This study aims to determine the level of physical fitness index and mental health of lecturers and educational staff at Yogyakarta State University. The research design used in this study uses an experimental research method. The population of this study were lecturers and educational staff at Yogyakarta State University. The instrument for measuring physical fitness used the Rockport Test. The instrument for determining the level of mental health used the Self Reporting Questionnaire-20 (SRQ-29). The study was conducted at the UNY Athletics and Football Stadium and the UNY Wates Stadium. The sample consisted of 202 UNY Lecturers and 62 UNY Educational Staff. The results of the study that have been carried out, obtained the results that the level of physical fitness of UNY Lecturers was 1% indicating good, 3% indicating very poor, 43% indicating sufficient, 53% indicating less. While for the percentage results of the Fitness Test for UNY Educational Staff, 13% indicated good, 2% indicated very poor, 48% indicated sufficient, 37% indicated less. Meanwhile, the mental health test of UNY Education Staff showed that 92% were good, 6% were quite good and 2% were lacking. Meanwhile, the mental health test of UNY Lecturers showed that 97% were good, 2% were quite good and 1% were lacking.