Yellow Pea Research Articles

The culinary traits: cooking time and canning quality of pulses

Pulses are one of the most important plant proteins and they contribute to food and nutrition security. Their long cooking time is a major factor that has rendered these pulses underutilized because high amount of energy is required to make them palatable. This report highlights two major culinary qualities (cooking time and canning quality) of some pulses with the aim of identifying their cooking time and canning quality methodology. It presents different reports on the cooking time and canning quality of pulses published from 2005 to 2021 for cooking time and from 1996 to 2021 for canning quality. Nineteen journals were selected for cooking time and canning quality. Google scholar served as main source of data. Key words such as determination of cooking time, and canning quality of pulses were used. This report showed that cooking methods of pulses were mainly sensory analysis, tactile, Mattson cooker, pressure cooker, VIS/NIR spectroscopy, solid loss, glass slide, and hyperspectral imaging. While canning quality parameter identified include: wash drain weight and percentage drain weight, firmness splitting, texture, visual appearance, colour, hydration coefficient, overall acceptance. The report also showed that 5% yellow pea, 5% lentil, 5% dolichos, 33% cowpea, 5% chickpea, 47% common bean were the pulses identified for cooking time. While canning quality is 6%, runner bean, 19% cowpea 75%, common bean 75%. We suggested that breeding should be focused on canning quality and shorter cooking time to enhance the utilization of these pulses. This result therefore provides information for researchers, pulse breeders, producers and agronomists in Nigeria and Africa.

Non-destructive three-dimensional characterization of micronized pulse seeds using X-ray microcomputed tomography

Non-destructive quality evaluation techniques are vital in 3D visualization and quantification of microstructural changes in micronized pulse kernels. The effects of micronization (infrared heating) on pulse microstructure are highly dependent on its treatment efficiency. This study employed X-ray micro-computed tomography (micro-CT) to characterize, visualize, and quantify the microstructural changes non-destructively. Four major pulses, chickpeas, fava beans, lentils, and yellow peas, were subjected to micronization at 180 °C of surface temperature for four different infrared (IR) exposure times (60, 80, 100, and 120 s). All samples were pre-conditioned at 20 % moisture content before the micronization process. Morphometric parameters (pore size, diameter, and pore distribution) were qualitatively measured using the reconstructed 2D and 3D images acquired by the X-ray micro-CT. Physico-chemical parameters, including loss of moisture (%), total porosity (%), volumetric expansion/shrinkage (%), and density (%) were quantified using Micro-CTAn software. The results showed that the porosity is significantly increased (p < 0.05) in all pulse types ranging from 0.60 ± 0.42 % to 12.1 ± 2.68 %, at prolonged IR exposure time. Significant volume expansion ranging from 3.82 %–33.56 % was shown by chickpeas and yellow peas, while fava beans and lentils showed volume shrinkage ranging from 4.26 %–24.31 % after micronization. Furthermore, density was decreased ranging from 0.79 ± 0.25% to 33.49 ± 0.42 % with increased IR time for all pulses except fava beans. The authors found that the microstructural changes in all tested pulses were optimum at 100 s–120 s of exposure time. Results emphasize the higher potential in modifying the microstructure of pulses using micronization processing. Therefore, future studies are suggested on developing predictive models to explore the relationships between microstructural changes and techno-functional properties.

Physico-chemical properties of pea fibre and pea protein blends and the implications for in vitro batch fermentation using human inoculum

The incorporation of fibre into pea protein matrices influences their microstructure, yet our understanding of their gut fermentability remains unexplored. In this study, dietary fibres and protein from yellow pea were investigated for their physico-chemical properties and impact on in vitro colonic fermentation using human inoculum. Pea fibre and pea protein blends were studied at different pH and after thermal treatment at 95 °C for 30 min with oscillatory rheology, static light scattering and confocal laser scanning microscopy. The effect on in vitro colonic fermentation was evaluated measuring gas production, ammonia, and short chain fatty acid (SCFA) production. Rheology indicated that during thermal treatment a firmer gel is formed close to the protein isoelectric point with a structure characterised by aggregation, but less particle swelling compared to other pH. Addition of fibre led to higher storage modulus (G′), with the fibre dominating the rheological properties. Fermentation of samples containing protein led to higher levels of ammonia and SCFA compared to only fibres. Blends produced higher amounts of valerate, i-valerate and caproate, and lower amounts of ammonia. Reduced fermentation of proteins in the presence of fibres was also reflected in a more intact microstructure of the protein particles in the digesta. Although thermal treatment of blends caused particle swelling and induced gelation, only small differences could be discerned in the in vitro colonic fermentation outcomes. Our results highlight that potentially harmful fermentation products from protein, such as ammonia, were reduced in the presence of pea hull fibre.

A comprehensive assessment of microscopic characterization techniques to accurately determine the particle size distribution of roller-milled yellow pea flours

The particle size of milled ingredients influences the quality and texture of manufactured foods. However, the limited understanding of how milling influences particle size distribution (PSD) of pulse flours, has hindered the incorporation of pulses in food formulations. This first-ot-its-kind study delves into PSD examination of roller-milled yellow pea flour streams and blends, employing laser diffraction (LD), scanning electron microscopy (SEM), and synchrotron-based X-ray microcomputed tomography (SR-μCT). LD analysis revealed a multimodal distribution pattern across particle sizes ranging from 3 to 1300 μm. SEM confirmed particle size variations among flour streams and blends. Synchrotron X-rays, known for their high intensity, brightness, and tunable energy, provided seminal insights into complex flour structures. Employing SR-μCT, the 3D volumetric particle size distribution yielded more accurate particle size and shape computations than established techniques. This pulse flour characterization will enable food manufacturers to utilize nutrient-dense pulse ingredients to address the growing market demand for health-conscious products.

In vitro gastrointestinal simulated digestion of three plant proteins: determination of digestion rate, free amino acids and peptide contents

ABSTRACT Cassava protein (CP), barley protein (BP) and yellow pea protein (YPP) are important nutrient and integral constituent of staple in pet foods. It is known that the digestion of proteins directly influences their absorption and utilisation. In the present work, we performed in vitro simulated gastrointestinal digestion of three plant proteins as a staple for dog and cat food. The digestion rate of CP, BP and YPP in dog food was 56.33 ± 0.90%, 48.53 ± 0.91%, and 66.96 ± 0.37%, respectively, whereas the digestion rate of CP, BP, and YPP in cat food was 66.25 ± 0.72%, 43.42 ± 0.83%, and 58.05 ± 0.85%, respectively. Using SDS-polyacrylamide gel electrophoresis to determine the molecular weight (MW) of each protein and the products of their digestion, it was revealed that MW of digestion samples decreased, and MW during the small intestine phase was lower than that during the gastric phase. Peptide sequences of digested products were identified by liquid chromatography-tandem mass spectrometry (LC-MS/MS), and it was found that the total number of peptides in the small intestine digestion samples was higher than that in the gastric phase samples. The MW of peptides obtained from CP was within the range of 1000–1500 Da, while MW of peptides derived from BP and YPP was within the range of 400–2000 Da. In addition, free amino acids were mainly produced in the small intestine phase. Furthermore, the percentage of essential amino acids in the small intestine phase (63 ~ 82%) was higher than that in the gastric phase (37 ~ 63%). Taken together, these findings contribute to the current understanding of the utilisation of plant proteins in dog and cat foods and provide important insights into the selection and application of plant proteins as a staple in dog and cat foods.

Volatile profile and sensory properties of gluten-free bread with yellow pea flour and sourdough

Volatile profile and sensory properties of gluten-free bread with yellow pea flour and sourdough

In vitro digestion and fermentation characteristics of eight kinds of pulses and suggestions for different populations.

Pulse-based diets are attracting attention for their potential in combating diet-related non-communicable diseases. However, limited research studies have focused on the digestive and fermentative properties of pulses, which are crucial for exerting benefits. Here, we investigated the in vitro digestibility of starch/protein, along with the fermentation characteristics, of eight pulses and their pastes, including white kidney beans, adzuki beans, cowpeas, broad beans, mung beans, chickpeas, white lentils, and yellow peas. The findings indicated that pulse flours and pastes were low GL food (estimated GL < 10) and had a low degree of protein hydrolysis during simulated gastrointestinal digestion. During in vitro fermentation, pulses flours and pastes decreased the fermentation pH, increased the level of short-chain fatty acids (mainly consisting of valeric acid, followed by acetic acid, propionic acid, butyric acid, isobutyric acid, and isovaleric acid), and positively modulated the microbiota composition over time, specifically reducing the ratio of Firmicutes to Bacteroidetes. In addition, we found that boiling could affect the in vitro digestion and fermentation characteristics of pulses, possibly depending on their intrinsic nutrient characteristics. This research could provide a comprehensive summary of the nutrient content, digestibility, and fermentation of eight pulses and their pastes. Guided by factor analysis, for different individuals' consumption, pulses, cowpeas, broad beans, white lentils, and white kidney beans were preferred for diabetic individuals, yellow peas and white lentils were preferred for intestinal homeostasis disorders, and white lentils, broad beans, white kidney beans, and cowpeas were suitable for obese individuals, in which white lentils were considered healthier and suggested for healthy adults.

Creating saponin-free yellow pea seeds by CRISPR/Cas9-enabled mutagenesis on β-amyrin synthase.

Dry pea (Pisum sativum) seeds are valuable sources of plant protein, dietary fiber, and starch, but their uses in food products are restricted to some extent due to several off-flavor compounds. Saponins are glycosylated triterpenoids and are a major source of bitter, astringent, and metallic off-flavors in pea products. β-amyrin synthase (BAS) is the entry point enzyme for saponin biosynthesis in pea and therefore is an ideal target for knock-out using CRISPR/Cas9 genome editing to produce saponin deficient pea varieties. Here, in an elite yellow pea cultivar (CDC Inca), LC/MS analysis identified embryo tissue, not seed coat, as the main location of saponin storage in pea seeds. Differential expression analysis determined that PsBAS1 was preferentially expressed in embryo tissue relative to seed coat and was selected for CRISPR/Cas9 genome editing. The efficiency of CRISPR/Cas9 genome editing of PsBAS1 was systematically optimized in pea hairy roots. From these optimization procedures, the AtU6-26 promoter was found to be superior to the CaMV35S promoter for gRNA expression, and the use of 37°C was determined to increase the efficiency of CRISPR/Cas9 genome editing. These promoter and culture conditions were then applied to stable transformations. As a result, a bi-allelic mutation (deletion and inversion mutations) was generated in the PsBAS1 coding sequence in a T1 plant, and the segregated psbas1 plants from the T2 population showed a 99.8% reduction of saponins in their seeds. Interestingly, a small but statistically significant increase (~12%) in protein content with a slight decrease (~5%) in starch content was observed in the psbas1 mutants under phytotron growth conditions. This work demonstrated that flavor-improved traits can be readily introduced in any pea cultivar of interest using CRISPR/Cas9. Further field trials and sensory tests for improved flavor are necessary to assess the practical implications of the saponin-free pea seeds in food applications.

Seeding rate and sulfur drive field pea yields in the Maritime region of Canada

The inclusion of pulse crops in Canadian rotations has the potential to improve cropping system efficiencies, reduce the overall amount of applied nitrogen, provide economic opportunities for producers, and reduce the overall carbon footprint of the cropping system. Although primarily grown in western Canada, many pulse species—field pea in particular, are well suited to temperate growing conditions in the Maritime region of Canada. A study was conducted over 2 years at Harrington, Prince Edward Island, and consisted of four field pea varieties including two yellow varieties (AAC Lacombe and CDC Saffron) and two green varieties (CDC Limerick and CDC Raezer) planted at three plant population densities: 75, 100, and 125 plants m−2. The study also measured the effects of nitrogen fertilizer applied pre-plant (0 kg ha−1 vs. 15 kg ha−1) and applied plant available sulfur (0 kg ha−1 vs. 25 kg ha−1). Overall, yellow pea varieties were higher yielding than green pea varieties, and there was a linear increase in yield with increased seeding rate. There were no significant effects of pre-plant nitrogen fertilizer on yield, although it did slightly increase seed protein. Applied sulfur had a positive effect on yield and a slightly negative effect on thousand seed weight. This experiment provides a recommendation for the optimal seeding rate (100 plants m−2) and fertility recommendations to achieve profitable yields growing field pea in the Maritime region of Canada.

Towards low-impact food products through reverse engineering: A functionality-driven approach

Currently, food products are formulated by a resource-intensive separation of agro-materials into pure components (isolates). These ingredients are subsequently combined again in food products. Alternatively, impure mildly refined ingredients require fewer resources but are difficult to use in product formulation. Machine learning can predict the properties of these impure ingredients, which facilitates their selection process. This study aims to formulate sustainable ingredient formulations based on predicted techno-functional properties instead of purity (composition). Extensively and mildly refined ingredients from yellow peas and lupine seeds are matched to a product portfolio based on gelation, viscosity, emulsifying stability, and foaming capacity. Formulations based on techno-functional properties include more milder refined ingredients and result in up to 70% reduced global warming potential, water usage, and raw materials compared to formulations based on composition and isolates only. This approach contributes to reducing the environmental impact of the food chain and should be extended to other ingredients or properties.

Functionality and nutritional properties of yellow pea, green lentil, chickpea, and navy bean proteins extracted by different methods

Functionality and nutritional properties of yellow pea, green lentil, chickpea, and navy bean proteins extracted by different methods

Development of a gluten‐free bread with added heat‐treated yellow pea flour: Application of response surface methodology

AbstractBackground and ObjectivesProcessing parameters of yellow pea (Pisum sativum L.) flour‐fortified gluten‐free (GF) bread was optimized by response surface methodology (RSM). The final bread product was evaluated for quality, sensory acceptability, and shelf life. A second‐order model was fitted to the precooking temperature of whole pea (PTWP), water level, and proof time as the factors used in RSM.FindingsHigher PTWP and lower proof time had significantly (p &lt; .05) lower bread crumb brightness compared to other treatments. Crumb firmness was influenced by the PTWP, water level, and proof time. The optimized parameters for PTWP, water level, and proof time were 156.9°C, 523.8 g, and 18.0 min, respectively. The optimized bread had a brightness (L* value), specific volume, crumb firmness, and mean cell diameter of 68.20, 2.6 mL/g, 174.2 gf, and 3.81 mm, respectively. The optimized GF bread had longer shelf‐life but had significantly (p &lt; .05) lower acceptance scores, than the commercial premix GF bread product.ConclusionThe optimized processing parameters for yellow pea utilization in gluten‐free bread were identified by RSM resulting in optimized quality characteristics of the finished baked product.Significance and NoveltyThe principles of RSM were an excellent tool to optimize the physical attributes of gluten‐free bread formulated with yellow pea flour.

Fate of pulse globulin proteins molecular Structure and composition on high moisture extrusion

Mungbean and lentil proteins are gaining attention for the production of high moisture extrusion (HME)-based meat- and dairy-analogues. However, understanding the mechanism of fibrous structure formation and molecular and higher-order changes in HME remains limited. Globulin protein isolates from mungbean (MBPI), green lentil (GLPI) and yellow pea (YPPI) along with commercial soy (CSPI) and yellow pea (CPPI) protein were investigated for their pasting properties using high-pressure rapid visco analyser (RVA), and the formation of anisotropic structures and molecular changes at constant HME conditions. Vicilin-rich MBPI showed higher viscosity on RVA, consumed higher specific mechanical energy (SME) during extrusion, and developed extrudates with higher textural strength and cutting force than proteins with both legumin and vicilin-like globulins, i.e. GLPI and YPPI. Sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) and size-exclusion chromatography (SEC) of extrudates' soluble proteins showed dissociation of vicilin-like globulins into their smaller subunits. In contrast, legumin-like globulins were reaggregated into higher molecular weight soluble macromolecules. Further, SDS-PAGE of extrudates’ total protein revealed the involvement of legumin-like globulin in the macrostructure network of the extrudates. During HME, native unfolded structures were destroyed with conversion, predominately driving changes in the tertiary structure (thermal denaturation) and secondary structure with loss of helical structure to formation of random coil and beta-sheet structures. Protein extractability in different solvents was greatly reduced, with covalent and non-covalent interactions being majorly involved in the stabilisation of extrudates structure. Protein composition, and in particular vicilin-legumin ratio and protein conformation, determined the structural development and molecular changes in HME.

Bulk and flow characteristics of pulse flours: A comparative study of yellow pea, lentil, and chickpea flours of varying particle sizes

Bulk and flow characteristics of pulse flours: A comparative study of yellow pea, lentil, and chickpea flours of varying particle sizes

A Short Communication on Functional, Rheological, and Extrusion Properties of High Protein Fractions from Pulses Obtained by Air Classification

Animal-based foods have a large impact on the environment; for this reason, plant-based meat analogous are now in the spotlight. Although protein concentrates/isolates from soy are the most used raw materials for meat analogues, some concerns about allergenicity, loss of biodiversity, and low sustainability of their production rise from their uses. In this context, high protein fractions obtained by air classification of pulse flours are here proposed as substitutes of protein concentrates. Thus, this work is aimed at understanding the aptitude of high protein fraction from pulses to be transformed into meat analogous through high moisture extrusion process. Differences in functional and rheological properties among high protein fractions resulted in different high moisture extrusion properties and thus products with different appearance. Specifically, the foaming capacity of high protein fraction from red lentil resulted in expansion at the end of the extruder creating a heterogeneous structure, whereas the high lipid content of high protein fraction from chickpea can account for the unsuitability of this raw material to high moisture extrusion. On the contrary, high protein fraction from both fava bean and yellow pea presented a fibrous structure. However, the structure and textual properties of the final products need to be further investigated.

CRISPR/Cas9-mediated lipoxygenase gene-editing in yellow pea leads to major changes in fatty acid and flavor profiles.

Although pulses are nutritious foods containing high amounts of protein, fiber and phytochemicals, their consumption and use in the food industry have been limited due to the formation of unappealing flavors/aromas described as beany, green, and grassy. Lipoxygenase (LOX) enzymes are prevalent among pulse seeds, and their activity can lead to the formation of specific volatile organic compounds (VOCs) from certain polyunsaturated fatty acids (PUFAs). As a widespread issue in legumes, including soybean, these VOCs have been linked to certain unappealing taste perception of foods containing processed pulse seeds. To address this problem in pea and as proof of principle to promote the wider use of pulses, a Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) construct was designed to create null alleles (knockouts) of PsLOX2 which had been implicated in the generation of VOCs in peas. Successful CRISPR/Cas9-mediated LOX gene editing of stable transgenic pea lines (TGP) was confirmed by DNA sequencing of the wild type (WT) and TGP pslox2 mutant lines. These lines were also assessed for LOX activity, PUFA levels, and VOCs. Compared to WT peas, the TGP lines showed a significant reduction (p < 0.05) in LOX activity and in the concentration of key VOCs, including hexanal, 2-hexenal, heptanal, (E)-2-heptenal, (E,E)-2,4-heptadienal, 1-octen-3-ol, octanal, (E)-2-octenal (E,E)-2,4-nonadienal and furan-2-pentyl. The content of two essential PUFAs, linoleic and α-linolenic acids, the known substrates of LOX in plants, was higher in TGP flours, indicating the efficacy of the CRISPR-mediated gene editing in minimizing their oxidation and the further modification of PUFAs and their products. The collection of VOCs from the headspace of ground pea seeds, using a portable eNose also distinguished the TGP and WT lines. Multiple regression analysis showed that LOX activity correlated with the two VOCs, heptanal and (E,E)-2,4-heptadienal in pea flours. Partial Least Squares Regression (PLS-R) plot for selected PUFAs, VOCs, and sensor responses in WT and TGP lines showed distinct clusters for WT and TGP lines. Together this data demonstrates the utility of CRISPR mediated mutagenesis of PsLOX2 to quickly improve aroma and fatty acid (FA) profiles of pea seeds of an elite Canadian variety.

Effect of heating of pea fibres on their swelling, rheological properties and in vitro colon fermentation

Dietary fibre intake is essential for all human beings and has been correlated to beneficial health effects. Pea hull fibres (PF) are generally seen as a side stream during extraction of protein and starch from yellow pea but could be used in various food products to boost fibre content. In this study, the thermal treatment of pea hull fibres was investigated in terms of physicochemical properties and in vitro colonic fermentation. The PF that was subjected to heating showed an increase of fibres solubilised in the liquid and particle size. Results also showed that viscosity and storage modulus increased with thermal treatment, possibly due to the swelling of the PF. The pea fibre was readily fermentable based on total gas production and pH. However, the susceptibility to fermentation of PF did not increase with thermal treatment. Total gas production and short chain fatty acid produced were similar independent of thermal treatment. Conclusively, heating of the PF resulted in increased ability to structure water suspension, owing to increased fibre particle size, but is not sufficient to increase short chain fatty acid production during colonic fermentation. To explain this, we propose that the changes in cell wall structure were not major enough to induce higher fermentability.

Toxic Analysis of Leaf Protein Concentrate Regarding Common Agricultural Residues

&#x0D; &#x0D; &#x0D; &#x0D; Background: Potential resilient foods which help reduce hunger are converting the ~998 million tons of agricultural residue generated each year into human edible food. Although it is possible to extract Leaf Protein Concentrate (LPC) from agricultural residues, it is not widely practiced because both toxicity and yields of the protein concentrates have not been widely investigated in the most common agricultural residues.Methods: To fill this knowledge gap, this study uses high-resolution mass spectrometry and an open-source toolchain for non-targeted screening of toxins of nine agricultural plant residues in October 2021; it included seven agricultural residues: corn/maize, wheat, barley, alfalfa, yellow pea, sunflower, canola/rapeseed, and two weeds/agricultural residues of kochia, and round leaf mallow.Results: The average yield ranged from about 7 to 14.5% for the nine LPCs investigated. According to the results, yellow pea, round leaf mallow, and canola are recommended for further investigation and scaling as they appear to be fit for human consumption based on the lack of dangerous toxins found in the analysis performed in this study.Conclusion: All the compounds identified in these samples have either been approved by international regulatory boards for safe consumption or are known to be present in common beverages. The other agricultural residues require additional quantification of the toxins identified as it will determine the actual risk for human consumption. Overall, the potential for LPC to provide more needed calories from existing agricultural practices is extremely promising, but substantial amount of future work is needed to screen LPCs in all the agricultural residues depending on harvesting, handling, and storage conditions.&#x0D; &#x0D; &#x0D; &#x0D;

Understanding Protein Functionality and Its Impact on Quality of Plant-Based Meat Analogues.

A greater understanding of protein functionality and its impact on processing and end-product quality is critical for the success of the fast-growing market for plant-based meat products. In this research, simple criteria were developed for categorizing plant proteins derived from soy, yellow pea, and wheat as cold swelling (CS) or heat swelling (HS) through various raw-material tests, including the water absorption index (WAI), least gelation concentration (LGC), rapid visco analysis (RVA), and % protein solubility. These proteins were blended together in different cold-swelling: heat-swelling ratios (0:100 to 90:10 or 0-90% CS) and extruded to obtain texturized vegetable proteins (TVPs). In general, the WAI (2.51-5.61 g/g) and protein solubility (20-46%) showed an increasing trend, while the LGC decreased from 17-18% to 14-15% with an increase in the % CS in raw protein blends. Blends with high CS (60-90%) showed a clear RVA cold viscosity peak, while low-CS (0-40%) blends exhibited minimal swelling. The extrusion-specific mechanical energy for low-CS blends (average 930 kJ/kg) and high-CS blends (average 949 kJ/kg) was similar, even though both were processed with similar in-barrel moisture, but the former had substantially lower protein content (69.7 versus 76.6%). Extrusion led to the aggregation of proteins in all treatments, as seen from the SDS-PAGE and SEC-HPLC analyses, but the protein solubility decreased the most for the high-CS (60-90%) blends as compared to the low-CS (0-40%) blends. This indicated a higher degree of crosslinking due to extrusion for high CS, which, in turn, resulted in a lower extruded TVP bulk density and higher water-holding capacity (average 187 g/L and 4.2 g/g, respectively) as compared to the low-CS treatments (average 226 g/L and 2.9 g/g, respectively). These trends matched with the densely layered microstructure of TVP with low CS and an increase in pores and a spongier structure for high CS, as observed using optical microscopy. The microstructure, bulk density, and WHC observations corresponded well with texture-profile-analysis (TPA) hardness of TVP patties, which decreased from 6949 to 3649 g with an increase in CS from 0 to 90%. The consumer test overall-liking scores (9-point hedonic scale) for TVP patties were significantly lower (3.8-5.1) as compared to beef hamburgers (7.6) (p < 0.05). The data indicated that an improvement in both the texture and flavor of the former might result in a better sensory profile and greater acceptance.

Exploring the microbial diversity of novel misos with metagenomics

Interest in fermented foods, especially plant-based ones, has increased considerably in the last decade. Miso—a Japanese paste traditionally fermented with soybeans, salt, and kōji (Aspergillus oryzae grown on grains or beans)—has gained attention among chefs for its rich flavour and versatility. Some chefs have even been experimenting with making novel misos with untraditional substrates to create new flavours. Such novel fermented foods also offer new scientific opportunities. To explore the microbial diversity of these new traditional foods, we sampled six misos made by the team at a leading restaurant called Noma in Copenhagen (Denmark), using yellow peas (including a nixtamalised treatment), lupin seeds, Swedish Vreta peas, grey peas, and Gotland lentils as substrates. All misos were made with the same recipe and fermented for 3 months at 28 °C. Samples were collected at the end of fermentation for subsequent shotgun metagenomic sequencing and a genome-resolved metagenomic analysis. The taxonomic profile of the samples revealed the presence of kōji mould (A. oryzae) and Bacillus amyloliquefaciens in all misos. Various species of the genera Latilactobacillus, Lactiplantibacillus, Pediococcus and Staphylococcus were also detected. The Metagenome-Assembled Genomes (MAGs) revealed genomic sequences belonging to 12 different species and functional analyses of these MAGs were performed. Notably, we detected the presence of Exiguobacterium—the first reported instance of the genus in miso—and Average Nucleotide Identity (ANI) analyses suggest a potentially new species. We hope these results will improve the scientific literature on misos and contribute to developing novel fermented plant-based foods.