Oyster mushroom occupy important place in quality rich food due to their delectability & nutritive enrichment for living being also used to grow widely more in agro waste & household waste showing Bioremediation effect on waste material by breakdown into a suitable media to grow Mushroom mycelium. Unique data obtained showing assembles its food by secreting degrading enzymes, its decomposing the complex organic materials on which it grows the substrate to generate simpler Compounds for Its nutrition. The product we carried or substrate use are house hold waste like raw residue Wheat flour, rice flour, gram flour and agricultural ashes of wheat (choker),rice coverings, burned and filtered teas, raw vegetables washing, utensils washing water. Are considering as wastes these waste, are not carefully disposed of in surrounding by dumping or burning type of vernacular method create lot of pollution. Oyster create a ecologic balance Link to use this waste as a substrate to grow the mushroom mycelium fast increasing Mushroom yield too by the process of bioremediation. mushroom cultivation reports that the economical viable biotechnology process for conversion of waste food residues , ashes agriculture waste & washing water at the outlet fits very well into this category, after treating dry sterilization or wet sterilization of all substrate For growth promotion test Of given substrate By pour plate Method on the select given media compositing with these three types of waste in triplicates (G1)Raw food stuff in equal ratio like Wheat flour, rice flour, gram flour(G2)Ashes of wheat (choker),rice coverings, burned and filtered teas,(G3)Vegetable Washings, utensil washing ,kitchen cleaning water, In growth promotion test showing the maximum colonies in raw food stuff then ashes than washing water shown by minimum concentration of waste or unused product. Pleurotus Ostreatus act as a scavenging agent for the waste food Stuff creating new Fruiting mushroom body to grow In it & Utilized to environment cleanups. Keywords : Pleurotus Ostreatus, Growth promotion test, Pour plate method, Bioremediation.