Yellow Fever Mosquito Aedes Aegypti Research Articles

From macro to micro: De novo genomes of Aedes mosquitoes enable comparative genomics among close and distant relatives.

The yellow fever mosquito (Aedes aegypti) is an organism of high medical importance because it is the primary vector for diseases such as yellow fever, Zika, dengue, and chikungunya. Its medical importance has made it a subject of numerous efforts to understand their biology. One such effort, was the development of a high-quality reference genome (AaegL5). However, this reference genome was sourced from a highly inbred laboratory strain with unknown geographic origin. Thus, the reference is not representative of a wild mosquito, let alone one from its native range in sub-Saharan Africa. To better understand the genetic architecture of Ae. aegypti and their sister species, we developed two de novo chromosome-scale genomes with sequences sourced from single individuals: one of Ae. aegypti formosus (Aaf) from Burkina Faso and one of Ae. mascarensis (Am) from Mauritius. Both genomes exhibit high contiguity and gene completeness, comparable to AaegL5. While Aaf exhibits high degree of synteny to AaegL5, it also exhibits several large inversions. We further conducted comparative genomic analyses using our genomes and other publicly available culicid reference genomes to find extensive chromosomal rearrangements between major lineages. Overrepresentation analysis of expanded genes in Aaf, AaegL5, and Am revealed that while the overarching category of genes that have expanded are similar, the specific genes that have expanded differ. Our findings elucidate novel insights into chromosome evolution at both microevolutionary and macroevolutionary scales. The genomic resources we present are additions to the arsenal of biologists in understanding mosquito biology and genome evolution.

Evidence for Significant Skew and Low Heritability of Competitive Male Mating Success in the Yellow Fever Mosquito Aedes aegypti.

Aedes aegpyti mosquitoes are vectors of several viruses of major public health importance, and many new control strategies target mating behaviour. Mating in this species occurs in swarms characterised by male scramble competition and female choice. These mating swarms have a male-biased operational sex ratio, which is expected to generate intense competition among males for mating opportunities. However, it is not known what proportion of swarming males successfully mate with females, how many females each male is able to mate with, and to what extent any variation in the male mating success phenotype can be explained by genetic variation. Here, we describe a novel assay to quantify individual male mating success in the presence of operational sex ratios characteristic of Ae. aegypti. Our results demonstrate that male mating success is skewed. Most males do not mate despite multiple opportunities, and very few males mate with multiple females. We compared measures of male mating success between fathers and sons and between full siblings to estimate the heritability of the trait in the narrow and broad sense, respectively. We found significant broad sense heritability estimates but little evidence for additive genetic effects, suggesting a role for dominance or epistatic effects and/or larval rearing environment in male mating success. These findings enhance our understanding of sexual selection in this species and have important implications for mass-release programmes that rely on the release of competitive males.

Larvicidal Activity of Hemp Extracts and Cannabidiol against the Yellow Fever Mosquito Aedes aegypti.

To mitigate pyrethroid resistance in mosquito vectors of emerging and re-emerging human pathogens, there is an urgent need to discover insecticides with novel modes of action. Natural alternatives, such as extracts derived from plants, may serve as substitutes for traditional synthetic insecticides if they prove to be sustainable, cost-effective, and safe for non-target organisms. Hemp (Cannabis sativa) is a sustainable plant known to produce various secondary metabolites with insecticidal properties, including terpenoids and flavonoids. The goal of this study was to assess the larvicidal activity of hemp leaf extract on mosquito larvae from both pyrethroid-susceptible (PS) and pyrethroid-resistant (PR) strains of Aedes aegypti. Another goal was to identify which components of the extract were responsible for any observed larvicidal activity. We found that a methanol extract of hemp leaves induced similar concentration-dependent larvicidal activity against PS (LC50: 4.4 ppm) and PR (LC50: 4.3 ppm) strains within 48 h. Partitioning of the leaf extract between methanol and hexane fractions revealed that full larvicidal activity was restricted to the methanol fraction. Analysis of this fraction by gas chromatography-mass spectrometry and nuclear magnetic resonance showed it to be dominated by cannabidiol (CBD). Larvicidal assays using authentic CBD confirmed this compound was primarily responsible for the toxicity of the hemp leaf extract against both strains. We conclude that hemp leaf extracts and CBD have the potential to serve as viable sources for the development of novel mosquito larvicides.

AC-DC Electropenetrography as a Tool to Quantify Probing and Ingestion Behaviors of the Yellow Fever Mosquito (Aedes aegypti) on Mice in Biocontainment.

A detailed understanding of mosquito probing and ingestion behaviors is crucial in developing novel interventions to interrupt the transmission of important human and veterinary pathogens, but these behaviors are difficult to observe as the mouthparts are inserted into the skin of the host. Electropenetrography (EPG) allows indirect observation, recording, and quantification of probing and ingestion behaviors of arthropods by visualizing the electrical waveform associated with these behaviors. The study of mosquito probing and ingestion behaviors has been limited to the use of human hands as host, which is not suitable for pathogen transmission studies. Mouse models of mosquito-borne diseases are a widely used and indispensable tool in this research, but previous attempts to use direct current EPG to study mosquito probing behaviors on mice have been unsuccessful. Accordingly, the present study used alternating or direct current (AC-DC) EPG to observe the ingestion behaviors of adult Aedes aegypti mosquitoes on a mouse host in real time under BSL-2 containment conditions with enhanced BSL-3 practices. Our results show that waveform families previously identified during Ae. aegypti probing and ingestion on human hands were observed using 100 mV of AC at an input resistance (Ri) of 107 Ohms (Ω) on CD1 mice. This work is a proof of concept for using mouse models for studying mosquito probing and ingestion behaviors with AC-DC EPG. In addition, these data show that the experimental setup used in these experiments is sufficient for conducting studies on mosquito probing and ingestion behaviors under BSL-2 containment conditions enhanced with BSL-3 practices. This work will serve as a foundation for using EPG to investigate the effects of pathogen infection on mosquito probing behaviors and to understand the real-time dynamics of pathogen transmission.

Erratum: AC-DC Electropenetrography as a Tool to Quantify Probing and Ingestion Behaviors of the Yellow Fever Mosquito (Aedes aegypti) on Mice in Biocontainment.

This corrects the article DOI: 10.30802/AALAS-CM-23-000037. When the above article was first published in the Vol 73 No 6 (December 2023) issue of Comparative Medicine, figure images were incorrectly associated with the figure legends. The correct version of this article has been reprinted in full in volume 74, issue 1 of the February issue of Comparative Medicine. The publisher apologizes for this error and any inconvenience caused.

Insecticidal and Synergistic Potential of Three Monoterpenoids against the Yellow Fever Mosquito, Aedes aegypti (Diptera: Culicidae), and the House Fly, Musca domestica (Diptera: Muscidae)

As resistance to the limited number of insecticides available for medical and veterinary pests becomes more widespread, there is an urgent need for new insecticides and synergists on the market. To address this need, we conducted a study to assess the toxicity of three monoterpenoids-carvone, menthone, and fenchone-in comparison to permethrin and methomyl against adults of two common pests: the yellow fever mosquito (Aedes aegypti) and the house fly (Musca domestica). We also examined the potential for these monoterpenoids to enhance the effectiveness of permethrin and methomyl when used together. Finally, we evaluated the ability of each monoterpenoid to inhibit acetylcholinesterase, comparing them to methomyl. While all three monoterpenoids performed relatively poorly as topical insecticides (LD50 > 4000 ng/mg on M. domestica; >6000 ng/mg on Ae. aegypti), they synergized both permethrin and methomyl as well as or better than piperonyl butoxide (PBO). Carvone and menthone yielded synergistic co-toxicity factors (23 and 29, respectively), which were each higher than PBO at 24 h. Currently, the mechanism of action is unknown. During preliminary testing, symptoms of acetylcholinesterase inhibition were identified, prompting further testing. Acetylcholinesterase inhibition did not appear to explain the toxic or synergistic effects of the three monoterpenoids, with IC50 values greater than 1 mM for all, compared to the 2.5 and 1.7 µM for methomyl on Aedes aegypti and Musca domestica, respectively. This study provides valuable monoterpenoid toxicity and synergism data on two pestiferous insects and highlights the potential for these chemistries in future pest control formulations.

Dopamine modulates odour learning in mosquitoes

Researchers have long been fascinated with figuring out how mosquitoes choose a host to feed on and how they can change their preferences. One important missing piece of the puzzle may be the way that mosquitoes learn different smells. Gabriella Wolff and colleagues from the University of Washington, USA, had previously found that yellow fever mosquitoes could form memories associated with odours related to their favourite food – blood – but not with odours that had nothing to do with their life history. These mosquitoes could be trained to avoid those odours by associating them with swatting. However, it was still unknown whether other mosquito species could learn to avoid the same odours and whether dopamine, a chemical messenger important for learning, could be responsible for this olfactory learning. To figure this out, the team set out to train four different species of mosquitoes.Wolff and colleagues exposed the mosquitoes to the scents of human skin, chicken feathers and rose oil. Two of the mosquito species feed on humans [the yellow fever mosquito (Aedes aegypti) and the Asian malaria mosquito (Anopheles stephensi)], another, the southern house mosquito (Culex quinquefasciatus), feeds on birds, and the elephant mosquito (Toxorhynchites amboinensis) dines on flowers. The team found that all the mosquito species had the strongest attraction to the scent of the hosts that they preferred to feed on.To test whether multiple mosquito species could learn to recognize the same odours, the team trained female mosquitoes from each species to avoid different scents by exposing them to scent components from human odours (octenol and hexanoic acid) or a flower scent component (linalool), regardless of whether they naturally feed on humans or flowers, and then they mimicked swatting at the insects to make the scent unattractive. The team then investigated each mosquito’s preference by putting them into a maze that provided them with a choice between either a host odour (octenol, hexanoic acid or linalool) or no scent. The team found that the mosquitoes learned to avoid the odours associated with their favourite food, but not other odours. For example, the yellow fever mosquito, which dines on people, learned to avoid octenol and hexanoic acid, but not linalool. Meanwhile, nectar-feeding elephant mosquitoes only learned to avoid linalool, but not octenol and hexanoic acid.To see whether these differences in species learning were due to the effects of dopamine, the researchers investigated where dopamine was localized in the brain of each of these species. Wolff and colleagues found that dopamine was localized in the antennal lobe and mushroom bodies – structures in the brain that are important for learning and memory. However, the dopamine was localized in different areas of these structures from species to species, which could be important for differences in their learning abilities. To see whether dopamine was necessary for this olfactory learning, the team also trained yellow fever mosquitoes and the Asian malaria mosquitoes, which feed on mammals, to avoid an odour associated with their preferred diets, and found that when they blocked the dopamine receptor, they eliminated their ability to learn to avoid the odour. Last, the team found that areas in the antennal lobe that had an increased response to these scents also had a raised level of dopamine.Learning the basis of how mosquitoes smell and the impact on their host preference is vital, as the insects can be responsible for the spread of some terrible diseases. The researchers highlight that being able to change dopamine inputs without having to change brain structure may give mosquitoes an evolutionary advantage and could be a way for mosquitoes to change their host preferences quickly.

The current and future distribution of the yellow fever mosquito (Aedes aegypti) on Madeira Island.

The Aedes aegypti mosquito is the main vector for several diseases of global importance, such as dengue and yellow fever. This species was first identified on Madeira Island in 2005, and between 2012 and 2013 was responsible for an outbreak of dengue that affected several thousand people. However, the potential distribution of the species on the island remains poorly investigated. Here we assess the suitability of current and future climatic conditions to the species on the island and complement this assessment with estimates of the suitability of land use and human settlement conditions. We used four modelling algorithms (boosted regression trees, generalized additive models, generalized linear models and random forest) and data on the distribution of the species worldwide and across the island. For both climatic and non-climatic factors, suitability estimates predicted the current distribution of the species with good accuracy (mean area under the Receiver Operating Characteristic curve = 0.88 ±0.06, mean true skill statistic = 0.72 ±0.1). Minimum temperature of coldest month was the most influential climatic predictor, while human population density, residential housing density and public spaces were the most influential predictors describing land use and human settlement conditions. Suitable areas under current climates are predicted to occur mainly in the warmer and densely inhabited coastal areas of the southern part of the island, where the species is already established. By mid-century (2041–2060), the extent of climatically suitable areas is expected to increase, mainly towards higher altitudes and in the eastern part of the island. Our work shows that ongoing efforts to monitor and prevent the spread of Ae. aegypti on Madeira Island will have to increasingly consider the effects of climate change.

Larvicidal Activity of Carbon Black against the Yellow Fever Mosquito Aedes aegypti.

Simple SummaryNanoparticles have previously shown potential to control mosquito vectors. The present study examined whether carbon black, an industrial source of carbon-based nanoparticles (CNPs), was toxic to larvae of the yellow fever mosquito (Aedes aegypti). We found that exposing the first developmental stages of mosquito larvae to a modified form of carbon black EMPEROR® 1800 (E1800), caused concentration-dependent mortality within 48 h of exposure; however, the development of larvae exposed to sub-lethal concentrations of E1800 was not disrupted. Analyses of E1800 suspensions suggest this carbon black forms CNPs that coalesce into larger aggregations. Microscopic observations of dead larvae showed the presence of CNP aggregations in the digestive tract and on external structures associated with swimming, breathing, and food uptake. Our results suggest carbon black is a source of CNPs that may have potential use for treating sources of standing water that mosquitoes use as breeding sites.The yellow fever mosquito Aedes aegypti is one of the deadliest animals on the planet because it transmits several medically important arboviruses, including Zika, chikungunya, dengue, and yellow fever. Carbon-based nanoparticles (CNPs) derived from natural sources have previously been shown to have toxic effects on mosquito larvae and offer a potential alternative to chemical insecticides such as pyrethroids, for which mosquitoes have evolved resistance. However, CNPs derived from industrial sources, such as carbon black, have not previously been evaluated as larvicides. Here, we evaluate the effects of a commercially-available carbon black, EMPEROR® 1800 (E1800), on mortality and development of pyrethroid-susceptible (PS) and pyrethroid-resistant (PR) strains of Ae. aegypti. We found that E1800 exhibited concentration-dependent mortality against 1st instar larvae of both strains within the first 120 h after exposure, but after this period, surviving larvae did not show delays in their development to adults. Physical characterization of E1800 suspensions suggests that they form primary particles of ~30 nm in diameter that fuse into fundamental aggregates of ~170 nm in diameter. Notably, larvae treated with E1800 showed internal accumulation of E1800 in the gut and external accumulation on the respiratory siphon, anal papillae, and setae, suggesting a physical mode of toxic action. Taken together, our results suggest that E1800 has potential use as a larvicide with a novel mode of action for controlling PS and PR mosquitoes.

Radio-frequency exposure of the yellow fever mosquito (A. aegypti) from 2 to 240 GHz.

Fifth generation networks (5G) will be associated with a partial shift to higher carrier frequencies, including wavelengths comparable in size to insects. This may lead to higher absorption of radio frequency (RF) electromagnetic fields (EMF) by insects and could cause dielectric heating. The yellow fever mosquito (Aedes aegypti), a vector for diseases such as yellow and dengue fever, favors warm climates. Being exposed to higher frequency RF EMFs causing possible dielectric heating, could have an influence on behavior, physiology and morphology, and could be a possible factor for introduction of the species in regions where the yellow fever mosquito normally does not appear. In this study, the influence of far field RF exposure on A. aegypti was examined between 2 and 240 GHz. Using Finite Difference Time Domain (FDTD) simulations, the distribution of the electric field in and around the insect and the absorbed RF power were found for six different mosquito models (three male, three female). The 3D models were created from micro-CT scans of real mosquitoes. The dielectric properties used in the simulation were measured from a mixture of homogenized A. aegypti. For a given incident RF power, the absorption increases with increasing frequency between 2 and 90 GHz with a maximum between 90 and 240 GHz. The absorption was maximal in the region where the wavelength matches the size of the mosquito. For a same incident field strength, the power absorption by the mosquito is 16 times higher at 60 GHz than at 6 GHz. The higher absorption of RF power by future technologies can result in dielectric heating and potentially influence the biology of this mosquito.

Re-Analysis of 16S rRNA Gene Sequence Data Sets Uncovers Disparate Laboratory-Specific Microbiomes Associated with the Yellow Fever Mosquito (Aedes aegypti).

Host-microbiome dynamics occurring in the yellow fever mosquito (Aedes aegypti) contribute to host life history traits, and particular bacterial taxa are proposed to comprise a "core" microbiota that influences host physiology. Laboratory-based studies are frequently performed to investigate these processes; however, experimental results are often presumed to be generalizable across laboratories, and few efforts have been made to independently reproduce and replicate significant findings. A recent study by Muturi et al. (FEMS Microbiol Ecol 95 (1):213, 2019) demonstrated the food source imbibed by laboratory-reared adult female mosquitoes significantly impacted the host-associated microbiota-a foundational finding in the field of mosquito biology worthy of independent evaluation. Here, we coalesce these data with two additional mosquito-derived 16S rRNA gene sequence data sets using a unifying bioinformatics pipeline to reproduce the characterization of these microbiota, test for a significant food source effect when independent samples were added to the analyses, assess whether similarly fed mosquito microbiomes were comparable across laboratories, and identify conserved bacterial taxa. Our pipeline characterized similar microbiome composition and structure from the data published previously, and a significant food source effect was detected with the addition of independent samples, increasing the robustness of this previously discovered component of mosquito biology. However, distinct microbial communities were identified from similarly fed but independently reared mosquitoes, and surveys across all samples did not identify conserved bacterial taxa. These findings demonstrated that while the main effect of the food source was supported, laboratory-specific conditions may produce inherently differential microbiomes across independent laboratory environments.

Nonlethal Effects of Pesticides on Web-Building Spiders Might Account for Rapid Mosquito Population Rebound after Spray Application

Spiders are important population regulators of insect pests that spread human disease and damage crops. Nonlethal pesticide exposure is known to affect behavior of arthropods. For spiders such effects include the inability to repair their webs or capture prey. In this study, nonlethal exposure of Mabel’s orchard spider (Leucauge argyrobapta) to the synthetic pyrethroid permethrin, via web application, interfered with web reconstruction and mosquito capture ability for 1–3 days. The timing of this loss-of-predator ecosystem function corresponds to the rapid population rebound of the yellow fever mosquito (Aedes aegypti) following insecticide application to control arbovirus epidemics. We suggest this temporal association is functional and propose that follow-up study be conducted to evaluate its significance.

Drug Resistance in Filarial Parasites Does Not Affect Mosquito Vectorial Capacity.

Parasite drug resistance presents a major obstacle to controlling and eliminating vector-borne diseases affecting humans and animals. While vector-borne disease dynamics are affected by factors related to parasite, vertebrate host and vector, research on drug resistance in filarial parasites has primarily focused on the parasite and vertebrate host, rather than the mosquito. However, we expect that the physiological costs associated with drug resistance would reduce the fitness of drug-resistant vs. drug-susceptible parasites in the mosquito wherein parasites are not exposed to drugs. Here we test this hypothesis using four isolates of the dog heartworm (Dirofilaria immitis)—two drug susceptible and two drug resistant—and two vectors—the yellow fever mosquito (Aedes aegypti) and the Asian tiger mosquito (Ae. albopictus)—as our model system. Our data indicated that while vector species had a significant effect on vectorial capacity, there was no significant difference in the vectorial capacity of mosquitoes infected with drug-resistant vs. drug-susceptible parasites. Consequently, contrary to expectations, our data indicate that drug resistance in D. immitis does not appear to reduce the transmission efficiency of these parasites, and thus the spread of drug-resistant parasites in the vertebrate population is unlikely to be mitigated by reduced fitness in the mosquito vector.

Antipredatory Responses of Mosquito Pupae to Non-Lethal Predation Threat—Behavioral Plasticity Across Life-History Stages

Antipredatory behavioral responses tend to be energetically expensive, and prey species thus need to resolve trade-offs between these behaviors and other activities such as foraging and mating. While these trade-offs have been well-studied across taxa, less is known about how costs and benefits vary in different life-history contexts, and associated consequences. To address this question, we compared responses of the yellow fever mosquito (Aedes aegypti [Diptera: Culicidae]) to predation threat from guppy (Poecilia reticulata [Cyprinodontiformes: Poeciliidae]) across two life-history stages-larvae (data from previous study) and pupae (from this study). Pupae are motile but do not feed and are comparable to larvae in terms of behavior. To understand how physiological costs affect the threat sensitivity of pupae, we used sex (with size as a covariate) as a proxy for stored energy reserves, and quantified movement and space use patterns of male (small-sized) and female (large-sized) pupae when exposed to predation threat. We found that pupae did not alter movement when exposed to predator cues but instead altered spatial use by spending more time at the bottom of the water column. We found no effect of pupa sex (or size) on the behavioral responses we measured. We conclude that pupa behavior, both antipredatory and otherwise, is primarily targeted at minimizing energy expenditure, as compared with larval behavior, which appears to balance energy expenditure between the opposing pressures of foraging and of avoiding predation. We suggest that antipredatory defenses in metamorphosing prey are modulated by varying energetic trade-offs associated with different life-history stages.

Impacts of Dietary Nutritional Composition on Larval Development and Adult Body Composition in the Yellow Fever Mosquito (Aedes aegypti).

Simple SummaryThe mosquito Aedes aegypti (Ae. aegypti) is responsible for the spread of viruses such as Zika and Dengue. The nutritional environment of immature Ae. aegypti is important for development of larvae and resulting adult mosquitoes. Larval mosquitoes with inadequate nutrition can result in developmental failure or impact the size and reproductive ability of adults. Understanding the nutritional requirements of larval mosquitoes allows us to optimize lab reared mosquitoes and identify new targets for mosquito control. We tested the effect of diets with different ratios of protein to carbohydrates on the life history traits of Ae. aegypti. Each diet was composed of autolyzed Brewer’s yeast (protein), and/or rice flour (carbohydrates). Larvae fed a medium-low protein diet had the shortest pupation time. As adults, the medium-low protein dietary group also had the longest wing lengths, highest weights, and increased lipid stores compared to the adults in all other dietary groups. These findings indicate that both carbohydrates and protein are essential components of Aedes aegypti larval diets. However, Ae. aegypti larvae fed a diet rich in carbohydrates and lower in protein seem to flourish as long as they receive enough dietary protein to fulfill basic biochemical requirements for growth and development.Background: the mosquito Aedes aegypti (Ae. aegypti) is an important vector of arboviruses, including Zika, Dengue, and Chikungunya. The dietary requirements of larval Ae. aegypti are not well understood and likely impact developmental and physiological parameters knowledge of which could be important for vector control. This study examines the effects nutrition has on growth and development of larval Ae. aegypti of laboratory-reared Rockefeller strain mosquitoes. Methods: mosquito larvae were split into five feeding groups with diets providing different ratios of protein and carbohydrates. Each group received autolyzed Brewer’s yeast (AY - high-protein), and/or rice flour (RF—high-carbohydrate). The groups were monitored to record larval developmental times, adult sizes and nutritional stores. Results: the 100% AY group failed to pupate, suggesting the AY alone is either lacking in critical nutrients or is toxic at higher concentrations. The 100% RF group resulted in the smallest adults that took the longest time to reach pupation. Of the remaining groups, the 25% AY/75% RF (Med–low) diet yielded adult mosquitoes with highest average weight, wing length, and lipid stores relative to the other diets. Conclusions: the dietary requirements for development, body size, and nutrient stores of Ae. aegypti mosquitoes appear to be dependent on a relatively low but essential proportion of dietary protein to carbohydrates to achieve optimal developmental outcomes.

RNA Interference Is Enhanced by Knockdown of double-stranded RNases in the Yellow Fever Mosquito Aedes Aegypti.

RNA interference (RNAi) techniques are being developed for a range of pest insect control technologies, including the sterile insect technique (SIT) and double-stranded RNA (dsRNA)-based insecticides. In SIT applications, where >99% of the released males should be sterile to meet industry standards, the efficiency of RNAi will need to be improved for many insect species if this technology is to be adopted. Endogenous dsRNases can impede dsRNA delivery in some insects, and, here, we investigated whether dsRNases in the midgut could limit RNAi efficacy in the mosquito Aedes aegypti. Ten putative dsRNases were identified in the Ae. aegypti genome, with two highly expressed in the midguts of larvae. Using an ex vivo assay, we observed that dsRNA was rapidly degraded within the mosquito larva’s gut. Double-stranded RNA targeting these two dsRNases, when fed to the larvae, effectively reduced gut dsRNase activity. When these dsRNase-specific dsRNAs were co-delivered with dsRNA targeting a cyan fluorescent protein (CFP) reporter gene, greater knockdown of CFP fluorescence was observed. These results suggest that inhibiting dsRNase activity could enable the implementation of RNAi-based mosquito control methods.

Influences of a Prolific Gut Fungus (Zancudomyces culisetae) on Larval and Adult Mosquito (Aedes aegypti)-Associated Microbiota.

Adult mosquitoes inherit a bacterial community from larvae via transstadial transmission, an understudied process that may influence host-microbe interactions. Microbes contribute to important host life history traits, and analyzing transmitted microbial communities, the interrelationship between larval and adult-associated microbiota, and factors influencing host-microbe relationships provides targets for research. During its larval stage, the yellow fever mosquito (Aedes aegypti) hosts the trichomycete gut fungus Zancudomyces culisetae, and fungal colonization coincides with environmental perturbations in the digestive tract microecosystem. Natural populations are differentially exposed to fungi, thereby potentially harboring distinct microbiota and experiencing disparate host-microbe interactions. This study's objectives were to characterize larval and initial adult microbiomes, investigate variation in diversity and distribution of microbial communities across individuals, and assess whether larval fungal colonization impacted microbiomes at these developmental stages. Laboratory-based fungal infestation assays, sequencing of 16S rRNA gene amplicons, and bacterial load quantification protocols revealed that initial adult microbiomes varied in diversity and distribution. Larval fungal colonization had downstream effects on initial adult microbiomes, significantly reducing microbial community variation, shifting relative abundances of certain bacterial families, and influencing transstadial transmission outcomes of particular genera. Further, abundances of several families consistently decreased in adults relative to levels in larvae, possibly reflecting impacts of host development on specific bacterial taxa. These findings demonstrated that a prolific gut fungus impacted mosquito-associated microbiota at two developmental stages in an insect connected with global human health.IMPORTANCE Mosquitoes are widespread vectors of numerous human pathogens and harbor microbiota known to affect host phenotypic traits. However, little research has directly investigated how bacterial communities associated with larvae and adults are connected. We characterized whole-body bacterial communities in mosquito larvae preceding pupation and in newly emerged adults, and investigated whether a significant biotic factor, fungal colonization of the larval hindgut, impacted these microbiomes. Results showed that fungal colonization reduced microbial community variation across individuals and differentially impacted the outcomes of transstadial transmission for certain bacterial genera, revealing downstream effects of the fungus on initial adult microbiomes. The importance of our research is in providing a thorough comparative analysis of whole-body microbiota harbored in larvae and adults of the yellow fever mosquito (Aedes aegypti) and in demonstrating the important role a widespread gut fungus played in a host-associated microbiome.

Can beets beat mosquitoes?

If you’ve smelled the musty odor of moist soil or the air after a rainstorm, you’ve smelled geosmin, a terpene produced by soil bacteria and cyanobacteria (blue-green algae). Although scientists don’t know why humans have developed such a sensitivity for the scent, it seems some insects can also detect it. In Sweden, the US, and Brazil, researchers have now found that the yellow-fever mosquito Aedes aegypti is attracted to geosmin. The scientists demonstrated that the odorant could be useful in mosquito traps that help control the spread of disease (Curr. Biol. 2019, DOI: 10.1016/j.cub.2019.11.002). In 2012, Marcus C. Stensmyr of Lund University was part of a group that found that the common fruit fly Drosophila melanogaster sniffs out and avoids geosmin because the compound is produced by molds that make fruit unsuitable feeding and breeding sites (Cell 2012, DOI: 10.1016/j.cell.2012.09.046). Because of the group’s finding in fruit flies, Nadia Melo,

Genetic diversity of laboratory strains and implications for research: The case of Aedes aegypti.

The yellow fever mosquito (Aedes aegypti), is the primary vector of dengue, Zika, and chikungunya fever, among other arboviral diseases. It is also a popular laboratory model in vector biology due to its ease of rearing and manipulation in the lab. Established laboratory strains have been used worldwide in thousands of studies for decades. Laboratory evolution of reference strains and contamination among strains are potential severe problems that could dramatically change experimental outcomes and thus is a concern in vector biology. We analyzed laboratory and field colonies of Ae. aegypti and an Ae. aegypti-derived cell line (Aag2) using 12 microsatellites and ~20,000 SNPs to determine the extent of divergence among laboratory strains and relationships to their wild relatives. We found that 1) laboratory populations are less genetically variable than their field counterparts; 2) colonies bearing the same name obtained from different laboratories may be highly divergent; 3) present genetic composition of the LVP strain used as the genome reference is incompatible with its presumed origin; 4) we document changes in two wild caught colonies over ~16 generations of colonization; and 5) the Aag2 Ae. aegypti cell line has experienced minimal genetic changes within and across laboratories. These results illustrate the degree of variability within and among strains of Ae. aegypti, with implications for cross-study comparisons, and highlight the need of a common mosquito repository and the implementation of strain validation tools.

RNA-Seq Comparison of Larval and Adult Malpighian Tubules of the Yellow Fever Mosquito Aedes aegypti Reveals Life Stage-Specific Changes in Renal Function.

Introduction: The life history of Aedes aegypti presents diverse challenges to its diuretic system. During the larval and pupal life stages mosquitoes are aquatic. With the emergence of the adult they become terrestrial. This shifts the organism within minutes from an aquatic environment to a terrestrial environment where dehydration has to be avoided. In addition, female mosquitoes take large blood meals, which present an entirely new set of challenges to salt and water homeostasis.Methods: To determine differences in gene expression associated with these different life stages, we performed an RNA-seq analysis of the main diuretic tissue in A. aegypti, the Malpighian tubules. We compared transcript abundance in 4th instar larvae to that of adult females and analyzed the data with a focus on transcripts that encode proteins potentially involved in diuresis, like water and solute channels as well as ion transporters. We compared our results against the model of potassium- and sodium chloride excretion in the Malpighian tubules proposed by Hine et al. (2014), which involves at least eight ion transporters and a proton-pump.Results: We found 3,421 of a total number of 17,478 (19.6%) unique transcripts with a P < 0.05 and at least a 2.5 fold change in expression levels between the two groups. We identified two novel transporter genes that are highly expressed in the adult Malpighian tubules, which have not previously been part of the transport model in this species and may play important roles in diuresis. We also identified candidates for hypothesized sodium and chloride channels. Detoxification genes were generally higher expressed in larvae.Significance: This study represents the first comparison of Malpighian tubule transcriptomes between larval and adult A. aegypti mosquitoes, highlighting key differences in their renal systems that arise as they transform from an aquatic filter-feeding larval stage to a terrestrial, blood-feeding adult stage.