Wounds In Wistar Rats Research Articles

Comparison Of The Effectiveness Of Grape Seed And Onion Extract In Healing Cut Wounds In Wistar Rats

In healing cut wounds, generally many use drugs with chemicals, the use of chemicals in wound healing has a lot of negative impacts, therefore it is necessary to conduct further research on the benefits of natural ingredients such as plants to treat cut wounds, some of the advantages of using medicinal plants include relatively safer, easy to obtain, cheap, does not cause resistance, and relatively harmless to the surrounding environment or the mixture between the plants, which has been used for treatment for generations. One way to treat cut wounds is to use herbal plants because they are cheaper, easier to get, and have low side effects. Compounds that play a role in the healing process of cut wounds include flavonoids, saponins, tannins, polyphenols, and essential oils. One of the plants that has health benefits is grapes. The purpose of this study is to find out the comparison of the effectiveness of grape seeds and onion extract in healing cut wounds in wistar rats. The type of research used in this study is experimental research with a true experimental method with a post-test only control group design pattern. The sample to be used in this study is 24 rats divided into 4 groups so that each group contains 6 rats. Data analysis uses the One-Way ANOVA test. The results showed that shallot gel extract had a significant effect on the percentage of reduction in incision length.

Effects of Fitoscar™ (dry extract of Stryphnodendron adstringens 50%) on the healing of acute wounds in Wistar rats

Several studies show the potential of Stryphnodendron adstringens (Mart.) Coville bark preparations in wound healing. A medicine called Fitoscar™ is based on this herbal medicine, which is commercially available and facilitates its acquisition by the patient. However, studies with Fitoscar™ are necessary to determine its effects during the healing processes. The aim of this study was to evaluate the effects of Fitoscar™ on different wound healing events induced in Wistar rats. Excisional wounds were experimentally induced in Wistar rats, divided into two groups: control: wound cleaning with warm 0.9% saline solution (39° to 40°C) and treated with Fitoscar™, subdivided into 04 subgroups, corresponding to 3, 7, 14 and 21 days of experimental period. Morphometric, macroscopic and microscopic analyzes were performed. Fitoscar™ influenced fibroblast migration and stimulated angiogenesis at days 3 and 7 respectively. While there was greater intensity of miofibroblasts at day 14. At 21 days, Fitoscar™ induced more crust and hypergranulation. While Fitoscar™ group presented lower percentage of wound closure on days 7 and 14, however all wounds healed completely in a similar time, 21 days. Fitoscar™ may favor wound healing, leading to fibroblast migration and angiogenesis during the inflammatory and proliferative phases in acute experimental wounds.

Mupirocin/hydroxyapatite composite suspended in transcutol P-spiked hydrogel: In vitro characterization and in vivo wound healing assessment

Objectives: Natural hydroxyapatite (HAP) has been utilized as a drug carrier owing to its excellent bioactivity and biocompatibility. This study is aimed at formulating mupirocin/hydroxyapatite composite suspended in hydrogel. The appropriate quantity of the penetration enhancer (Transcutol-P®) was also investigated. Materials and Methods: The HAP was isolated from bovine bone by hydrothermal treatment, calcined at 1000oC and held for 2 hours in an electric furnace to remove the organic contents. The bones were milled, sifted using 150 µm mesh sieve and characterized. Olive oil, which contains oleic acid, was utilized as a natural capping agent to prevent agglomeration of the particles in the formulation. The quantity of Transcutol-P® was varied with mupirocin used as the active pharmaceutical ingredient for the management of acute wound in Wistar rats. In this animal study, the wound closure rate was evaluated. Results: The formulation with the 0.6%v/v, of Transcutol-P® gave the best wound closure rate of 30.05 mm2/day. The in-vitro study showed that the formulation containing 0.6%v/v Transcutol-P® released 63.9% of the drug after 75 minutes while 42.4% was released at the same time interval when the concentration of the penetration enhancer was increased to 1.2%v/v. The mupirocin-encapsulated HAP hydrogel composite showed high resistance against staphylococcus saprophyticus with inhibition zone of 37.3 mm. Conclusion: The mupirocin encapsulated in HAP allows for sustained release of the antibiotic and thus serves as a veritable drug carrier suitable for wound healing applications. Transcutol-P® (0.6%v/v) is effective in facilitating drug release, which is reflected in the increased wound closure rate in Wistar rats.

Healing Effect of Wistar Rat Incision Wounds on Administration of Microemulsion Combination of Green and Red Betel Leaf Extracts

One natural ingredient that has the potential to heal wounds is betel leaf. This study aimed to analyze the healing effect of Incision wounds in Wistar rats when administering a microemulsion combination of green and red betel leaf extracts. This research begins with sample collection, drying process, and extraction. The extract was then formulated into emulgel as a microemulsion in three formulations. These three formulas, along with a positive control and a negative control, were tested on each Wistar rat incision wound. The results of the research showed that emulgel formula 3, which was tested on mice, had the best ability to heal wounds for an average of 2.5 days and kept the mice's body temperature normal; 36.6oC. Formula 3 contains 5% South Kalimantan red betel, 5% South Kalimantan green betel, 5% Central Kalimantan red betel, and 5% Central Kalimantan green betel. This proves that the combination of formula 3 can heal wounds and body temperature in experimental mice. Further research is needed to find the best composition that produces faster, more complete wound healing effects and safely impacts experimental animals.

Healing of Skin Wounds in Rats Using Creams Based on Symphytum Officinale Extract.

Rosmarinic acid is a well-known natural antioxidant and anti-inflammatory compound, and it is one of the polyphenolic compounds found in comfrey plants. Comfrey root also contains allantoin, which helps with new skin regeneration. This study aimed to investigate the healing and skin regeneration process of skin wounds in Wistar rats using creams based on comfrey extract and to correlate the results with active compounds in the extract. The obtained results showed that comfrey root is rich in bioactive compounds, including allantoin, salvianolic acid, and rosmarinic acid, which are known for their great free radical scavenging activity, and the high antioxidant activity of the extract may be mainly due to these compounds. The obtained extract has an antimicrobial effect on Staphylococcus aureus (1530.76/382.69), Escherichia coli (6123.01/6123.01), and Pseudomonas aeruginosa (6123.01/6123.01). The macroscopic evaluation and the histological analysis of the skin defects 14 days after the intervention showed faster healing and complete healing in the skin excisions treated with oil-in-water cream with 20% extract of comfrey as the active ingredient.

Effect of platelet-rich plasma plus stromal vascular fraction on epithelialization and collagenization of rectoanal wounds in Wistar rats

Effect of platelet-rich plasma plus stromal vascular fraction on epithelialization and collagenization of rectoanal wounds in Wistar rats

Reparative and Inflammatory Changes in Wounds in Animals with Different Resistance to Hypoxia

Individual resistance to hypoxia largely determines the severity of inflammatory processes and, probably, can affect the regeneration of the skin wound.The aim was to study reparative and inflammatory changes in the wound in Wistar rats with different resistance to hypoxia.Material and methods. The study was performed on mature male Wistar rats weighing 300–350 g. Before the experiment, the resistance of animals to hypoxia was determined. Rats were classified as highly resistant to hypoxia (HR), in which the time to take a lateral position in the pressure chamber “at a height” of 11,500 m was more than 360 s (n=5), to low-resistant (LR) – less than 40 s (n=5). A full-thickness wound with a diameter of 16 mm was applied to the animals in the interscapular zone of the back. On the 7th day after the infliction of the wound, a histological and morphometric study was performed. The proliferative activity of cells in the granulation tissue of the wound was determined immunohistochemically using the Ki-67 marker. Real-time PCR was used to evaluate the level of expression of the Hif-1α, Nf-κB, and Vegf genes.Results. It was shown that in rats with high resistance to hypoxia, the total number of cells and, among them, the number of inflammatory cells was higher than in rats with low resistance to hypoxia. However, the percentage of inflammatory cells in the compared groups did not differ significantly. Cell proliferative activity, as measured by the number of Ki-67 positive cells, was higher in rats with high resistance to hypoxia. In the granulation tissue of highly resistant to hypoxia rats, the expression of the Vegf gene was statistically significantly higher than in low-resistant animals, while there were no statistically significant differences in the level of expression of the Hif-1α, Nf-κB genes.Conclusion. In rats with high resistance to hypoxia, on the 7th day of healing of a full-thickness skin wound, reparative processes in it are more pronounced. In the horizontal layer of fibroblasts, there are more cells, among them inflammatory ones - histiocytes and macrophages, which is combined with a higher number of proliferating Ki-67 positive cells and the expression level of vascular endothelial growth factor (Vegf).

Bigel formulations of St. John’s wort extract in wound healing: toxicological aspects

This study aimed to investigate the toxicological profile of hyperforin (HP) in silico and to assess it in vivo after topical application of an HP-rich St. John’s wort (SJW) extract. The former analysis predicted low toxicity because of HP’s inability to bind DNA or proteins, but structural alerts for skin irritation/corrosion, carcinogenicity, and mutagenicity were found. Animal studies involved the treatment of excision wounds in Wistar rats with poloxamer 407/borage oil formulations (bigels; Bs) containing HP-rich SJW extract previously developed by us. The effects of semisolids comprising ‘free’ extract (B/SJW) or extract loaded in nanostructured lipid carriers (B/NLC-SJW) were compared to positive (commercial herbal product) and negative (untreated) controls after 2-, 7-, 14-, and 21-day applications. Malondialdehyde (MDA) and ABTS assays evaluated the degree of oxidative stress—treatment with bigels did not affect MDA favorably but led to an increased radical-cation scavenging capacity (compared to controls). Gamma-glutamyl transferase (GGT), aspartate aminotransferase (ASAT), alanine aminotransferase (ALAT), and lactate dehydrogenase (LDH) enzyme levels were measured as indicators for liver/tissue damage. Treatment with both B/SJW and B/NLC-SJW for 21 days resulted in lower GGT and ASAT levels than those in controls. Two-day application of the biphasic semisolids contributed to normalized ALAT levels (lower than in both negative and positive controls), and the same trends were observed in LDH levels after a 7-day treatment. The promising results obtained after the B/NLC-SJW application suggest that this drug delivery system may not only preserve HP in SJW extract effectively but also ‘expose’ its cyto-/hepatoprotective potential.

The Effectiveness of Sungkai Leaf (Peronema canescens Jack.) Extract Gel on the Collagen Density of Incision Wounds in Vivo

An incision is an open wound resulting in skin injury and underlying tissues. The wound-healing process involves a proliferative phase that produces collagen. Sungkai leaves (Peronema canescens Jack.) can be used as an alternative therapy for wound healing because it contains flavonoids, tannins, and saponins that stimulate fibroblasts to synthesize collagen. This study aimed to determine the effectiveness of sungkai leaf extract gel in healing incision wounds in vivo based on collagen density scoring. Twenty-five Wistar rats (Rattus norvegicus) were randomly divided into five groups, the negative control group (K1) was given a gelling agent CMC 1%, the positive control group (K2) was given bioplacenton gel 1%, the treatment group (K3, K4, K5) was given a gel sungkai leaf extract concentration of 5, 10, and 15. The treatment is given topically at the incision site of the incision site of 0.1 g once a day for 14 days. The rats were euthanized on the fifteenth day, and the incision area was excised for histopathological preparation. The data obtained were analyzed using Kruskal-Wallis with SPSS 26. The test results showed p=0.010, meaning there is a significant difference (p<0.05) between the entire treatment. Based on these results, the K4 group (Sungkai leaf extract gel concentration of 10%) showed the highest collagen density scoring results compared to other treatments. This study concludes that sungkai leaves effectively heal incision wounds in Wistar rats based on collagen density scoring parameter.

Mikania micrantha extract enhances cutaneous wound healing activity through the activation of FAK/Akt/mTOR cell signaling pathway

Mikania micrantha (MM) has been traditionally used for various health benefits, including mental health, anti-inflammatory, wound dressing, and healing of sores. However, the molecular mechanisms and dose required for the wound healing activity of MM have yet to be reported. Therefore, a study was conducted to evaluate the wound healing potential of a cold methanolic extract of MM through in vitro and in vivo studies. Human dermal fibroblast adult (HDFa) cells were treated with 0 (control), 75 ng/ml, 125 ng/ml, 250 ng/ml, and 500 ng/ml of MMmethanolic extract (MME) for 24 h. MME at 75 ng/ml has significantly (p˂0.05) promoted HDFa cell proliferation and migration. Further, MME has also been shown to enhance the invasiveness of human umbilical vascular endothelial cells (HUVECs), indicating the neovasculature for wound healing. The tube formation assay demonstrated a significant (p<0.05) increase in the angiogenic effect of the MME starting at a concentration of 75 ng/ml as compared to the control. Treatment of excision wounds in Wistar rats with 5% and 10% MME ointment significantly enhanced wound contraction compared to control animals. Incision wounds in rats treated with 5% and 10% MME showed a significant (p<0.01) increase in tensile strength compared to control. HDFa cells, and granulation tissue collected on day 14 post-wounding, revealed the modulation of the FAK/Akt/mTOR cell signaling pathway during the enhancement of wound healing. The results of gel zymography showed increased activity of MMP-2 and MMP-9 in the HDFa cells after treatment with the extract. It is concluded that MMEcan potentially accelerate cutaneous wound healing.

Topical delivery of mupirocin calcium nanostructured lipid carriers using a full-thickness excision wound healing model

Treatment of contaminated wounds represents a significant challenge in healthcare and there is a need to develop approaches maximising skin retention to maintain therapeutic concentrations of anti-infectives at the wound site. The objective of the present study was to develop and evaluate mupirocin calcium nanolipid emulgels to enhance wound healing performance and patient acceptability. Nanostructured lipid carriers (NLCs) of mupirocin calcium were prepared by the phase inversion temperature method using Precirol ATO 5 (Gattefosse, India) and oleic acid as lipids and Kolliphor RH 40 (BASF, India) as surfactant and further incorporated into a gel base for topical delivery. The particle size, polydispersity index and zeta potential of mupirocin NLCs were found to be 128.8±1.25nm, 0.283±0.003 and -24.2±0.56mV, respectively. In vitro release studies from developed emulgel showed sustained drug release over 24 hours. Ex vivo drug permeation studies through excised rat abdominal skin showed better skin permeation (1712.38±15. 57μg/cm2) from developed emulgel compared to marketed ointment (827.92±21.42μg/cm2) after 8 hours, which was in agreement with in vitro antibacterial activity. Studies on Wistar rats indicated the nonirritant potential of developed emulgels. Further, mupirocin emulgels showed improved efficacy in percent wound contraction of acute contaminated open wounds in Wistar rats using a full-thickness excision wound healing model. The emulgels of mupirocin calcium NLCs appear to be effective in the treatment of contaminated wounds due to increased skin deposition and sustained release, thereby enhancing the wound healing potential of existing molecules.

Synergic effect of bone marrow derived mesenchymal stem cells and differentiated keratinocytes-like cells with a novel cellulose and collagen nanoscaffold on wound healing in rats

Profound wounds could lead to massive infection or organ malfunction. The natural physiological process might fail to regenerate the skin flawlessly. On the contrary, conventional therapies have numerous drawbacks. Tissue engineering could offer a substitute therapy for wound regeneration. During this study, a pre-synthesized novel nano scaffold made of nano cellulose, collagen type I, and Carboxymethyl diethyl amino ethyl cellulose was used in combination with cell therapy to observe their synergic effect on wound healing in rats. Bone marrow-derived mesenchymal stem cells were differentiated into keratinocytes-like cells using EGF, BMP4, and FGF7. Differentiation was confirmed with real-time PCR and Immunocytochemistry. The scaffold alone and in combination with mesenchymal stem cells and differentiated cells were used to dress induced wounds in Wistar rats. The results showed the scaffold alone and in combination with cell therapy could assist in wound healing. The most effective treatment was the combination of the scaffold and differentiated cells.

PLGA/Gelatin-based electrospun nanofiber scaffold encapsulating antibacterial and antioxidant molecules for accelerated tissue regeneration

A full-thickness or hard-to-heal wound pose a significant challenge in clinical feilds. These wounds are very prone to microbial infection, lead to an elevated level of reactive oxygen species, and require a skin regeneration product for rapid healing. Considering these issues, we developed ciprofloxacin hydrochloride and quercetin encapsulated PLGA/Gelatin based electrospun nanofibers for wound healing. Morphology and diameter of nanofibers were determined by SEM. FT-IR and powder-XRD was used to determine any possible physic-chemical interaction and change in the crystalline property of drugs, respectively. In-vitro swelling ratio and weight loss was measured to simulate in-vivo degradation pattern of scaffold. High drugs entrapment with a bi-phasic sustained release profile was observed in phosphate buffer (pH =7.4). DPPH scavenging potential and antibacterial activity against S. aureus on agar plate were utilized to evaluate antioxidant and antibacterial properties. The biocompatibility of developed nanofiber was assessed by MTT assay using 3T6-Swiss albino fibroblast and hemocompatibility assay using harvested erythrocytes from human blood. After excising full-thickness wounds in Wistar rats, the healing efficacies of nanofiber were evaluated, this showed accelerated healing with complete re-epithelialization within 16 days. Hematoxylin & eosin staining and hydroxyproline assay confirmed the in-vivo wound healing observation. These findings support the use of ciprofloxacin hydrochloride and quercetin-loaded PLGA/Gelatin based nanofiber as a dressing material for full-thickness wounds.

Safety and efficacy of Lucilia cuprina maggots on treating an induced infected wound in Wistar rats

Infection is one of the main complications that hinder wound healing. Currently, antibiotic-resistant bacteria, such as Methicilin-resistant Staphylococcus aureus (MRSA), are a concern worldwide for both humans and animals. Maggot therapy is re-emerging as an alternative to intractable wounds and may be an option to the traditional antibiotic treatment. Although the species of choice is Lucilia sericata, reports of clinical use have led us to evaluate the efficacy and safety of using Lucilia cuprina larvae on induced infected wounds in Wistar rats. In short, 32 male Wistar rats were divided into 4 groups: Group I - saline solution treated; Group II - antibiotic-treated; Group III - treated with larval debridement, and Group IV - without wound and treatment. Skin wounds were induced in groups I, II and III. All treatments were performed once and held for 48 h. Clinical, microbiological, histopathological, hematological, and biochemical analyses were done. Significant wound area contraction was found (>95%) in group III on day 9 compared to day 15 in group II. Complete elimination (0.0 ± 0.0 CFU/mL) of bioburden was achieved after the second treatment (day 6) in both the II and III groups, compared to an increase in Group I (6.51 ± 0.37 CFU/mL). A cleaner wound was also observed in the histopathological evaluation of group III, with adequate collagen formation and re-epithelialization on day 15. Furthermore, larvae increased blood platelet levels after the first treatment. L. cuprina larvae have proven safe and effective in accelerating wound treatment and eliminating MRSA.

In vivo Evaluation of the Hemostatic and Cicatrising Activities of a Gel Based on Vernonia Conferta Benth. On Post-extraction Wounds in Wistar Rats

Introduction: Despite the progress in oral surgical techniques, the occurrence of post operative haemorrhage can lead to delayed healing. It is with this in mind that we proposed to explore other therapeutic avenues to try to provide other solutions through traditional African medicine. The Aim of the Study: To evaluate the haemostatic and healing activity of a Vernonia conferta-based gel on postextractional wounds induced in Wistar rats. Methodology: An experimental study was conducted over a period of eight months during 2021 at the multidisciplinary laboratory of Galenic Pharmacy and Pharmaceutical Legislation of the Faculty of Medicine and Biomedical Sciences of the University of Yaoundé 1. A gel formulation test carried out in standard proportions for a 2% concentration such as the Elugel reference gel used as a positive control. To evaluate the haemostatic and healing activity, we made up three batches of five female wistar rats with a body mass of between 100 and 130 grams, acclimatised for three days before the experiment. This test consisted of performing an avulsion of the central incisor in the rat and then applying our active principle for 5 successive days on one of the batches and our reference gel on the other batch, while the last batch will be under observation receiving no treatment and considered as a negative control. The observation was carried out during 14 days while respecting the feeding conditions of the animals. The haemostatic activity was evaluated using a stopwatch or we took the bleeding time after tooth extraction in the constituted batches. The healing activity was evaluated by taking a tissue sample from the extraction site on days 3, 7 and 14 for histological analysis. Results: The aqueous extract-based gel, made in standard proportions at a 2% concentration like the reference gel used as a positive control, stopped bleeding almost instantly, and clot formation occurred within 1-3 minutes after application. As for the healing activity, the batch of rats that received our test gel showed a rapid re-epithelialization observed on day 14ème, testifying to the restitution of the tissue injured during avulsion. Conclusion: The adhesion of Vernonia conferta aqueous leaf gel to the post-extraction alveolar mucosa in vivo in wistar rats has a favourable profile, as it allows faster haemostasis and accelerated healing.

Protective Effect of Casticin on Experimental Skin Wound Healing of Rats.

The wound is known as damage to the skin structure by external stimuli, such as cut, bruises, and burn, which typically leaves the internal tissue exposed. The wound repair process when hampered leads to an excessive burden on the healthcare setting. Therefore, there is an urgent need to develop effective agents that can promote the wound healing process. In the present study, we intended to investigate the pharmacological benefit of Casticin (CST), a polymethylflavone against wound. The wound in Wistar rats was induced by a surgical procedure. After surgery, the wound was examined for wound size over a period and for the expression of cyclooxygenase-2 and induced collagen III expressions. The effect of CST was examined on tumor necrosis factor α, interferon gamma, interleukin (IL)-10, transforming growth factor beta, and IL-7 by real-time polymerase chain reaction. The expression of matrix metalloproteinases (MMP)-2 and MMP-9 and its natural inhibitor (tissue inhibitors of metalloproteinases, TIMP1), level of macrophages, and lymphocytes were also quantified. The effect of CST was determined also on apoptosis of rats' splenocytes. CST significantly enhances wound healing of rat postsurgery, with maximum activity achieved in the case of a 60μM treated group. The expression of cyclooxygenase-2 was found reduced together with an increase in collagen III, tumor necrosis factor α, interferon gamma, IL-10, transforming growth factor beta, and IL-7 in the CST group. The levels of MMP-2 and MMP-9 were also found reduced together with an increase in TIMP1 level in CST-treated group. The levels of CD4+, CD8+, and CD11b+ cells at the wound site 24 and 120h postsurgery was also found reduced in CST-treated group. However, it showed no apoptosis in murine splenocytes. Collectively, CST can promote the wound healing process by modulation of inflammation and immune response, which induces tissue remodeling.

Novel fabrication of bi-metal oxide hybrid nanocomposites for synergetic enhancement of in vivo healing and wound care after caesarean section surgery.

In the current study, bi-metal oxide hybrid nanocomposites prepared by cerium oxide (CeO2 ) nanoparticles are included into chitosan-ZnO composites for developing the potential materials of dressing the wound. The wound healing effect of prepared hybrid nanocomposites was evaluated regarding the surface morphology, functional groups, thermal degradation and composite size. The antimicrobial activity of chitosan-ZnO/CeO2 hybrid nano composites was tested against the pathogens of Staphylococcus aureus and Escherichia coli. The hybrid nanocomposites containing CeO2 -based chitosan and ZnO nanoparticles were taken for optimum dressing included in the vivo studies on the excisional wounds in wistar rats. After 2 weeks, it is seen that the wound treated with CS-ZnO/CeO2 hybrid nano composites consists of the significant dressing of nearly 100% compared with control which showed nearly 65% of wound closure. Finally, our reported results gave the proof in supporting the availability of CS-ZnO/CeO2 hybrid nanocomposites contains the dressing of the wounds for the treatment.

Aloe Vera Gel Accelerates Wound Healing in Oral Mucosa by Promoting Epithelialization and Cell Proliferation (Fibroblasts)

Aim: To evaluate the accelerating effects of Aloe vera gel on the healing process of tongue wounds. Study design: Experimental animal study. Place and duration of study: Department of Anatomy, Postgraduate Medical Institute (PGMI), Lahore Pakistan from 29th April 2021 to 29th October 2021. Methodology: Forty two albino rats were divided into one control (group A) and two experimental groups (groups B &amp; C) with 14 rats in each group. Each group was divided into two sub groups with 7 rats each. Surgical excisional wounds were inflicted on anterior 2/3rd of dorsal surface of the tongue of each rat on day 1 of the study. The control group animals were given distilled water while animals in the experimental groups were given two different doses of Aloe vera gel daily (300 mg/kg and 500 mg/kg). Results Histological evaluation of the wounds revealed that epithelialization and fibroblast cell proliferation were promoted in Aloe vera groups as compared with the control groups. Healing time was significantly decreased in group B and C animals as compared with group A. Aloe vera gel accelerated healing in experimental groups. Conclusion: Aloe vera gel accelerates healing in tongue mucosa wounds in Wistar rats and it plays its role by bringing enhanced epithelialzation and fibrosis. Keywords: Haematoxylin &amp; Eosin (H&amp;E), Immune Histochemical (IHC), Effect

Healing Effects of Iranian Dwarf Elder on Full-thickness Epidermal Thermal Injury in Wistar Rats

Background: Wound healing is a physiologic process that cells and tissues react when the skin is exposed to thermal injury. The inflammatory process has ‎essential effects on wound healing that includes coordination between immunological and biological responses. Chemical and herbal medicines are locally or systemically administrated to help wound healing. Herbal medicines have become more prevalent in recent years due to fewer side effects. Dwarf elder is used to treating inflammation and has been reported to possess antibacterial and antioxidant activities. This plant is also effective against burns, wounds, eczema, and infection. Objectives: In this study, we evaluated the topical application of dwarf elder on full-thickness epidermal thermal wounds in Wistar rats. Methods: Thirty adult male Wistar rats of about 300 g were used. The rats were randomly divided into three groups: Control group, vehicle group treated with an ointment base, and treatment group treated with elder ointment 10%. The animals were anesthetized and were created burn wounds with an area of 1 cm2 using the Meyer method. After 21 days, the wounds were photographed with a Canon camera. The wound area and healing degree were calculated. Slides were stained with H&amp;E and examined by light microscopy and GraphPad Prism software. Data were analyzed by SPSS software using ANOVA and Tukey’s test. The significance level was considered at P &lt; 0.05. Results: The wound area on the 21st day was significantly lower in the treatment group than in the control and vehicle groups. The epithelium thickness (μm) was significantly higher in the treatment group than in the control and vehicle groups. The blood vessels were significantly higher in the treatment group than in the other groups. Conclusions: The study showed that elder extract could be an effective remedy to repair and heal thermal injury and burn wounds.

Effectiveness of Ethanol Extract Mangosteen Peel (Garcinia Mangostana L.) in Accelerating Post-Tooth Extraction Healing in Wistar Rats

Tooth extraction will cause a wound in the form of alveolar bone that opens in the oral cavity. Mangosteen peel has various pharmacological effects such as anti-inflammatory, antioxidant, antidiabetic, and antibacterial. The goal of this study was to find out the effectiveness of Mangosteen Peel Ethanol Extract (Garcinia mangostana L.) 45% with 90% in speeding up the healing time of post-tooth extraction wounds in Wistar rats. This type of research is an experimental laboratory with a post-test pattern only control group design. Conducted at the Laboratory of Pharmacology &amp; Laboratory of Traditional Medicine Faculty of Pharmacy, University of North Sumatra January-March 2021. The animal is a male Wistar rat of 32 who is physically healthy. The size of the sample is determined by Federer's formula, the minimum sample size for each treatment is 16 rats. The results of the study that there is a significant relationship between the amount of fibroblast tissue per field of view in Wistar Rats after tooth extraction with the administration of mangosteen peel (Garcinia mangostana L.) with a concentration of 45% and mangosteen peel (Garcinia mangostana L.) concentration 90%, p = 0.036 (p&lt;0.05). Mangosteen skin extract (Garcinia mangostana L.) is 45% and 90% effective in speeding up wound healing time after extraction of rat teeth Wistar. Mangosteen skin extract (Garcinia mangostana L.) is 90% more effective than turmeric extract (Curcuma longa) 45% in speeding up wound healing time post-extraction of rat teeth Wistar due to the flavonoid content in mangosteen skin extract (Garcinia mangostana L.) 90% which helps accelerate wound healing higher than skin extract Mangosteen (Garcinia mangostana L.) 45%.