wwas to characterize (quantification by ELISA, agglutination test, bacterial antigen recognition profiles by Western blot, bactericidal effect by serum killing assays and C. jejuni mobility by soft agar migation) the antibodies extracted from egg yolk powders originating from different egg production protocols. Secondly, these powders were microencapsulated and recharacterized. Finally the protected powders were tested as a feed additive to destabilize C. jejuni colonization in an in vivo assay. Despite the in vitro results indicating the ability of the egg yolk powders to recognize Campylobacter and potentially alter its colonization of the chicken caecum, these results were not confirmed in the in vivo trial despite that specific caecal IgY directed toward Campylobacter were detected in the groups receiving the protected powders. More research is needed on Campylobacter in order to effectively control this pathogen at the farm.

Physicochemical Properties of Egg Yolk Powder from Eggs of Different Types of Bird

The goal of this study was to determine whether the physicochemical properties of egg yolk powders produced from eggs from different types of bird (Fighting chicken, Kampung chicken, Serama chicken, Leghorn chicken, Guineafowl, and Turkey) differ from each other and from those of commercial egg yolk powder. The powders were analysed to determine yield; proximate composition; colour, solubility; water holding capacity (WHC); and emulsion activity and stability. Egg yolk powders were prepared by separating the egg yolk manually followed by blast freezing and freeze drying. The weight of the egg ranged from 22.16 g for Serama to 66.25 g for Turkey. The lowest yield of egg yolk liquid was found in Leghorn egg (27.63) and highest in Serama egg (44.31). Egg yolk powder yield was also lowest for Leghorn eggs (12.85%) , followed by Turkey (15.85%), Guineafowl (16.22%), Kampung (16.48%), Fighting (16.62%), and the highest for Serama (18.92%). All parameters studied except WHC differed significantly (p &lt; 0.05) among at least some of the different egg yolk powders. Egg yolk powder from Serama chicken had the highest protein content (40.77%), lowest fat content (51.96%), highest solubility (20.20 oBrix), and lowest WHC (79.78 %). Egg yolk powder from Fighting chicken had the highest emulsion activity (54.13%) and that from Leghorn chicken had the highest emulsion stability (48.41%). Egg yolk powder from Guineafowl had the highest yellowness intensity (72.21), whereas the value was lowest (35.84) for commercial egg yolk powder. In conclusion, physicochemical properties of egg yolk powder depend on the source of the eggs.

Antidiabetic effects of Olea europaea L. leaves in diabetic rats induced by high-fat diet and low-dose streptozotocin

Ethnopharmacological relevanceDiabetes mellitus (DM) is a metabolic disorder characterized by hyperglycemia, insulin resistance, and dyslipidemia. It has broad occurrence worldwide, affecting millions of people, and can cause serious complications. The olive (Olea europaea L.) has important pharmacological functions, including anti-inflammatory, antioxidant, and hypoglycemic activities. Olive leaves are used in traditional medicine for diabetes and hypertension. Aim of the studyTo evaluate the effect of the ethanolic extract of olive leaves (EEOL) on the metabolism of rats with diabetes induced by a high-fat diet and low dose of streptozotocin (STZ). Materials and methodsMale Wistar rats were either given normal feed or a high-fat diet (70% standard laboratory feed, 15% sucrose, 10% lard and 5% yolk powder) for four weeks, followed by administration of STZ (35 mg/kg, via ip). Animals with fasting glucose levels above 200 mg/dL were considered diabetic. Animals were divided into 5 groups, which received ethanol (10 mL/kg), metformin (250 mg/kg), or EEOL at doses of 200 and 400 mg/kg during 10 weeks by oral gavage. Blood samples were used to measure hematological and biochemical parameters, and kidneys were removed for posterior analysis. Body weight was recorded weekly. ResultsA significant decrease in body weight was observed among diabetic animals treated with ethanol and EEOL compared to the control group. Moreover, animals treated with EEOL showed an improvement in glucose levels and in levels of inflammatory and metabolic markers when compared to diabetic animals. ConclusionsThe results indicate a potential anti-diabetic activity of olive leaves, however more studies are needed to validate clinical effects.

Improved strategy for large scale isolation of sialylglycopeptide (SGP) from egg yolk powder

Chicken egg yolk is an easily available source for the isolation of sialylglycopeptides (SGP) carrying homogenous biantennary N-glycans. This approach has gained much attention in the last decade since these SGPs can easily be used for the semi-synthesis of glycoconjugates circumventing laborious full-synthetic methodologies. Here we report an optimised, significantly shorter (one day instead of five) and environmentally friendly procedure for the mg scale isolation of SGP using commercially available egg yolk powder. A single chromatographic step following chloroform/methanol precipitation of proteins and lipids yielded desired approximately 200 mg SGP from 250 g egg yolk powder within a day.•Environmentally friendly procedure for isolation of sialylglycopeptide from Egg yolk powder.•Reduced the protocol from five days down to one.

Effect of dietary supplementation with hyperimmunized hen egg yolk powder on diarrhoea incidence and intestinal health of weaned pigs

ABSTRACTThis study investigated the effects of dietary supplementation with hyperimmunized egg yolk powder (HEYP) on diarrhoea incidence and intestinal health of weaned pigs. Two kinds of HEYP were used, one is multivalent HEYP against mixed Escherichia coli (E. coli) (K88-K99-987P) antigens and the other is the mixture of three monovalent HEYP against single E. coli K88, K99 or 987P antigen, respectively. A total of 180 weaned pigs were assigned to 5 dietary treatments: control diet (CON), 3 or 30 g/kg of two kinds of HEYP diets. Pigs fed HEYP diets had a lower diarrhoea incidence, lower E. coli numbers in faeces and ameliorated mucosal morphology in the duodenum, accompanied by a tendency to reduce inflammation response and intestinal permeability compared with CON. There were no significant differences in the dosage effects of either HEYP. Therefore, anti-(K88-K99-987P) HEYP might be an effective feed additive to protect weaned pigs against enterotoxigenic E. coli⁠.

Antioxidant potential of theaflavin ameliorates the activities of key enzymes of glucose metabolism in high fat diet and streptozotocin – induced diabetic rats

ABSTRACTObjectives: The present study was to evaluate the effect of theaflavin on the activities of key enzymes of carbohydrate metabolism in high fat diet and streptozotocin – induced diabetic rats.Methods: Diabetes was induced in male albino Wistar rats by feeding them with high fat diet comprising of standard laboratory rat chow 84.3%, lard 5%, egg yolk powder 10%, cholesterol 0.2% and bile salt 0.5% for 2 weeks. After 2 weeks, the animals were kept in an overnight fast and injected with low dose of streptozotocin (40 mg/kg b.w).Results: Theaflavin (100 mg/kg b.w /day) was administered orally to diabetic rats for 30 days. At the end of the experimental period, diabetic control rats showed significant increase in plasma glucose, homeostatic model assessment of insulin resistance (HOMA-IR), glycosylated hemoglobin (HbA1c) with concomitant decrease in plasma insulin, total hemoglobin and body weight. The activities of key enzymes of carbohydrate metabolism, lipid peroxidation markers, antioxidant enzymes, glycogen content and glycogen synthase and glycogen phosphorylase were also altered in diabetic rats.Discussion: Oral administration of theaflavin to diabetic rats significantly ameliorated all the biochemical alterations to near normal levels. The results of the present study suggest that theaflavin exhibits antidiabetic effect through its antioxidant activity.

Enterobacter cloacae associated with mass mortality in zoea of giant freshwater prawns Macrobrachium rosenbergii and control with specific chicken egg yolk immunoglobulins (IgY)

Outbreaks of mass mortality among cultured zoea of M. rosenbergii occurred in different hatcheries of Gaoyou country of China in April 2017. Of the isolates obtained from the diseased zoea, XL3–1 was pathogenic to the zoea and postlarva of M. rosenbergii in the virulence test, with a LD50 value 3.2 × 106 CFU/mL and 3.5 × 106 CFU/mL, respectively. XL3–1 was identified as Enterobacter cloacae by biochemical characteristics and molecular tools. Detection of virulence-associated genes by PCR indicated that XL3–1 was positive for irp2, fhuA, sodB, sltA, flaD and OmpX. According to the antibiotic resistant assay, the isolate was sensitive to quinolones, while they were resistant to cephalosporins and penicillins. This study also investigated the protection of specific chicken egg yolk antibodies (IgY) in E. cloacae-infected M. rosenbergii. The highest titer against E. cloacae of the obtained specific IgY by ELISA was 1: 213. M. rosenbergii treated with specific egg yolk powder could achieve the survival rate of 67% after E. cloacae infection, while M. rosenbergii treated with non-specific egg yolk powder or no egg yolk powder could achieve survival rates of 20% and 17%, respectively. As well, the bacterial burden in hepatopancreas was significantly lower in M. rosenbergii treated with specific egg yolk powder than those treated with non-specific egg yolk powder. This study clarified the virulence characteristics and antibiotic resistance of E. cloacae, and provided a strategy to control the disease by using specific IgY.

Bioaccessibility of Biofortified Sweet Potato Carotenoids in Baby Food: Impact of Manufacturing Process.

Orange-fleshed sweet potato (OFSP), a biofortified crop rich in β-carotene, can be used as a component of baby food recipes in order to tackle vitamin A deficiency in children <6 years old. In this work, the impact of formulation (addition of pumpkin, oil, and egg yolk) and industrial heat processing (pasteurization, sterilization) on carotenoid content and bioaccessibility was evaluated in an OFSP-based baby puree. A commercial OFSP baby food product from Brazil and a homemade OFSP puree were used as references. The losses of all-trans-β-carotene ranged from 16 to 21% (pasteurization, homemade) to 32% (sterilization). Because of higher particle sizes and despite a higher content in carotenoids, the homemade puree had a lower bioaccessibility (i.e., micellar transfer using in vitro digestion: 0.50%) compared with the sterilized and commercial purees (5.3–6.2%). Taking into account bioaccessibility and applying a 50% conversion to retinol, a 115 g baby portion of the sterilized OFSP-puree formulated with 2% oil provided 31.4% of the daily vitamin A requirement (RDA) for children under 6 years. In comparison, 115 g of homemade OFSP-puree provided only 3.5% of the RDA. Addition of pumpkin to OFSP did not improve the percentage of RDA. Interestingly, the incorporation of an emulsifier (egg yolk powder) before cooking could improve the percentage of provision by a factor of 2.7. These results showed that reaching a balance between formulation and processing is determinant to maximize carotenoid bioaccessibility of carotenoids from OFSP-based baby food.

PO-020 Aerobic Exercise Attenuates Myocardial Injury Through Activation of SIRT1 Signaling in T2DM Rats

Objective Myocardial injury caused by elevated blood glucose is a major risk factor for type 2 diabetes mellitus (T2DM) cardiomyopathy. Aerobic exercise can significantly improve the energy metabolism and is widely used in clinic to prevent and cure T2DM and other metabolic diseases. Myocardial injury can be attenuated after aerobic exercise. Some researches showed SIRT1 is a histone deacetylation enzyme activated by NAD+/NADH, and mainly distributes in the heart, liver, etc. SIRT1 plays an important role in controlling the insulin secretion, which can regulate glucose and lipid metabolism and some other important biological functions. It is not known whether the myocardial injury was reduced by regulating the level of SIRT1 after aerobic exercise. The purpose of the research was to illustrate the regulatory mechanism of decreased myocardial injury after aerobic exercise, and provided theoretical basis for early prevention and treatment of T2DM myocardial injury. &#x0D; Methods There were two stages in the experiment. At the first stage, 30 male SD rats,12-month old, were randomly divided into two groups, the control group (CC, fed with standard diet and kept sedentary, 8 rats), the high-sugar-lipid fodder for T2DM model Group (DC, kept sedentary, 22 rats). High sugar and high fat diet formula for 10% lard, 20% sucrose, egg yolk powder 8%, 0.1% sodium deoxycholate, 61.9% basic feed, AIN-93g, provided by animal experimental center of Hebei Province. After 5-week high-sugar-lipid fodder, the DC rats were injected streptozocin (STZ, 35mg/kg), the FBG of the tail vein were measured after 12h, and FBG≥7.0mmol/L was defined as T2DM model. Six rats were excluded because of low FBG (&lt;7.0mmol/L). At the second stage, all of the rats were fed with standard diet. The T2DM rats were randomly assigned to the T2DM group (DC, kept sedentary, 8 rats) and T2DM combined with aerobic exercise, (DE, run on treadmill at 15m/min～19m/min, 45min/d, slope 5%, 6d/w, 5W, 8 rats). The myocardial tissue sections were stained with hematoxylin and eosin (HE) to observe the histological changes. The level of serum insulin was examined by ELISA kits. The content of serum glucose, the activity of SOD and the content of MDA in heart were examined by reagent kits after 5 weeks. The expression of SIRT1 protein in heart were measured using Western blot. ELISA kits were used for the determination of the NAD+/NADH ratio. &#x0D; Results (1) The myocardial sections of CC can be observed clear cell contour, bright color, arranged closely and neatly, and nuclear distribution in cells border and nuclei of uniform size. In DC myocardial sections, myocardial cell contour was fuzzy, nuclei were relatively large, reduced the number of nuclei and inward migration. The broken cells and part of the cells and nuclei overflow can also be seen. The shape of nucleus was irregular and shifted to the internal. In myocardial cell sections of DE rats, the cell profile was relatively clear, and arranged more orderly, a larger number of nuclei, generated less shift. (2) The plasma insulin of DE were obviously lower than that of DC (P&lt;0.01). The content of serum glucose of DE was significantly decreased compared with that of DC (P&lt;0.01). (3) The ratio of NAD+/NADH in DE heart was higher than that of DC (P&lt;0.05). (4) The expression of SIRT1 in DE heart was higher than that of DC (P&lt;0.05). (5)The activity of SOD in DE heart was increased while the content of MDA in DE was significantly decreased compared with those in DC (P&lt;0.01). &#x0D; Conclusions Aerobic exercise can effectively reduced the blood glucose level of T2DM rats. The NAD+/NADH ratio in the myocardium of T2DM rats were increased after the aerobic exercise. As a result, the content of SIRT1 protein in myocardial cells of T2DM rats were increased after the aerobic exercise, which resulted in the increased SOD activity and antioxidant capacity in the cardiac muscle cells, which lead to the attenuated myocardial injury in T2DM rats .

Immunomodulatory effects of chicken egg yolk antibodies (IgY) against experimental Shewanella marisflavi AP629 infections in sea cucumbers (Apostichopus japonicus)

Skin ulceration syndrome in sea cucumbers is an infectious bacterial disease with fast and high mortality. This study investigated the protection of chicken egg yolk antibodies (IgY) on skin ulcer syndrome in sea cucumbers induced by intraperitoneally injecting Shewanella marisflavi AP629. Inactivated whole S. marisflavi AP629 cells were used as an immunogen to immunize laying hens. The highest titer of the obtained specific IgY by ELISA was 1:90000. Specific IgY significantly inhibited the growth of S. marisflavi AP629 in a liquid medium, dose-dependent manner at concentrations ranging from 0.5 to 2 mg/mL. Results obtained from scanning electron microscopy and confocal laser scanning microscopy showed that specific IgY could make bacteria agglutinate and damage the cell membrane of S. marisflavi AP629, resulting in a decrease of bacterial viability. Sea cucumbers treated with 25, 5, and 1 mg/mL anti-S. marisflavi AP629 IgY could achieve survival rates of 77.5%, 50%, and 22.5% at day 12 when the infection and injection therapy were carried out at the same time, respectively. However, survival rates of sea cucumbers treated with 25 mg/mL of nonspecific IgY were only 7.5% at day 12. All sea cucumbers in the positive control group died within twelve days after bacterial inoculation. Levels of the five humoral immune factors (LYZ, ACP, NOS, SOD, CAT) released by coelomocytes were significantly increased in the specific IgY group compared to the nonspecific IgY and positive control groups within 12 h. However, the activities of LYZ, ACP, and SOD decreased rapidly at the 48 h time point in the specific IgY group, indicating that specific IgY treatment could shorten the time needed to restore balance in sea cucumber immune systems. Oral prophylaxis with egg yolk powders was that all sea cucumbers were challenged with 4.2 × 106 CFU S. marisflavi AP629 by intraperitoneal injection after 60 days of feeding. Survival rates of diets containing 10%, 5%, and 1% specific egg yolk powder were 57.5%, 52.5%, and 30% by day 12, respectively, and the survival rate was 27.5% for the nonspecific group and 22.5% for the positive control group. After feeding for 60 days, enzyme activities of LZY, NOS, and SOD were all significantly enhanced in sea cucumbers fed with specific egg yolk powder when compared to the control group (p < 0.05). This study demonstrated that the phagocytic activities of coelomocytes were significantly stimulated after specific IgY treatment over that of nonspecific IgY or without IgY treatments in sea cucumbers (p < 0.05). Overall, our results revealed that anti-S. marisflavi AP629 IgY has a positive immunomodulatory effect on sea cucumbers infected with S. marisflavi AP629.

Acyclic Isoprenoid Attenuates Lipid Anomalies and Inflammatory Changes in Hypercholesterolemic Rats.

The present study was aimed to explore the antihyperlipidemic and anti-inflammatory effect of acyclic isoprenoid on high fat diet fed rats. Hypercholesterolemia was induced by the diet comprising of the normal rat chow 84.3%, lard 5%, yolk powder 10%, cholesterol 0.2% and 0.5% bile salt were fed to the rats for the period of 8weeks. The results showed that abnormally elevated levels of plasma lipid profiles. Three different doses of acyclic isoprenoid (20, 40 and 80mg/kg b.w/day) were administered orally to hypercholesterolemia suffering rats for the period of 30days. Among these three doses of acyclic isoprenoid, the dose 80mg/kg b.w. was significantly decreased the plasma lipid profiles when compared to other two doses. The effect produced by acyclic isoprenoid (80mg/kg b.w) was comparable to that of simvastatin. Therefore, 80mg/kg b.w was fixed as a effective dose and used for further analyses. Acyclic isoprenoid administration reinstated the elevated levels of cardiac and inflammatory markers in both blood and serum of hypercholesterolemic rats. In addition, acyclic isoprenoid administration decreased activity of 3-hydroxy 3-methyl-glutaryl-CoA reductase and increased the activity of lecithin cholesterol acyl transferase. These findings suggest that the administration of acyclic isoprenoid was potentially ameliorated the cardiac marker enzymes and inflammatory markers in addition to its antihypercholesterolemic effect.

Health benefits of orally administered anti-IL-10 antibody in milk-fed dairy calves

The primary objective of this randomized controlled trial was to determine whether anti-IL-10 egg yolk antibodies fed upon arrival to a calf ranch would lower the prevalence of Cryptosporidium parvum shedding in naturally challenged preweaned dairy calves. The secondary objectives included measuring the effect of anti-IL-10 antibodies on calf health, performance, and shedding of less common diarrheal pathogens. A total of 133 calves, enrolled at 24 to 72 h of age, received a daily dose of 0.96 g of egg yolk powder with anti-IL-10 antibodies (MAB, n = 71) or without anti-IL-10 antibodies (MEP, n = 62) split between 2 feedings for the first 11 d on feed at a calf ranch. Daily health evaluations were completed for 15 d after arrival and on d 56. Digital weights were collected at enrollment and d 56, and hipometer weights were collected at enrollment and d 7 and 56. Packed cell volume and serum total protein concentration were measured at enrollment and on d 7 and 14. Fecal pH was measured at enrollment and on d 5 and 14, and fecal pathogen (C. parvum, coronavirus, rotavirus, and Salmonella spp.) shedding was assessed at d 5 and 14. Continuous outcomes were compared between groups using a Student's t-test or Wilcoxon rank sum test. Fecal pathogen shedding at d 14, respiratory disease at d 56, and antibiotic usage were compared using relative risk (RR) and chi-squared test. Fecal pH (median and interquartile range) on d 14 was 6.65 (6.39–6.99) and 6.52 (5.97–6.81) for MAB and MEP, respectively. On d 56, the risk of respiratory disease was lower for MAB compared with MEP (RR = 0.40; confidence interval = 0.16–0.99). The risk for antibiotic treatment was lower for MAB- compared with MEP-treated calves (RR = 0.38; confidence interval = 0.17–0.88). The risk of shedding rotavirus was higher in MAB (RR = 1.38; confidence interval = 1.10–1.81) calves. After multivariable analyses, hipometer weights (least squares means ± standard error) were 1.7 ± 0.8 kg greater on d 56 in MAB compared with MEP; however, ADG was 0.04 ± 0.02 kg/d lower in MAB calves. Total health score, diarrhea days, average respiratory score, packed cell volume, and serum total protein were not affected by feeding anti-IL-10 egg antibodies. In summary, feeding anti-IL-10 antibodies was associated with increased fecal pH, reduced risk of respiratory disease later in the preweaning period, and decreased antibiotic usage despite higher rotavirus infection. These findings might be associated with improved mucosal immunity, enhanced host defenses, or reduced susceptibility and warrant further investigation.

Isoespintanol improves the post-thaw quality of stallion semen

The present study was conducted to assess the effect of the egg yolk powder in place of fresh egg yolk (EY) in the semen diluent on semen morphological attributes (SMAs) and mobility &amp; velocity profiles measured by computer assisted sperm analyser (CASA) in cryopreserved mithun semen. A total of 25 ejaculates were collected through rectal massage method from five healthy mithun bulls (five ejaculates in each bull) and diluted with the standard tris citrate glycerol (TCG) extender and were splited into four equal aliquots: Gr I: control (20% EY); Gr II, III and IV contained 5, 8 and 10% EY powder, respectively. SMAs, CASA parameters were evaluated following freezingthawing of semen. Result revealed significant (p&lt;0.05) improvement was observed in these SMAs and CASA parameters in Gr II and III as compared to Gr I and IV. It was concluded that inclusion of 5% or 8% EY powder in semen diluent holds a clear advantage and higher benefits over 20% fresh EY in cryopreservation of mithun semen.

Effect of egg yolk powder on cryopreservation of mithun semen

The present study was conducted to assess the effect of the egg yolk powder in place of fresh egg yolk (EY) in the semen diluent on semen morphological attributes (SMAs) and mobility &amp; velocity profiles measured by computer assisted sperm analyser (CASA) in cryopreserved mithun semen. A total of 25 ejaculates were collected through rectal massage method from five healthy mithun bulls (five ejaculates in each bull) and diluted with the standard tris citrate glycerol (TCG) extender and were splited into four equal aliquots: Gr I: control (20% EY); Gr II, III and IV contained 5, 8 and 10% EY powder, respectively. SMAs, CASA parameters were evaluated following freezingthawing of semen. Result revealed significant (p&lt;0.05) improvement was observed in these SMAs and CASA parameters in Gr II and III as compared to Gr I and IV. It was concluded that inclusion of 5% or 8% EY powder in semen diluent holds a clear advantage and higher benefits over 20% fresh EY in cryopreservation of mithun semen.

Comparative study on physicochemical and functional properties of egg powders from Japanese quail and white Leghorn chicken

ABSTRACTPhysicochemical and functional properties of freeze-dried egg powders (egg white, egg yolk, and whole egg) from Japanese quail and white Leghorn chicken were studied comparatively. All egg powders had protein content in the range of 91.13–97.03 g/100 g powder. The quail egg powder had higher mineral and essential amino acid contents, but lower fat content as compared to chicken egg powders (P < 0.05). Moreover, egg white powder from both quail and chicken presented higher total amino acids content than corresponding whole egg and egg yolk powders, respectively. Fourier transform infrared spectroscopic study revealed that β-sheet is the major secondary structure of all egg powders. Based on differential scanning calorimetry analysis, quail egg powders showed slightly lower denaturation temperatures than corresponding chicken egg powders (P < 0.05). Sodium dodecyl sulfate–polyacrylamide gel electrophoresis study showed the slight difference in protein patterns of corresponding quail and chicken egg powders. The quail egg powders presented higher protein solubility than corresponding chicken egg powders at all pH tested. Furthermore, quail egg powders exhibited higher emulsion activity index and emulsion stability index with higher foam expansion and stability than the corresponding chicken egg powders. Therefore, Japanese quail egg powders could be used as an alternative to white Leghorn chicken egg in the preparation of foods and diets that require high protein content with positive health benefits.