Yield Penalty Research Articles

Identifying Quality Protein Maize Inbred Lines for Improved Nutritional Value of Maize in Southern Africa.

Malnutrition, as a result of deficiency in essential nutrients in cereal food products and consumption of a poorly balanced diet, is a major challenge facing millions of people in developing countries. However, developing maize inbred lines that are high yielding with enhanced nutritional traits for hybrid development remains a challenge. This study evaluated 40 inbred lines: 26 quality protein maize (QPM) lines, nine non-QPM lines, and five checks (three QPM lines and two non-QPM lines) in four optimum environments in Zimbabwe and South Africa. The objective of the study was to identify good-quality QPM inbred lines for future hybrid breeding efforts in order to increase the nutritional value of maize. The QPM lines had a lower protein content (7% lower) than that of the non-QPM lines but had 1.9 times more tryptophan and double the quality index. The lysine- and tryptophan-poor α-zein protein fraction was 41% lower in QPM than in non-QPM, with a subsequent increase in γ-zein. There was significant variation within the QPM inbred lines for all measured quality characteristics, indicating that the best lines can be selected from this material without a yield penalty. QPM lines that had both high protein and tryptophan levels, which can be used as parents for highly nutritious hybrids, were identified.

Earlier crop flowering caused by global warming alleviated by irrigation

Enabling crop flowering within an optimal calendar window minimises long-term risk of abiotic stress exposure, improving prospects for attaining potential yield. Here, we define the optimal flowering period (OFP) as the calendar time in which long-term risk of frost, water and heat stress are collectively minimised. Using the internationally-renowned farming systems model Agricultural Systems Production Systems sIMulator, we characterised combined effects of climate change and extreme climatic events on the OFPs of barley, durum wheat, canola, chickpeas, fababean and maize from 1910 to 2021. We generate response surfaces for irrigated and dryland conditions using a range of representative sowing times for early and late maturity genotypes. Global warming truncated crop lifecycles, shifting forward flowering of winter crops by 2–43 d in dryland environments, and by −6–19 d in environments with irrigation. Alleviation of water stress by irrigation delayed OFPs by 3–25 d or 11–30 d for early and late maturity winter crops, respectively, raising average yields of irrigated crops by 44%. Even so, irrigation was unable to completely negate the long-term yield penalty caused by the climate crisis; peak yields respectively declined by 24% and 13% for rainfed and irrigated crops over the 111 years simulation duration. We conclude with two important insights: (a) use of irrigation broadens OFPs, providing greater sowing time flexibility and likelihood of realising potential yields compared with dryland conditions and (b), the most preferable maturity durations for irrigated winter and summer crops to maximise potential yields are early-sown long-season (late) and later-sown short-season (early) maturity types, respectively.

Genome sequencing and genetic analysis of recombinant inbred lines reveals important agronomic traits related loci under different nitrogen fertilization.

The intensive application of inorganic nitrogen has contributed to increasing the crop yield with the risk of environmental damage. High nitrogen fertilizer requirements are a long-standing problem in japonica cultivation. In the present study, 200 recombinant inbred lines derived from two representative japonica varieties of Japanese and Chinese varieties, Akitakomachi and Liaogeng5, respectively, were planted under different nitrogen fertilization conditions. The relationships among nitrogen fertilization, genetic background, and important agronomic traits were surveyed through the phenotypic investigation and next-generation sequencing. The results showed that the yield components of Akitakomachi dramatically decreased along with the nitrogen reduction, whereas those of Liaogeng5 exhibited a slight decrease. The appearance quality and milling quality of both Akitakomachi and Liaogeng5 were improved, especially Liaogeng5. The recombinant inbred lines (RILs) showed similar trends to their parental lines. The QTLs analysis of important agronomic traits detected 17 loci, one QTL clustering located on chromosome 9 that corresponded to the plant height, panicle angle, grain number per panicle, harvest index, and taste value was identified to be synonymous with a previously reported gene, DEP1. The function of DEP1 was verified by a knock-out mutant constructed by CRISPR/Cas9. The dep1 mutant exhibited improved grain quality without severe yield penalty under nitrogen reduction conditions. The results of the present study provide useful information for the improvement of food security by enhancing nitrogen use efficiency.

Institutional analysis for scaling alternate wetting and drying for low-emissions rice production: evidence from Bangladesh

ABSTRACT This article provides a comprehensive institutional analysis to better understand the key conditions and incentives relevant for the uptake of alternate wetting and drying (AWD) – a climate-smart irrigation practice in Bangladesh. Practicing AWD in rice production can reduce water consumption by 30% and mitigate methane emissions by 30–70% without yield penalty. Primary research was conducted in Rangpur, Rajshahi, Mymensingh, and Sherpur Divisions. The analysis depicts the dynamic among the stakeholders involved in scaling AWD using the Net-Map tool, and demonstrates the influence of irrigation management systems in the selected catchment areas and the impacts of AWD in rice farming. Findings reveal that AWD-practicing farmers, pump owners, and the Upazila Irrigation Committee are the three most powerful influencers for scaling AWD. The pre-paid irrigation system significantly improves water efficiency and cost-effectiveness when practising AWD. Additionally, AWD leads to a lower frequency of pumping resulting in reduced costs compared to the continuous-flooding practice. Finally, the results guide the formulation of recommendations to create favourable conditions for AWD scaling, which contributes to reducing greenhouse gas emissions and advancing sustainable rice production in Bangladesh.

Stacking of Pup1 QTL for low soil phosphorus tolerance and bacterial blight resistance genes in the background of APMS6B, the maintainer line of rice hybrid DRRH-3

Phosphorus (P) is one of the macronutrients essential for plant growth and development. Rice (Oryza sativa L.) is sensitive to P starvation and its deficiency influences many key plant functions, resulting in crop yield penalties. Although the hybrid rice segment is well-known for its yield heterosis, P deficiency and bacterial leaf blight diseases are evident limitations. APMS6B, the female parent of DRRH-3 is susceptible to low P and bacterial blight disease. In the present study, the improvement of APMS6B to P starvation and resistance to bacterial leaf blight (BB) was carried out using the marker-assisted backcross breeding approach. Kasalath (+Pup1 QTL) was used as a donor, and a promising IL (ATR 594-1) at BC1F4 generation was identified with 81.15% of recurrent parent genome recovery. Concurrently, this IL was intercrossed with GU-2 (+Xa21 and Xa38). Intercross F1s (ICF1) hybridity was confirmed through foreground selection having maximum RPGR (88.29%) and was selfed to produce ICF2. The resultant progenies were phenotyped for BB using Xoo inoculum (IX-020), simultaneously genotyped with gene-specific functional SSR markers forXa21 and Xa38. The identified BB-resistant plants were subjected to foreground selection for Pup1. Four promising ICF3 plants (BP-10-1, BP-10-3, BP-10-5, and BP-10-15 with Xa21, Xa38, and Pup1) along with parents and checks were screened both in low P plot (< 2 kg P2O5 ha−1) as well as in normal plot (> 25 kg P2O5 ha−1) during dry and wet seasons 2018. The field evaluation identified four promising intercrossed lines with better root growth in the primary root length of extracted zone and root volume. In addition, fewer reductions in grain yield (39.10%) under P starvation and less susceptibility indices values (< 1) for BB were observed. These lines may be exploited in the CMS conversion and development of climate-resilient, biotic and abiotic stress-tolerant rice hybrids.

Anthocyanin-Rich Vegetables for Human Consumption-Focus on Potato, Sweetpotato and Tomato.

Malnutrition, unhealthy diets, and lifestyle changes have become major risk factors for non-communicable diseases while adversely impacting economic growth and sustainable development. Anthocyanins, a group of flavonoids that are rich in fruits and vegetables, contribute positively to human health. This review focuses on genetic variation harnessed through crossbreeding and biotechnology-led approaches for developing anthocyanins-rich fruit and vegetable crops. Significant progress has been made in identifying genes involved in anthocyanin biosynthesis in various crops. Thus, the use of genetics has led to the development and release of anthocyanin-rich potato and sweet potato cultivars in Europe and the USA. The purple potato ’Kufri Neelkanth’ has been released for cultivation in northern India. In Europe, the anthocyanin-rich tomato cultivar ‘Sun Black’ developed via the introgression of Aft and atv genes has been released. The development of anthocyanin-rich food crops without any significant yield penalty has been due to the use of genetic engineering involving specific transcription factors or gene editing. Anthocyanin-rich food ingredients have the potential of being more nutritious than those devoid of anthocyanins. The inclusion of anthocyanins as a target characteristic in breeding programs can ensure the development of cultivars to meet the nutritional needs for human consumption in the developing world.

Global benefits of non-continuous flooding to reduce greenhouse gases and irrigation water use without rice yield penalty.

Non-continuous flooding is an effective practice for reducing greenhouse gas (GHG) emissions and irrigation water use (IRR) in rice fields. However, advancing global implementation is hampered by the lack of comprehensive understanding of GHGs and IRR reduction benefits without compromising rice yield. Here, we present the largest observational data set for such effects as of yet. By using Random Forest regression models based on 636 field trials at 105 globally georeferenced sites, we identified the key drivers of effects of non-continuous flooding practices and mapped maximum GHGs or IRR reduction benefits under optimal non-continuous flooding strategies. The results show that variation in effects of non-continuous flooding practices are primarily explained by the UnFlooded days Ratio (UFR, that is the ratio of the number of days without standing water in the field to total days of the growing period). Non-continuous flooding practices could be feasible to be adopted in 76% of global rice harvested areas. This would reduce the global warming potential (GWP) of CH4 and N2 O combined from rice production by 47% or the total GWP by 7% and alleviate IRR by 25%, while maintaining yield levels. The identified UFR targets far exceed currently observed levels particularly in South and Southeast Asia, suggesting large opportunities for climate mitigation and water use conservation, associated with the rigorous implementation of non-continuous flooding practices in global rice cultivation.

Genome-edited powdery mildew resistance in wheat without growth penalties.

Disruption of susceptibility (S) genes in crops is an attractive breeding strategy for conferring disease resistance1,2. However, S genes are implicated in many essential biological functions and deletion of these genes typically results in undesired pleiotropic effects1. Loss-of-function mutations in one such S gene, Mildew resistance locus O (MLO), confers durable and broad-spectrum resistance to powdery mildew in various plant species2,3. However, mlo-associated resistance is also accompanied by growth penalties and yield losses3,4, thereby limiting its widespread use in agriculture. Here we describe Tamlo-R32, a mutant with a 304-kilobase pair targeted deletion in the MLO-B1 locus of wheat that retains crop growth and yields while conferring robust powdery mildew resistance. We show that this deletion results in an altered local chromatin landscape, leading to the ectopic activation of Tonoplast monosaccharide transporter 3 (TaTMT3B), and that this activation alleviates growth and yield penalties associated with MLO disruption. Notably, the function of TMT3 is conserved in other plant species such as Arabidopsis thaliana. Moreover, precision genome editing facilitates the rapid introduction of this mlo resistance allele (Tamlo-R32) into elite wheat varieties. This work demonstrates the ability to stack genetic changes to rescue growth defects caused by recessive alleles, which is critical for developing high-yielding crop varieties with robust and durable disease resistance.

Biofertilization with photosynthetic bacteria as a new strategy for mitigating photosynthetic acclimation to elevated CO2 on cherry tomato

The stimulated effects of elevated CO2 concentration decline over time in many C3 plants. However, the phenomenon is a rare occurrence on plants hosting some symbiosis bacteria. The photosynthesis acclimation associated with an extended period of high CO2 concentration was therefore studied in cherry tomato that treated with a kind of photosynthetic bacteria. Bio-fertilization with photosynthetic bacteria, which can fix nitrogen for host crops and contains a variety of growth promoting factors, can play an important role in maintaining photosynthetic activity. In this research, we conducted a CO2 elevation experiment combined different water regimes and inoculation with photosynthetic bacterium, to evaluate the improvement of photosynthesis acclimation by using photosynthesis bacteria, and to explore associated mechanisms for artificial chamber grown cherry tomatoes. As a result of these experiments, we found that elevated CO2 had a positive effect on cherry tomato photosynthetic activity and yield of all treatment. After 55 days of CO2 injection, photosynthetic acclimation was found in the plant of uninoculated photosynthetic bacteria, which were most pronounced in high water treatments. Reduced irrigation amounts decreased the magnitude of photosynthetic acclimation, but relative fruit yield gradually decreased after achieving a maximum. In contrast, the photosynthetic bacteria inoculated plants just showed a slightly down-regulation of photosynthesis under durative high CO2 condition. Because inoculation of photosynthetic bacteria increased the photosynthetic pigment content, mesophyll conductance, maximum carboxylation rate and maximum electron transport rate. Furthermore, inoculation with the photosynthetic bacteria diminished the yield penalties caused by the reduced irrigation treatment through promoting nitrogen content and relieve starch accumulation in tomato leaves compared to those of the uninoculated control. And enhanced yield of inoculated plants grown under durative high CO2 concentration were observed. The results suggest that photosynthesis bacteria application could be a potential means to further improve the CO2 stimulation effect on cherry tomato production.

Impact of Zero Budget Natural Farming on Crop Yields in Andhra Pradesh, SE India

It has been claimed that Zero Budget Natural Farming (ZBNF), a burgeoning practice of farming in India based on low-inputs and influenced by agro-ecological principles, has the potential to improve farm viability and food security. However, there is concern that the success of the social movement fueling the adoption of ZBNF has become out of step with the science underpinning its performance relative to other farming systems. Based on twenty field plot experiments established across six districts in Andhra Pradesh (SE India), managed by locally based farmer researchers, we present the first ‘on the ground’ assessment of ZBNF performance. We show that there is no short-term yield penalty when adopting ZBNF in small scale farming systems compared to conventional and organic alternatives. In terms of treatment response, we observed differences between agro-climatic zones, but in this initial evaluation we cannot recommend specific options tuned to these different contexts.

Need-based nitrogen management of wheat through use of green seeker and leaf color chart for enhancing grain yield and quality

Nitrogen (N) is a major nutrient influencing the wheat grain yield. The experiment was conducted to need-based N application using leaf color chart (LCC) and GreenSeeker on grain yield and quality. The experiment was laid out in split-plot design with the combination of three varieties (WH 1105, HD 2967 and PBW 621) in main plots and seven nitrogen management practices [absolute control, N150 kg/ha, N by LCC ≥ 4 135/125 kg N ha−1, N by LCC ≥ 4 145/135 kg Nha−1, N by LCC ≥ 4 155/145 kg ha−1, Green Seeker (129.3 kg N/ha) and N 225 kg ha−1] in sub-plots replicated thrice. Wheat varieties WH 1105, HD 2967 and PBW 621 showed similar grain yields. The protein content of PBW 621 was better than WH 1105 and HD 2967. GreenSeeker guided 122.9-133.3 kg N ha−1 for wheat varieties found more efficient than blanket N recommendation of 150 kg N ha−1. The N management with LCC 145 kg/ha (LCC ≥ 4:155/45) saved 5 kg N/ha. The N management with LCC 145/135 kg N ha−1 (LCC ≥ 4) saved 15 kg N ha−1 but with a yield penalty of 3.2% as compared to 150 kg N ha−1. Comparable quality with GreenSeeker and LCC 145 kg N ha−1 was observed as compared to blanket 150 kg N ha−1 application.

Going red but not mad: efficient astaxanthin production in tobacco without yield penalty.

Going red but not mad: efficient astaxanthin production in tobacco without yield penalty.

Epigenetic regulation of heat and cold stress responses in crop plants

Environmental insults are inevitable companions of plants during their life-cycle, which significantly impact growth and development and also pose serious yield penalties. In the era of frequently changing climatic conditions, heat and cold stress impose severe threats to the plants. To deal with such situations, plants have evolved genetic and epigenetically regulated mechanisms including DNA methylation and histone modifications along with upregulation of stress-responsive genes. The networks of such genes have also been kept at pace by chromatin regulators under stressed environments. Furthermore, genetic and biochemical understanding of plant responses to heat, and cold stress have revealed the importance and involvement of chromatin regulators, and histone modifications in plant stress memory. The present review focuses on epigenetic alterations such as DNA methylation, histone, and chromatin modifications in response to heat and cold stress in plants.

Tomato receptor-like cytosolic kinase RIPK confers broad-spectrum disease resistance without yield penalties.

Production of reactive oxygen species (ROS) is an important immune response in plant multilayer defense mechanisms; however, direct modification of ROS homeostasis to breed plants with broad-spectrum resistance to disease has not yet been successful. In Arabidopsis, the receptor-like cytosolic kinase AtRIPK regulates broad-spectrum ROS signaling in multiple layers of the plant immune system. Upon treatment with immune elicitors, AtRIPK is activated and phosphorylates nicotinamide adenine dinucleotide phosphate (NADPH) oxidase, which leads to ROS production. In this study, we identified an AtRIPK ortholog in tomatoes and generated knockdown mutants using CRISPR/Cas9 technology. Slripk mutants displayed reduced ROS production in response to representative immune elicitors and were susceptible to pathogenic bacteria and fungi from different genera, including Ralstonia solanacearum, Pectobacterium carotovorum, Botrytis cinerea, and Fusarium oxysporum, which are leaf and root pathogens with hemibiotrophic and necrotrophic infection strategies. In contrast, transgenic tomato plants overexpressing SlRIPK are more resistant to these pathogens. Remarkably, the slripk mutants and SlRIPK-overexpressing transgenic plants did not exhibit significant growth retardation or yield loss. These results suggest that overexpression of SlRIPK confers broad-spectrum disease resistance without a yield penalty in tomato plants. Our findings suggest that modifying ROS homeostasis by altering the regulatory components of ROS production in plant immunity could contribute to engineering or breeding broad-spectrum disease-resistant crops without yield penalty.

Fusarium Head Blight Epidemics in Soft Red Winter Wheat Fields in Georgia from 2018 to 2019

Fusarium head blight (FHB) epidemics on wheat have caused significant yield and economic penalties in the United States since the early 1990s. This report documents FHB epidemics on soft red winter wheat in Georgia in 2018 and 2019. Forty-four wheat fields across 23 counties were assessed for FHB incidence (2019 only), Fusarium-damaged kernel, deoxynivalenol (DON) contamination, and thousand-kernel weight. Higher levels of FHB were observed in 2019 compared with 2018. A significant correlation was observed between DON and 7-day preanthesis weather variables in 2019. FHB parameters were significantly correlated with postanthesis weather variables at 10 days in both years and at 20 and 30 days in 2018, suggesting that postanthesis rather than preanthesis weather had a greater impact on FHB in our study. The combination of hours of conducive temperature and relative humidity postanthesis was consistently correlated with all FHB parameters in both years and could be the best predictor of FHB epidemics. FHB has emerged as the leading threat for soft red winter wheat production in Georgia. Planting moderately resistant wheat cultivars along with in-season management, including proper fungicide application, by closely monitoring the national FHB forecasting system would be the best integrated management strategies for Georgian wheat growers.

Response of chickpea to varying moisture stress conditions in Ethiopia

AbstractChickpea (Cicer arietinum L.) is an economically important crop grown by nearly one million Ethiopian smallholder farmers. The crop is often considered as “stress‐loving,” but moisture stress at flowering and grain filling stages could be detrimental. Yield of chickpea is commonly affected by terminal drought stress in the rainfed production system in Ethiopia. The lack of proper field‐screening methods has hindered the development of drought‐tolerant varieties. This study demonstrates a simple and practical field‐level screening method for drought tolerance traits in the conventional breeding programs. A field experiment was conducted using 28 elite chickpea cultivars during the 2018–2019 main cropping season to study their response to moisture regimes of varying drought intensities. We used yield and its components as proxy parameters of screening to select tolerant cultivars. The study revealed significant variation among the cultivars in their response to different moisture regimes. The kabuli cultivars were found more sensitive compared with the desi types. Yield penalty exceeded 70% under severe drought. Conversely, cultivars tested under mild and severe stress drought showed average yield gain of 22 and 48%, respectively, relative to the irrigated treatment. Overall, over 50% yield gain can be obtained in drought‐affected rainfed production areas in Ethiopia using supplemental irrigation during pod setting to grain filling stages. For post‐rainy‐season crops relying on residual soil moisture, such as chickpea, breeding for shorter duration and resilient cultivars are reliable management approaches to minimize drought‐caused yield losses.

Microclimate control to increase productivity and nutritional quality of leafy vegetables in a cost-effective manner

Food security is one of the key global challenges in this century. In Singapore, our research team has been using novel aeroponic technology to produce fresh vegetables since 1997. Aeroponic systems allow for year-round production of not only tropical, but also sub-tropical and temperate fresh vegetables, by simply cooling the roots suspended in aeroponic systems while the aerial parts grow under tropical ambient environments. It has also been used to investigate the impacts of root-zone CO2 on vegetables by enriching root-zone CO2 while their aerial portions were subjected to constant atmospheric CO2. To compensate for the lack of available land, Singapore also needs to develop a farming system that can increase productivity per unit land area by many-fold. Over the past 10 years, my research team has established a commercially viable LED integrated vertical aeroponic farming system to grow different leafy vegetables under different LED spectra, intensities, and durations in the tropical greenhouse. The results demonstrate that it is possible to increase shoot production and rate of shoot production of leafy vegetables by increasing light intensity and extending the photoperiod under effective LED lighting. Furthermore, temperate vegetable crops such as lettuce were able to acclimate to high light intensity under supplementary LED lights to natural sunlight in the greenhouse. Supplementary LED lightings promote both leaf initiation and expansion with increased photosynthetic pigments, higher Cyt b6f and Rubisco protein contents on a per area basis and thus improve photosynthetic capacity and enhance productivity. Plants sense and respond to changes in their immediate environments (microclimate), manipulating the root zone temperature (RZT) and water supply will impact not only their growth and development but also their nutritional quality. Our on-going research aims to investigate if the nutritional quality of leafy vegetables could be improved under suboptimal RZT and mild water deficit through deficit irrigation. If substantial energy and water savings in urban farming can be achieved without substantial yield penalty but with higher nutritional quality, the amount of water and energy saved can bring substantial benefits to society. Keywords: leaf vegetables, LED lighting, microenvironment, nutrient spraying intervals, nutritional quality, photosynthetic performance, productivity, root-zone temperature DOI: 10.25165/j.ijabe.20221503.7367 Citation: He J, Qin L. Microclimate control to increase productivity and nutritional quality of leafy vegetables in a cost-effective manner. Int J Agric & Biol Eng, 2022; 15(3): 55–61.

Using knowledge-based management for sustainable phosphorus use in China

Sustainable phosphorus (P) management presents challenges in crop production and environmental protection; the current understanding of chemical P-fertilizer manufacturing, rock phosphate (RP) mining, P loss within supply chains, and strategies to mitigate loss is incomplete because of a fragmented understanding of P in the crop production supply chain. Therefore, we develop a knowledge-based management theoretical framework to analyze P supply chains to explore ways to mitigate China's P crisis. This framework connects upstream P industries and crop production, addressing knowledge gaps and stakeholder involvement. We demonstrate the potential to improve P use efficiency in the supply chain, thereby mitigating the P crisis using optimized P management. Our results showed that P footprint and grain production demand for RP can be reduced without yield penalty using a crop-demand-oriented P supply chain management that integrates P use in crop production, P-fertilizer manufacturing, and RP mining. Food security and P-related environment sustainability can be achieved by sharing responsibility and knowledge among stakeholders.

TT2 controls rice thermotolerance through SCT1-dependent alteration of wax biosynthesis.

Global warming threatens crop production. G proteins mediate plant responses to multiple abiotic stresses. Here we identified a natural quantitative trait locus, TT2 (THEROMOTOLERANCE 2), encoding a Gγ subunit, that confers thermotolerance in rice during both vegetative and reproductive growth without a yield penalty. A natural allele with loss of TT2 function was associated with greater retention of wax at high temperatures and increased thermotolerance. Mechanistically, we found that a transcription factor, SCT1 (Sensing Ca2+ Transcription factor 1), functions to decode Ca2+ through Ca2+-enhanced interaction with calmodulin and acts as a negative regulator of its target genes (for example, Wax Synthesis Regulatory 2 (OsWR2)). The calmodulin-SCT1 interaction was attenuated by reduced heat-triggered Ca2+ caused by disrupted TT2, thus explaining the observed heat-induced changes in wax content. Beyond establishing a bridge linking G protein, Ca2+ sensing and wax metabolism, our study illustrates innovative approaches for developing potentially yield-penalty-free thermotolerant crop varieties.

Profitability of dual‐purpose rye cover crop as influenced by harvesting date

AbstractHarvesting annual winter cereal rye (Secale cereale L.) (WCR) as an emergency forage could offset cover crop establishment costs, facilitate WCR adoption, and still provide multiple ecosystem benefits. A five site‐year trial was conducted in Colorado (CO) and Illinois (IL) to evaluate the effect of harvest date on WCR forage yield, quality, and its economic performance. From March to April, WCR dry matter (DM) yield increased exponentially in CO and linearly in IL. The DM yield at Julian day (DOY) 112–116 in CO was 6.9, 5.0, and 5.2 Mg ha–1 in 2018, 2019, and 2020, respectively, compared with 4.7 and 2.7 Mg ha–1 in IL in 2019 and 2020, respectively. Delayed harvesting increased acid detergent fiber and neutral detergent fiber concentrations and decreased crude, total digestible nutrients, and relative feed quality. Yield–quality trade‐off showed that forage yield increased rapidly but forage quality declined after DOY 105–108. Economic analysis, including cost of nutrient removal and 10% cash crop (corn [Zea mays L.]) yield penalty following WCR production revealed harvesting WCR biomass as forage was economically feasible in four out of five site‐years at hay price over $132 Mg–1. Eliminating corn yield penalty indicated profitability in four site‐years at hay price of ≥$110 Mg–1 and removing nutrient removal costs made all site‐years profitable at hay price of ≥$110 Mg–1. It was concluded that harvesting WCR biomass can be a profitable and effective strategy for sustainable intensification that can offer environmental stewardship and economic benefit.