Last few decades, tamarind seed that is the food industrial waste was extracted their kernel to obtain xyloglucan for food, cosmetic, and medical applications. This study aimed to practically prepare and physically characterize the films containing xyloglucan (XG), bacterial cellulose (BC) and glycerol (GLY) for cosmetic applications. In the experiment, the films were prepared by varying (XG:GLY)-%BC ratio and using solution casting method. Preliminary, the films were observed the morphology. Subsequently, it was performed the adhesive test by using Texture Analyzer. From the preparation, it was found that the addition of BC provided the non-wrinkled films unlike the films that were prepared without BC addition. However, low transparent films were obtained from the addition of BC. From the adhesive test, the stickiness (SN), the adhesiveness (AN), and the cohesiveness (CN) were obtained simultaneously. The results revealed that the increasing of GLY and BC trendily increased SN and CN of the films, which (10:90)-9% films provided the highest SN (57.64 g) and AN (1.01 g.sec). However, (10:90)-9% films and (20:80)-8% provided insignificant difference of SN and AN. Meanwhile, (30:70)-7% films provided the lowest SN (10.77 g) and AN (0.21 g.sec). Moreover, the influence of GLY and BC on decreasing of CN was significantly found on (30:70)-7% films only. As the results, it could be concluded as the addition of BC into the films provided the non-wrinkled films, and the increasing of GLY provided the high adhesive films that suitable to apply on the skin for used as the transdermal patch. In the future works, the films will be added by the herbal extracts to provide biological activities such as anti-oxidation, anti-microbial, and anti-melanogenesis to the films.