Abstract The fragments produced by digestion of yeast phenylalanine transfer RNA (tRNAphe) with Taka-Diastase ribonuclease T1 have been resolved on anion exchange columns and characterized. The 3'-terminal sequence has been confirmed to be CpApCpC, and the tetranucleotide TpψpCpGp, concluded previously to be a sequence common to tRNAs, is present also in this RNA. The results of the present work and those described previously on degradation with pancreatic ribonuclease are in mutual agreement and, taken together, permit the derivation of longer oligonucleotide sequences: the latter account for 55 out of 76 nucleotide residues of tRNAphe. The largest oligonucleotide isolated is the dodecanucleotide Ap2'OMeCpUp2'OMeGpApApYp-Apψp5MeCpUpGp; this oligonucleotide contains the presumed anticodon, 2'OMeGpApA.