Yellow Fluorescence Research Articles

Fluorescein sodium in the surgical treatment of pleomorphic xanthoastrocytomas: Results from a retrospective study.

Pleomorphic xanthoastrocytoma (PXA) is a rare brain tumor, most commonly affecting children and young adults. Surgical resection represents the mainstay of treatment, and extent of resection is associated with improved survival. In this study, we analyzed the role of sodium fluorescein (SF) in improving intraoperative visualization easing resection. Surgical database of FLUOCERTUM study (Besta Institute, Milan, Italy) was retrospectively reviewed to find pleomorphic xanthoastrocytomas and anaplastic xanthoastrocytomas, according to WHO-2016/2021 classification, surgically removed by a fluorescein-guided technique from March 2016 to February 2022. SF was intravenously injected (5mg/kg) immediately after induction of general anesthesia. Tumors were removed using a microsurgical technique with the YELLOW 560 filter (Carl Zeiss Meditec, Oberkochen, Germany). Twelve patients (7 males and 5 females; 3 pediatric patients, mean age 10 years, range 5 to 13 years and 9 adult patients, mean age 50.6 years, range 35 to 63 years) underwent fluorescein-guided surgery. No side effects related to SF occurred. In all tumors, contrast enhancement on preoperative MRI correlated with intense, heterogeneous yellow fluorescence with bright fluorescent cystic fluid. Fluorescein was considered helpful in distinguishing tumors from viable tissue in all cases. Gross total resection was achieved in 8 cases (66.7%); in 4 cases, otherwise, the resection was subtotal with fluorescent residual spots to avoid neurological worsening (33.3%). The use of SF is a valuable method for safe fluorescence-guided tumor resection. Our data documented a positive effect of fluorescein-guided surgery on intraoperative visualization, suggesting a probable role in improving the extent of resection during yellow surgery of PXA.

Methyl orange-derived carbon dots for light-emitting-diode and fluorescent electrospun nanofibers

Carbon dots (CDs) have gained tremendous attention over a wide range from fluorescence sensors to optoelectronic devices. In this context, N, S-codoped carbon dots (MO-CDs) of 2.7 nm were synthesized via a facile hydrothermal method using a single precursor Methyl Orange (MO). Upon excitation at 430 nm, the MO-CDs exhibit a yellow emission with an absolute quantum yield (QY) of 15.3%. In addition, the as-prepared MO-CDs possess excellent resistance to high ion strength, high H2O2 concentration, and UV exposure. Because of their good fluorescence properties, a light-emitting diode (LED) device that emits yellow light was fabricated by mixing MO-CDs and siloxane. Moreover, the electrospun nanofibers with yellow fluorescence were prepared by electrospinning technology. This work shows that the CDs derived from MO possess special fluorescent characteristics, which can be utilized as a multifunctional carbon material.

Fabrication of hydrophilic luminescent zinc oxide quantum dots for selective detection of copper ions and efficient inhibition of harmful fungi

In this study, we have successfully prepared surface modified zinc oxide quantum dots (M-ZnO QDs) with ultra-stable fluorescence and excellent hydrophilicity through introducing (3-aminopropyl)triethoxysilane (APTES). The as-prepared M-ZnO QDs under the optimum condition presented strong yellow fluorescence emission under 355 nm excitation and showed satisfied reproducibility. Physical and chemical properties of the synthesized ZnO QDs were further studied by various characterization techniques. Transmission electron microscopy showed homogeneous distribution of spherical M-ZnO QDs with the average particle size of 4.03 nm. According to the characteristic that metal ions can quench fluorescence, M-ZnO QDs-based fluorescence sensor for the detection of Cu 2+ in aqueous solution is developed in this work, which has the advantages of excellent selectivity, good sensitivity and a wide linear range. The limit of detection was 0.51 μM and the linear detection range was 1–200 μM for Cu 2+ determination. The practicability of the fluorescent probe is further validated in the lake water and the satisfactory spiked recoveries of Cu 2+ ranges from 99.1 % to 108.8 %. Besides, M-ZnO QDs displayed concentration inhibition effect and strain effect on the growth of fungi. Thus, the as-prepared M-ZnO QDs are demonstrated to be promising for Cu 2+ determination and anti-fungal applications.

Surgery of optic-chiasmatic tumors assisted by sodium fluorescein yellow fluorescence and visual evoked potentials monitoring.

Tumors of the anterior visual pathways are challenging to operate because of the crucial balance between extent of resection and sight preservation. Sodium fluoresceine (SF) has already been reported in maximizing brain tumor resection but not when the optic-chiasmatic region is involved. The role of Visual Evoked Potentials (VEPs) in optic-chiasmatic tumors is still debated. The simultaneous use of both technique in this setting has not been reported so far. The aim of our work is to analyse the intraoperative combination of the use of SF and VEPs in the anterior visual pathway tumor surgery. Clinical and intraoperative data from six surgical procedures on four patients affected by optic-chiasmatic tumors with preoperative visual deficits were retrospectively analyzed. In five procedures VEPs remained intraoperatively stable and no postoperative worsening of visual function was reported. In 1 case an intraoperative VEPs deterioration was predictive of a postoperative visual worsening. In all cases, tumor was fluorescent, and SF was intraoperatively helpful in guiding resection. The pattern of fluorescence was different according to the lesion histology. Based on our preliminary experience, the simultaneous combination of SF and VEPs in surgery of the optic pathway tumors seems helpful to maximize tumor resection and predict postoperative functional outcome. Since we presented a small series, larger studies are needed to confirm our data.

Multiregulated color and fluorescence of a cyanostilbene-based AIEgen by light and pH

Stimuli-responsive aggregation-induced emission (AIE) materials with regulated color and fluorescence have shown promising applications in smart optical systems, while most of them are only regulated by a single stimulus. Herein, a cyanostilbene-based fluorophore (Z-BDPA) with AIE enhancement properties is elaborately designed, which exhibits multiple regulable color and fluorescence owing to its various photochemical reactions and reversible pH response. After exposure to 420 nm light, the green emission of Z-BDPA in tetrahydrofuran (THF) becomes brighter owing to the Z→E isomerization, which can be recovered under 365 nm irradiation; the yellow fluorescence of Z-BDPA in THF/H2O mixtures with 90% water fraction (fw) becomes stronger yellow-green fluorescence due to the photodimerization. At acidic conditions, Z-BDPA powder with yellow fluorescence can become another AIEgen Z-p-BDPA with blue fluorescence via protonation, which would be recovered through deprotonation under basic conditions. Under ultraviolet light irradiation, the blue fluorescence of Z-p-BDPA is blue-shifted and increased due to the photo-isomerization and subsequent photocyclodehydrogenation reactions. Based on the regulable color and fluorescence upon the photochemical and pH responses, Z-BDPA has been successfully applied in photo-patterning and anti-counterfeiting.

Intramolecular C-H⋅⋅⋅O Hydrogen-Bonded Solid Emitter as Colorimetric and Fluorometric Cyanide-Selective Chemodosimeter.

An indole-indanedione-based small molecule, compound 1Me, comprising an intramolecular C-H⋅⋅⋅O hydrogen-bond (H-bond) and with an intramolecular charge transfer (ICT) characteristic was prepared for the colorimetric and fluorometric detection of CN- . Compound 1Me is virtually nonfluorescent in solution but emits a strong yellow fluorescence in the solid state because of the intramolecular C-H⋅⋅⋅O H-bond, which inhibits the free rotation of the exocyclic C-C single bond of 1Me. The nucleophilic addition of CN- to the β-conjugated carbon of the indanedione group of 1Me, which breaks the intramolecular C-H⋅⋅⋅O H-bond and blocks ICT, causes the colorimetric and fluorometric responses of 1Me to CN- . Furthermore, inexpensive and portable paper-based test kits containing 1Me could be used to rapidly and conveniently sense CN- using the naked eye in real time. The results provide a new strategy for designing colorimetric and fluorometric cyanide-selective sensors based on an intramolecular C-H⋅⋅⋅O hydrogen bonded solid emitter.

A Broadband, Multiplexed-Visible-Light-Transport in Composite Flexible-Organic-Crystal Waveguide.

We report the construction of an organic crystal multiplexer using three chemically and optically different acicular, flexible organic crystals for a broadband, visible light signal transportation. The mechanical integration of a highly flexible crystal waveguide of (Z)-2-(3,5-bis(trifluoromethyl)phenyl)-3-(7-methoxybenzo[c][1,2,5]thiadiazol-4-yl)acrylonitrile (BTD2CF3 ) displaying bright yellow (λ1 ) fluorescence with blue-emitting (λ2 ) BPP and cyan emitting (λ3 ) DBA crystals using AFM-tip provides a composite organic crystal multiplexer. The constructed hybrid single crystal multiplexer effectively transduces three optical signals (λ1 +λ2 +λ3 ) covering the 420-750 nm region as a composite output signal. The presented proof-of-principle experiment demonstrates the real potential of organic flexible crystal waveguides for visible light communication technologies.

A Broadband, Multiplexed‐Visible‐Light‐Transport in Composite Flexible‐Organic‐Crystal Waveguide

AbstractWe report the construction of an organic crystal multiplexer using three chemically and optically different acicular, flexible organic crystals for a broadband, visible light signal transportation. The mechanical integration of a highly flexible crystal waveguide of (Z)‐2‐(3,5‐bis(trifluoromethyl)phenyl)‐3‐(7‐methoxybenzo[c][1,2,5]thiadiazol‐4‐yl)acrylonitrile (BTD2CF3) displaying bright yellow (λ1) fluorescence with blue‐emitting (λ2) BPP and cyan emitting (λ3) DBA crystals using AFM‐tip provides a composite organic crystal multiplexer. The constructed hybrid single crystal multiplexer effectively transduces three optical signals (λ1+λ2+λ3) covering the 420–750 nm region as a composite output signal. The presented proof‐of‐principle experiment demonstrates the real potential of organic flexible crystal waveguides for visible light communication technologies.

Dual Detection of Temperature And Chiral Amino Acid Using Triphenylamine‐Based Fluorescent Probes

AbstractAccuracy measurement of temperature is important in day‐to‐day life and scientific research. Chiral amino acids play an irreplaceable role in medicine, biochemistry, and other fields; however, the polarity solvents do easily disturb hydrogen bond formation between chiral amino acids and fluorescence probe. Herein, a fluorescent probe Tap‐S, using triphenylamine as fluorophore, is synthesized for dual detection of temperature and enantiomers of chiral amino acids in polarity aqueous solution. The fluorescence emission of Tap‐S to the temperature has a good response in the mixed solvent of methanol and glycerin over a wide temperature range (−45 to 50 °C). The gradient thermosensitivity, accuracy and reversibility of Tap‐S to the temperature in our strategy are achieved. Tap‐S can emit yellow fluorescence in the high‐viscosity mixed solution. By taking advantage of the viscosity measurement, the restricted intramolecular rotation mechanism of Tap‐S's fluorescence emission was effectively clarified. Furthermore, the potential use of Tap‐S to selective detect L‐phenylalanine of chiral amino acids has been successfully executed in the polarity aqueous solution at the room temperature (25 °C). The complex of Tap‐S and L‐phenylalanine can inhibit the rotation of the single bond of Tap‐S inducing the fluorescence emission. This work provides a novel strategy for the studies on not only the detection of temperature but also the enantiomers of chiral amino acids.

Synthesis of a Single Benzene-Based Fluorophore for Selective Recognition of Al Ions

Though 2,5-dihydroxyterephthalic acid (DHT) is composed of a single benzene molecule, it is blue-emissive in common organic solvents and in the solid state. Like most organic fluorophores, DHT is not soluble in water, which limits its versatile use in metal ion detection in an aqueous medium. To improve the water solubility of DHT and its use as a molecular sensor in aqueous solutions, its deprotonated form, DHT-K, was synthesized through the simple one-pot reaction of DHT with KOH. Compared with DHT, DHT-K was highly soluble in water and emitted yellow fluorescence in the solution and the solid. In addition, DHT-K showed high selectivity for Al ions, exhibiting fluorescence wavelength changes from 540 to 495 nm depending on the Al ion concentration. A linear relationship between the fluorescence intensity of DHT-K and Al ion concentration was established ranging from 18.96 to 247 μM with a detection limit of 1.84 µM. The binding stoichiometry between DHT-K and Al ions was determined by Job’s plot and found to be 1:2. Upon exposure to Al ions, DHT-K showed significant changes in fluorescence color and emission wavelength, whereas no fluorescence changes were observed by the addition of various metal ions such as Zn2+, Mg2+, Ca2+, Cu2+, Fe3+, and Co2+. Thus, DHT-K can be applied as a fluorescent sensor that can selectively detect Al ions in aqueous solutions.

Modification of erythrocytes by internalizing Arg-Gly-Asp (iRGD) in boosting the curative effect of radiotherapy for gastric carcinoma.

Radiation resistance remains the leading cause of radiotherapy (RT) failure. The development of tumor-specific targeted sensitizers is key to overcoming radiation resistance. Our early data showed that cancer cell penetration was simulated by internalizing arginine-glycine-aspartic acid (iRGD), and the irradiation efficacy was improved. The present study aims to design and fabricate iRGD-modified red blood cell (RBCs) for tumor targeting and RT enhancement, and to evaluate its safety and efficacy in vivo. 1,2-Distearoyl-sn-glycero-3-phosphoethanolamine-poly ethylene glycol-iRGD (DSPE-PEG-iRGD) was used to modify RBCs by a lipid-insertion method without direct chemical bioconjugation. Fluorescent dyes were used to trace the functional RBCs through confocal microscopy examination. In vitro stability evaluation was performed using cell culture medium incubation for 48 h followed by fluorescence decay assay. Furthermore, a subcutaneous cancer cell mouse model was constructed with MKN-45 cells for target efficacy and RT enhancement evaluation with DSPE-PEG-iRGD-modified RBCs (RBC-iRGD). Successful construction of RBC-iRGD was verified by the presence of the yellow fluorescence, and an approximately 108 iRGD molecules were labeled on a single RBC. The final RBC-iRGD showed good stability without any hemolytic effects in the cell culture medium. Moreover, higher fluorescence intensity and decreased liver and spleen accumulation could be observed in RBC-iRGD compared to RBC + iRGD in vivo. The RBC-iRGD exerted enhanced radiosensitivity in subcutaneous gastric tumor mice. The RBC-iRGD exerted good tumor-targeting efficacy and favorable effects for RT enhancement in vivo.

Carbon dot-decorated metal-organic framework composite for Fe3+ detection in the aqueous environment

The contamination of heavy metal ions in the aqueous environment constitutes an important threat to human survival. Hence, the development of simple, effective, and cheap detection of heavy metals and pesticide residues is essential for environmental control. Here, we synthesized a yellow carbon dots-linked ZIF-8 (yCDs/ZIF-8) composite, which was prepared with excellent photoluminescence properties and aqueous stability, and characterized by strong yellow fluorescence emission. The ratio metric fluorescence sensor has been constructed using its stable light blue carbon dots. The fluorescence sensor has excellent selectivity and sensitivity as a sensing probe for Fe3+ ions, with a detection limit of 0.3690 μM. In addition, the fluorescence quench mechanism was investigated. It has been shown that the yCDs/ZIF-8 fluorescence could be quenched by Fe3+ via static quenching and absorption competition mechanisms. The probe has high-quality application potential in terms of food safety and environmental monitoring due to the fact of its simplicity, high yield, and low cost.

Highly sensitive and selective detection of tryptophan by antipyrine based fluorimetric sensor

For the detection of tryptophan (TP), organic molecule based on antipyrine (AP) has been synthesized. UV-vis and fluorimetric study has been used to confirm the sensing mechanism of AP with TP and theoretical explanations based on DFT calculations were also employed. The presence of numerous interfering biomolecules such alanine, asparagine, ascorbic acid, creatine, creatinine, cysteine, dopamine, glutamine, histidine, leucine, phenylalanine proline and urea could be optically distinguished by the AP by a change in colour from yellow to intense yellow fluorescence. The impressive sensing performance of AP sensor exhibits rapid response, remarkable sensitivity and selectivity towards TP with excellent limit of detection, high quantum yield and binding constant of 2 × 10−7 mol/L, 0.89 and 1.12 × 106 M−1 respectively.

A ratiometric fluorescence method based on nitrogen-doped carbon quantum dots for the determination of the activity of alkaline phosphatase.

Herein, a simple and sensitive ratiometric fluorescence sensing platform to detect alkaline phosphatase (ALP) activity is developed on the basis of yellow fluorescent nitrogen-doped carbon quantum dots (YNCDs). The hydrolysis of ascorbic acid 2-phosphate (AAP) into ascorbic acid (AA) is catalyzed by ALP. Then, AA will react with o-phenylenediamine (OPD) to form 3-(1,2-dihydroxyethyl)furo[3,4b]-quinoxaline (QXD) which is a blue fluorescent quinoxaline derivative with emission at 435nm in the presence of Cu2+. YNCDs have yellow fluorescence emission at 555nm, and can maintain stable in QXD reaction system. Therefore, by utilizing the fluorescence of YNCDs at 555nm as reference signal and the fluorescence of QXD at 435nm as report signal, we can detect the ALP activity by monitoring the fluorescence ratio (F435/F555). The linear range is 0.5-5 U/L, and the limit of detection is 0.14 U/L. An application of this method for the analysis of ALP in human serum has given satisfactory results. A ratiometric fluorescent nanoprobe for ascorbic acid and alkaline phosphatase detection with excellent biocompatible and high sensitivity was successfully constructed based on YNCDs and QXD.

In situ fluorogenic reaction for ratiometric fluorescent detection of alkaline phosphatase activity

Assays utilizing in situ fluorogenic reactions provide a simple and convenient alternative approach for the detection of biological molecules and activities. In this work, a novel ratiometric fluorescent probe based on in situ fluorogenic reaction is explored and developed for alkaline phosphatase (ALP) activity sensing. An intriguing fluorogenic reaction between 2,3-diaminonaphthalene (2,3-DAN) and ascorbic acid (AA) in alkaline aqueous solutions could generate the fluorescent quinoxaline derivative. The resultant quinoxaline emits intense yellow fluorescence, differing from the blue fluorescence of 2,3-DAN. Thus, a ratiometric fluorescent probe based on this fluorogenic reaction is constructed for ALP activity sensing, combining with ALP-triggered hydrolysis of AA2P into AA. Meanwhile, the addition of copper(II) acetate into the reaction system largely improves reaction rate and efficiency. This sensing strategy shows high sensitivity for ALP activity with detection limit of 0.08 U/L, and excellent selectivity towards ALP out of various interferences. This method is extended to human salivary ALP detection. The present method provides a simple and reliable alternative for the detection of ALP activity and has the potential for clinical applications. It also shows a feasible way to construct ratiometric fluorescent methods.

Visible–light–modulating cucurbit[8]uril–mediated reversibly tunable Multicolor–Light emission

Multicolor emission materials in response to visible light stimuli have good prospects for application in biomedical engineering and materials science. Herein, a dual–visible–light responsive intelligent quaternary supramolecular assembly 3 with tunable multicolor fluorescence outputs is constructed from dithienylethene (DTE) derivative 1, terpyridine imidazole derivative 2, Eu(NO3)3 and cucurbit (Qasim et al., 2017) [8]uril (CB (Qasim et al., 2017) [8]. Our experimental results indicate that both the opened and the closed forms of 1 exhibit blue fluorescence emission at 447 nm, and then complexes with CB (Qasim et al., 2017) [8] to form binary assembly 1@CB (Qasim et al., 2017) [8] with yellow fluorescence ON/OFF switching property at 540 nm by varying the wavelength of the exposed light. Compared with 1@CB (Qasim et al., 2017) [8], the quaternary supramolecular assembly 3 shows three different photoluminescent colors from yellow, white to blue by varying the irradiation time. Moreover, the solid−state assembly exhibited effective photoinduced FRET phenomenon. To our delight, the quaternary assembly was successfully used as photocontrolled cell−imaging agent and as a photoerasable fluorescent ink.

Aggregation-induced emission and distinct mechanochromic luminescence based on symmetrical D-A-D type and unsymmetrical D-A type carbazole functionalized dicyanovinyl derivatives

The π-conjugated luminophores CZ-DPMN and DCZ-DPMN, which are composed of electron-rich carbazole group and electron-deficient dicyanovinyl unit, are designed and synthesized. Spectral studies and theoretical calculations affirm that both compounds show unique ICT (intramolecular charge transfer) process and donor-acceptor (D-A) type molecular structure with highly distorted conformation. CZ-DPMN and DCZ-DPMN also exhibit AIE (aggregation-induced emission) characteristics at high water fraction in THF/water mixtures. Importantly, CZ-DPMN and DCZ-DPMN display remarkable fluorescent response to mechanical force. The as-synthesized solids of CZ-DPMN and DCZ-DPMN emit bright green and yellow fluorescence, and the emitting-colors change into yellow and orange-yellow after grinding, respectively. The spectral shifts are 62 nm and 22 nm upon the treatment of grinding. This MCL (mechanochromic luminescence) phenomenon is reversible during repeating grinding-fuming treatment. PXRD diffraction patterns indicate that the change of molecular internal structure from crystalline state to amorphous state after the treatment of grinding is the direct cause of MCL phenomenon.

Dual-Channel Recognition of Human Serum Albumin and Glutathione by Fluorescent Probes with Site-Dependent Responsive Features.

Design of chemical probes with high specificity and responses are particularly intriguing. In this work, a fluorescent probe (M-OH-SO3) with dual-channel spectral responses toward human serum albumin (HSA) is presented. By employing dinitrobenzenesulfonate as a recognition site as well as a fluorescence quencher, probe M-OH-SO3 displayed weak fluorescence, which, nevertheless, exhibits extensive yellow (575 nm) and red (660 nm) fluorescence emissions toward HSA under excitations at 400 and 500 nm, respectively. Interestingly, M-OH-SO3 displayed the best performance toward HSA with distinctly higher selectivity than that of its counterparts M-SO3, M-H-SO3, and M-F-SO3, which were prepared simply by modulating the functional group at the ortho position of the dicyanoisophorone core. Molecular docking results revealed that M-OH-SO3 possesses the lowest binding energy among the tested derivatives and accordingly the strongest binding affinity. Probe M-OH-SO3 showed a good linear relationship toward HSA in a range of 0.5-18 μM with a limit of detection of 35 nM. Cell imaging results demonstrated that probe M-OH-SO3 could visualize the variation HSA levels in hepatocarcinoma cells. In addition, probe M-OH-SO3 could also be employed for the recognition of glutathione through the cleavage of the dinitrobenzenesulfonate group along with an enhancement of emission at 575 nm. The site-dependent properties inspired a novel paradigm for design of fluorescent probes with optimized selectivity and responses.

Low-temperature sintered Pr3+/Er3+ codoped Bi2Mo2O9 microcrystals for multimode optical thermometry and anti-counterfeiting

Lanthanide-doped oxide materials generally require high sintering temperatures for optical applications such as temperature sensing and anti-counterfeiting. In this work, the highly crystalline 1%Pr3+/1%Er3+ co-doped Bi2Mo2O9 phosphor is reported with a low sintering temperature of 923 K. Pr3+ in the Bi2Mo2O9 host has a narrow emission band of 3P0–3H6 transition at 624 nm due to the suppressed broadening of electronic transitions in the homogeneous crystal field. Multimode temperature sensing is realized based on the upconversion and downshifting emissions as well as the luminescence lifetimes of the as-prepared phosphor. The temperature sensing based on the downshifting emission bands of this phosphor at 530 and 624 nm achieves the maximum absolute and relative sensitivities of 0.693 K−1 at 568 K and 1.88% K−1 at 483 K, respectively. For anti-counterfeiting applications, this phosphor can display bright yellow fluorescence at the liquid nitrogen cooling temperature, rather than white or pale colors. The white-color emission of this phosphor excited by 254 nm can also be used for lighting. The lanthanide-doped Bi2Mo2O9 can achieve favorable luminescence performance at much lower sintering temperature than most oxide phosphors, and is not only stable but also has the potential for in-situ sintering on materials that are not resistant to high temperature.

A sequential dual-key-dual-lock fluorescent probe for detection of SO2 and H2O2 in cells and mice

Oxidative stress refers to the disruption of the oxidative balance in the body, which can lead to various pathological conditions. There have been more and more studies on the interaction between H2O2 and reducing agents. Herein, a novel fluorescent probe BN-DUAL was designed and synthesized for sequential detecting SO2 and H2O2 with the “dual-key-dual-lock” strategy. To the best of our knowledge, this work is the first application of “dual-key-dual-lock” strategy to identify SO2 and H2O2. BN-DUAL interacted with SO2 to generate an intermediate product BN-SO2, and the pink fluorescence of the probe quenched. BN-SO2 reacted with H2O2 to generate BN with noticeable yellow fluorescence. In addition, the probe does not react directly with hydrogen peroxide. The “dual-key-dual-lock” strategy can improve the selectivity and accuracy of the detection of SO2 and H2O2 in cells and in vivo. The signal changes of the two channels can make the detection clearer and more visualized, which has been verified by imaging exogenous and endogenous SO2/H2O2 in cells and mice. This probe provides a better method to research the oxidative stress caused by SO2 and H2O2.