Sugar Yield Research Articles

Effect of fertilizers and liming on soil condition and productivity of sugar beet

Purpose. To investigate the influence of an alternative organo-mineral fertilization system on the productivity of sugar beet at different application rates of lime. Methods. Short-term field and analytical. Results. The results of research on the influence of alternative organo-mineral fertilizers and liming on the alkaline-acidic balance of leached chernozem, its nutritional regime and productivity of sugar beets are presented. The perspective of using traditional and alternative fertilizers combined with lime on weakly acidic soil in conditions of sufficient moisture was revealed. Conclusions. The application of N90P90K90 + 5 t/ha of straw and 7.5 t/ha of defecation sludge in sugar beet ensured the maximum stability of the acid-alkaline balance of leached chernozem, increased the amount of absorbed alkalis with an increase in the content of calcium and magnesium in the soil-absorbing complex. Obtained рНsalt was 6.62, hydrolytic acidity 1.69 mg-eq/100 g, amount of absorbed alkalis 28.4 mg-eq/100 g of soil. Application of N90P90K90 + 5 t/ha of straw and 7.5 t/ha defecation sludge ensured the highest mobility of phosphorus and potassium in leached chernozem: the content of mobile phosphorus in the 0–30 cm layer was 170 mg/kg and potassium 128 mg/kg that provided increase of 32 and 38 mg/kg of soil, respectively, compared to control. Liming increased the mobility of potassium in the soil and did not affect the content of mobile phosphorus. The maximum productivity of sugar beet on leached chernozem was achieved with the application of N90P90K90 + 5 t/ha of straw + 7.5 t/ha of defecation sludge (1.5 standard rate of CaСО3). Obtained root yield was 73.3 t/ha, sugar content 18.6%, and sugar yield 13.6 t/ha that is higher compared to control without fertilizers and defecation sludge by 22.7 t/ha, 0.2%, and 4.3 t/ha, respectively.

Recovery of sulfuric acid during sugar purification from pretreated wheat straw hydrolysate using electro-electrodialysis system for 2G bioethanol production

Concentrated acid hydrolysis (CAH) of lignocellulosic biomass (LCB) is an established method to obtain a near theoretical yield of monomeric sugars from the biomass. The CAH process produced 83.23 % of glucose from pretreated wheat straw (PWS) at optimum conditions (i.e., stage I = 74 % w/w acid, 1:2 biomass loading, and 50 °C for 1 h; and stage II = 30 % w/w acid, 90 °C for 2 h). However, the separation of sugar from acidic hydrolysate is a major constraint of this technology. In this study, an electro-electrodialysis (EED) process using a novel anion exchange membrane (AEM) has been developed to recover sulfuric acid from biomass hydrolysate. The synthetic hydrolysate (i.e., feed acid concentration = 2 M; glucose = 80 g L−1) was used to optimize the EED process parameters. The acid was successfully recovered from alkali pretreated wheat straw (PWS) hydrolysate using the most effective EED process parameters. This resulted in an 82.72 % acid recovery in the permeate under the following conditions: current density of 88 mA cm−2, flow rate of 3 L h−1, and feed acid content of 2 M. During the EED process, the highest glucose recovery in the feed was 90.98 %, achieved with a maximum current efficiency of 93.57 % and the lowest energy consumption of 0.052 kW h mol−1 at optimal conditions using PWS Hydrolysate. Saccharomyces cerevisiae fermented the acid-free PWS hydrolysate, resulting in a maximum ethanol yield of 92.3 %. The EED process cost of $0.049 kg H2SO4−1 and total revenue was $0.02 kg biomass−1 from the second batch onwards suggest the feasibility of the EED process for large-scale 2G bioethanol productions.

Development of an Integrated Bioprocess System for Bioethanol and Arabitol Production from Sugar Beet Cossettes.

An innovative integrated bioprocess system for bioethanol production from raw sugar beet cossettes (SBC) and arabitol from remaining exhausted sugar beet cossettes (ESBC) was studied. This integrated three-stage bioprocess system is an example of the biorefinery concept to maximise the use of raw SBC for the production of high value-added products such as sugar alcohols and bioethanol. The first stage of the integrated bioprocess system was simultaneous sugar extraction from SBC and its alcoholic fermentation to produce bioethanol in an integrated bioreactor system (vertical column bioreactor and stirred tank bioreactor) containing a high-density suspension of yeast Saccharomyces cerevisiae (30 g/L). The second stage was the pretreatment of ESBC with dilute sulfuric acid to release fermentable sugars. The resulting liquid hydrolysate of ESBC was used in the third stage as a nutrient medium for arabitol production by non-Saccharomyces yeasts (Spathaspora passalidarum CBS 10155 and Spathaspora arborariae CBS 11463). The obtained results show that the efficiency of bioethanol production increased with increasing temperature and prolonged residence time in the integrated bioreactor system. The maximum bioethanol production efficiency (87.22 %) was observed at a time of 60 min and a temperature of 36 °C. Further increase in residence time (above 60 min) did not result in the significant increase of bioethanol production efficiency. Weak acid hydrolysis was used for ESBC pretreatment and the highest sugar yield was reached at 200 °C and residence time of 1 min. The inhibitors of the weak acid pretreatment were produced below bioprocess inhibition threshold. The use of the obtained liqiud phase of ESBC hydrolysate for the production of arabitol in the stirred tank bioreactor under constant aeration clearly showed that S. passalidarum CBS 10155 with 8.48 g/L of arabitol (YP/S=0.603 g/g and bioprocess productivity of 0.176 g/(L.h)) is a better arabitol producer than Spathaspora arborariae CBS 10155. An innovative integrated bioprocess system for the production of bioethanol and arabitol was developed based on the biorefinery concept. This three-stage bioprocess system shows great potential for maximum use of SBC as a feedstock for bioethanol and arabitol production and it could be an example of a sustainable 'zero waste' production system.

The Integrated Effect of Organic Manure, Biofertilizer and Inorganic Fertilizer on Soil Properties, Yield and Quality in Sugarcane Plant-ratoon System under Calcareous Soil of Indo-gangetic Plains of India

A field experiment was conducted at the Research Farm of Dr. Rajendra Prasad Central Agricultural University, Pusa, Bihar, to devise nutrient management strategies aimed at sustaining soil health, quality and sugarcane production within the sugarcane plant-ratoon system. The experiment evaluated the efficacy of various fertilizers on the solubility of applied inorganic fertilizer during the spring season in calcareous soil. Results from the combined data analysis showed significant variations in the number of millable cane (NMC), cane yield, and sugar yield due to the integrated application of organic and inorganic fertilizers along with bio-fertilizers in both the plant and ratoon crops. Treatments combining organic and inorganic fertilizers with bio-fertilizers demonstrated a noteworthy increase in NMC, cane yield, and sugar yield compared to the control group. The treatment that received 75% NPK of recommended dose of fertilizer (RDF) along with Acetobacter, PSB, and Bio-compost at a rate of 7.5 t ha-1 exhibited the highest NMC (103.0 × 103 ha-1), cane yield (85.8 t ha-1), and sugar yield (11.21 t ha-1). Furthermore, the residual effect of the treatment combining organic and inorganic fertilizers with bio-fertilizers showed a pronounced impact on NMC (92.4 × 103 ha-1), yield (79.6 t ha-1), and sugar yield (9.36 t ha-1) in the ratoon crop under treatment T9. Bio-compost notably enhanced the overall performance of sugarcane. Nutrient uptake by both plant and ratoon followed a similar trend as cane yield. Application of RDF alongside various bio-fertilizers significantly enhanced sugarcane productivity compared to the control group. Notably, the efficacy of bio-fertilizers was more pronounced when used in conjunction with inorganic treatments. Treatment with organic inputs through bio-compost resulted in reduced pH and increased electrical conductivity (EC), organic carbon, and available nutrients (N,P, and K) in post-harvest soil. Enzymatic activities including glycosidase, urease, acid phosphatase, and dehydrogenase were also recorded.

Enhancing biomass conservation and enzymatic hydrolysis of sweet sorghum bagasse by combining pretreatment with ensiling and NaOH.

Lignocellulosic pretreatment is an important stage in biomass utilization, which usually requires high input. In this study, a low-cost method using combined ensiling and NaOH was developed for lignocellulosic pretreatment. Sweet sorghum bagasse (SSB) was ensiled for 21 days and then treated with diluted NaOH (0%, 1%, and 2%) for fermentation. The results showed that the application of Lactobacillus plantarum (L) reduced fermentation losses of the silages, mainly low water-soluble carbohydrate (WSC) and ammonia nitrogen loss. Meanwhile, the application of Lactobacillus plantarum and ensiling enzyme (LE) promoted lignocellulosic degradation, as evidenced by low neutral detergent fiber (NDF), acid detergent fiber (ADF), lignin (ADL), and hemicellulosic (HC) contents. The dominant bacterial genera were Lactobacillus, uncultured_bacterium_f_Enterobacteriaceae, and Pantoea after silage, which corresponded to the higher lactic acid and acetic contents and lower pH. The reducing sugar yields of SSB increased after combined pretreatment of silage and NaOH and were further enhanced by the 2% NaOH application, as evidenced by the high reducing sugar yield and microstructure damage, especially in the L-2% NaOH group and the LE-2% NaOH group, in which the reducing sugar yields were 87.99 and 94.45%, respectively, compared with those of the no additive control (CK)-0 NaOH group. Therefore, this study provides an effective method for SSB pretreatment to enhance biomass conservation.

Spatiotemporal Trends of the Carbon Footprint of Sugar Production in China

Sugar is the main nutrient source of human beings and the basic raw material of the food industry. It is of great practical significance for China to find out the current situation and future trend of Greenhouse Gas (GHG) emissions from sugar production, and explore measures for reducing GHG emissions from sugar planting and processing. The IPCC GHG emissions accounting method is applied to analyze the carbon footprint of sugar production based on planting management and industrial development data, in which the GHG emissions per unit of sugar product are calculated and analyzed. According to Carbon Peak and Carbon Neutrality targets, the total GHG emissions of China's sugar industry in 2030 and 2060 are predicted and discussed. The results show that: (1) The average GHG emissions per unit yield of sugar show a downward trend from 2004 to 2021. The total GHG emissions of sugar production in China have reached 1319.9 × 104 t CO2eq in 2021, which has not yet reached its peak due to the growth of consumption demand. (2) The average GHG emissions per unit of Beet Sugar (1.477 tCO2eq/t) is higher than that of Cane Sugar (0.825 tCO2eq/t). It is more meaningful to enlarge the planting area of sugarcane than that of sugarbeet in the future. (3) Overall, in 2021 the GHG emissions of sugar production reached 0.91 tCO2eq/t in China, which would decline 15 %–87 % until 2030 and 2060. There is great potential for sugar production to reduce GHG emissions in the future.

Optimisation of sugar and solid biofuel co-production from almond tree prunings by acid pretreatment and enzymatic hydrolysis.

Almond pruning biomass is an important agricultural residue that has been scarcely studied for the co-production of sugars and solid biofuels. In this work, the production of monosaccharides from almond prunings was optimised by a two-step process scheme: pretreatment with dilute sulphuric acid (0.025M, at 185.9-214.1℃ for 0.8-9.2min) followed by enzyme saccharification of the pretreated cellulose. The application of a response surface methodology enabled the mathematical modelling of the process, establishing pretreatment conditions to maximise both the amount of sugar in the acid prehydrolysate (23.4kg/100kg raw material, at 195.7℃ for 3.5min) and the enzymatic digestibility of the pretreated cellulose (45.4%, at 210.0℃ for 8.0min). The highest overall sugar yield (36.8kg/100kg raw material, equivalent to 64.3% of all sugars in the feedstock) was obtained with a pretreatment carried out at 197.0℃ for 4.0min. Under these conditions, moreover, the final solids showed better properties for thermochemical utilisation (22.0MJ/kg heating value, 0.87% ash content, and 72.1mg/g moisture adsorption capacity) compared to those of the original prunings.

Improving saccharification efficiency of corn stover through ferric chloride-deep eutectic solvent pretreatment

An effective deep eutectic solvent (DES) and Iron(III) chloride (FeCl3) combination pretreatment system was developed to improve the removal efficiency of lignin and hemicellulose from corn stover (CS) and enhance its saccharification. N-(2-hydroxyethyl)ethylenediamine (NE) was selected as the hydrogen-bond-donor for preparing ChCl-based DES (ChCl:NE), and a mixture of ChCl:NE (60 wt%) and FeCl3 (0.5 wt%) was utilized for combination pretreatment of CS at 110 ℃ for 50 min. FeCl3/ChCl:NE effectively removed lignin (87.0 %) and xylan (55.9 %) and the enzymatic hydrolysis activity of FeCl3/ChCl:NE-treated CS was 5.5 times that of CS. The reducing sugar yield of pretreated CS was 98.6 %. FeCl3/ChCl:NE significantly disrupted the crystal structure of cellulose in CS and improved the removal of lignin and hemicellulose, enhancing the conversion of cellulose and hemicellulose into monomeric sugars. Overall, this combination of FeCl3 and DES pretreatment methods has high application potential for the biological refining of lignocellulose.

Advancing safe bioenergy production from cadmium-contaminated sweet sorghum through mild synergistic pretreatment

Phytoremediation is an eco-friendly and cost-effective method for treating cadmium (Cd)-contaminated soil. However, safe disposal of harvested hyperaccumulators remains scarce. Herein, a mild synergistic pretreatment combining dilute acid and alkali pretreatment was developed for safe biorefining of the Cd-containing sweet sorghum, and Cd was centrally extracted into the acid pretreatment liquid in the first step. The results showed that the synergistic pretreatment was more effective in removing Cd from the biomass and overcoming biomass recalcitrance. During the synergistic pretreatment, the Cd concentration in biomass dramatically decreased from 4.03 to 0.00626 mg/kg, and a considerable amount of lignin and hemicelluloses in biomass was removed, facilitating enzymatic saccharification and safe bioenergy production. The optimal conditions of the synergistic pretreatment resulted in a total sugar yield of 81.6 wt %, which was 4.7-fold higher than that of the original biomass. This strategy holds promise for reducing the cost of industrial saccharification and is a valuable inspiration for the safe handling and resource utilization of Cd-containing biomass.

Bioethanol Production from Characterized Pre-treated Sugarcane Trash and Jatropha Agrowastes

Low production costs and a potential feedstock supply make lignocellulosic ethanol (bioethanol) an important source of advanced biofuels. The physical and chemical preparation of this kind of lignocellulosic feedstock led to a high ethanol yield. In order to increase the yield of fermentable sugars, pretreatment is an essential process step that alters the lignocellulosic structure and improves its accessibility for the expensive hydrolytic enzymes. In this context, the chemical composition of sugarcane trash (dry leaves, green leaves, and tops) and jatropha (shell and seed cake) was determined to be mainly cellulose, hemicellulose, and lignin. Hydrogen peroxide and sodium hydroxide were applied in an attempt to facilitate the solubilization of lignin and hemicelluloses in five agrowastes. The extraction of hydrogen peroxide was much better than that of sodium hydroxide. A comparative study was done using SEM, EDXA, and FTIR to evaluate the difference between the two methods. The pretreated wastes were subjected to saccharification by commercial cellulases (30 IU/g substrate). The obtained glucose was fortified with nutrients and fermented statically by Saccharomyces cerevisiae F-307 for bioethanol production. The results revealed the bioethanol yields were 325.4, 310.8, 282.9, 302.4 and 264.0 mg ethanol/g treated agrowastes from green leaves of sugarcane, jatropha deolied seed cake, tops sugarcane, dry leaves of sugarcane, and jatropha shell, respectively. This study emphasizes the value of lignocellulosic agricultural waste as a resource for the production of biofuels as well as the significance of the extraction process.

Yield, Quality and Disease Resistance of Sugarcane Clones: A Field Evaluation

Evaluation and identification of sugarcane clones for different maturity groups is of paramount importance in sugarcane cultivation to get higher recoveries in sugar mills. A field experiment was conducted to evaluate the performance of early maturing sugarcane clones for red rot resistance, cane yield, CCS yield, and their contributing traits in order to idendify the superior clones. Observation on germination per cent, number of tillers (x1000/ha), number of millable cane (x1000/ha), stalk length (cm), stalk diameter (cm), single cane weight (kg), cane yield (t/ha), brix per cent, sucrose (%), purity (%), extraction CCS (%), and sugar yield (t/ha). From the results, it could be concluded that the early maturing clone, CoC 15336, was found to be the best among the test clones for sucrose per cent and sugar yield with resistance to red rot disease. Another clone, CoC 15340, was the next-best entry, with higher cane yield, CCS yield, and sucrose percent compared to the better standards. As a result, clones CoC 15338 and CoC 15336 were identified as the best promising entries and could be forwarded for further yield evaluation trials for release as a new sugarcane variety suitable for East Coast Zone of India.

Enhancement of cellulolytic enzyme production from intrageneric protoplast fusion of Aspergillus species and evaluating the hydrolysate scavenging activity

BackgroundLignocellulosic biomass provides a great starting point for the production of energy, chemicals, and fuels. The major component of lignocellulosic biomass is cellulose, the employment of highly effective enzymatic cocktails, which can be produced by a variety of microorganisms including species of the genus Aspergillus, is necessary for its utilization in a more productive manner. In this regard, molecular biology techniques should be utilized to promote the economics of enzyme production, whereas strategies like protoplast fusion could be employed to improve the efficacy of the hydrolytic process.ResultsThe current study focuses on cellulase production in Aspergillus species using intrageneric protoplast fusion, statistical optimization of growth parameters, and determination of antioxidant activity of fermentation hydrolysate. Protoplast fusion was conducted between A. flavus X A. terreus (PFFT), A. nidulans X A. tamarii (PFNT) and A. oryzae X A. tubingensis (PFOT), and the resultant fusant PFNT revealed higher activity level compared with the other fusants. Thus, this study aimed to optimize lignocellulosic wastes-based medium for cellulase production by Aspergillus spp. fusant (PFNT) and studying the antioxidant effect of fermentation hydrolysate. The experimental strategy Plackett-Burman (PBD) was used to assess how culture conditions affected cellulase output, the best level of the three major variables namely, SCB, pH, and incubation temperature were then determined using Box-Behnken design (BBD). Consequently, by utilizing an optimized medium instead of a basal medium, cellulase activity increased from 3.11 U/ml to 7.689 U/ml CMCase. The following medium composition was thought to be ideal based on this optimization: sugarcane bagasse (SCB), 6.82 gm; wheat bran (WB), 4; Moisture, 80%; pH, 4; inoculum size, (3 × 106 spores/ml); and incubation Temp. 31.8 °C for 4 days and the fermentation hydrolysate has 28.13% scavenging activities.ConclusionThe results obtained in this study demonstrated the significant activity of the selected fusant and the higher sugar yield from cellulose hydrolysis over its parental strains, suggesting the possibility of enhancing cellulase activity by protoplast fusion using an experimental strategy and the fermentation hydrolysate showed antioxidant activity.

Influence of High-Sugar Early and Mid-Late Genotypes on Yield and Quality of Sugarcane (Saccharum spp. Hybrid Complex) at Wider Row Spacing

A field experiment was conducted in Pusa during spring season of 2016-17, to evaluate 7 sugarcane genotypes (‘CoP 11436’, ‘CoP 11437’, ‘CoP 11438’, ‘CoSe 11451’, ‘BO 130’, ‘CoSe 95422’ and ‘BO 153’) in early maturing group and 7 sugarcane genotypes viz., ‘BO 155’, ‘CoSe 11453’, ‘CoSe 11454’, ‘CoSe 11455’, ‘BO 91’, ‘CoP 9301’ and ‘CoSe 92423’ in mid late maturing group at 120 cm row spacing. In early group, genotype ‘CoP 11436’ produced the higher tillers (1, 38000/ha), millable canes (1, 04800/ ha), cane (88.2 t/ha) and sugar yield (11.1 t/ ha). Genotype, ‘CoP 11438’ showed the higher brix (20.6) and pol (18.39%) per cent juice among test genotype, followed by standard check ‘BO 153’. Under mid late maturing group, higher germination count (46.2%), tillers (1, 52, 100/ha) and millable canes (1, 07800/ ha) was noticed due to the genotype ‘BO 155’ which was followed by the standard check ‘CoSe 92423’. However, ‘CoSe 11455’ recorded the highest cane (103.7 t/ha) and sugar yield (13.18 t/ha), followed by ‘BO 155’ in case of cane yield and CoSe 11453 in sugar yield. Among mid late genotype ‘CoSe 11453’, ‘CoSe 11454’ and standard check ‘CoP 9301’ obtained similar brix values of 20.7%. However, standard check ‘CoP 9301’ was found significantly superior to ‘BO 155’ with respect to pol and commercial cane sugar percent.

Improving sugarcane agronomy: Field evidence for ultrasonic treatment enhancing yield, growth, and physiological and biochemical characteristics

Ultrasonic treatment (UT) is a safe and efficient physical stimulus used to improve crop germination and growth. However, previous studies have concentrated on the germination and seedling stages of ultrasonically treated crops, while field tests of the effects of ultrasound on sugarcane growth have not been reported. In this study, we compared the growth, photosynthetic characteristics, and antioxidant properties of three sugarcane varieties, ‘ROC22’, ‘LC05–136’, and ‘YT93–159’, under no UT (T1), UT (20–40 kHz mixed frequency, dry treatment) for 1 min (T2), UT for 2 min (T3), and UT for 5 min (T4). The field experiments were conducted from January 2021 to January 2023.The effect of UT on newly-planted sugarcane was greater than on ratoon sugarcane for all measured parameters in both years. Compared with the T1 treatment, in 2021 the increases in cane yield and sugar yield under UT (T3 and T4) were 9.76%–28.57% and 7.80%–21.21%, respectively, while in 2022, the increases were 1.19%–12.64% and 2.16%–8.39%, respectively. From the two-year data, compared with the T1 treatment, the sucrose content of sugarcane under the T3 treatment in V1 increased by 0.38%–15.26%; the T3 and T4 treatments in V2 increased the sucrose content by 0.48%–4.90%, and the T4 treatment in V3 increased the content by 1.17%–5.85%. Moreover, suitable UT increased plant height, stem diameter, root length, and germination rate. Suitable UT also increased the chlorophyll content, net photosynthetic rate, stomatal conductance, transpiration rate, superoxide dismutase activity, peroxidase activity, catalase activity, soluble protein content, and soluble sugar content while decreasing the malondialdehyde content in sugarcane leaves. Meanwhile, a multi-analysis showed that a more developed root system, a more efficient photosynthetic rate, and better antioxidant properties after UT treatment led to increased sugar yield of sugarcane. This study is the first to demonstrate that suitable UT (2–5 min, 20–40 kHz mixed frequency, dry treatment) can significantly increase the yield and quality of sugarcane based on field tests. Furthermore, this study shows that ultrasonic seed treatment technology has promising applications in sugarcane cultivation and significant potential in agricultural production.

Comparative Analysis of Acidic and Alkaline Pretreatment Techniques for Bioethanol Production from Perennial Grasses

This review paper examines acid and alkaline pretreatments on perennial grasses for second-generation (2G) bioethanol production, a relatively unexplored area in this field. It compares the efficiency of these pretreatments in producing fermentable sugar and bioethanol yield. This study finds that alkaline pretreatment is more effective than acidic pretreatment in removing lignin and increasing sugar yield, leading to higher ethanol yields. However, it is costlier and requires longer reaction times than acidic pretreatment, while acidic pretreatment often leads to the formation of inhibitory compounds at higher temperatures, which is undesirable. The economic and environmental impacts of lignocellulosic biomass (LCB) are also assessed. It is revealed that LCB has a lower carbon but higher water footprint and significant costs due to pretreatment compared to first-generation biofuels. This review further explores artificial intelligence (AI) and advanced technologies in optimizing bioethanol production and identified the gap in literature regarding their application to pretreatment of perennial grasses. This review concludes that although perennial grasses hold promise for 2G bioethanol, the high costs and environmental challenges associated with LCB necessitate further research. This research should focus on integrating AI to optimize the pretreatment of LCB, thereby improving efficiency and sustainability in 2G biofuel production.

Production of sugars from lignocellulosic biomass via biochemical and thermochemical routes

Sugars are precursors to the majority of the world’s biofuels. Most of these come from sugar and starch crops, such as sugarcane and corn grain. Lignocellulosic sugars, although more challenging to extract from biomass, represent a large, untapped, opportunity. In response to the increasing attention to renewable energy, fuels, and chemicals, we review and compare two strategies for extracting sugars from lignocellulosic biomass: biochemical and thermochemical processing. Biochemical processing based on enzymatic hydrolysis has high sugar yield but is relatively slow. Thermochemical processing, which includes fast pyrolysis and solvent liquefaction, offers increased throughput and operability at the expense of low sugar yields.

Pretreatments Applied to Wheat Straw to Obtain Bioethanol

This work is a comprehensive study focusing on various methods for processing wheat straw to enhance its suitability for bioethanol production. It delves into mechanical, physical, chemical, and biological pretreatments, each aimed at improving the enzymatic hydrolysis and fermentation processes necessary for bioethanol production. Mechanical and physical pretreatments involve reducing the size of wheat straw to improve enzymatic hydrolysis. Physical methods include heating and irradiation, which alter the structural properties of wheat straw. Chemical pretreatments involve using acids, alkalis, and organic solvents to remove lignin and hemicellulose, making cellulose more accessible for hydrolysis. Biological pretreatments utilize microorganisms and fungi to degrade lignin and other complex compounds, enhancing the breakdown of cellulose. The study presents data on the effectiveness of these treatments in terms of lignin removal, sugar yield, and overall bioethanol production efficiency. The research is aligned with the global move towards renewable energy sources and emphasizes the importance of utilizing agricultural waste, like wheat straw, for sustainable energy production.

Enzymatic hydrolysates of Agave tequilana bagasse pretreated with protic ionic liquids: Saccharification efficiency and hydrogen production

This work demonstrated for the first time that hydrogen (H2) can be generated from Agave tequilana bagasse (ATB) after ionic liquid (IL) pretreatment up to 111.1 L H2/kg hydrolyzed biomass in only 20 h. It is shown that the low-cost protic IL [EOA][OAc] (ethanolamine acetate) can effectively pretreat ATB at two different scales (bench and lab) using 2 and 250 g (dry basis) at 20% solids loading and 160 °C for 90 min resulting in significant structural changes followed by FTIR spectroscopy. The lignin content of the pretreated ATB was reduced by 28.6% after lab-scale pretreatment which ultimately improved the sugar yield with the different saccharification strategies (batch vs. fed-batch). Up to 0.643 g TS (total sugars)/g ATB were obtained in a fed-batch strategy with fixed enzymatic loading, representing an enhancing 2.8-fold when compared to the untreated biomass. Models of enzymatic saccharifications were included for both, untreated and pretreated ATB, in terms of TS concentration (g/L) and fiber length (mm) to confirm differences in accessibility and availability of both substrates. The results successfully revealed that 1.1 kg or 14 m3 of H2 can be produced from 100 kg of dry ATB after pretreatment with [EOA][OAc] indicating the feasibility of improved sugar and H2 yields where either a low-cost pretreatment agent or an adequate saccharification strategy contributes to the biorefinery scheme.

Coupling chemical modification and immobilization of cellulase improves thermal stability and low-melting mixture solvent tolerance for in situ saccharification of bagasse

In situ saccharification of lignocellulosic biomass in low-melting mixture solvents (LoMMSs) is a promising approach for biorefinery. In this study, chemical modification of cellulase coupled with immobilization was designed to improve thermal stability and LoMMS tolerance of cellulase for in situ saccharification. Cellulase was firstly chemically modified with succinic anhydride and ethylenediamine respectively to form succinylated cellulase (S-Ce) and aminated cellulase (A-Ce), then the modified cellulases were immobilized with amino-functionalized metal-organic frameworks to establish ZIF-8/A immobilized A-Ce and S-Ce. Circular dichroism, fluorescence measurements, X-ray diffraction, fourier transform infrared spectra and scanning electron microscopy were applied to characterize the modified cellulase or immobilization carrier. ZIF-8/A immobilized A-Ce presented desired thermal stability and LoMMS tolerance. Reducing sugar yields via ZIF-8/A immobilized A-Ce during in situ saccharification of bagasse maintained stably till Bet/AA concentration reached 50%, and declined relatively slowly compared with free or other immobilized cellulase when Bet/AA concentration further increased to 90%. In situ saccharification of bagasse via ZIF-8/A immobilized A-Ce in concentrated Bet/AA (50%) can be operated at a wide temperature range (50–60 °C). The corresponding reducing sugar yield with ZIF-8/A immobilized A-Ce was 58% higher than that with free cellulase in 50% Bet/AA at 60 °C.

Sugar beet growth and yield as influenced by irrigation source as well as fertilization

Sugar beet growth and yield as influenced by irrigation source as well as fertilization