Yellow Plates Research Articles

A fatal case of Harlequin ichthyosis: Experience from low-resource setting.

Harlequin ichthyosis is a severe and fatal presentation of ichthyosis with an autosomal recessive inheritance. Infants with Harlequin ichthyosis have a high mortality rate, and a dismal prognosis; therefore the majority of neonates die shortly after birth from infection, heat loss, dehydration, electrolytic imbalances, or respiratory distress. The aim of this case report was to present a fatal case of Harlequin ichthyosis with no family history of any inherited skin disorder. A 3-day-old baby was presented to the emergency room with congenital abnormalities at birth, fissured hyperkeratotic skin, and thick yellow plates of scales. The parents had no history of consanguineous marriage, no relevant past medical history, and no family history of the same condition. The patient was unwell, pulse 162 times/minute, respiratory rate 48 times/minute, and axillary temperature 36.9oC. APGAR score was 8 in the 1st minute and 9 in the 5th minute. Based on the typical clinical appearance, the patient was diagnosed with Harlequin ichthyosis. Due to a lack of facility, a mutation analysis was not carried out. The patient was then transferred to the neonatal intensive care unit (NICU) and treated in a humidified incubator and medicated with intravenous antibiotics (ampicillin sulbactam 125 mg/12 hour and gentamicin 13 mg/24 hour), topically fusidic acid and mild emollients. A central venous catheter was used for intravenous access. The poor prognosis resulted in the patient dying at the age of 5-day-old. This case highlights that prenatal diagnosis is critical for early detection and disease prevention. Mutation screening for the ABCA12 gene is suggested for consanguinity marriages and with a history of ichthyosis.

Survey of dermestid beetles using UV-light traps in two food industry facilities in the Czech Republic: One year field study

There is a growing interest in stored product Dermestidae species due to their capacity to damage and contaminate stored products of animal and vegetable origin. Limited published information is available on surveys of these pests in museums, grain commodity storage facilities, and mills, with a noticeable absence of surveys in the food industry. Notably, information on dermestid monitoring using a colour or UV light sticky traps, which are required as part of international food safety standards and ISO pest control procedures for food facilities, is lacking. This study represents the first report on dermestid surveys conducted in a beverage and a food packaging facility premises using traps that combine UV light with yellow sticky plates in central Europe. A total of 1031 dermestid beetle individuals were captured in the two facilities during a one-year survey period. During the sampling period, there was a seasonal trend with the maximum captured individuals from spring to early autumn, and no individuals during winter. These population fluctuations followed a similar pattern in the two facilities. In total, 13 species belonging to five genera were identified. The highest cumulative captures were found for Reesa vespulae and Anthrenus verbasci found in both facilities, followed by Trogoderma glabrum, which occurred in high numbers in the food packaging facility. Our data indicate that R. vespulae (an invasive species) is attracted to UV light + yellow traps, and its high frequency and abundance suggest that since its first isolated detection in the Czech Republic in 1987, it has proven to be a significantly spreading pest species.

Entomological diversity associated with tomato cultivation under organic shelter in the El-Outaya Region, Biskra (Algeria)

"The aim of the present work is to determine harmful and beneficial insects associated with tomato greenhouse cultivation in the arid region of El-Outaya (Biskra, Algeria). Insect trapping was conducted using yellow water plates and yellow glue traps. Faunistic data have revealed the presence of 2754 individuals distributed on seven orders, 33 families and 38 species of insects. From specific point of view, the majority of these insects were represented by potential insect pests with sixteen phytophagous species amongst them three species attacking tomatoes. Whereas nine species are predators and two pollinators. Our results can be used to plan strategies for the management of harmful pests and beneficial insects associated with the tomato greenhouse crops in this locality. Keywords: Entomofauna, inventory, tomato, greenhouse, arid."

Identifikasi dan Uji Penangkapan Radikal 2,2 Difenil-1-Pkrilhidrazil (DPPH) dari Ekstrak Kasar Pigmen Bakteri Simbion Rumput Laut

Abstrak Seaweed belongs to the group of natural antioxidants, these antioxidants are able to counteract free radicals. Secondary metabolites that are antioxidants include alkaloids, flavonoids, phenolic compounds, steroids, and terpenoids and carotenoids. This study aims to identify and test the radical scavenging of 2,2 diphenyl-1-pkrylhydrazyl (DPPH) from the crude extract of the macroalgae symbiont pigment Turbinaria conoides originating from the coastal waters of Hamadi Jayapura, Papua. The method used in this study is an experimental method by identifying pigments using Thin Chromatography (TLC), and for Antioxidant testing using the DPPH Method. The results of the study used the TLC plate Spot 1 on the yellow TLC plate, which had a Retardation frequency (Rf) value of 5/8 = 0.62 and was identified as a carotenoid. The pigment extract of the TC.11 symbiont bacterial pigment extract also has antioxidant potential which was tested by the DPPH method. See the IC50 value of TC.11 bacteria of 24315 ppm compared to the β-carotene marker of 4103 ppm

The Borderlands

The Borderlands

Structural diversity of copper(I)-ethylene complexes with 2,4-bis(2-pyridyl)pyrimidine directed by anions.

The 3 : 1 reaction of [Cu(C2H4)n]ClO4 with 2,4-bis(2-pyridyl)pyrimidine (bpprd) in Me2CO under C2H4 afforded yellow prism crystals of the dinuclear Cu(I)-C2H4 complex [Cu2(bpprd)(η2-C2H4)2(ClO4)2] (1). The 3 : 1 reaction of [Cu(C2H4)n]NO3 with bpprd in Me2CO under C2H4 afforded yellow plate crystals of the tetranuclear Cu(I)-C2H4 complex [Cu4(bpprd)2(η2-C2H4)4(μ-NO3)2](NO3)2 (2). The 10 : 1 reaction of [Cu(C2H4)n]BF4 with bpprd in Me2CO under C2H4 afforded yellow plate crystals of the dinuclear Cu(I)-C2H4 complex [Cu2(bpprd)(η2-C2H4)2(BF4)]BF4 (3). The 3 : 1 reaction of [Cu(C2H4)n]BF4 with bpprd in Me2CO under C2H4 afforded red prism crystals of the polymeric Cu(I)-C2H4 complex {[Cu6(bpprd)4(η2-C2H4)2(μ-η2:η2-C2H4)(μ-BF4)2](BF4)4}n (4). The X-ray crystal structures of complexes 1-4 have been determined. The structural diversity of Cu(I)-C2H4 complexes bridged by bpprd with different anions was demonstrated. The 1D Cu(I)-bpprd/C2H4 coordination polymer 4 bridged by unusual μ-η2:η2-C2H4 and the μ-BF4- anion is of particular significance. Complex 1 exhibited relatively well-resolved 1H NMR signals of bpprd and C2H4 (δ = 4.97 ppm) in (CD3)2CO at 23 °C.

Population Dynamics of Arthropods in New Caledonian Citrus Orchards

Aims: Agroecological infrastructures are central to the preservation of arthropod populations and ecosystem services associated. There is little data on the population dynamics of New Caledonian citrus orchards. Thus, this study focuses on the response of arthropods to four management methods in the orchard: conventional management, inter-row vegetated, row mulching and edge windbreaks.
 Place and Duration of Study: This study was conducted in the village of La Foa in New Caledonia. It took place over three months, from the end of February to the end of May 2022.
 Methodology: To collect the greatest possible diversity of insects, four complementary trapping methods were used: pitfall traps, yellow plates, Malaise traps and sweeping nets. Once collected, the trapped insects are then sorted and identified. The sorting is done with a binocular magnifying glass. Insects are identified using specific determination keys, documentation, or with the help of the Reference Collection of Terrestrial Invertebrates of New Caledonia - Xavier Montrouzier (CXMNC). When identification is not immediately possible, a morphotype number is assigned. For each species and morphospecies, a photograph is taken. Alpha and beta diversity was studied using rarefactions, Shannon index and Generalized Linear Models.
 Results: A positive effect of agroecological infrastructures on insects’ abundance and diversity was recorded. In contrast, low arthropod abundances and diversities were presented in the orchard under conventional management (P < 0.001). Mulching management was the parameter showing the much higher insects’ abundance. Moreover, populations dynamics were generally correlated with the related infrastructures. Thus, windbreaks acted as ecological corridors and the inter-row vegetation as refuge habitats for beneficial insects. 
 Conclusion: These results demonstrate the damage of conventional agriculture on arthropod diversity in New Caledonian orchards. They also show that agroecological infrastructures can bring back insect biodiversity, especially with the mulching management.

Structural Optimization of Vertically-Stacked White LEDs with a Yellow Phosphor Plate and a Red Quantum-Dot Film.

A remote-type white light-emitting diode (LED) consisting of a red quantum-dot (QD) film and a yellow phosphor plate was studied by both experiment and optical simulation. The sequence of the two color-conversion films had a substantial effect on the color-rendering properties of the vertically-stacked white LED, and the optimized configuration exhibited a high color rendering index of more than 90 thanks to the enhanced red component via the QD film. For the design of high-power white LED devices of a remote type, it was necessary to locate the color-conversion films below the diffuser plate to remove the substantial color dispersion depending on the viewing angle. The present study shows that high power and high color-rendering white LED devices can be realized in terms of two vertically-stacked color-conversion materials, which would provide long-term stability due to the remote design.

Mitochondrial Genome Architecture and Evolutionary Origin of the Yao Silkworm, a Living Fossil of the Domestic Silkworm Bombyx mori (Lepidoptera: Bombycidae).

The Yao silkworm is a unique silkworm resource producing yellow flat plate silk that has only been reared by the Baiku Yao ethnic group in Nandan County, Guangxi Province, China for a thousand years. Here, we report the mitochondrial genomes (mitogenomes) of five Yao silkworm strains and 10 local Guangxi strains of the domestic silkworm (Bombyx mori) L. (Lepidoptera: Bombycidae), and use the resulting mitogenomes and the available Bombyx mitogenomes to characterize their genome architecture and trace the evolutionary origin of the Yao silkworm. The five Yao silkworm mitogenomes exhibited genome architectures identical to typical set of 37 mitochondrial genes (13 protein-coding genes, 22 transfer RNAs, and two ribosomal RNAs) and a high level of genome sequence similarity with the domestic silkworm. Mitogenome-based phylogenetic reconstruction provided solid evidence that the Yao silkworm shares a common ancestor with the domestic silkworm. Sliding window analysis uncovered a distinct variation pattern in the mitogenome between the Yao silkworm and the other domestic silkworm strains. The phylogenetic analyses revealed a basal placement of the Yao silkworm among all available domestic silkworm strains, indicating that the Yao silkworm is an ancient population of the domestic silkworm. Our data indicated that the Yao silkworm (B. mori) is a lineage of the domestic silkworm, which for the first time provides insights into the origin of the Yao silkworm.

Populações de insetos-praga: diversidade e similaridade em cultura agrícola

The anthropic actions expressed by conventional-modern agriculture greatly alter the environment, interfering with the balance of natural processes and making its recovery mechanisms precarious. Thus, the interest in the use of microorganisms in agricultural practices has increased significantly in recent years, both in promoting plant growth and in the biological control of plant pests and diseases. The objective of the present work was to identify the insect pest population in the cucumber crop after foliar application of effective microorganisms (EM). The experiment was carried out in a rural family farm located in the city of São Sebastião-AL. Yellow (4) and blue (4) adhesive plates were used in a usable area of ​​128.8 m2. EM was diluted at a concentration of 4.7% and the applied dose was 50 mL/plant. Six collections were carried out, from December 2020 to February 2021. The individuals sampled were screened and identified at the level of class, order, family and when identified as a pest, at the level of gender. A total of 1301 invertebrate individuals of the Insecta and Arachnida classes were collected. Of these, 55.11% were identified as pest insects and 44.8% as visiting arthropods. The taxon that presented the highest relative frequency was the order Diptera (30.6%) followed by the orders Hemiptera (25.4%), Hymenoptera (24%), Coleoptera (13%), Lepidoptera (5.7%), Araneae (0.8%) and Odonata (0.5%). The pests were representatives of the genera Diabrotica spp, Anastrepha spp, Myzus spp, Bemisia spp, Atta spp and Diaphania spp. 24.7% of the visiting arthropods were predators. The arthropod population increase occurred from the 40th day after planting, with growth peak at the 57th day.

A RARE CASE OF HARLEQUIN ICHTHYOSIS SUCCESSFULLY TREATED WITH ACITRETIN: A CASE REPORT AND LITERATURE REVIEW

Harlequin Ichthyosis is the most serious congenital keratinization disorder. When the children are born, they are enveloped in thick horn armor. They are thick yellow horn plates that tear deeply when they dry out. In the most severe form, the children often die in the first few weeks of life. But there are also many milder courses, whereby there are obviously flowing transitions from collodion baby to harlequin ichthyosis. The skin condition later corresponds to that of a child with severe congenital ichthyosis (ARCI). Similar to the collodion baby, cases of harlequin ichthyosis should initially be cared for in the intensive care unit for newborns and require interdisciplinary therapy. Harlequin ichthyosis is caused by very special mutations in the ABCA12 gene. These mutations also have an impact on survival. If homozygous mutations are present, the prospects are worse than if the parents have heterozygous mutations. Homozygous mutations are often present when the parents are consanguineous.

Ichthyosis: case report in a Colombian man with genetic alterations in ABCA12 and HRNR genes

BackgroundIchthyosis is a heterogeneous group of diseases caused by genetic disorders related to skin formation. They are characterized by generalized dry skin, scaling, hyperkeratosis and frequently associated with erythroderma. Among its different types, harlequin ichthyosis (HI) stands out due to its severity. HI is caused by mutations in the ABCA12 gene, which encodes essential proteins in epidermal lipid transport, and it helps maintain the homeostasis of the stratum corneum of the epidermis. However, due to the wide spectrum of genetic alterations that can cause ichthyosis, holistic medical care, and genetic studies are required to improve the diagnosis and outcomes of these diseases.Case presentationHere, we presented the case of a 19 years old male patient who was a premature infant and exhibited clinical features consistent with HI, including bright yellow hyperkeratotic plates with erythematous fissures that covered his entire body like a collodion baby. Currently, he exhibited erythroderma, photosensitivity, ectropion, auricular pavilion alterations, and musculoskeletal disorders, such as equinovarus feet, fingers, hands, and hypoplastic feet with contractures in flexion and marked difficulty in fine motor skills. In addition, he presented dyschromatopsia, Achilles reflex hyporeflexia, slight speech, dental alteration and deficient cognitive performance. After the genetic sequencing, variants were found in ABCA12 and HRNR which are related to several skin diseases, including ichthyosis.ConclusionsAlthough in clinical practice, ichthyosis is a common entity, a severe type of ichthyosis is presented, highlighting the importance of appropriate genetic diagnosis, given the broad spectrum of genetic alterations with similar phenotypic and clinical characteristics. These pathologies must be known to guarantee initial support measures to prevent complications and offer multidisciplinary management to those patients.

Not just shades of grey: life is full of colour for the ocellate river stingray (Potamotrygon motoro).

Previous studies have shown that marine stingrays have the anatomical and physiological basis for colour vision, with cone spectral sensitivity in the blue to green range of the visible spectrum. Behavioural studies on Glaucostegus typus also showed that blue and grey can be perceived and discriminated. The present study is the first to assess visual opsin genetics in the ocellate river stingray (Potamotrygon motoro) and test whether individuals perceive colour in two alternative forced choice experiments. Retinal transcriptome profiling using RNA-Seq and quantification demonstrated the presence of lws and rh2 cone opsin genes and a highly expressed single rod (rh1) opsin gene. Spectral tuning analysis predicted these vitamin A1-based visual photopigments to exhibit spectral absorbance maxima at 461 nm (rh2), 496 nm (rh1) and 555 nm (lws); suggesting the presence of dichromacy in this species. Indeed, P. motoro demonstrates the potential to be equally sensitive to wavelengths from 380 to 600 nm of the visible spectrum. Behavioural results showed that red and green plates, as well as blue and yellow plates, were readily discriminated based on colour; however, brightness differences also played a part in the discrimination of blue and yellow. Red hues of different brightness were distinguished significantly above chance level from one another. In conclusion, the genetic and behavioural results support prior data on marine stingrays. However, this study suggests that freshwater stingrays of the family Potamotrygonidae may have a visual colour system that has ecologically adapted to a riverine habitat.

Insect attractants and plant biomass as natural food complements in pond culture of stock chub (Squalius cephalus)

The chub, Squalius cephalus (L.), is an important stocking fish in European rivers. This study investigates sustainable measures for culturing chub in earthen ponds. Two novel treatments were applied to enhance utilisation of natural pond food resources: yellow plates used as insect attractants (YIT) and plant biomass supplementation (PB) as a substrate supporting the development of natural food organisms. While no significant differences were recorded for most environmental variables, average macrozoobenthos biomass was significantly higher (p < 0.05) in the control (61.51 g.m−2) compared with PB (29.3 g.m−2) and YIT (36.8 g.m−2). However, fish from YIT ponds had significantly higher (p < 0.05) mean body weight gain (34.7 g) and SGR (1.60 g.day−1) compared to the PB (28.65 g and 1.50 g.day−1) or control (25.64g and 1.43 g.day−1) ponds. Diet analysis indicated a higher amount of animal food (mainly zooplankton, zoobenthos, terrestrial insects and fish) in YIT and PB treatments, while a higher amount of plant (mainly macrophytes) and emergency food (detritus) was observed in the control ponds. Differences in diet were reflected in the fatty acid profile of fish from different treatments, with YIT fish having significantly (p < 0.05) higher n-3 PUFA, n-3 HUFA and n-3/n-6 ratio, compared to PB fish and controls. Our results confirm that YIT use in pond aquaculture significantly enhances the role of natural food resources in the diet of cultured chub, thereby contributing to its sustainable production.

Analysis of Droplet Characteristics and Kinetic Energy Distribution for Fixed Spray Plate Sprinkler at Low Working Pressure

HighlightsAn experiment was conducted to investigate the hydraulic performance of a fixed spray plate sprinkler (FSPS).A model was developed for estimating the cumulative kinetic energy of the FSPS under a moving system.The droplet characteristics and kinetic energy distribution were affected by the working pressure and FSPS structure.A high cumulative kinetic energy could lead to a low water infiltration rate into the soil.Abstract. The kinetic energy of droplets from a fixed spray plate sprinkler (FSPS) has a substantial influence on runoff and soil erosion, as well as on the energy consumption of moving sprinkler irrigation systems. To determine the droplet characteristics and kinetic energy of an FSPS, an experiment was conducted to investigate the effects of working pressure, plate structure, and nozzle size on the droplet diameter, velocity, and kinetic energy. Two plates (the FSPSB with a concave trajectory and deep grooves in the blue plate, and the FSPSY with a flat trajectory and shallow grooves in the yellow plate) were used in the tests. The cumulative kinetic energy and water depth were calculated for a single sprinkler moving in a straight line. The results show that the FSPSB, which had deeper grooves in the plate, produced a larger droplet diameter than the FSPSY, with shallow grooves in the plate. The droplet landing velocities presented a logarithmic relationship with the droplet diameter, and velocities increased with an increase in droplet diameter. The peak specific power (SP) value of the FSPSB was 1.14 to 16.76 times that of the FSPSY. When the working pressure was less than 150 kPa, the peak SP of the FSPSB remained at a high level. With an increase in working pressure, the peak SP of the FSPSB initially increased and then decreased, while the peak SP of the FSPSY increased. The cumulative kinetic energy of the FSPSB was higher than that of the FSPSY under mobile spray conditions. Compared with the cumulative water depth, the cumulative kinetic energy of the FSPSB increased and then decreased as the working pressure increased for the same applied water volume. Because the soil had a lower infiltration rate under the FSPSB, surface ponding was more likely to occur with the FSPSB than with the FSPSY at low working pressure. Keywords: Cumulative kinetic energy, Droplet size, Specific power, Sprinkler irrigation, Working condition.

Effect of Swirl Plates on Volumetric Discharge Rate and Spray Characteristics of Hollow Cone Nozzles

The aim of the study is to determine the effect of orifice diameter, swirl plate and spray pressure on the volumetric flow, discharge coefficient and some spray characteristics of hollow cone nozzles. In the trials, five nozzle discs with 1,0, 1,2, 1,6, 2,0 and 2,4 millimetres orifice diameters and three polyacetal materials with 2 or 3 slots and one stainless steel with 2-slots swirl plate were used. Spray application were made at spray pressures of 2, 4, 6, 8 and 12 bar. The highest discharge rate at constant pressure was obtained with stainless steel and the lowest blue swirl plates. Although the number of slots was different, the effect of brown and yellow swirl plates on volumetric discharge rate variation was statistically insignificant. The discharge coefficient decreased as the diameter of the nozzle orifice increased. Accordingly, the average discharge coefficient for the 1,0 mm, 1,2, 1,6, 2,0 and 2,4 mm diameter nozzle discs was 0,411, 0,362, 0,285, 0,236 and 0.201, respectively. It was estimated that the droplet diameter in the range of 2-12 bar in the hollow cone nozzles varied between 76,3-219,0 μm and it was determined that mostly very fine and partially fine and medium sized droplets were produced.

CRYSTAL STRUCTURE AND TAUTOMERISM OF ALKYLAMINO-β-KETOENOLS IN SOLUTIONS

The structure of alkylamino-β-ketoenols obtained by opening of the pyran cycle of chalcones based on dehydracetic acid was studied by X-ray diffraction, IR, 1H NMR spectroscopy and chromatography-mass spectrometry. The infrared spectra of the compounds appear to be typical of β-ketoenols. The crystalline structure (2Z,5Z,7E,9E)-6-hydroxy-2-(methylami-no)-10-phenylethoxy-2,5,7,9-tetraene-4-one was first determined. A comparative analysis of it with 2 pre-viously published structures has been carried out. All of these compounds are crystallized in the form of yellow plates in the monoclinic crystalline system (spatial group P21/n), they have a planar arrangement of the polyene chain and β-ketoenol fragment con-jugated to the aromatic ring. It has been established that the planar configuration of the β-ketoenol fragment is stabilized by hydrogen bonds between the hydroxy and keto group and the amino and keto group in the crystalline state. The length of the polymethine chain, the nature of the substituents in the aromatic and aliphatic part of the molecule do not significantly affect the size and geometry of the alkylamino-β-ketoenolate fragment. Approximately the same lengths of bonds, the distance between atoms and the corners indicate that all these com-pounds may have similar chelating properties. Ac-cording to 1H NMR spectroscopy in various deu-terated solvents (benzene, DMSO, chloroform, ace-tone, methanol and trifluoroacetic acid), most of the main signals of the obtained compounds have sa-tellites, which indicates the existence of several isomeric forms in solutions. The analysis of integral intensities of labile protons in 1H NMR spectra does not allow to obtain accurate results regarding the ratio of tautomers in a solution, but for a series of compounds their approximate ratio in chloroform, DMSO and trifluoroacetic acid have been established (from 70:30 in CDCl3 to 90:10 in DMSO and CF3CO2D). The stability of the tautomers in the solutions is confirmed by the data of chromatogra-phy-mass spectrometry.

Survey of potential insect vectors of Rice Yellow Mottle Virus in the Southern and Central rice basin of Benin

Objectives : Insects are considered as the main vectors of Rice Yellow Mottle Virus (RYMV) in Africa. However, in Benin, little is known about the abundance and diversity of potential insect vectors of RYMV in rice fields to prevent and manage disease impact on rice production. Methodology and Results : The inventory of the potential insect vectors of this disease was carried out in the rice basin of the Southern and Central of Benin where three sites namely Africa Rice station, Koussin and Ouedeme, were prospected. The sweep net technique, visual observation and yellow plates trap were used to conduct the surveys. Eighty insect species belonging to 28 families and 8 orders were recorded at rice tillering, booting, heading and maturation stages. Variation of insect diversity was low among the sites. The shorthorned grasshopper Oxya hyla (Serville) , the ladybird beetle Chnootriba similis (Mulsant) , the rice white leafhopper Cofana spectra (Distant) and the stalk-eyed fly Diopsis thoracica (Westwood) were the most important species considering their relative abundance (10.62%, 5.19%, 7.99% and 7.01%, respectively) and frequency of occurrence (90%, 80%, 73.33% and 80%, respectively). These insects were mostly present at tillering and booting stages. Conclusion and application of results : Seventy-five (75 %) of the recorded species were not identified as RYMV vectors before. Special attention must be paid to the distribution and importance of these insects in the fields. Studies on their ability to transmit RYMV according ecological conditions should be done to know high-risk production areas and to prevent large epidemics of RYMV. Keywords : Rice-feeding insects, West Africa, virus transmission, relative abundance, occurrence, rice ecology

Synthesis of (E)-Ethyl-4-(2-(furan-2-ylmethylene)hydrazinyl)benzoate, crystal structure, and studies of its interactions with human serum albumin by spectroscopic fluorescence and molecular docking methods.

A novel hydrazone, (E)-Ethyl-4-(2-(furan-2-ylmethylene)hydrazinyl)benzoate (EFHB), has been synthesized and characterized by FT-IR, NMR and Mass spectroscopy, and X-ray diffraction; compound crystallized as translucent light yellow thin plates. EFHB was studied for their binding to human serum albumin (HSA) using the fluorescence quench titration method. Molecular docking was also performed to get a more detailed insight into their interaction with HSA at the binding site. Addition of this hydrazone to HSA produced significant fluorescence quenching and splitting of emission spectra of HSA through static quenching mechanism with binding constants of about 104 M-1 at 292.15, 298.15, 304.15 and 310.15 K. According to the synchronous fluorescence, tryptophan and tyrosine residues of the protein are most perturbed by the binding process. Thermodynamic parameters ΔG, ΔH, and ΔS were got and the main sort of acting force between EFHB and HSA was studied. Results of molecular docking have shown that EFHB binds to subdomain IIA of HSA mainly by hydrophobic interaction, energy binding are in good agreement with those obtained by fluorescence study (ΔGthe = -7.32 ± 0.09 kcal mol-1 and ΔGexp = -6.76 ± 0.03 kcal mol-1).

BIODIVERSITY AND DIET OF COCCINELLIDAE ON CITRUS IN METIDJA (ALGERIA)

Biological control is a viable alternative to the use of chemical pesticides that are harmful to the environment. From this perspective, this work aims to make an inventory of Coccinellidae and to classify them according to their diet. Sampling by yellow sticky plates is carried out each week from 23 April to 24 July 2014 at the Eastern Metidja very important citrus region in Algeria. A total of 16 ladybug species are sampled in four citrus species, orange, lemon, clementine and pomelo. It is the Coccidiphagous that are the richest with a total of 7 species, followed by the aphidophagous with 5 species and mycophagous with the two species. Finally found the aleurodiphagous and acariphagous, with a single species for each group. With regard to relative abundance, a total of 467 individuals coccinellidae were captured, of which the species Clitostethus arcuatus (ladybug aleurodiphagous) alone counted 285 individuals, that is 61.29%.