In this work, we demonstrate a homemade 20/400 µm polarization-maintaining ytterbium-doped fiber (PMYDF) with a stress birefringence value of 4.2 × 10−4. The core concentrations of Yb2O3 and Al2O3 were 0.79 wt% and 0.83 wt% respectively. The cladding absorption at 976 nm was measured to be 1.5 dB/m. The PMYDF exhibited a polarization extinction ratio (PER) of 18 dB over meter-long fiber lengths, favoring linearly polarized laser amplification. By applying the PMYDF in the amplifier, an output power of 1247 W was achieved with a 3 dB spectral linewidth of 87 pm under the pumping of 976 nm lasers, corresponding to a slope efficiency of 83.1 %. With a high polarization capability, this 20/400 µm PMYDF exhibits great potential for application in high-power, narrow linewidth, linearly polarized fiber lasers.