Yamagata Prefecture Research Articles

Recovery time is associated with the onset of cardiovascular disease in Japanese patients undergoing maintenance hemodialysis.

Cardiovascular disease (CVD) is the leading cause of mortality in patients undergoing maintenance hemodialysis (HD), with various reported risk factors. Recovery time (RT) is a valuable indicator of post-dialysis fatigue. However, the association between RT and the onset of CVD remains unexplored. As such, this study aimed to determine the effect of RT on the onset of CVD. Data from 620 patients undergoing maintenance HD at Yabuki Hospital and 3 related facilities (Yamagata Prefecture, Japan) as of December 31, 2020, were retrospectively analyzed. Patients were asked to respond to the question "How long does it take you to recover from a dialysis session?"; the response was defined as RT. The analysis was performed by categorizing patients into 2 groups according to RT: short RT (< 2h); and long RT (≥ 2h). The primary outcome was major adverse cardiovascular events (MACE). Secondary outcomes included all-cause death and hospitalization. During the 24-month follow-up, 70 (11.3%) patients developed MACE. The long RT group exhibited a higher incidence of MACE; multivariate analysis revealed that age and long RT were associated with the onset of MACE. All-cause death was observed in 45 (7.3%) patients, with no significant difference between the 2 groups. Hospitalization occurred in 252 (40.6%) patients and was more frequent in the long RT group. Multivariate analysis revealed that age, sex, duration of HD, intact parathyroid hormone level, and long RT were associated with hospitalization. Long RT was an independent risk factor for the onset of CVD.

Study Profile of the Tsuruoka Metabolomics Cohort Study (TMCS).

The Tsuruoka Metabolomics Cohort Study (TMCS) is an ongoing population-based cohort study being conducted in the rural area of Yamagata Prefecture, Japan. This study aimed to enhance the precision prevention of multi-factorial, complex diseases, including non-communicable and aging-associated diseases, by improving risk stratification and prediction measures. At baseline, 11,002 participants aged 35-74 years were recruited in Tsuruoka City, Yamagata Prefecture, Japan, between 2012 and 2015, with an ongoing follow-up survey. Participants underwent various measurements, examinations, tests, and questionnaires on their health, lifestyle, and social factors. This study uses an integrative approach with deep molecular profiling to identify potential biomarkers linked to phenotypes that underpin disease pathophysiology and provide better mechanistic insights into social health determinants. The TMCS incorporates multi-omics data, including genetic and metabolomic analyses of 10,933 participants, and comprehensive data collection ranging from physical, psychological, behavioral, and social to biological data. The metabolome is used as a phenotypic probe because it is sensitive to changes in physiological and external conditions. The TMCS focuses on collecting outcomes for cardiovascular disease, cancer incidence and mortality, disability and functional decline due to aging and disease sequelae, and the variation in health status within the body represented by omics analysis that lies between exposure and disease. It contains several sub-studies on aging, heated tobacco products, and women's health. This study is notable for its robust design, high participation rate (89%), and long-term repeated surveys. Moreover, it contributes to precision prevention in Japan and East Asia as a well-established multi-omics platform.

Participant Selection from the General Japanese Population for Pulmonary Function Tests Using a Questionnaire on Symptoms and Smoking Habits during Annual Health Checkups: The Yamagata-Takahata Study.

Objective Pulmonary function tests are essential for diagnosing respiratory diseases, such as chronic obstructive pulmonary disease (COPD), but are typically not performed in Japan during annual health checkups, which hinders the early diagnosis of respiratory diseases. Methods Individuals who agreed to participate in the Yamagata-Takahata study during medical checkups in Takahata (Yamagata Prefecture, Japan) in 2011 were examined. We interviewed 669 participants (49.0% men; mean age, 67.7 years old) regarding their respiratory symptoms and smoking habits and performed pulmonary function tests during the study. Results Based on pulmonary function test results, 141 participants had pulmonary dysfunction, and 115 had obstructive pulmonary dysfunction. The risk of respiratory dysfunction, particularly obstructive respiratory dysfunction, was examined by referring to a questionnaire tool for an early COPD diagnosis. The associations between age, the smoking history, respiratory symptoms, and obstructive respiratory dysfunction were evaluated. Obstructive respiratory dysfunction was found in 17.6% of participants ≥50 years old and 19.5% ≥60 years old, 30.3% had a smoking history, and 32.8% had respiratory symptoms. Furthermore, the participants with multiple factors had a higher probability of obstructive respiratory dysfunction. Conclusion Subjects with obstructive pulmonary dysfunction are expected to be efficiently identified by extracting individuals by age and smoking habit and through a respiratory symptom questionnaire, although pulmonary function tests cannot be performed for all individuals during health checkups.

Comparing the Cultural Landscapes of Japan and China: An Example of Preserved Food Production in Yamagata Prefecture, Japan, and Sichuan Province, China

This study examines the development of cultural landscape research in Japan and China, focusing on the underrepresentation of studies addressing cultural landscapes associated with food production, which is particularly evident in China. Employing a case study methodology, this research investigates the production of pickles in Sichuan Province, China, and Yamagata Prefecture, Japan. This study elucidates the components and characteristics of cultural landscapes related to pickle production in these regions. It was found that residential and transportation conditions significantly influence the composition and landscape patterns of these cultural landscapes. The integration of food production practices with local dietary systems and community awareness is emphasized in this research. The findings offer insights and serve as a reference for understanding and preserving food production cultural landscapes in other countries and regions.

Visit to valuable water springs (144) Valuable springs in Akita and Yamagata prefectures — including springs revisited

Visit to valuable water springs (144) Valuable springs in Akita and Yamagata prefectures — including springs revisited

Examining Traditional Food Processing Landscapes from the Perspective of Cultural Landscapes: Pickle-Making in Yamagata Prefecture, Japan

The cultural landscapes of living and living industries in Asia are changing and disappearing, and this is currently under-documented and under-discussed in the international publishing field. This study takes pickle-making in Yamagata Prefecture, Japan, as the research subject, clarifies the constituent elements and characteristics of the cultural landscapes of pickle-making in Yamagata, and analyses the formation and change of the local landscapes from the point of view of the food-processing and production activities. The elements of the cultural landscapes of pickle-making in Yamagata Prefecture were classified as follows: landscapes of cultivation sites, landscapes of processing sites, landscapes of sales sites, landscapes of cooking and eating spaces, and landscapes of special festivals. It was found that the cultural landscapes of pickle-making in Yamagata Prefecture can play a role in supporting and maintaining biodiversity and ecological balance within the region and that they are highly recognizable and inherited. The elements of the cultural landscape of pickle-making are rich and diverse, and the carriers of dependence are not fixed. Special celebrations and festivals constitute important intangible elements of food production behavior and become the spiritual connotation of its cultural landscape. The results of this study are useful in supplementing the current system of cultural landscapes in Japan and the gaps in existing research. It is also conducive to enhancing the understanding of Japanese food culture in the international publishing field and the influence of productive cultural landscapes in Japan as well as in Asia.

Evaluation of carbon emission reduction effect of Cryptomeria japonica stand management in Yamagata Prefecture, Japan

Planted forests are in the utilisation phase in Japan, contributing to the increased use of timber and the realisation of carbon neutrality. We simulated and compared the carbon emission reduction effects of three forest management scenarios, including a no final cutting scenario at two geographical scales, in the context of a privately owned Cryptomeria japonica planted forest in Tsuruoka City, Yamagata Prefecture, Japan. We adopted two substitution approaches: the status quo approach and the dynamic approach in line with Japan’s nationally determined contribution. We used hypothetical stands over three rotation periods of 150 years at the stand level, and a harvest schedule of 120 years at the regional level. The harvesting scenarios based on the status quo substitution approach reduced carbon emissions more than the no final cutting scenario for current wood usage at the study site, although those based on the dynamic substitution approach reduced carbon emissions as much as the no final cutting scenario during the first 30 years. Sustainable timber production from forests may reduce carbon emissions as much as unharvested forests until 2050, indicating that the substitution effects of harvested wood products could have a significant impact on the climate change mitigation effect arising from timber.

Current State of Japanese Cedar (Cryptomeria japonica D. Don) Pollen Information and Future Directions for Its Airborne Allergen Determination and Improved Pollen Monitoring

About 40% of cedar pollinosis patients living in the Yamagata Prefecture showed pollinosis symptoms before the first day of the pollen season, which was determined by Durham samplers, the standard sampler for pollen information in Japan. The amount of Cry j 1 (major cedar pollen allergen) per cedar pollen is reported to be six pg. This amount is difficult to measure using the ELISA method, so we applied the highly sensitive ESR radical immunoassay method to measure the allergen; now we can provide information for sensitive patients. It revealed that Cry j 1 exists in orbicles and tapetum. It is presumed that it is smaller than pollen, so it comes from a place where cedar are already in bloom. It is desirable to obtain real-time information on an hourly basis. Currently, information from automatic cedar pollen monitors is becoming main-stream. However, this monitor may count during snowfalls, Asian dust flying, etc., even when there was no apparent pollen examined with a microscope. This paper describes the current status of automatic cedar pollen monitors, their usefulness, and their advantages and disadvantages in comparison with results obtained by other methods of measurement. Lastly, the paper describes expectations for cedar pollen information in the future.

Characteristics of Food Procurement from the Perspective of Labor Distribution within Households:A Case Study of Koesawa Village in Tsuruoka City, Yamagata Prefecture

Characteristics of Food Procurement from the Perspective of Labor Distribution within Households:A Case Study of Koesawa Village in Tsuruoka City, Yamagata Prefecture

Associations between subjective well-being, number of teeth, and self-rated mastication in Japanese adults: A cross-sectional study.

This cross-sectional study aimed to investigate the risk factors for low levels of subjective well-being (SWB) in the general population of Japan, specifically, the impact of the number of teeth and self-rated mastication. The surveyed population consisted of individuals aged between 40 and 79 years from Yamagata prefecture, Japan. A postal self-administered questionnaire survey of respondents lifestyles, medical history, oral health, and dietary intake, was conducted from 2017 to 2021. We included 6846 participants to confirm the independent associations between SWB and several parameters using multivariate ordinal logistic regression analysis to estimate adjusted odds ratios and 95% confidence intervals. Individuals with < 20 teeth and poor mastication ability had a 1.3-fold risk for low levels of SWB compared with individuals with ≥ 20 teeth with good mastication ability (adjusted odds ratios = 1.300, 95% confidence intervals = 1.043-1.621, P = .020). There were no differences between individuals with good mastication ability, regardless of the number of remaining teeth. Our study emphasizes not only the importance of having ≥ 20 teeth and good mastication ability for high levels of SWB but also the importance of restoring mastication ability using some form of prosthesis, to facilitate a high level of SWB when the number of remaining teeth is < 20.

Association of health behaviors, dietary habits, and oral health with weight gain after 20years of age in community-dwelling Japanese individuals aged 40years and older: a cross-sectional study.

This cross-sectional study investigated the factors associated with weight gain ≥ 10kg after 20years of age in the general Japanese population, with a focus on the number of teeth. We included individuals aged ≥ 40years from Yamagata prefecture, Japan from 2017-2021. A postal survey was conducted using a self-administered questionnaire; 5,940 participants were included in the final analysis. The questionnaire included items on lifestyle factors, medical history, physical and mental conditions, oral health, and dietary intake. A multivariate logistic regression analysis was used to identify independent associations between weight gain ≥ 10kg after 20years of age and various parameters; adjusted odds ratios (ORs) and 95% confidence intervals (CIs) were estimated. Less than 20 teeth, male sex, drinking habit frequency, eating very fast or fast, and a higher frequency of eating-away-from-home were significant factors associated with weight gain ≥ 10kg after 20years of age; individuals with < 20 versus > 20 teeth exhibited a 1.35-fold higher OR (95% CI 1.15-1.59; p < 0.01). Our results suggest that having < 20 teeth may affect weight gain ≥ 10kg after 20years of age. However, owing to the cross-sectional study design, causality could not be determined. Therefore, maintaining healthy lifestyle behaviors to avoid tooth loss may also affect weight gain ≥ 10kg after 20years of age. Having < 20 teeth has the potential to affect long-term weight gain after 20years of age.

Attempts to isolate genes that determine the good taste on landrace edamame soybeans

Dadachamame is the common name for edamame soybean [Glycine max L.(Merr.)] cultivars, ‘Shirayama’, ‘Kanro’ and ‘Oura’, which have been established in Tsuruoka city, Yamagata prefecture in northern Japan since the late Edo era. Dadachamame has a good taste and special aroma. Although it is known that the good taste of Dadachamame is caused by the higher levels of sucrose and free amino acids based on the comparative analysis of chemical components in the seeds of Dadachamame and other soybean cultivars, details on the genetic level are unclear. To understand the good taste of Dadachamame on the genetic level, in this study, we developed an F2 population derived from a cross between the Dadachamame cultivar ‘Shirayama’ and the soybean cultivar ‘Enrei’. In the early development stage, Shirayama seeds had about 2-fold higher accumulation levels of sucrose compared with Enrei seeds. Sucrose content and genotype of the F2 population suggested that the quantitative trait locus (QTL) for high sucrose accumulation is located on the long arm of chromosome 12. The genes responsible for determining the sweetness of Shirayama may be present on chromosome 12. Using the lines developed from crosses between Dadachamame and soybean cultivars in future studies, we will attempt to identify the genes responsible for QTL that control for the good taste in specific Dadachamame seeds, and discuss the mechanisms for determining the good taste of Dadachamame to improve the palatability of edamame. Furthermore, we have developed mutant edamame populations that can be used for edamame breeding. Since mutant soybean populations have already been developed, meticulous soybean breeding will be possible in the future for creating the desired characteristics for the final soybean products.

Time-varying overdispersion of SARS-CoV-2 transmission during the periods when different variants of concern were circulating in Japan

Japan has implemented a cluster-based approach for coronavirus disease 2019 (COVID-19) from the pandemic’s beginning based on the transmission heterogeneity (overdispersion) of severe acute respiratory coronavirus 2 (SARS-CoV-2). However, studies analyzing overdispersion of transmission among new variants of concerns (VOCs), especially for Omicron, were limited. Thus, we aimed to clarify how the transmission heterogeneity has changed with the emergence of VOCs (Alpha, Delta, and Omicron) using detailed contact tracing data in Yamagata Prefecture, Japan. We estimated the time-varying dispersion parameter ({k}_{t}) by fitting a negative binomial distribution for each transmission generation. Our results showed that even after the emergence of VOCs, there was transmission heterogeneity of SARS-CoV-2, with changes in {k}_{t} during each wave. Continuous monitoring of transmission dynamics is vital for implementing appropriate measures. However, a feasible and sustainable epidemiological analysis system should be established to make this possible.

Image Segmentation-Based Bicycle Riding Side Identification Method

This paper attempts to identify which side of the road a bicycle is currently riding on using a common camera for realizing an advanced bicycle navigation system and bicycle riding safety support system. To identify the roadway area, the proposed method performs semantic segmentation on a front camera image captured by a bicycle drive recorder or smartphone. If the roadway area extends from the center of the image to the right, the bicyclist is riding on the left side of the roadway (i.e., the correct riding position in Japan). In contrast, if the roadway area extends to the left, the bicyclist is on the right side of the roadway (i.e., the incorrect riding position in Japan). We evaluated the accuracy of the proposed method on various road widths with different traffic volumes using video captured by riding bicycles in Tsuruoka City, Yamagata Prefecture, and Saitama City, Saitama Prefecture, Japan. High accuracy (>80%) was achieved for any combination of the segmentation model, riding side identification method, and experimental conditions. Given these results, we believe that we have realized an effective image segmentation-based method to identify which side of the roadway a bicycle riding is on.

Another advantage of multi-locus variable-number tandem repeat analysis that can putatively subdivide enterohemorrhagic Escherichia coli O157 strains into clades by maximum a posteriori estimation.

Enterohemorrhagic Escherichia coli O157 (O157) strains can be subdivided into clades based on their single-nucleotide polymorphisms, but such analysis using conventional methods requires intense effort by laboratories. Although multi-locus variable-number tandem repeat analysis (MLVA), which can be performed with low laboratory burden, has been used as a molecular epidemiological tool, it has not been evaluated whether MLVA can be used the clade subdivision of O157 strains like it can for that of other pathogenic bacteria. This study aimed to establish a method for subdividing O157 strains into clades using MLVA data. The standardized index of association, ISA, for O157 strains isolated in Chiba prefecture, Japan (Chiba isolates) revealed the presence of unique tandem repeat patterns in each major clade (clades 2, 3, 7, 8, and 12). A likelihood database of tandem repeats for these clades was then constructed using the Chiba isolates, and a formula for maximum a posteriori (MAP) estimation was constructed. The ratio of the number of O157 strains putatively subdivided into a clade by MAP estimation from MLVA data relative to the number of O157 strains subdivided using single-nucleotide polymorphism analysis (designated as the concordance ratio [CR]) was calculated using the Chiba isolates and O157 strains isolated in Yamagata prefecture (Yamagata isolates). The CRs for the major Chiba and Yamagata isolate clades, other than clade 2, were 89%-100%. Although the CR for clade 2 Chiba isolates was >95%, that of the Yamagata isolates was only 78.9%. However, these clade 2 CRs were not significantly different from one another, indicating that clade 2 strains can be subdivided correctly by MAP estimation. In conclusion, this study expands the utility of MLVA, previously applied predominantly for molecular epidemiological analysis, into a low-laboratory-burden tool for subdividing O157 strains into phylogenetic groups.

Visualization of the coronavirus infectious disease 2019 pandemic in Yamagata Prefecture using the spatio-temporal three-dimensional map

Visualization of the coronavirus infectious disease 2019 pandemic in Yamagata Prefecture using the spatio-temporal three-dimensional map

Influence of vegetation on occurrence and color of snow algal blooms in Mt. Gassan, Yamagata Prefecture, Japan

ABSTRACT Snow algae are photosynthetic microbes growing on melting snow surfaces, the blooms of which are visible on alpine snowpacks in Japan during the melting season. We characterized the seasonal and altitudinal variations in algal blooms on Mount Gassan, Japan, to assess the influence of vegetation on the algal bloom. From May to July in 2019, we collected colored snow from lower deciduous forest to the upper alpine areas. In the lower forest area, chlorophyll-a concentration in colored snow samples increased concomitantly with deciduous tree budburst, whereas in the alpine area, chlorophyll-a increased from June to July, although at lower levels than in the forest area. The absorption spectra of algal pigments extracted from samples differed among study sites and seasons, with those obtained from forest sites mainly characterized by absorption of chlorophylls and primary carotenoids, whereas those from alpine sites showed an intense secondary carotenoid peak throughout the study period. Chemical solute analyses revealed a general abundance of phosphate in snow, which was significantly higher in forest than in alpine sites and positively correlated with chlorophyll-a concentration. These findings suggest that nutrient supply from the forest canopy largely controls the appearance of colored snow in this area.

Tourism Application Considering Waiting Time

Currently, the proportion of independent travel is increasing in Japan. Therefore, earlier studies supporting itinerary planning have been presented. However, these studies have only insufficiently considered rural tourism. For example, tourist often use public transportation during trips in rural areas, although it is often difficult for a tourist to plan an itinerary for public transportation. Even if an itinerary can be planned, it will entail long waiting times at the station or bus stop. Nevertheless, earlier studies have only insufficiently considered these elements in itinerary planning. On the other hand, navigation is necessary in addition to itinerary creation. Particularly, recent navigation often considers dynamic information. During trips using public transportation, schedule changes are important dynamic information. For example, tourist arrive at bus stop earlier than planned. In such case, the waiting time will be longer than the waiting time included in the itinerary. In contrast, if a person is running behind schedule, a risk arises of missing bus. Nevertheless, earlier studies have only insufficiently considered these schedule changes. In this paper, we construct a tourism application that considers the waiting time to improve the tourism experience in rural areas. We define waiting time using static waiting time and dynamic waiting time. Static waiting time is waiting time that is included in the itinerary. Dynamic waiting time is the waiting time that is created by schedule changes during a trip. With this application, static waiting times is considered in the planning function. The dynamic waiting time is considered in the navigation function. To underscore the effectiveness of this application, experiments of the planning function and experiments of the navigation function is conducted in Tsuruoka City, Yamagata Prefecture. Based on the results, we confirmed that a tourist can readily plan a satisfactory itinerary using the planning function. Additionally, we confirmed that Navigation function can use waiting times effectively by suggesting additional tourist spots.

Structural Variants of Midnolin, a Genetic Risk Factor for Parkinson's Disease, in a Yamagata Cohort.

Parkinson's disease (PD) is a common neurodegenerative disease. We previously identified Midnolin (MIDN) to be a genetic risk factor for PD in both Yamagata (Japan) and British populations. However, the scale of our previous study was not sufficient to identify MIDN structural variants in the ascertained control of Yamagata Prefecture. We, therefore, reanalyzed MIDN variants in 3021 individuals from Yamagata Prefecture to compare with that in our previous British cohort study. MIDN copy number loss was only found in two cases (0.0662%), which was a lower frequency than that (1.64%) of the previously studied British cohort. Between the Yamagata and British groups, there was significant difference for rs3746106, located in the 5'-UTR of MIDN mRNA (p = 0.0003344, odds ratio 1.143), and for rs3746107, which corresponds to Ala34 (p < 2.2 × 10-16, odds ratio 5.89401). This study indicates that MIDN loss is relatively rare in the general Japanese population. Considering our previous studies that the frequency of MIDN loss is high among patients with PD (10.5 and 6.55% in Yamagata and Britain, respectively), the MIDN variants are much higher genetic risk factors for PD in a Japanese population than in a British population.

Changes in annual transcriptome dynamics of a clone of Japanese cedar (Cryptomeria japonica D. Don) planted under different climate conditions.

Environmental responses are critical for plant growth and survival under different climate conditions. To elucidate the underlying biological mechanisms of environmental responses in Japanese cedar (Cryptomeria japonica D. Don), the annual transcriptome dynamics of common clonal trees (Godai1) planted at three different climate sites (Yamagata, Ibaraki, and Kumamoto Prefectures) were analyzed using microarrays. Both principal component analysis (PCA) and hierarchical clustering of the microarray data indicated the transition to dormant transcriptome status occurred earlier and the transition to active growth status later in the colder region. Interestingly, PCA also indicated that the transcriptomes of trees grown under three different conditions were similar during the growth period (June to September), whereas the transcriptomes differed between sites during the dormant period (January to March). In between-site comparisons, analyses of the annual expression profiles of genes for sites 'Yamagata vs. Kumamoto', 'Yamagata vs. Ibaraki', and 'Ibaraki vs. Kumamoto' identified 1,473, 1,137, and 925 targets exhibiting significantly different expression patterns, respectively. The total of 2,505 targets that exhibited significantly different expression patterns in all three comparisons may play important roles in enabling cuttings to adapt to local environmental conditions. Partial least-squares regression analysis and Pearson correlation coefficient analysis revealed that air temperature and day length were the dominant factors controlling the expression levels of these targets. GO and Pfam enrichment analyses indicated that these targets include genes that may contribute to environmental adaptation, such as genes related to stress and abiotic stimulus responses. This study provided fundamental information regarding transcripts that may play an important role in adaptation to environmental conditions at different planting sites.