Immunochromatographic test strips (ITSs) have gained great practicability in point-of-care testing (POCT). Developing novel labels of ITSs with an additional signal to carry out quantitative analysis favors the development of sensitive POCT. Herein, tannic acid (TA) assisted stripping WS2 nanosheets (TA-WS2 NSs) based on colorimetric/photothermal ITS (TWITS) for clenbuterol (CLE) monitoring. After WS2 NSs loaded TA, it retained excellent photothermal performance and an intact surface structure for monoclonal antibody (mAb) labeling, at the same time, the stability of WS2 NSs and the activity of mAb were improved effectively. Satisfactory, the visual limit of detection (vLOD) of the photothermal signal was 0.3 ng/mL, which was enhanced 7-fold at least compared with traditional gold-based nanoparticles ITS (GNPs ITS). Importantly, this work can offer a new referenced method for other small molecule pollutants.