Shear behavior is one of the most important mechanical characteristics that contributes to the performance and appearance of woven fabrics. Because of anisotropy, shear properties of woven fabric are tested in various directions. This research is focused on the experimental study of shear properties of plain woven fabric when shear force acts on specimens that are cut at different angles to the direction of the weft. Tests were conducted on woven fabric specimens that were fastened in two parallel clamps of the tensile tester. Five cotton woven fabrics of different weft density and of the same warp density were used. The research results show a very high degree of correlation between shear force and its axial component for all directions of the cutting specimen, and likewise between the relative extension of the diagonal of the specimen and the vertical displacement of the specimen. The initial shear modulus of woven fabrics was determined experimentally and theoretically.