Wound Dressings Research Articles

Synergetic effects of tetracycline hydrochloride incorporated regenerated cellulose acetate - Bacterial cellulose hybrid nanocomposite: Potential in biomedical application

Litter from cigarette waste is a significant threat to organisms and ecosystems. However, this waste contains cellulose acetate (CA) that can be recycled into raw materials. In this study, recycled CA from cigarettes (CFCA) electrospun through electro-spinning technique and developed hybrid nanocomposite by incorporating CFCA in the fermentation media, followed by self-assembly of bacterial cellulose (BC). CFCA exhibit excessive hydrophobicity due to their high crystallinity and reorientation of hydrophobic groups. We aimed to improve the hydrophilic, thermal and mechanical properties of CFCA. We examined fiber morphology using a scanning electron microscope (SEM), Fourier Transform Infrared Spectroscopy (FTIR), X-ray Diffraction Analysis (XRD), thermogravimetric analysis (TGA), swelling capacity and mechanical properties. BC/CFCA showed higher swelling capacity, improved thermal properties, and good tensile strength compared to CFCA. Additionally, tetracycline hydrochloride (TC) was loaded into developed BC/CFCA matrix and evaluated in-vitro drug release, antibacterial activity and cytotoxicity. In-vitro drug release results showed that developed BC/CFCA can able to control TC release. In addition, prepared BC/CFCA-TC composites demonstrated excellent antibacterial activity against gram-positive and gram-negative bacteria. More importantly, BC/CFCA-TC composites exhibit good cytotoxicity on mouse fibroblast cells (L929). These characteristics of BC/CFCA-TC membranes indicate they may successfully serve as wound dressings and other medical biomaterials.

Preparation of biocompatible Zein/Gelatin/Chitosan/PVA based nanofibers loaded with vitamin E-TPGS via dual-opposite electrospinning method

Wound management is a critical aspect of healthcare, necessitating effective and innovative wound dressing materials. Many existing wound dressings lack effectiveness and exhibit limitations, including poor antimicrobial activity, toxicity, inadequate moisture regulation, and weak mechanical performance. The aim of this study is to develop a natural-based nanofibrous structure that possesses desirable characteristics for use as a wound dressing. The chemical analysis confirmed the successful creation of Zein (Ze) (25% w/v) /gelatin (Gel) (10% w/v) /chitosan (CS) (2% w/v) /Polyvinyl alcohol (PVA) (10% w/v) nanofibrous scaffolds loaded with vitamin E tocopheryl polyethylene glycol succinate (Vit E). The swelling percentages of nanofiber (NF), NF + Vit E, cross-linked nanofiber (CNF), and CNF + Vit E were 49%, 110%, 410%, and 676%, respectively; and the degradation rates of NF, NF + Vit E, CNF, and CNF + Vit E were 29.57 ± 5.06%, 33.78 ± 7.8%, 14.03 ± 7.52%, 43 ± 6.27%, respectively. The antibacterial properties demonstrated that CNF impregnated with antibiotics reduced Escherichia coli (E. coli) counts by approximately 27–28% and Staphylococcus aureus (S. aureus) counts by about 34–35% compared to negative control. In conclusion, cross-linked Ze/Gel/CS/PVA nanofibrous scaffolds loaded with Vit E have potential as suitable wound dressing materials because environmentally friendly materials contribute to sustainable wound care and controlled degradation ensures wound dressings breakdown harmlessly.

Development of low-molecular-weight polysaccharide-based wound dressings for full-thickness cutaneous wound healing via coacervate formation

The development of polysaccharide-based wound dressings that are easy to prepare, adhere to tissue, adapt to diverse shapes and exhibit tunable mechanical properties holds significant clinical interest. This study introduced a simple spontaneous liquid-liquid phase separation technique employing low-molecular-weight and high polyion concentration of chitosan (CS) and hyaluronic acid (HA) to fabricate CS/HA coacervates. Upon increasing the molecular weight of chitosan from 7 kDa to 250 kDa, a transition in the CS/HA coacervates from liquid-like state to an elastic liquid and eventually to a solid-like state was observed. The resulting CS/HA coacervates demonstrated robust water resistance and adhesion to skin tissue. Notably, the molecular weight of chitosan significantly influenced the mechanical properties and hydration levels of the CS/HA coacervates. Moreover, in vivo studies using a full-thickness cutaneous defect model revealed that the CS/HA coacervates, prepared using 100 kDa chitosan, markedly accelerate wound healing. The coacervates' ease of preparation, wet adhesion, heterogeneous structure, suitability for irregularly shaped wounds, and exceptional wound healing promotion of the coacervates qualify them as an optimal wound dressing.

Fabrication and Properties of Hydrogel Dressings Based on Genipin Crosslinked Chondroitin Sulfate and Chitosan.

Multifunctional hydrogel dressings remain highly sought after for the promotion of skin wound regeneration. In the present study, multifunctional CHS-DA/HACC (CH) hydrogels with an interpenetrated network were constructed using hydroxypropyl trimethyl ammonium chloride modified chitosan (HACC) and dopamine-modified chondroitin sulfate (CHS-DA), using genipin as crosslinker. The synthesis of HACC and CHS-DA was effectively confirmed using Fourier transform infrared (FT-IR) analysis and 1H nuclear magnetic resonance (1H NMR) spectroscopy. The prepared CH hydrogels exhibited a network of interconnected pores within the microstructure. Furthermore, rheological testing demonstrated that CH hydrogels exhibited strong mechanical properties, stability, and injectability. Further characterization investigations showed that the CH hydrogels showed favorable self-healing and self-adhesion properties. It was also shown that increasing HACC concentration ratio was positively correlated with the antibacterial activity of CH hydrogels, as evidenced by their resistance to Escherichia coli and Staphylococcus aureus. Additionally, Cell Counting Kit-8 (CCK-8) tests, fluorescent images, and a cell scratch assay demonstrated that CH hydrogels had good biocompatibility and cell migration ability. The multifunctional interpenetrated network hydrogels were shown to have good antibacterial properties, antioxidant properties, stable storage modulus and loss modulus, injectable properties, self-healing properties, and biocompatibility, highlighting their potential as wound dressings in wound healing applications.

Capparis sepiaria-Loaded Sodium Alginate Single- and Double-Layer Membrane Composites for Wound Healing.

Background: Effective wound dressing is the key solution to combating the increased death rate and prolonged hospital stay common to patients with wounds. Methods: Sodium alginate-based single- and double-layer membranes incorporated with Capparis sepiaria root extract were designed using the solvent-casting method from a combination of polyvinyl alcohol (PVA), Pluronic F127 (PF127), and gum acacia. Results: The successful preparation of the membranes and loading of the extract were confirmed using Fourier transform infrared spectroscopy (FTIR) and X-ray diffraction (XRD). The prepared membranes were biodegradable and non-toxic to human skin cells (HaCaT), with high biocompatibility of 92 to 112% cell viability and good hemocompatibility with absorbance ranging from 0.17 to 0.30. The membrane's highest water vapor transmission rate was 1654.7333 ± 0.736 g/m2/day and the highest % porosity was 76%. The membranes supported cellular adhesion and migration, with the highest closure being 68% after 4 days compared with the commercial wound dressings. This membrane exhibited enhanced antimicrobial activity against the pathogens responsible for wound infections. Conclusions: The distinct features of the membranes make them promising wound dressings for treating infected wounds.

Semi-Interpenetrating Hydrogel with Long-Term Intrinsic Antibacterial Properties Promotes Healing of Infected Wounds In Vivo.

Bacterial infections significantly deteriorate the process of wound healing. The wound dressings loaded with antimicrobials are widely used to reduce bacterial infections. However, release-based sterilization may increase the risk of drug resistance of bacteria and complicate translation. Thus, the development of long-term intrinsic antibacterial wound dressings is highly desirable. In this study, an intrinsic antibacterial hydrogel (PVA/PPG-HBPL) consisting of poly(vinyl alcohol) (PVA), poly(polyethylene glycol methyl ether methacrylate-co-glycidyl methacrylate) (PPG), and hyperbranched poly-l-lysine (HBPL) was designed and fabricated. The mechanical properties of the PVA/PPG-HBPL hydrogel were enhanced by hydrogen bonding and semi-interpenetrating networks. It also possessed a favorable ability to absorb the wound exudates. The release of antibacterial HBPL was significantly decreased by the methods of cyclic freeze-thawing and covalent cross-linking during hydrogel fabrication, enabling the PVA/PPG-HBPL hydrogel with intrinsic and long-term antibacterial performance. The PVA/PPG-HBPL hydrogel dressing killed 99.9% of methicillin-resistant Staphylococcus aureus (MRSA) cultured on its surface without observable cytotoxicity in vitro. It observably shortened the healing process by 2 orders of magnitude of MRSA colonies compared with the control in the MRSA-infected full-thickness skin wound of rats in vivo even after being soaked in phosphate-buffered saline (PBS) for 21 days (PBS was changed every 3 days). The antibacterial hydrogels could kill wound bacteria in a timely manner, significantly reduce inflammatory cell infiltration, and promote neovascularization and collagen deposition.

Influence of polylactide wound dressings with tannic acid on serum concentration of markers of angiogenesis, endothelial cell alteration and activation in white rats during healing of experimental full-thickness skin defect

Influence of polylactide wound dressings with tannic acid on serum concentration of markers of angiogenesis, endothelial cell alteration and activation in white rats during healing of experimental full-thickness skin defect

Double Enzyme Active Hydrogel Program Regulates the Microenvironment of Staphylococcus aureus-Infected Pressure Ulcers.

The treatment of infected pressure ulcers (IUPs) requires addressing diverse microenvironments. A pressing challenge is to effectively enhance the regenerative microenvironment at different stages of the healing process, tailoring interventions as needed. Here, a dual enzyme mimetic and bacterial responsive self-activating antimicrobial hydrogel designed to enhance IPUs healing is introduced. This hydrogel incorporates pH-responsive dual enzyme-active nanoplatforms (HNTs-Fe-Ag) encapsulated within a methacrylate-modified silk fibroin (SFMA) and dopamine methacrylamide (DMA) matrix. This composite hydrogel exhibits adaptive microenvironment regulation capabilities. Under the low pH microenvironment of bacterial infection, it has excellent antimicrobial activity by self-activating the •OH generation in conjunction with photothermal effects. Under the neutral and alkaline microenvironment of chronic inflammation, it catalyzes the decomposition of hydrogen peroxide (H2O2) to produce oxygen (O2), thereby alleviating hypoxia and scavenging reactive oxygen species (ROS), which in turn remodulates the phenotype of macrophages. The composite hydrogel demonstrates on-demand therapeutic effects in the microenvironment of infected wounds, significantly enhancing the regenerative microenvironment of IUPs by promoting wound closure, inflammation regulation, and collagen deposition through self-activated antimicrobial action during infection and adaptive hypoxia relief during recovery. This approach offers a novel strategy for developing smart wound dressings.

Soft hydrogels obtained by photothermal curing of poly(vinylpyrrolidone)/gold nanoparticles dispersions showing anisotropic swelling and photo-activated antimicrobial properties

Poly(vinylpyrrolidone) (PVP) hydrogels were obtained by green light irradiation of aqueous PVP/ammonium persulfate solutions modified with gold nanoparticles (AuNPs). The increase in temperature produced through the activation of the photothermal effect of AuNPs triggers the decomposition of ammonium persulfate producing radicals that, by attacking the polymer main chain and generating macro-radicals, induce the crosslinking of the polymer via recombination paths. Irradiation from the top of the solutions in an open configuration produced water evaporation and a concomitant increase in temperature during curing, giving rise to dry materials with high aspect ratio and moderate swelling anisotropy. Pads that reversibly adhere to different substrates, including human skin, were produced by simple contact of the final materials with water. Impregnation of gauze wound dressings with PVP-Au-ammonium persulfate dispersions, followed by direct “in situ” photothermal crosslinking, enabled the fabrication of supported functional pads with potential applications as reversible adhesive skin wound dressings. Infusion of the hydrogels with Rose Bengal (RB) provides the materials with photosensitizing properties useful for the photodynamic inactivation of Staphylococcus aureus. A decrease in cell viability of 99.9% was attained after 30 min of irradiation with a low-power green LED source, which demonstrates the efficient singlet oxygen production by RB in the hydrogels and paves the way towards the design of new stimulus-activated bactericidal supplies for biomedical uses.

3D-Printed Methacrylated Gelatin–Lignin Carbon Dot Hydrogel Combined with PLA Nanofibers for Wound Dressings

3D-Printed Methacrylated Gelatin–Lignin Carbon Dot Hydrogel Combined with PLA Nanofibers for Wound Dressings

Immunomodulatory Hydrogel for Electrostatically Capturing Pro-inflammatory Factors and Chemically Scavenging Reactive Oxygen Species in Chronic Diabetic Wound Remodeling.

Diabetic wound exhibits the complex characteristics involving continuous oxidative stress and excessive expression of pro-inflammatory cytokines to cause a long-term inflammatory microenvironment. The repair healing of chronic diabetic wounding is tremendously hindered due to persistent inflammatory reaction. To address the aforementioned issues, here, a dual-functional hydrogel is designed, consisting of N1-(4-boronobenzyl)-N3-(4-boronophenyl)-N1, N1, N3, N3-tetramethylpropane-1, 3-diaminium (TSPBA) modified polyvinyl alcohol (PVA) and methacrylamide carboxymethyl chitosan (CMCSMA) can not only electrostatically adsorb proinflammatory cytokines of IL1-β and TNF-α, but can also chemically scavenge the excessive reactive oxygen species (ROS) in situ. Both in vitro and in vivo evaluations verify that the negatively charged and ROS-responsive hydrogel (NCRH) can effectively modulate the chronic inflammatory microenvironment of diabetic wounds and significantly enhance wound remodeling. More importantly, the well-designed NCRH shows a superior skin recovery in comparison with the commercial competitor product of wound dressing. Consequently, the current work highlights the need for new strategies to expedite the healing process of diabetic wounds and offers a wound dressingmaterial with immunomodulation.

An open label, single-centric, post market clinical study to evaluate the safety and efficacy of a new antimicrobial wound dressing (VELVERT) as an adjuvant therapy in the treatment of venous leg ulcer.

An open label, single-centric, post market clinical study was undertaken to evaluate the safety and efficacy of a new antimicrobial wound dressing (VELVERT) as an adjuvant therapy in the treatment of venous leg ulcer (VLU). Patients with VLU of grade C-5 according to CEAP classification and above were evaluated using doppler ultra sound. The efficacy of new antimicrobial wound dressing (VELVERT) was assessed in terms of wound area reduction within a time frame of 60 days and surgeon questioners. Patients were evaluated for VELVERT safety and pain level on a scale of 0-10 Numeric Pain Chart. Presence of micro-organism load was monitored at regular time interval. VELVERT treatment was effective as 71.43% reduction in the ulcer area was observed. After 60 days, a total of 9 (45%) patients had complete ulcer closure. A remarkable decrease in the severity of pain was observed with 11 (55%) patients expressing no pain at the EOT. Swab test showed negative result for micro-organism growth. No serious adverse events were observed during the trial. The data indicates that VELVERT is an effective treatment for VLUs and showed the potential in the wound care of VLUs.

Bacterial cellulose nanofiber reinforced self-healing hydrogel to construct a theranostic platform of antibacterial and enhanced wound healing

In order to promote wound healing, self-healing hydrogels with moisturizing property are employed as wound dressing. In this study, bacterial cellulose nanofibers (BCN) with high mechanical strength are used as reinforcement to improve the mechanical properties of self-healing hydrogels. A multifunctional self-healing hydrogel has been constructed by incorporating natural biomass, including Ag hybrid bacterial cellulose nanofiber (Ag-BCN), resveratrol (Res), and carbon nanodots (CNDs). The results of in vitro experiments demonstrate that the mechanical strength of the hybrid hydrogel was increased by 6 times with the addition of Ag-BCN, which also offers excellent antibacterial efficiency (S. aureus 99.99 % and E. coli 99.68 %). The hydrogel with CNDs can observe the healing process of the crack in real time and realize the controlled release of Res through photothermal effect. Moreover, the results of animal model experiments indicate that the prepared hydrogel could reduce the infection of the wound, effectively shorten the progress of wound healing (from 21d to 14 d). All the results imply that the prepared hydrogel has great promise in the application of skin wound healing.

REAÇÕES CUTÂNEAS GRAVES: UMA VISÃO ABRANGENTE SOBRE A SÍNDROME DE STEVENS-JOHNSON, SUA ETIOLOGIA, FISIOPATOLOGIA E ABORDAGENS TERAPÊUTICAS

Stevens-Johnson Syndrome (SJS) is a rare and severe condition characterized by epidermal detachment and mucosal lesions, primarily affecting the mouth, eyes, and genitals. Often triggered by adverse drug reactions, such as antibiotics and anticonvulsants, SJS can also be caused by viral infections and, rarely, neoplasms. SJS forms a spectrum with Toxic Epidermal Necrolysis (TEN), differing in the extent of skin involvement: in SJS, less than 10% of the body surface is affected, while in TEN, the involvement exceeds 30%. Although rare, with an incidence of 1.2 to 6 cases per million people, SJS carries high morbidity and mortality, leading to complications such as severe infections and permanent damage to the skin and mucous membranes. Early diagnosis is crucial, based on characteristic lesions and a recent history of medication use. In some cases, a biopsy is necessary to confirm the diagnosis. Treatment involves a multidisciplinary approach, including intensive care, immediate discontinuation of the causative drug, and immune modulation with corticosteroids or intravenous immunoglobulin. While new therapies, such as cyclosporine and special wound dressings, are being explored, there is still a need for consensus on the ideal treatment protocol, as well as advances in personalized diagnosis and treatment.

Polyelectrolytes Complex-Based Hydrogels Derived from Natural Polymers and Cannabinoids for Applications as Wound Dressing

Polyelectrolytes Complex-Based Hydrogels Derived from Natural Polymers and Cannabinoids for Applications as Wound Dressing

Optimization through response surface modelling, experimental validation on development of nanocellulose for pharmaceutical applications

Nanocellulose, a promising polymer derived from lignocellulosic sources, is utilized in various applications such as paper production, water purification, wound dressing, scaffolds, biosensors, super-disintegrants, cosmetics, and drug delivery systems. The study investigates the production of optimized nanocellulose size using response surface methodology, examining the impact of factors like sulphuric acid concentration and temperature on the acid hydrolysis process. The central composite design was used to screen and adjust the design matrix with two-factor levels. The optimized size of nanocellulose was found to be 364.1 nm, with a zeta potential of −40.6, which shows long-term stability. Hence, process variables like sulphuric acid of 48.29% v/v and temperature of 39.7 °C were optimized to get the desired particle size from commercial cellulose. An Analysis of Variance (ANOVA) was applied to determine the primary parameters that have a significant impact on the particle size of nanocellulose. Thus, the obtained nanocellulose was characterized using FTIR, XRD, DLS and TEM analysis. FTIR confirms that the functional groups of cellulose are similar in nanocellulose. As the XRD illustrates, 67% of the crystallinity index in the developed nanocellulose is semicrystalline. The particle size was found within the nm size by employing the DLS method. Nanocellulose was characterized using TEM for surface morphology. Thus, obtained nanocellulose is widely used in various pharmaceutical applications like tissue engineering, cosmeceuticals, wound healing, scaffolds, aerogels, hydrogels, and controlled release of drugs.

Antibacterial gelatin/tragacanth gum films containing galbanum essential oil for in vitro scratch-healing

Natural substances and bioactive agents possess great potential in wound care based on their ability to promote healing and prevent infection. This study focused on the fabrication of antibacterial wound dressings by combining gelatin (G), tragacanth gum (TG), and galbanum essential oil (GEO) as a loaded drug. TG addition resulted in more elastic and flexible films besides enabling encapsulation of the hydrophobic GEO into the biopolymeric matrix. GEO was utilized as an antibacterial and a wound-healing enhancer for open wounds such as incisions. Field emission scanning electron microscopy (FE-SEM) analysis revealed a porous film structure after GEO incorporation. Higher GEO concentration caused reduced swelling and slower degradation. Water vapor transfer rate varied from 596 to 894 g/m2.day, making the films suitable for wound dressings. GEO release exhibited a two-phase profile with prolonged diffusion-controlled release for a higher content of GEO. The films demonstrated dose-dependent antimicrobial activity against S. aureus and E. coli strains. Effectiveness and noteworthy application of this research were approved by scratch assay on human dermal fibroblast cells, and films with 3 % GEO showed 79.42 % wound closure, which is significantly higher than the control sample (55.15 %), indicating promoted cell migration and promising wound healing properties.

Injectable Hydrogel With Glycyrrhizic Acid and Asiaticoside-Loaded Liposomes for Wound Healing.

Open skin wounds increase the risk of infections and can compromise health. Therefore, applying medications to promote healing at the injury site is crucial. In practice, direct drug delivery is often difficult to maintain for a long time due to rapid absorption or wiping off, which reduces the efficiency of wound healing. Consequently, the development of bioactive materials with both antibacterial and wound-healing properties is highly desirable. This study synthesized liposomes loaded with glycyrrhizic acid (GA) and asiaticoside (AS) by film dispersion-ultrasonication method, which were then incorporated into a GelMA solution and cross-linked by ultraviolet light to form a bioactive composite hydrogel for wound dressings. This hydrogel is conducive to the transport of nutrients and gas exchange. Compared with GelMA hydrogel (swelling rate 69.8% ± 5.7%), the swelling rate of GelMA/Lip@GA@AS is lower, at 52.1% ± 1.0%. GelMA/Lip@GA@AS also has better compression and rheological properties, and the invitro biodegradability is not significantly different from that of the collagenase-treated group. In addition, the hydrogel polymer has a stable drug release rate, good biocompatibility, and an angiogenic promoting effect. Invitro experiments prove that, at concentrations of 0.5, 1, 2, and 3 mg/mL, GelMA/Lip@GA@AS can inhibit the growth of Staphylococcus aureus. We synthesized GelMA/Lip@GA@AS hydrogel and found it possesses advantageous mechanical properties, rheology, and biodegradability. Experimental results invitro showed that the bioactive hydrogel could efficiently release drugs, exhibit biocompatibility, and enhance angiogenesis and antimicrobial effects. These results suggest the promising application of GelMA/Lip@GA@AS hydrogel in wound-dressing materials.

Structure regulation of oxidized soybean cellulose nanocrystals/poly-acrylamide hydrogel and its application potential in wound dressing

The development of functional dressings based on natural polysaccharide-based hydrogels remains a great challenge, and the specific roles of gel composition and drug-controlled release mechanisms were unclear. In this study, oxidized soybean cellulose nanocrystals (CNCs)/poly-acrylamide (PAM) hydrogel was prepared. The proportion of CNCs, crosslinkers, and water in the system was regulated to fine-tune the rheological performances, texture properties, transparency, and micro-network structures of CNCs/PAM hydrogel, and further explored its application potential in the field of wound dressings. It was found that CNCs improved the rigidity and adhesion of hydrogels, crosslinkers improved the network density, and water promoted the softening and fluidity of hydrogels. The effective filling of CNCs in the composite hydrogel was verified by FTIR, XRD, and NMR. Furthermore, the pH responsiveness and drug-loading potential of the smart hydrogel were tested by swelling and drug-controlled release experiments, elucidating drug-release dynamic mechanisms during the wound healing process. The inhibition zones (>7 mm) of gram-positive/negative bacteria and cell viability (>100 %) assay showed satisfactory biocompatibility, as the hydrogel effectively accelerated wound healing in a wound model. These results elucidated the regulation mechanism of the structures of CNCs/PAM hydrogel and revealed the application potential of CNCs/PAM hydrogel in wound dressings.

Multifunctional poloxamer-based thermo-responsive hydrogel loaded with human lactoferricin niosomes: In vitro study on anti-bacterial activity, accelerate wound healing, and anti-inflammation

Chronic wound infections are attributed to delayed tissue repair, which remains a major clinical challenge in long-term health care. Particularly, infections with antibiotic resistance have more serious effects on health, often resulting in unsuccessful treatments. Thus, antimicrobial peptide (AMP)-based therapy holds promise as a potential therapeutic approach to overcoming drug resistance. Conventional wound dressing is a passive strategy for wound care that is not capable of eradicating pathogens and promoting tissue repair. In this study, we aim to construct an advanced wound dressing; a thermo-responsive hydrogel incorporated with lactoferricin (Lfcin) niosome (Lfcin-Nio/hydrogel) for bacterial pathogen treatment. The Lfcin-loaded niosome (Lfcin-Nio) has a particle size of 396.91 ± 20.96 nm, 0.38 ± 0.01 of PdI, −10.5 ± 0.3 mV of ζ potential, and 72.30 ± 7.05 % Lfcin entrapment efficiency. Lfcin-Nio exhibited broad antibacterial activity on both drug-susceptible and drug-resistant strains, and also on bacteria residing in the biofilm matrix. The Lfcin-Nio/hydrogel was fabricated from 0.5 % w/v poloxamer 188–20 % w/v poloxamer 407, and supplemented with Lfcin-Nio and epidermal growth factor (EGF). The physical properties of Lfcin-Nio/hydrogels showed elasticity, swelling ability, and strong injectability with responsiveness to 33–37 °C temperatures. The biological properties of Lfcin-Nio/hydrogels exhibited a bactericidal effect against drug-resistant strains of S. aureus and P. aeruginosa, and showed less toxicity to the human skin fibroblast. It also promoted the healing of scratches by 55 % within 6 h, compared to the wound closure rate of 20 % in the cell control. The inflammatory response of the Lfcin-Nio/hydrogel-treated cells was reduced via suppression of IL-1β and COX-2 mRNA expressions. From this study, Lfcin-Nio/hydrogels can be suggested as a modern wound dressing that possesses multifunctional and beneficial properties for the management of chronic wound infections.