Synergic effect of bone marrow derived mesenchymal stem cells and differentiated keratinocytes-like cells with a novel cellulose and collagen nanoscaffold on wound healing in rats

Abstract

Profound wounds could lead to massive infection or organ malfunction. The natural physiological process might fail to regenerate the skin flawlessly. On the contrary, conventional therapies have numerous drawbacks. Tissue engineering could offer a substitute therapy for wound regeneration. During this study, a pre-synthesized novel nano scaffold made of nano cellulose, collagen type I, and Carboxymethyl diethyl amino ethyl cellulose was used in combination with cell therapy to observe their synergic effect on wound healing in rats. Bone marrow-derived mesenchymal stem cells were differentiated into keratinocytes-like cells using EGF, BMP4, and FGF7. Differentiation was confirmed with real-time PCR and Immunocytochemistry. The scaffold alone and in combination with mesenchymal stem cells and differentiated cells were used to dress induced wounds in Wistar rats. The results showed the scaffold alone and in combination with cell therapy could assist in wound healing. The most effective treatment was the combination of the scaffold and differentiated cells.