X-ray Visibility Research Articles

Viscoelastic Properties of Fibrinogen Adsorbed to the Surface of Biomaterials Used in Blood-Contacting Medical Devices

The hemocompatibility of polymeric vascular implants is in part dependent on the propensity of fibrinogen to adsorb to the implant surface. Fibrinogen surface adsorption was measured in real time using a quartz crystal microbalance with dissipation monitoring (QCM-D). Six new, biodegradable tyrosine-derived polycarbonates were used as test surfaces. Stainless steel, poly(L-lactic acid), poly(D,L-lactide-co-glycolide), and poly(ethylene terephthalate) surfaces served as controls and provided a comparison of the test surfaces with those of commonly used biomaterials. Our study addressed the question regarding to which extent systematic variations in polymer structure can be used to optimize X-ray visibility and provide tunable degradation rates while generating protein-repellant surface properties that minimize fibrinogen adsorption. QCM-D revealed surface-dependent changes in fibrinogen layer thickness (2 to 37 nm), adsorbed wet mass (0.2 to 4.3 microg/cm2), and viscosity (0.001 to 0.005 kg/ms). While we did not find an overall correlation between surface air-water contact angle measurements and fibrinogen adsorption (R2 = 0.08), our data demonstrate that gradually increasing the poly(ethylene glycol) content within a subgroup of polymers having the same polymer backbone will lead to decreased fibrinogen adsorption. Within this subgroup of polymers, there was a strong correlation between decreasing air-water contact angles and decreasing fibrinogen adsorption (R2 = 0.95). We conclude that it is possible to minimize fibrinogen adsorption to tyrosine-derived polycarbonates while optimizing X-ray visibility and degradation rates. Some of the tyrosine-derived polycarbonates were identified as useful materials for the design of blood-contacting implants on the basis of their substantially lower levels of fibrinogen adsorption relative to the commonly used controls.

Analysis of RHESSI Flares Using aRadio Astronomical Technique

We have used Ramaty High Energy Solar Spectroscopic Imager (RHESSI) modulation profiles in the 25 – 300 keV range to construct high-fidelity visibilities of 25 flares having at least two components. These hard X-ray visibilities, which are mathematically identical to the visibilities of radio imaging, were input to software developed for mapping solar flares in the microwave domain using the Maximum Entropy Method (MEM). We compared and contrasted the MEM maps with Clean and Pixon maps made with RHESSI software. In particular, we assessed the reliability of the maps and their morphologies for future investigations of the symmetry of bipolar electron beaming in the sample set.

New acrylic microspheres for arterial embolization: Combining radiopacity for precise localization with immobilized thrombin to trigger local blood coagulation

Particles currently used in arterial embolization therapy have several disadvantages, most importantly their radiolucency. This means the radiologist cannot precisely asses the fate of embolization particles. Microspheres that combine two additional features have been designed. By incorporating an iodine containing monomer, radiopaque microspheres were obtained that display good visibility under standard X-ray conditions. Incorporation of methacrylic acid makes the surface of the spheres suitable for surface functionalization. Here, thrombin was covalently attached to the surface of the radiopaque microspheres. By induction of a thrombus, improved anchoring of the embolization spheres in the blood vessel can be obtained. The immobilized thrombin induced a biphasic response of the blood namely: (1) fast deposition of fibrin on the surface resulting in sphere aggregation and (2) additional thrombin generation in the surrounding blood and a subsequent local thrombus formation. These microspheres with both intrinsic X-ray visibility and a biofunctionalized surface can potentially improve embolization therapies.

Radio-Opaque and Surface-Functionalized Polymer Microparticles: Potentially Safer Biomaterials for Different Injection Therapies

Injectable polymer particles with a diameter in the range of 30-300 microm find applications as a biomaterial in different clinical fields, such as cosmetic surgery, reconstructive surgery, and urology. However, clinical effects tend to disappear after several months, either due to migration of the particles away from the injection site (caused by weak adherence with the surrounding soft tissues) or due to fibrosis (caused by excessive encapsulation of the particles by fibrous tissue). Little is known about the fate of injected microparticles, due to the fact that they are extremely difficult to trace in a noninvasive manner. Design, synthesis, and characterization of new polymeric microspheres with two additional features that can enhance safety and can help to overcome drawbacks of existing products are reported. First, the new microparticles feature clear radio-opacity (X-ray visibility) as they are prepared on the basis of a reactive methacrylic monomer that contains covalently bound iodine. Model experiments reveal that the level of X-ray contrast is sufficient for clinical monitoring; they can be visualized both during the injection and afterward. The particles feature excellent cytocompatibility in vitro and in vivo. Second, a method is explored to functionalize the surface of the particles, for example, through immobilization of collagen. Other extracellular matrix proteins can also be immobilized, and this provides a mechanism to control anchoring of the particles in soft tissue. The results are briefly discussed in the context of improved biomaterials, contemporary X-ray imaging, and control over biomaterial-soft tissue interactions in vivo.

Hard x-ray imaging for measuring laser absorption spatial profiles on the National Ignition Facility

Hard x-ray (“thin wall”) imaging will be employed on the National Ignition Facility (NIF) to spatially locate laser beam energy deposition regions on the hohlraum walls in indirect drive Inertial Confinement Fusion (ICF) experiments, relevant for ICF symmetry tuning. Based on time resolved imaging of the hard x ray emission of the laser spots, this method will be used to infer hohlraum wall motion due to x ray and laser ablation and any beam refraction caused by plasma density gradients. In optimizing this measurement, issues that have to be addressed are hard x-ray visibility during the entire ignition laser pulse with intensities ranging from 1013to1015W∕cm2, as well as simultaneous visibility of the inner and the outer laser drive cones. In this work we will compare the hard x-ray emission calculated by LASNEX and analytical modeling with thin wall imaging data recorded previously on Omega and during the first hohlraum experiments on NIF. Based on these calculations and comparisons the thin wall imaging will be optimized for ICF/NIF experiments.

Development of new injectable bulking agents: Biocompatibility of radiopaque polymeric microspheres studied in a mouse model

Radiopaque polymeric microspheres have a potential as new bulking agents for treatment of stress urinary incontinence (SUI). The advantage over existing bulking agents lies in their X-ray visibility in situ; other polymeric bulking agents (e.g., PTFE or silicone rubbers) are practically radiolucent (i.e., incapable of absorbing X-radiation). Radiopacity is useful in practice because of the high spatial accuracy of X-ray imaging. For instance, X-ray fluoroscopy can be used to assess possible migration of the bulking agent over time or to provide guidance in cases in which a second injection of a bulking agent is necessary (repeated treatment of SUI). Biocompatibility of injected radiopaque microspheres was investigated in vivo by using the mouse as a model. Microspheres were injected subcutaneously (9 animals) or intramuscularly (9 animals), and follow-up was 8 days or 3 months. X-ray fluoroscopy gave clear images of the microspheres as an ensemble, and it was found that no migration occurred during 3 months. Histopathology confirmed that all microspheres stayed close to the site of the injection. The microspheres appeared to be well tolerated; only a few giant cells, manifesting a mild inflammatory reaction, were encountered. At 3 months, capillary blood vessels were observed throughout the microsphere beds, and macrophages and fibroblast cells were seen in between the microspheres. This is encouraging with respect to the intended application, although it must be acknowledged that the data refer merely to a mouse model. Further experiments with larger, more representative models (rabbit and goat) are in progress.

X-ray opaque waveguide for dentistry.

The aim of this study is the design, realization, and examination of a new x-ray contrast hollow waveguide which will be suitable for x-ray diagnostics or treatment required in medicine, as dentistry, maxiollofacial surgery or oncology. With the growing usefulness of laser radiation therapeutic interventions, a strong demand for radiation delivery from the laser source to the interaction place has appeared. For more complicated surgery, especially for internal intervention, an x-ray is necessary. In such cases, as a minimum, the end of the delivery system must be x-ray contrasted to distinguish where the interaction of radiation with tissue would take place. Up to now, neither fiber, hollow waveguide, nor other delivery system have been x-ray opaque. A new type of hollow glass waveguide was designed, fabricated, and examined as a delivery system for an endodontic treatment. The system is composed from the cyclic olefin polymer coated silver hollow glass waveguide with special covering for x-ray visibility. The inner diameter of the waveguide was 320 microm, the outer diameter was 630 microm, and its length was 100 mm. After the delivery system was created, the hollow waveguide was checked under an x-ray machine and its opacity was measured. For actual treatment, an Er:YAG laser system generating a mid-infrared radiation was used. The root canals of 10 molars were treated endodontically, and the result of that treatment was compared with the common cleaning methods. During treatment, digital x-ray images were taken with conventional files, using this new designed hollow waveguide. The position of a metal instrument demonstrates the quality of opacity after conventional step-back technique. The hollow waveguide is visible in the left tooth canal. A bone density analysis shows no differences between a left (waveguide) and right (tooth) canal. It was demonstrated that no differences exist between x-ray opacity of the metal instrument used for endodontic treatment and a special hollow waveguide. The digitalized, three-dimensional image helps to detect a precise position of customary instrument or waveguide in root canal.

Radiopaque polymeric spinal cages: a prototype study

Back pain, originating from degeneration of intervertebral discs, is often alleviated by the insertion of one or more interbody fusion cages. The function of the cage is to restore the height between two adjacent vertebrae and to mediate osseous fusion. Most commercial cages consist of titanium or a titanium alloy, while polymeric cages, mostly consisting of polyether-etherketone (PEEK), are also in use. Titanium is known for its excellent biocompatibility. Titanium cages can be located easily with imaging techniques based on X-ray absorption (e.g. CT scans). However, they introduce artefacts in magnetic resonance (MR images). PEEK cages, on the other hand, do not show up in CT images. For this reason, small metallic markers are usually incorporated. The markers reveal the position of the cage, albeit indirectly. PEEK cages are clearly and integrally seen on MR images, as they are essential free of water. There are no artefacts or disturbances; this feature, as well as its strength, makes PEEK particularly attractive for the construction of cages. Here, we introduce new all-polymeric cages on the basis of an iodine-containing methacrylic copolymer (I-copolymer). This material has been prepared from methylmethacrylate and 2-[4-iodobenzoyl]-oxo-ethylmethacrylate. Copolymerisation of both monomers results in a high molecular weight material. Cytocompatibility experiments reveal that the material contains no toxic leachables and that cells can well adhere to and proliferate on the I-copolymer. Compression experiments at physiologically relevant strains disclose mechanical characteristics comparable to PEEK. The advantage of cages prepared from this I-copolymer over commercially available cages is that the present cage contains no metallic components, implying that it is compatible with MR imaging, and the presence of the iodine atoms ensures X-ray visibility.

Injectable polymeric microspheres with X-ray visibility. Preparation, properties, and potential utility as new traceable bulking agents.

The copolymer of methyl methacrylate (MMA) and 2-[2',3',5'-triiodobenzoyl]oxoethyl methacrylate (1), ratio 3:1 (mass:mass), was prepared via a free-radical polymerization in bulk. The copolymer (M(w) = 97.8 kD and M(n) = 41.5 kD) was dissolved in chloroform and subsequently transformed into beads with a diameter in the micrometer range, using a solvent evaporation technique. The resulting microbeads were characterized by different techniques, including NMR spectroscopy, differential scanning calorimetry, gel permeation chromatography, and scanning electron microscopy. The latter technique was used as the basis for statistical analysis of the bead size. Typically, an average diameter of 96 microm and a standard deviation of 21 microm were obtained. The beads were also subjected to some preliminary tests regarding cytotoxicity. The copolymer of MMA and 1 contains covalently bound iodine. Therefore, the material is intrinsically radiopaque, i.e., capable of absorbing X-radiation while no contrast additive is needed. Our interest in these microspheres stems primarily from their possible utility as injectable and afterward traceable (radiopaque) bulking agents, e.g., for use in urology for the treatment of female stress incontinence due to sphincter deficiency. As a first test into this direction, a sample of the microbeads was mixed with ethylene glycol, and the resulting suspension was studied with respect to injectability and radiopacity. The results suggest that the radiopaque microbeads may provide access to improved bulking agents. Further modification of the surface may be necessary in order to suppress the migratory aptitude of the radiopaque polymeric microspheres in vivo.

Neutron star retention and millisecond pulsar production in globular clusters

We investigate the conditions by which neutron star retention in globular clusters is favoured. We find that neutron stars formed in massive binaries are far more likely to be retained. Such binaries are likely to then evolve into contact before encountering other stars, possibly producing a single neutron star after a common envelope phase. A large fraction of the single neutron stars in globular clusters are then likely to exchange into binaries containing moderate-mass main-sequence stars, replacing the lower-mass components of the original systems. These binaries will become intermediate-mass X-ray binaries (IMXBs), once the moderate-mass star evolves off the main sequence, as mass is transferred on to the neutron star, possibly spinning it up in the process. Such systems may be responsible for the population of millisecond pulsars (MSPs) that has been observed in globular clusters. Additionally, the period of mass-transfer (and thus X-ray visibility) in the vast majority of such systems will have occurred 5–10 Gyr ago, thus explaining the observed relative paucity of X-ray binaries today, given the MSP population.

Initial experience with a co-radial bipolar pacing lead.

A new type of endocardial bipolar pacing lead has been designed to overcome the potential drawbacks of the conventional coaxial bipolar pacing lead. We prospectively evaluated the new co-radial bipolar pacing leads (Intermedics Thin-Line), which are thinner (5 Fr vs 6-8 Fr) than standard coaxial bipolar leads. X-ray visibility and lead handling were subjectively assessed (excellent, good, adequate, or poor) at implant; lead impedance, sensitivity threshold, and pacing threshold were measured at implant, then at 1, 3, 6, 12, and 18 months. The results were as follows: 103 patients (51 M; age 63.8 +/- 17.4 years) received 71 atrial (A) and 89 ventricular (V) leads. X-ray visibility was excellent in 59/103; good in 23/103; adequate in 11/103; and poor in 10/103. Overall handling was excellent in 56/71 A and 69/89 V; good in 11/71 A and 18/89 V; adequate in 3/71 A and 1/89 V; poor in 1/71 A and 1/89 V. There were two perioperative complications. At implant: impedance in A and V were 370.1 +/- 74.7 and 501.5 +/- 124.4 omega, sensing thresholds in A and V were 3.0 +/- 1.5 and 9.9 +/- 5.0 mV, pacing thresholds at 0.45 ms in A and V were 0.59 +/- 0.21 and 0.41 +/- 0.15 volt, respectively. At 1, 3, 6, 12, and 18 months of follow-up: no pacing lead related complications were reported; pacing lead characteristics remained outstanding and stable. This new lead appears to have significant clinical advantages over the conventional coaxial bipolar pacing lead. Long-term follow-up is required to confirm its reliability and chronic performance characteristics.

Studies on radio-opaque polymeric biomaterials with potential applications to endovascular prostheses

A new polymeric biomaterial, which uniquely combines radio-opacity (X-ray visibility) and low thrombogenicity, is described. First, preparation, purification, and identification of the essential monomeric building block, 2-[2′-iodobenzoyl]-ethyl methacrylate (3), are outlined. Second, ▪ the synthesis of the biomaterial, a terpolymer with composition MMA : HEMA : 3 = 65:15:20 (mole/ mole/mole) is described. Third, the physico-chemical characteristics of the polymer (e.g. NMR spectroscopy, thermal behaviour) are given. Fourth, the in vitro thrombogenicity of the material was characterized by means of recent test assay. The combined results reveal that the terpolymer is very suitable for prosthetic applications in the cardiovascular system. A new prototype of an endovascular stent, made from the terpolymer, is presented. Stents find clinical use in interventional cardiology, in conjunction with percutaneous transluminal coronary angioplasty (PTCA). It is put forward that the stent prototype presented herein has, at least in principle, some advantages over existing (metallic) stents; these advantages are primarily owing to the unique combination of X-ray visibility and haemocompatibility which is presently achieved.

Improvement of stenting therapy with a silicon carbide coated tantalum stent

State of the art cardiovascular stent materials are a compromise between bulk properties and surface related properties. As a consequence, deficiencies in both characteristics lead to serious limitations of stenting therapy. Beside a dissatisfying X-ray visibility of current stent materials, which hinders precise angiographic control of the stent during implantation, insufficient hemocompatibility causes subacute vessel occlusions despite stringent anticoagulant medication. Additionally, bleeding complications result which further limit the therapeutical success. Therefore it is essential to develop a new coronary stent with improved material properties for the bulk of the stent and its surface. This is realized by a hybrid concept. The stent is manufactured from tantalum, having a high inherent radio-opacity. The stent is coated with amorphous silicon carbide, optimized for hemocompatibility. An appropriate deposition technology to maximize coating adhesion was developed. Amorphous silicon carbide was investigated in vitro and in vivo to assess its suitability for coronary stents.

Improved Focusing for Extracorporeal Shock Wave Lithotripsy of Ureteral Calculi

Extracorporeal shock wave lithotripsy of ureteral calculi, which is usually performed with the patient in the prone position, may be difficult or even impossible under certain conditions because x-ray visibility is poor or the calculus is out of range of the shock wave focus. Treatment conditions can be improved by a simple change in the positioning of the patient, using the shock wave head from the contralateral side. This method is applicable for the common Siemens Lithostar device.

Improved Focusing for Extracorporeal Shock Wave Lithotripsy of Ureteral Calculi

Extracorporeal shock wave lithotripsy of ureteral calculi, which is usually performed with the patient in the prone position, may be difficult or even impossible under certain conditions because x-ray visibility is poor or the calculus is out of range of the shock wave focus. Treatment conditions can be improved by a simple change in the positioning of the patient, using the shock wave head from the contralateral side. This method is applicable for the common Siemens Lithostar device.

Studies on a new radiopaque polymeric biomaterial

A new radiopaque polymeric biomaterial has been synthesized. The material, which actually represents an entire family of analogous radiopaque materials, is composed of 2-( p-iodobenzoyl)ethyl methacrylate (compound 1, 21 mol%), methyl methacrylate (MMA, 60 mol%), and 2-hydroxyethyl methacrylate (HEMA, 19 mol%). The terpolymer was synthesized in a radical polymerization reaction at elevated temperature in N, N-dimethylformamide (DMF). The product was subjected to a set of physicochemical characterization techniques (gel permeation chromatography, 500 MHz 1H NMR in deuterated dimethylsulphoxide ( d 6-DMSO) solution, differential scanning calorimetry, dynamic water contact angle measurements), as well as to an in vitro thrombogenicity assay. Furthermore, scanning electron microscopy was used to study interactions of the material with blood platelets. The most important findings are: 1. (a) the material is a genuine polymer with excellent X-ray visibility, even in the form of thin (0.4 mm) drawn fibres. This was established under realistic conditions, 2. (b) The material exhibits low in vitro thrombogenicity, i.e. comparable to polyvinyl chloride, which is known as a passive material. These observations lead us to the suggestion that this type of radiopaque polymer holds promise with respect to application as a construction material for a new type of endovascular stent. This could be relevant in particular to stents to be used in conjunction with percutaneous transluminal coronary angioplasty (PTCA), also known as Dottering. Currently there is a clear trend away from metallic stents towards all-polymeric stents, since the latter have superior biocompatibility. Biocompatibility of the stent material is of vital importance with respect to the long-term performance of the stent, i.e. its ability to prevent the phenomenon of restenosis. Note that both stent material- vessel wall and stent material-blood interactions are crucial. Application of a radiopaque polymer as described herein should enable us to construct a new stent type with improved biocompatibility with respect to the metallic counterparts, combined with excellent X-ray visibility.

X-ray bright surges

We present evidence of X-ray emission from surges that are bright in Hα. These surges have many features common to flaring arches of Martin and Svestka (1988); the basic difference between the two is that in flaring arches cold and hot plasma are injected into clearly defined closed magnetic loops, while in the surges the injection goes into large-scale magnetic field structures of which the second footpoint is usually unknown. Because of the steep density gradient in such large-scale structures, the X-ray visibility of bright surges is limited to a few tens of seconds only. A series of repetitive surges, some of them bright and emitting X-rays, occurred on 8 July, 1980 from footpoints of two large-scale coronal structures, which might have been the legs of an enormous arch at least 600 Mm long.

The short-range-order of mechanically alloyed amorphous NiZr: A comparison of experiment and simulation

Amorphous Ni x Zr 1− x powders were prepared by mechanical alloying in the comparison range 0.2 ⩽ × ⩽0.7. Total atomic pair distribution functions G( r) and total radial distribution functions RDF( r) were derived from X-ray measurements. The atomic structure of the amorphous alloy was computer simulated by a modified dense random packing of hard spheres (DRPHS) which allows an adjustable overlap of unequal spheres. The calculated packings were relaxed by a Morse potential and the total atomic pair distribution functions were computed taking into account the different X-ray visibilities of Ni and Zr. There is good agreement between experimentally determined and calculated short-range-order in the whole homogeneity range of amorphous phase using next neighbour distances r NiNi = 2.48 A ̊ , r ZrZr = 3.10 A ̊ and r NiZr = 2.65 A ̊ .

A Thermal Catastrophe in a Resonantly Heated Coronal Loop

A theory for the thermal stability of hot coronal loops is presented, which is based on the resonant electrodynamic heating theory of Ionson (1982) and the evaporation/condensation scenario of Krall and Antiochos (1980). The theory predicts that gradual changes in the length of a loop or in its magnetic field strength can trigger catastrophic changes in the X-ray visibility of the loop, without the need for a change in the magnetic field topology.A natural explanation is thereby given for the observations of X-ray brightenings in loops and loop evacuations with coronal rain.

X-ray visibility of the dark adapted eye

May I refer to the observations of Dr. Cochrane Shanks (1978) and the subsequent correspondence? (Emery 1979; O'Connor 1979). It is of interest to note that Professor Silvanus Thomson in the inaugural presidential address to the Röntgen Society on November 5th 1897 described this phenomenon (Thomson 1897).