Wounds In Rabbits Research Articles

A rapid and robust organ repair polyacrylamide/alginate adhesive hydrogel mediated via interfacial adhesion-trigger molecules.

Adhesive hydrogels have been widely explored as tissue adhesives for wound sealing and repair. However, developing adhesive hydrogels with simple preparation techniques and strong adhesion to internal organs in a short time remains a challenge. In this study, we developed a strategy for robust and rapid tissue adhesion of internal organ sealing and repair by an interfacial adhesion-molecule triggered hydrogel system. In this system, polyphenol molecules act as adhesion-trigger reagents to achieve fast and strong adhesion of polyacrylamide/alginate hydrogels on the surface of wound tissue by rapidly forming abundant hydrogen bonds at the interface. The adhesion energy is significantly enhanced by 45 times under the mediation of polyphenol adhesion-trigger molecules, resulting in a robust (> 600Jm-2) tissue adhesion in just 30s. This interfacial adhesion system demonstrates good biocompatibility, strong sealing performance on multiple organs (porcine heart, lung, stomach, and intestine), and excellent repair properties in gastric perforation wounds of rabbits in vivo. Moreover, immunocytochemical and transcriptomic analyses reveal that this interfacial adhesion system significantly promotes vascular regeneration and inhibits inflammatory responses during wound repairing. The proposed hydrogel provides a facile strategy for rapid and robust tissue adhesion, and shows potential applications in organ sealing and repair.

Umbilical cord mesenchymal stem cell exosomes combined with collagen sponges contribute to neovascularization and wound healing in exposed bone wounds

BackgroundUnmanaged bone exposed wounds that don't receive proper treatment can potentially lead to additional complications. Human umbilical cord mesenchymal stem cell (HUC-MSC)-derived exosomes (EXO) have a significant effect in promoting wound healing. MethodsHUC-MSCs and HUC-MSC-derived EXOs were added to a CS to prepare HUC-MSC + CS and HUC-MSC EXO + CS complexes, respectively. The CS and prepared CS complexes were observed by scanning electron microscopy and applied to bone-exposed large-area skin wounds in rabbits. Then, wound tissues were collected and analyzed. Hematoxylin and eosin and Masson staining were performed to observe histopathological features in wound tissues. Immunohistochemistry and immunofluorescence were performed to observe neovascularization and neural fiber density. The expressions of growth factors (platelet-derived growth factor receptor β [PDGFR-β], vascular endothelial growth factor receptor 2 [VEGFR2], and epidermal growth factor receptor [EGFR]) in wound tissues were detected by western blotting. ResultsOn healing days 3, 7, 10, 14, and 16, compared with the control group, wounds in the CS group healed well. Coverage by collagen fiber was evident, angiogenic factor CD34 expression and neural fiber density increased, along with high EGFR, PDGFR-β, and VEGFR2 levels. Compared with the CS group, the HUC-MSC + CS and HUC-MSC EXO + CS groups had a better neovascularization and wound healing. In particular, the HUC-MSC EXO + CS group showed abundant collagen. ConclusionIn the treatment of bone-exposed wounds, HUC-MSCs and HUC-MSC-derived EXOs loaded in CS were more efficient than CS alone, contributing to neovascularization in wounds.

Bacteriophage-like Nanobiocatalysts with Spiky Topography and Dual-Atom Sites for Treating Drug-Resistant Bacteria.

Overuse of antibiotics leads to the proliferation of drug-resistant bacterial strains, worsening global morbidity, and mortality rates. Bioinspired nanomaterials present a promising avenue for developing nonantibiotic strategies against drug-resistant bacteria. Here, we engineer a bacteriophage-inspired artificial nanobiocatalyst via nonstoichiometric W18O49 that features a spiky topography and synergistic dual-atom sites for combating drug-resistant bacterial infection. Benefiting from the strong interaction within the synergistic Fe-O-Mo sites, the synthesized spiky artificial nanobiocatalyst exhibits superior reactive oxygen species (ROS)-catalytic activity, attributed to the regulated adsorption affinity between the reaction intermediates and catalytic sites. The experimental and theoretical investigations demonstrate that the bioinspired biocatalyst can effectively capture and kill bacteria through its spiky morphology and potent ROS-catalytic activity, which can enable a significant reduction in bacterial viability through downregulating genes associated with biosynthesis, cellular maintenance, and respiration. In vivo experiments demonstrate that the spiky artificial biocatalyst accelerates the reconstruction of drug-resistant bacteria-infected skin wounds in rabbits, exhibiting efficacy comparable to that of vancomycin. It is expected that this bioinspired study on spiky artificial nanobiocatalysts offers a straightforward path to facilitate the development of both bionic and nonantibiotic disinfection strategies.

Milk-Based Ointment Accelerates Wound Healing: A Comparative Study in Rabbits

Background: Wound healing is a multi-stage process that restores tissue integrity following injury. While chemical treatments like phenytoin aid in healing, traditional remedies, such as milk-based applications, remain underexplored. Milk, particularly its proteins, is known for promoting tissue repair and collagen synthesis. This study evaluated the wound-healing efficacy of a milk-based ointment (MO) on open skin wounds in rabbits. Methods: Male New Zealand white rabbits were randomly assigned to six groups, including negative controls, placebo (eucerin), and 1% phenytoin, alongside three MO groups (2%, 5%, 10% concentrations). The treatments were applied twice daily for 14 days. Wound healing was assessed through macroscopic analysis, tensile strength measurement, hydroxyproline levels, and histological examination. Results: The 5% MO-treated group exhibited the fastest wound healing, achieving full closure in 15 days, outperforming both controls and the phenytoin-treated group (17 days). Hydroxyproline levels and tensile strength were significantly higher in the 5% MO group, indicating enhanced collagen production and stronger tissue regeneration. Histological analysis further confirmed accelerated epithelialization and granulation in MO-treated groups. Conclusion: The study demonstrated that milk-based ointments, particularly at a 5% concentration, significantly enhance wound healing, suggesting their potential as cost-effective, biocompatible alternatives to conventional chemical treatments like phenytoin. Further research is needed to explore clinical applications in humans.

Comparative Efficacy of Manuka Honey and its Combination with Fusidic Acid in Surgically Inflicted Methicillin Resistance Staphylococcus aureus Infected Wounds in Rabbits

Comparative Efficacy of Manuka Honey and its Combination with Fusidic Acid in Surgically Inflicted Methicillin Resistance Staphylococcus aureus Infected Wounds in Rabbits

Effect of pomegranate peel with/without autologous bone marrow on healing of acute cutaneous wounds in alloxan-induced diabetic rabbits.

Healing of bum wounds is commonly associated with many complications. Every year various new repair materials are developed and experimentally used for treating burn wounds. Humans with diabetes mellitus usually suffer from chronic wound healing. Vascular, neuropathic, immune function, and biochemical abnormalities each contribute to the altered tissue repair. One underlying factor that accompanies all diabetic ulcerations is poor vascular flow, a circumstance that impedes proper wound healing. Numerous studies have highlighted the importance of adequate vascular sufficiency and vessel proliferation in tissue repair and the lack thereof in diabetic wound healing. Other studies have looked at whether disarrayed capillary remodeling and maturation of vessels might play a role in impaired diabetic wound healing. This investigation has been planned to report the influence of treatment with a mixture of both the powder of pomegranate peel (PP) accompanied with an autologous bone marrow (BM) on the cure of burn injuries in experimentally induced diabetic rabbits. Alloxan monohydrate has been applied to create diabetes in 50 rabbits. Then in each rabbit, two deep second-degree burn wounds were experimentally created. The animals were then divided randomly into 5 treatment sections: non-treatment controls (C1), treated with an available commercial powder for wound (C2), treatment with powder of PP, treatment with alone BM, and the final group treated with PP powder with bone marrow (PPBM). The speed of wound closure and the histopathological changes during healing were measured. The levels of the biomarkers of rabbit platelet-derived growth factor AA (PDGF-AA) and rabbit protease-activated receptor 1 (PAR-1) were measured on days 0, 4, 8, and 12. Wound healing was markedly more rapid in all the treatment groups versus the control non-treated group. Interestingly, a rapid wound cure was significantly observed in the PPBM group versus the other treatment ones. The histological assessment clarified a significant elevation in the fibroblast and collagen scores in the PPBM group versus the other sections. In addition, there were significant increases in the serum levels of the biomarkers PDGF-AA and PAR-1 among groups. Dependent on the results of current research, it can be concluded that both PP powder with BM PPBM significantly accelerate the healing process of burn wounds in experimentally induced diabetic rabbits.

Isolation of aerobic bacteria from abscesses and wounds in rabbits and antibiotic susceptibility testing of Staphylococcus spp. and Pseudomonas spp. isolates

BackgroundWound infections and abscesses in rabbits are a common problem in veterinary practice and their treatment can be challenging. Bacterial culture and antimicrobial susceptibility testing are important tools in these cases. While some studies are available on bacteria associated with wound infections and abscesses in rabbits, the numbers of animals included is generally limited. Monitoring of antibiotic resistance in clinical practice is also an important part of good antibiotic stewardship. Multiple antibiotic resistances are frequently encountered in Staphylococcus and Pseudomonas spp., two groups of bacteria commonly associated with abscesses in rabbits. MethodsThis retrospective study evaluated the results of aerobic bacterial cultures and antibiotic susceptibility tests in swabs from abscesses (A) (n=541) and from skin and wounds (SW) (n=308) submitted to a commercial diagnostic laboratory. Samples submitted between January 2017 and October 2020 were included in the study. Antimicrobial susceptibility testing for Staphylococcus and Pseudomonas spp. was evaluated and compared. ResultsIn A, 465 bacterial isolates including 35 different species were isolated while 394 bacterial isolates, including 35 different species, were found in SW. The most frequently isolated bacteria were Staphylococcus spp. (A = 21.7%, SW = 36.5%) followed by Pasteurella spp. in A (13.3%) and aerobic spore-forming bacteria in SW (11.2%). Pseudomonas spp. was the third most commonly isolated genus in both sample types (A = 11.6%, SW = 8.9%). Pseudomonas aeruginosa isolated from A were more often resistant to the tested antibiotics than other Pseudomonas species. These results support the need for bacterial culture and antibiotic susceptibility testing in small mammal practice.

Comparative evaluation of two different xenogenic acellular matrices on full-thickness skin wound healing.

The purpose of the study was to compare the healing potential of bubaline small intestinal matrix (bSIM) and fish swim bladder matrix (FSBM) on full-thickness skin wounds in rabbits. Four full-thickness skin wounds (each 20×20mm) were created on the dorsum of 18 rabbits that were divided into three groups based on treatment: untreated sham control (I), implanted with double layers of bSIM (II) and implanted with double layers of FSBM (III). Macroscopic, immunologic and histologic observations were made to evaluate wound healing. Gross healing progression in the bSIM and FSBM groups showed significantly (p<0.05) less wound contraction compared with the sham group. The IgG concentration in rabbit sera was significantly (p<0.05) lower in the FSBM group compared with the bSIM group by enzyme-linked immunosorbent assay. The stimulation index of peripheral blood lymphocytes was significantly (p<0.05) lower in the FSBM group compared with the bSIM group by the MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay. Implantation of FSBM resulted in improved re-epithelialisation, neovascularisation and fibroplasia. The FSBM is a more effective dermal substitute when compared with the bSIM for full-thickness skin wound repair in rabbit.

Use of Therapeutic RNAs to Accelerate Wound Healing in Diabetic Rabbit Wounds.

Introduction: Diabetes mellitus (DM) affects over 422 million people globally. Patients with DM are subject to a myriad of complications, of which diabetic foot ulcers (DFUs) are the most common with ∼25% chance of developing these wounds throughout their lifetime. Innovation: Currently there are no therapeutic RNAs approved for use in DFUs. Use of dressings containing novel layer-by-layer (LbL)-formulated therapeutic RNAs that inhibit PHD2 and miR-210 can significantly improve diabetic wound healing. These dressings provide sustained release of therapeutic RNAs to the wounds locally without systemic side effects. Clinical Problem Addressed: Diabetic foot wounds are difficult to heal and often result in significant patient morbidity and mortality. Materials and Methods: We used the diabetic neuroischemic rabbit model of impaired wound healing. Diabetes was induced in the rabbits with alloxan, and neuroischemia was induced by ligating the central neurovascular bundle of each ear. Four 6-mm full-thickness wounds were created on each ear. A LbL technique was used to conformally coat the wound dressings with chemically modified RNAs, including an antisense oligonucleotide (antimiR) targeting microRNA-210 (miR-210), an short synthetic hairpin RNA (sshRNA) targeting PHD2, or both. Results: Wound healing was improved by the antimiR-210 but not the PHD2-sshRNA. Specific knockdown of miR-210 in tissue as measured by RT-qPCR was ∼8 Ct greater than nonspecific controls, and this apparent level of knockdown (>99%) suggests that delivery to the tissue is highly efficient at the administered dose. Discussion: Healing of ischemic/neuropathic wounds in diabetic rabbits was accelerated upon inhibition of miR-210 by LbL delivery to the wound bed. miR-210 inhibition was achieved using a chemically modified antisense RNA.

Formulation and Test of Burns Healing Effects of Preparations Cream Extract Leaf Nangka Kuning (Vincetoxicum villosum (Blume) Kuntze)

V. villosum (Blume) Kuntze shows inhibition of the growth of Pseudomonas aeruginosa which is usually the cause of infection in burn wounds. The aim of this research was to formulate V. villosum (Blume) Kuntze leaf extract into a cream preparation and determine the time needed for V. villosum (Blume) Kuntze leaf extract cream to heal burn wounds in rabbits. This research was a type of experimental research. The research was conducted at the University of Bengkulu Laboratory in September 2022-August 2023. The equipment used was the Ohaus Starter 3100 brand pH meter and the NDJ-8S viscometer. The materials used are V. villosum (Blume) Kuntze leaf extract, mice, ethanol solvent. Oil-in-water (O/W) phase cream formulation with 3 variations of stearic acid and triethonolamine emulsifiers (15:4), (16:3) and (17:2). Then the three formulas were evaluated for their preparations which included organoleptic tests, homogeneity tests, pH tests, adhesion tests, spreadability tests, viscosity tests and descriptive stability tests. The activity test against burn wound infections used rabbits consisting of groups K1 (without treatment), K2 (given 0.2% hyaluronic acid cream), P1 (given 2% V. villosum (Blume) Kuntze leaf extract), and P2 (given 2% V. villosum (Blume) Kuntze leaf extract cream). Groups K2, P1 and P2 showed perfect wound healing on day 12. Meanwhile group K1 experienced incomplete wound healing until day 14. Based on the results of the study it can be concluded that the cream formulations I, II and III were declared stable at a temperature of 25º and the extract cream Kuntze V. villosum (Blume) Kuntze leaves showed complete wound healing in 12 days. The results of the Kruskal Wallis test obtained p value = 0.670 &gt; 0.05, which means there is no difference in the length of the healing process for all treatments.

Penyembuhan Luka Bakar Gel Enzim Bromelin Secara In Vivo

Bromelain is a proteolytic enzyme or natural protease found in tissues, including the stem, fruit, and leaves of pineapple (Ananas comosus var. comosus) and other plant species of the Bromeliaceae family. Bromelain is known as an efficient debdriding agent because it is beneficial in burn healing and tissue regeneration. This research aims to determine the effectiveness of bromelain enzyme for burns wounds in rabbits (Oryctolagus cuniculus). This research began with the optimization of carbopol 940 base preparations with variations in concentration, namely Formula 1 (F1) for 0,5%, Formula 2 (F2) for 1%, and Formula 3 (F3) for 1,5%. The evaluation of the gel formulation includes an organoleptic test, homogeneity test, pH test, viscosity test, adhesive force test, and spreadability test. The evaluation result indicates the base concentration in Formula 1 (F1) at 0,5% has met the requirements for a gel preparation. Formula 1 (F1) for 0,5, then combined with varying concentration of bromelain, namely Formula 1A (0,1%), Formula 1B (0,5%), and Formula 1C (1%). The gel formulation wa re-evaluated, including an organoleptic test, homogeneity test, pH test, viscosity test, adhesion test, spreadability test, irritation test, and in vivo test. In vivo effectiveness testing and irritation testing were conducted on five treatment groups, namely a positive control group (Bioplacenton®), Formula 1A (0,1%), Formula 1B (0,5%), Formula 1C (1%), and a group without treatment, then observed for 15 days. The observation data were processed using the One Way ANOVA test. Based on the results of the irritation test, Formula 1A (0,1%). Formula 1B (0,5%) and Formula 1C (1%) did not cause skin irritation. In the in vivo test, Formula 1C (1%) indicated the fastest recovery compared to other treatment groups, as evidenced by the decrease in wound diameter and visual observation, where the wound had started to close completely on day 15 of observation.

Effects of Laurus nobilis Leaf Extract on Healing of Experimentally Induced Wounds in Rabbits.

Wound or injury can be defined as partial or complete separation of the skin, while the healing of the wounds is defined as the sequences of processes by which the skin heals and returns to its normal appearance. This study aims to evaluate the efficacy of Laurus nobilis leaf extracts on the healing of open wounds in rabbits. Twenty-four healthy rabbits were used, divided randomly into control and treated groups, each consisting of 12 rabbits. The rabbits were caused to experience circular wound defects (2.5 cm) in diameter. All animals in the control (C) group were left without treatment while those in the Laurus nobilis leaf extract group were treated with drops of the aqueous extract of Laurus nobilis in a dose of 200 mg/kg per day for 20 days. The results of macroscopic observation revealed that all animals in both groups showed equitable viability and good appetite, no mortality occurred, and no signs of infection. In the group with the Laurus nobilis extract, we noticed a significant improvement in wound-healing activity and a reduction in the wound area at p ≤ 0.05 compared with the C group. Histopathological results at the end of the study show that the thick epidermal layer covers a mass of granulation with congested blood vessels and the dermis transforms into a denser layer gradually due to the improvements of the cellularity in the C group and also revealed a well-formed skin appearance, widespread of collagen, and fibrosis within the dermis with an appearance near the normal dermis in the group treated with Laurus nobilis leaf extract. In conclusion, the results confirmed that using Laurus nobilis methanolic extract solution in a daily dose of 200 mg/kg promotes healing of open wounds in rabbits.

Comparação entre as ondas contínua e pulsada do laser de baixa intensidade na cicatrização de enxertos cutâneos aplicados em feridas recém-criadas de coelhos (Oryctolagus cuniculus)

ABSTRACT: Skin grafting is a simple and very useful surgical technique for wound repair, especially where there is difficulty in performing direct synthesis or other reconstructive techniques. low-intensity laser (LIL) has already been used successfully in cases where the graft was applied to wounds shortly after its creation. However, LIL still lacks standardization due to conflicting studies on its results. Thus, this study compared the effectiveness of continuous wave LIL with pulsed wave LIL in stimulating the healing of skin grafts, applied to newly created wounds in rabbits. For this purpose, 31 rabbits were distributed into: control group (CG, n = 10), continuous wave laser group (GLC; n = 10) and pulsed wave laser group (GLP; n = 11). Postoperative management was similar between all groups, with the exception of laser application, as indicated by the group. The GLC and GLP groups showed similar evolution, with a satisfactory healing process of the graft, unlike the GC group. These results were maintained in the microscopic evaluation, as the groups treated with laser showed less inflammation, better integration with the receptor area, greater re-epithelialization and collagenization. There was no difference in vascular density between the treatment groups on immunohistochemical analysis. The newly created wound was not able to provide nutrition for the skin graft in rabbits, but LIL is an efficient adjuvant therapy in stimulating healing between the recipient area and the graft, promoting full integration. However; although, statistically there was no difference in the form of light emission, pulsed or continuous, patients who received continuous wave LIL showed superior microscopic evolution.

Exploring the Potential Anti-Inflammatory and Wound-Healing Proprieties of Cepaea hortensis Snail Mucin

Long before its popularization as a skincare ingredient, snail slime was reported to have a variety of health-promoting attributes. Its medicinal use involved the treatment of ailments, such as anthrax, hernias, stomach pain, chest pain, as well as tuberculosis. The main aim of this study was to investigate the anti-inflammatory and wound-healing properties of snail slime from Cepaea hortensis (Müller, 1774) (brown garden snail). The slime was extracted by a mild method, and the anti-inflammatory characteristics were determined by croton-oil-induced mouse-ear edema. The histological study showed that the snail slime exerts its action at the level of inflammatory mediators, explaining the drastic decrease of edema (59% in comparison to the 47% of indomethacin). The daily application of 50 µL (15 mg/kg) of snail slime on excision wounds in rabbits proved to induce an almost full tissue repair after 24 days of treatment (87.80–92.7% wound closure in width and length, respectively). Analysis of the slime’s safety aspect allowed affirming the nontoxicity of snail slime on both the skin and eyes.

Comparative Evaluation of Masson's Trichrome and Picrosirius Red Staining for Digital Collagen Quantification Using ImageJ in Rabbit Wound Healing Research

The therapeutic potential of Pluronic F127 (PF127) hydrogel loaded with adipose-derived stromal vascular fraction (AdSVF), mesenchymal stem cells (AdMSC), and conditioned media (AdMSC-CM) for repairing full-thickness skin wounds was evaluated using a rabbit model. The rabbits were randomly divided into eight groups with six animals each and treatment was given as per the predetermined protocol (3 doses at one-week interval): Group A (Control), Group B (AdSVF), Group C (AdMSC), Group D (AdMSC-CM), Group E (PF127), Group F (AdSVF + PF127), Group G (AdMSC + PF127), and Group H (AdMSC-CM + PF127). Skin tissue samples were collected from the healing wounds on day 28 for staining and collagen quantification. Collagen density (Area %) was quantified using tissue sections stained with Masson's Trichrome (MT) and Picrosirius Red (PSR) stain using the Colour Deconvolution plugin of ImageJ and RGB stack method, respectively. These techniques function based on separating different colour channels in the stained tissue sections to isolate the collagen fibers and then quantifying them through thresholding and image analysis. Across the treatment groups, both staining methods generally showed a trend of increased collagen density compared to the control group. For most groups, PSR staining consistently indicated slightly lower collagen densities than MT staining. However, the overall trends were similar in both staining. The comparison between PSR and MT staining methods revealed that both techniques effectively assess collagen density in healing wounds. However, there were subtle differences in the absolute values obtained, with PSR staining tending to yield slightly lower collagen density measurements than MT. These differences can be attributed to the distinct mechanisms of these staining methods. Therefore, both staining methods can digitally quantify collagen density in wound healing research.

The Impact of Aloe Vera Gel on Healing of Surgically Made Maxillary Mucosal Wounds in Rabbits

Objective: The purpose of present study is to evaluate the effect of Aloe Vera gel on granulation tissue formation and angiogenesis and re-epithelialization stages of experimental healing process of maxillary oral mucosa in rabbits. Materials and methods: The current experimental study was carried out on twenty albino rabbits which were randomly selected and inhabited under same conditions involving ventilation, temperature and diet. Each rabbit was anesthetized separately and undergone surgical procedure in both sides of upper alveolar bone included longitudinal oral mucosal incision followed by circular bone defect creation, then placement of tiny gel foam piece and Aloe Vera gel in left side, while right side bone hole was l eft empty to act as control. According to euthanization time, rabbits were divided into five groups and sacrificed at the 3rd, 7th, 14th,28th and 42 days post-wounding, histological and histomorphometrical assessment included evaluation of am ount of granulation tissue formed and angiogenesis and re-epithelialization. Results: Histological results revealed a statistically significant difference in amount of granulation tissue and neo-vascularization formed and re-epithelialization between control and Aloe Ver a groups at certain time intervals within total period of experiment with favor to Aloe Vera group. Conclusion: The us e of Aloe Vera gel exhibits a beneficial effect by increasing granulation tissue formation and subsequent angi ogenesis and enhancement of re-epithelialization in healing process.

Injectable polyisocyanide hydrogel as healing supplement for connective tissue regeneration in an abdominal wound model

In pelvic organ prolapse (POP) patients, the uterus, bladder and/or rectum descends into vagina due to weakened support tissues. High recurrence rates after POP surgery suggest an urgent need for improved surgical outcomes. Our aim is to promote connective tissue healing that results in stimulated tissue support functions by surgically applying a hydrogel functionalized with biological cues. We used known vaginal wound healing promoting factors (basic fibroblast growth factor, β-estradiol, adipose-derived stem cells) in the biomimetic and injectable polyisocyanide (PIC) hydrogel, which in itself induces regenerative vaginal fibroblast behavior. The regenerative capacity of injected PIC hydrogel, and the additional pro-regenerative effects of these bioactive factors was evaluated in abdominal wounds in rabbits. Assessment of connective tissue healing (tensile testing, histology, immunohistochemistry) revealed that injection with all PIC formulations resulted in a statistically significant stiffness and collagen increase over time, in contrast to sham. Histological evaluation indicated new tissue growth with moderate to mild immune activity at the hydrogel – tissue interface. The results suggest that PIC injection in an abdominal wound improves healing towards regaining load-bearing capacity, which encourages us to investigate application of the hydrogel in a more translational vaginal model for POP surgery in sheep.

Design and fabrication of gelatin-based hydrogel loaded with modified amniotic extracellular matrix for enhanced wound healing

Trauma can damage the structural integrity of skin leading to its function being affected. There is an urgent clinical need for innovative therapeutic wound dressings. However, several challenges persist despite the current demands. The development and application of functional dressings offer a novel approach to address skin and subcutaneous soft tissue defects. Amniotic membrane as an ideal biological multifunctional material covering wound surface has been reported in clinic. However, current clinical applications of amniotic membrane still have limitations, such as thinness and mechanically weak. In this paper, we employed decellularized human amniotic membrane (dHAM) as a bioactive extracellular matrix (ECM) and modified it through methacrylate (MA) grafting for engineering purposes, resulting in the photosensitive dECMMA. Subsequently, we utilized a photosensitizer to achieve photopolymerization of dECMMA with GelMA hydrogel, successfully creating a novel composite hydrogel termed dECMMA/GelMA. This composite hydrogel not only inherits the favorable physicochemical properties of hydrogels but also maintains comparable levels of bioactivity to dHAM itself, supporting cell proliferation, migration, angiogenesis, and retaining significant anti-inflammatory capacity.Additionally, we evaluated the reparative effect of the designed dECMMA/GelMA composite hydrogel on rabbit wound defects. We demonstrated that the dECMMA/GelMA promoted wound healing and re-epithelization. These findings highlight the substantial benefits and therapeutic potential of the dECMMA/GelMA composite hydrogel as a practical solution for clinical applications in the treatment of soft tissue damage. Furthermore, this research provides a new strategy for designing and manufacturing bioactive dressings with exceptional clinical efficacy in the future.

Uji Efektivitas Sediaan Salep Ekstrak Daun Kenikir (Cosmos Caudatus Kunth) Terhadap Penyembuhan Luka Sayat Pada Kelinci Jantan New Zealand White

Kenikir Leaf Extract (Cosmos Caudatus Kunth) is a medicinal plant that can be used to heal cut wounds. This research aims to determine the compound content in kenikir leaves, determine the physical quality and stability of kenikir leaf extract ointment preparations, and determine the effective concentration for healing cuts on the backs of male New Zealand White rabbits. This type of research is experimental, making ointment formulations using kenikir leaf extract made in 3 concentrations, namely 10%, 15% and 20%. In ointment testing, organoleptic tests, homogeneity tests, spreadability tests, adhesion tests, pH and viscosity tests are carried out. Testing the effectiveness of wound healing using 5 groups, namely Betadine ointment (+), ointment base (-), kenikir leaf extract ointment 10%, 15% and 20% on 5 rabbits with a wound length of 1.5 cm was carried out until the 3rd day. 7. The data was processed statistically using One Way ANOVA (Analysis of Variant) and continued with the LSD (Least Significant Difference) test. The results of this research show that kenikir leaf extract ointment 10%, 15% and 20% produces an ointment that meets the requirements of the organoleptic test, homogeneity test, spreadability test, adhesiveness test and pH test, as well as providing healing effects on cuts and wounds in rabbits and The most effective healing power is 20% kenikir leaf extract ointment.

Formulasi Sediaan Gel Ekstrak Daun Alpukat (Persea americana Mill.) Sebagai Terapi Pengobatan Luka Bakar Terhadap Kelinci New Zeland White

Researched on burn wounds healing activity of extract of avocado leaft (Persea americana Mill.) in gel preparation forms were tested on rabbits New Zeland White. This study aimed to determine variations effect of concentration in gel extract of avocado leaft for the treatment of burn wounds in rabbits which had been wounded using hot metal. In this study, avocado leaft extract was obtained through maceration method and formulated into gel preparations with concentration of 1%, 2% and 5%. Gel base was used as a negativ control, namely carbomer 940 and Bioplacenton® gel was used as a positive control, applied to burns on the back of New Zeland White rabbits with a diameter of 1,5 cm. The avocado leaft extract gel activity test was carried out on 3 rabbit, each formulation was replicated 3 times and wound diameter measurements were taken on each day. Data on the percentage of burn wound healing was statistically analyzed using One Way ANOVA method. The result of the data analysis at a concentration of 5% obtained a sig value &lt;0,05. Based on One Way ANOVA analysis of the five treatments, the most effective was avocado leaft extract gel with a concentration of 5%.