Wormwood Extracts Research Articles

Treatment of Burn Wounds with a Chitosan-Based Hydrogel Dressing Containing Artemisia absinthium L.: A Comprehensive In Vivo Study

Objective: Wormwood (Artemisia absinthium L.) is a valuable medicinal plant that has been used for the wound healing since ancient times. Hydrogel dressings are often preferred for wound care in treatment. In this study, the effects of chitosan-based hydrogel dressings containing wormwood were investigated in rats with burn wound model for the first time. Material-Method: Certain phenolic compounds in wormwood extracts were detected by LC-MS/MS and antioxidant activities were calculated using the DPPH. The antimicrobial activity of the dressings was tested using the disk diffusion method. Tissues taken from 48 female Sprague-Dawley rats were histopathologically examined at day 3rd and 21st. Skin tissue cytokine levels were measured using ELISA. All data obtained from histopathological examination and cytokine levels were statistically evaluated. Results: Fifteen phenolic compounds were quantitatively determined in wormwood extracts. The antioxidant activities of high-, medium-, and low-dose wormwood extracts were as 91,1% ± 0,054, 89,6% ± 0,012 and 84,1% ± 0,02, respectively. The hydrogel dressings did not show any antimicrobial activity against S. aureus (ATCC 29213) or P. aeruginosa (ATCC 27853). Granulation tissue formation, collagen increase, and regular scar appearance were higher in the all three wormwood groups. Wound contraction was completed and remodeling phase started at day 21st, especially in the high-dose wormwood treatment group. Tissue cytokine levels were determined in pg/ml in all groups. Conclusion: It was determined that A. absinthium L. can promote wound healing through various mechanisms of action and shows immunomodulatory effects, and is appropriate for use as a wound dressing in the form of a chitosan-based hydrogel.

Wormwood-infused porous-CaCO3 for synthesizing antibacterial natural rubber latex

Wormwood leaf is a traditional Chinese herbal medicine with a high medicinal value and long application history and its essential oil is a high-purity plant oil extracted from Wormwood leaf. Pharmacological research reveals that Wormwood leaf and Wormwood essential oil are a broad-spectrum antibacterial and antiviral drug, which can inhibit and kill many bacteria and viruses. We loaded wormwood extract on porous calcium carbonate (Porous-CaCO3) and introduced it and Wormwood essential oil into Natural rubber latex (NRL), thus synthesizing NRL composites with excellent vitro and in vivo antibacterial effect, cell compatibility and mechanical properties. This NRL material can delay the light aging and thermal oxidation of some mechanical properties, which provides a broader avenue for its commercialization.

Recombinant Art v4.01 protein produces immunological tolerance by subcutaneous immunotherapy in a wormwood pollen-driven allergic asthma female mouse model.

Art v4.01 is a well-known profilin protein belonging to the pan-allergens group and is commonly involved in triggering allergic asthma, polyallergy, and cross-sensitization. It is also referred to as Wormwood due to its origin. Crude wormwood extracts are applied for allergen-specific immunotherapy (AIT). Whether the recombinant Art v4.01 (rArt v4.01) can produce in vivo immunological tolerance by subcutaneous immunotherapy (SCIT) remains elusive. In this study, to investigate the in vivo immunological response of rArt v4.01, Th2, Th1, Treg, Th17 type-related cytokines and phenotypes of immune cells were tested, facilitating the exploration of the underlying mechanisms. The expression and purification of Art v4.01 were carried out using recombinant techniques. Allergic asthma female BALB/c mice were induced by subcutaneous sensitization of wormwood pollen extract and intranasal challenges. SCIT without adjuvant was performed using the rArt v4.01 and wormwood pollen extract for 2 weeks. Following exposure to challenges, the levels of immunoglobulin E (IgE), cytokines, and inflammatory cells were assessed through enzyme-linked immunosorbent assay (ELISA) and histological examination of sera, bronchoalveolar lavage fluid (BALF), and lung tissue. These parameters were subsequently compared between treatment groups receiving rArt v4.01 and wormwood pollen extract. The rArt v4.01 protein was expressed, which had a high purity (>90%) and an allergenic potency. Compared with the pollen extract, rArt v4.01 was superior in terms of reducing the number of white blood cells (WBCs), total nucleated cells (TNCs), and monocytes (MNs) in BALF and the degree of lung inflammation (1.77±0.99 vs. 2.31±0.80, P > 0.05). Compared with the model group, only rArt v4.01 reduced serum IgE level (1.19±0.25 vs. 1.61±0.17 μg/ml, P = 0.062), as well as the levels of Th2 type-related cytokines (interleukin-4 (IL-4) (107.18±16.17 vs. 132.47±20.85 pg/ml, P < 0.05) and IL-2 (19.52±1.19 vs. 24.02±2.14 pg/ml, P < 0.05)). The study suggested that rArt v4.01 was superior to pollen extract in reducing the number of inflammatory cells in BALF, pneumonitis, levels of pro-inflammatory cytokines, and serum IgE level. These findings confirmed that Art v4.01 could be a potential candidate protein for allergen-specific immunotherapy.

Separation and Purification of Plant Extracts by Removal of Chlorophyll, Xanthophylls and Pigments using Mineral Adsorbents in Liquid Chromatography (CLS)

This study focused on the purification of plant extracts, with the help of adsorption chromatography (liquid chromatography with a solid stationary phase - CLS) so that the purified plant extracts lead to obtaining bioactive compounds in a pure state or with advanced purity. The studied extracts can be divide into categories as follows: a) The purification of extracts from perilla frutescens b) The purification of extracts from rosmarinus officinalis c) The purification of extracts from artemisia annua (peliniță) For the perilla frutescens extract, dried leaves, the purification aimed at obtaining pure crystals of ursolic acid and the physico-chemical analysis of the plant extracts before and after extraction, to determine the loss of the important compounds of the plant, due to their retention in the adsorbent layer. For the rosemary and wormwood (artemisia annua) extracts, dry leaves, the purification aimed at obtaining pure crystals of bioactive compounds (ursolic acid, artemisinin) and their gravimetric dosing to determine the bioactive compound content of the original plant. Mineral adsorbents and combinations of mineral adsorbents in the form of adsorbent mixtures have been used. Glass liquid columns of various sizes were used. For each experiment, the working times (saturation-activation time, elution and column washing time) were noted. All experiments were performed with the adsorption chromatography procedure, with gravity flow column (in adsorption chromatography with gravity flow column), without using Sephadex LH-20 macroporous resins as a stationary phase.

Study of antioxidants from herbal plant extracts in vitro

Abstract&#x0D; &#x0D; Background : Antioxidants are substances that can prevent the formation of free radical reactions, people in Indonesia use a lot of natural ingredients as traditional medicine, one of which as antioxidants for free radical handling . Free radicals are compounds or molecules that contain one or more unpaired electrons in its outer orbitals. The presence of unpaired electrons causes these compounds to be very reactive looking for partners, by attacking and binding electrons around them so that they can trigger disease. &#x0D; Destination : The purpose of this study was to discuss the antioxidant activity of wormwood, neem, cinnamon, brotowali plants in vitro. &#x0D; Method : The method that can be used in antioxidant test research was the DPPH method in vitro through effective concentration (EC50), identification of phytochemical screening of each extract, and total flavonoid test. &#x0D; Results : The results of the study obtained phytochemical screening of wormwood, neem, cinnamon, brotowali extracts contained secondary metabolites in the form of flavonoids, tannins, saponins, steroids and triterpenoids which have a role as antioxidanttest. Total flavonoid test of wormwood extract had the largest total flavonoid content (110.353 ± 0.802). The antioxidant test results of each cinnamon extract was 75 ppm, neem extract was 81 ppm, wormwood extract was 479 ppm, brotowali extract was 304 ppm. These results indicated that the effective antioxidant extract was cinnamon extract.

Перспективы использования водного и растительного видов сырья Волго-Каспийского региона в технологии приготовления рыбоовощных супов для геродиетического питания

The possibility of using pike-perch of the ordinary Volga-Caspian basin and vegetable raw materials (Jerusalem artichoke and wormwood herb) in the technology of fish and vegetable soups for gerodietic nutrition has been found. It was determined that in the mass composition of pike-perch caught by the fishing artel “Chelyuskinets” and harvested by the fish processing enterprise VES, LLC the muscle tissue (meat) during its butchering made 44.5–45.1 and 40.4%, respectively. Pike perch meat belongs to the protein (18.0–19.0%) low-fat (1.1%) type of raw material. It was found that in the tubers of Jerusalem artichoke Skorospelka growing in the Astrakhan region, the water content was 77.0%, protein 4.0%, carbohydrates 18.0%, while the energy value of the raw material was 88 kcal/100 g. It has been stated that 0.01% aqueous extract of the medicinal herb wormwood has good organoleptic properties: it is colorless, odorless, with a slightly astringent taste of wormwood. This variant of the extract was chosen for adding into the fish broth. There have been developed the formulations and prototypes of ready-to-eat fish and vegetable products: fish soup with minced pikeperch meatballs with wormwood extract, soup with minced pikeperch meatballs with potatoes in vegetable broth, soup with minced pikeperch meatballs with Jerusalem artichoke in fish and vegetable broth. Their organoleptic parameters of the product quality were studied, a significantly increased content of protein substances (7.7%) for the soup was found with a low fat content (1.1%) and low energy value (51.1 kcal/100 g), which allows classifying soup samples as functional gerodietic foodstuffs.

Enhancing Antibacterial Performance of Viscose Spunlaced Nonwovens by Wormwood Extract Microcapsule Finishing

Enhancing Antibacterial Performance of Viscose Spunlaced Nonwovens by Wormwood Extract Microcapsule Finishing

Antibacterial and Phytochemical Screening of Artemisia Species

Taking into account the increasing number of antibiotic-resistant bacteria, actual research focused on plant extracts is vital. The aim of our study was to investigate leaf and stem ethanolic extracts of Artemisia absinthium L. and Artemisia annua L. in order to explore their antioxidant and antibacterial activities. Total phenolic content (TPC) was evaluated spectrophotometrically. Antioxidant activity was evaluated by DPPH and ABTS. The antibacterial activity of wormwood extracts was assessed by minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) in Escherichia coli, Staphylococcus aureus, Listeria monocytogenes, and Salmonella enteritidis cultures, and by zone of inhibition in Klebsiella carbapenem-resistant enterobacteriaceae (CRE) and Escherichia coli extended-spectrum β-lactamases cultures (ESBL). The Artemisia annua L. leaf extract (AnL) exhibited the highest TPC (518.09 mg/mL) and the highest expression of sinapic acid (285.69 ± 0.002 µg/mL). Nevertheless, the highest antioxidant capacity (1360.51 ± 0.04 µM Trolox/g DW by ABTS and 735.77 ± 0.02 µM Trolox/g DW by DPPH) was found in Artemisia absinthium L. leaf from the second year of vegetation (AbL2). AnL extract exhibited the lowest MIC and MBC for all tested bacteria and the maximal zone of inhibition for Klebsiella CRE and Escherichia coli ESBL. Our study revealed that AbL2 exhibited the best antioxidant potential, while AnL extract had the strongest antibacterial effect.

Aqueous Extracts of Four Medicinal Plants and Their Allelopathic Effects on Germination and Seedlings: Their Morphometric Characteristics of Three Horticultural Plant Species

Allelopathy is a biological phenomenon of synthesizing and excreting compounds that can affect the growth of various organisms, i.e., plant species. The aim of this work was to investigate the possible allelopathic influence of aqueous extracts, obtained from plant species chia (Salvia hispanica L.), black cumin (Nigella sativa L.), wormwood (Artemisia absinthium L.), and nettle (Urtica dioica L.), on the seed germination growth and morphometric characteristics of pepper (Capsicum annuum L.), spinach (Spinacia oleracea L.) and lettuce (Lactuca sativa L.) seedlings in laboratory conditions. Pepper, spinach, and lettuce seedlings were treated with aqueous extracts of chia, nettle, wormwood, and black cumin of different concentrations (2.5, 5 and 10%, respectively). The aqueous extracts were prepared according to the method developed by Norsworthy and the qualitative and quantitative analysis of phenolic compounds in aqueous extracts were performed using ultra-performance liquid chromatography electrospray ionization tandem mass spectrometry (UPLC-ESI-MS/MS). Phytochemical composition of chia aqueous extracts showed the highest content (above the 1 mg L−1) of epicatechin, quinic acid, caffeic acid, esculetin and cinnamic acid in the comparison with others from the total of 19 detected chemical compounds. In aqueous extracts of black cumin, there were six compounds detected in content above 1 mg L−1, i.e., epicatechin, quinic acid, caffeic acid, esculetin, cinnamic acid, and kaempferol. The same compounds were the most abundant in wormwood extracts, along with gallic acid. Epicatechin and esculetin were only two compounds detected in aqueous extract of nettle in concentration higher than 1 mg L−1. According to the results of this study, only the treatment of spinach seeds with chia aqueous extract in concentration of 2.5% before germination stimulates the germination of spinach seeds, and wormwood herbs and chia extracts in concentrations of 2.5 and 5% stimulate the germination of lettuce seeds and the growth of hypocotyl and radicle length of developed seedlings. The treatment of pepper and lettuce seeds with aqueous extract of nettle in concentration of 10% completely inhibited seed germination.

Biologically active food components as an element in the prevention of coronavirus infection: A review

Immune cells play a vital role in protecting the human body from pathogens and viruses. The deficiency of a number of nutrients in the diet negatively affects the state of the immune system, which disrupts the chemical, structural, and regulatory processes in the body. The purpose of the article is to consider the role of various biologically active nutrients and bioactive substances in increasing innate and adaptive human immunity for the prevention of COVID-19 in the population. Using the research method of scientific electronic libraries, bibliographic bases of articles on medical sciences, it was found that such vitamins as C, D, A, and E, minerals (zinc, iron, selenium), resveratrol, turmeric flavonoids, garlic, green tea, elderberry extract, biologically active substances from an aqueous extract of wormwood leaves help prevent coronavirus infection or reduce the severity of the disease, having immunomodulatory, anti-inflammatory, antioxidant and antiviral properties. In the context of the ongoing pandemic of coronavirus infection, recommendations for including the biologically active substances described above in the diet can be extrapolated to the entire population to maintain the immune system and the body’s resistance to infection.

Antifungal Effect of Plant Extracts on the Growth of the Cereal Pathogen Fusarium spp.—An In Vitro Study

The aim of the study was a laboratory evaluation of the antifungal effect of leaf extracts from yarrow (Achillea millefolium L.), tansy (Tanacetum vulgare L.), sage (Salvia officinalis L.) and wormwood (Artemisia absinthium L.) on fungi of the genus Fusarium, major cereal pathogens. The study used 5%, 10%, and 20% concentrations of plant extracts, evaluating their effect on the linear growth of Fusarium avenaceum, F. culmorum, F. graminearum, F. sporotrichioides and the percentage of their growth inhibition compared to control. The study also included the assessment of the content of selected biologically active compounds in plant extracts and their impact on the development of the aforementioned pathogenic fungi. The total content of polyphenols and flavonoids in the extracts was assessed by spectrophotometry, and antioxidant activity was determined using the synthetic 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical. Plant extracts from sage were characterized by the highest polyphenol contents (81.95 mg/mL) and flavonoids (21.12 mg/mL) compared to other plant extracts, and also showed the highest antioxidant activity (102.44 mM Trolox). Wormwood extract contained the lowest amount of phenolic compounds (flavonoids—5.30 mg/mL, polyphenols—43.83 mg/mL). Plant extracts inhibited the mycelia growth of fungal pathogen depending upon the fungus species, type of extract and its concentration. The extracts of sage (S) and tansy (T) plants at a concentration of 20% demonstrated strong inhibitory effect against the tested fungi (the highest inhibition coefficient for S20: 83.53%; T20: 72.58%), while 10% and 5% extracts of these plants were less effective in inhibiting the growth of Fusarium (highest inhibition coefficient for S10: 71.33%; S5: 54.14%; T10: 56.67%; T5: 38.64%). Yarrow (Y) and wormwood (W) extracts showed low fungistatic effect. Their 20% concentration inhibited the development of mycelia growth of fungi at the level of 63.82% (W20) and 67.57% (Y20). The 5% and 10% concentrations of these plant extracts had the weakest effect on the tested fungi (Y5: 34.09; W5: 42.06%; Y10: 45.01%; W10: 57.44%), even stimulating the Fusarium growth compared to the control (Y5: −23.7%). Based on the study, it was found that each species of fungus reacted differently to the addition of the extract to the culture medium and its concentration, however F. avenaceum and F. culmorum were the most sensitive fungi, while the least sensitive was F. graminearum. The results of the research are the preliminary phase for further field tests to determine the fungistatic effect of plant extracts in field conditions, their phytotoxicity and biological stability, as well as the possibility of producing a biopreparation to protect plants against fusariosis.

Synthesis and Characterization of Bioactive Magnetic Nanoparticles from the Perspective of Hyperthermia Applications

Magnetic iron oxide nanoparticles were obtained for the first time via the green chemistry approach, starting from two aqueous extracts of wormwood (Artemisia absinthium L.), both leaf and stems. In order to obtain magnetic nanoparticles suitable for medical purposes, more precisely with hyperthermia inducing features, a synthesis reaction was conducted, both at room temperature (25 °C) and at 80 °C, and with two formulations of the precipitation agent. Both the quality and stability of the synthesized magnetic iron oxide nanoparticles were physiochemically characterized: phase composition (X-ray powder diffraction (XRD)), thermal behavior (thermogravimetry (TG) and differential scanning calorimetry (DSC)), electron microscopy (scanning (SEM) and transmission (TEM)), and magnetic properties (DC and HF-AC). The magnetic investigation of the as-obtained magnetic iron oxide nanoparticles revealed that the synthesis at 80 °C using a mixture of NaOH and NH3(aq) increases their diameter and implicitly enhances their specific absorption rate (SAR), a mandatory parameter for practical applications in hyperthermia.

Exploring the Antioxidant and Bioinsecticidal Activity of Spontaneous Flora Vegetal Extracts for Plant Protection and Prevention of Soil Contamination

The purpose of this article was to evaluate the application of different plant extracts with bioinsecticidal action and antioxidant activity for plants and soil protection, by substitution of the application of synthetically formulated pesticides with eco-friendly compounds. In this framework, this research focused on the utilization of plant extracts from the spontaneous flora of Moldova (Romania) as bioinsecticides for the control of field pests of the species Leptinotarsa decemlineata and their antioxidant activity. Plant extracts of oregano (Origanum vulgare), yarrow (Achillea millefolium), wormwood (Artemisia absinthium), and cowslip (Primula veris) were assessed for their antioxidant activity by the microplate spectrophotometric-based method (for polyphenols and flavonoids content and for 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenger activity) and were characterized by FTIR spectroscopy spectra. To evaluate the bioinsecticidal properties of the plant extracts, the mortality (%) and neuroleptic manifestations appearing in the middle of the monitoring period for larvae and adults of the Leptinotarsa decemlineata species were identified. Mortality (%) was statistically analyzed using a one-way analysis of variance (ANOVA) and the resulting experimental results were compared with the LSD-Fisher’s test (p &lt; 0.05). The highest mortality (%) was observed after 24 h of treatment with extracts of Origanum vulgare at 100% concentration, while the maximum effect was recorded after 48 h for Origanum vulgare at 60% and 100% concentrations.

Effect of plant extracts against Alternaria tenuissima (Kunze) Wiltshire in vitro

Goal. To investigate the fungistatic effect of plant extracts on Alternaria tenuissima in vitro.&#x0D; Methods. The study was conducted in the Laboratory of Phytopathology of the Institute of Plant Protection of NAAS (IPP NAAS) and the Laboratory of Ecology and Pharmacognosy of Research Station of Medicinal Plants of the Institute of Agroecology and Environmental Management NAAS (RSMP IAEM NAAS). Medicinal plant raw materials used in the research were grown and selected at the research sites of DSLR IAP NAAS. Plant extracts were made on its basis. Determination of the activity of plant extracts on the growth of Alternaria tenuissima culture was performed in the laboratory of phytopathology of the IPP NAAS. Agar-disk diffusion method was used. The radial growth rate and the percentage of growth inhibition of colonies were determined.&#x0D; Results. On the 5th day after the start of the experiment, all studied extracts formed colonies of significantly smaller size compared to the control. On the 7th day, extracts of sage, thyme, annual wormwood, wormwood, echinacea root, and plume poppy significantly inhibited the development of Alternaria tenuissima colonies. On the 10th day, a significant reduction in the growth of colonies of the pathogen occurred with the use of extracts of sage, annual wormwood, echinacea roots and plume poppy. Inhibition of colony growth was highest for sage, annual wormwood and plume poppy and ranged from 84.3—99.5% on day 5 to 38.1—73.4% on day 10 after inoculation.&#x0D; Conclusions. According to our results, extracts of sage (Salvia officinalis L.), annual wormwood (Artemisia annua L.) and plume poppy (Macleaya cordata L.) showed a pronounced fungistatic effect against Alternaria tenuissima. These data suggest that extracts of these plants can be used in the future to develop plant protection products.

Antioxidant Activities and Phenolic Compounds of Extract from Hallasan Wormwood

Background: Many Artemisia species are used in traditional medicine to treat gastrointestinal disorders and various other ailments. However, the antioxidant and phenolic compounds present in Hallasan wormwood (Artemisia japonica var. hallaisanensis) have not been investigated yet.<BR>Methods and Results: Extracts of Hallasan wormwood (aerial parts and roots) were extracted using methanol and sequentially treated with solvent-solvent fractionation using n-hexane, ethyl acetate, and water-saturated butanol, to yield fractions with variations in polarity. The phenolic compounds present in the extracts and fractions were determined using high performance liquid chromatography (HPLC) and colorimetry. The antioxidant activities were determined using 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging, trolox equivalent antioxidant capacity (TEAC), ferric reducing antioxidant power (FRAP), and oxygen radical antioxidant capacity (ORAC) methods. The results revealed that the ethyl acetate fraction had high total phenolic and total flavonoid contents. Furthermore, the ethyl acetate fraction of roots showed the highest antioxidant activity.<BR>Conclusions: Metanolic extracts from the roots and aerial parts of Hallasan wormwood showed different antioxidant activities; nine phenolic acids and one flavonoid were detected in Hallasan wormwood. Thus, the finding of this study provide valuable data for research on functional substances from plants.

ПРОТИВОВОСПАЛИТЕЛЬНАЯ АКТИВНОСТЬ СПРЕЯ НА ОСНОВЕ ИБУПРОФЕНА И ЖИДКОГО ЭКСТРАКТА ПОЛЫНИ

The article presents the results of determining anti-inflammatory activity of the spray based on ibuprofen and liquid wormwood extract. Anti-inflammatory activity was studied on the model of local acute carrageenan inflammation. As a result of the studies it was found that the increase in paw weight averaged 49,66% in the control group of rats, in the group of rats receiving the comparison drug «Ibuleve» - 11,26%. For the group of rats receiving combined composition and the same composition without ibuprofen, the average increase in paw weight was 7,11% and 15,07%, respectively. The index of inflammatory inhibition when using combined composition was 85,68%, the composition with liquid extract of wormwood without ibuprofen was 69,65%, the liquid extract of wormwood 1:1 was 53,00%. The index of edema suppression proves that combined composition of the spray containing ibuprofen and liquid extract of wormwood has the most pronounced anti-inflammatory effect, for which the percentage of edema suppression exceeds the value for the comparison drug on the average by 8,36%.

Research on mosquito repellent performance of bamboo-polyester blended anti-mosquito yarn seamless knitted fabric

PurposePeople have always been invaded by mosquitoes, and the development of new anti-mosquito fabrics has attracted much attention. The purpose of this paper is to study the effect of knitting process on the performance of anti-mosquito seamless fabrics and provide a basis for obtaining anti-mosquito seamless knitted fabrics with excellent comprehensive performance.Design/methodology/approachThis paper uses bamboo–polyester mosquito repellent yarn containing wormwood extract as the face of seamless knitted fabric. The test factors include ordinary material in the face yarn, ground yarn material, seamless knitted structure and arrangement ratio of ordinary yarn and anti-mosquito yarn in face yarn. According to the quasi-level additional orthogonal test, 12 knitting plans are determined, and the mosquito repellent test and durability test are performed on the fabric.FindingsThe experimental results show that the optimal fabric for anti-mosquito performance is 12#, and the average repellent rate after washing 15 times is 58.57%. The corresponding process is that the face yarn is fully anti-mosquito yarn, the fabric is a single-sided mesh structure and the ground yarn is made of 4.4tex moisture-absorbing nylon/2.2tex spandex wrapped yarn.Research limitations/implicationsIn this paper, there is still a lack of diversity in the selection of yarn materials and fabrics. In the follow-up research, the authors will use more fabrics and yarn materials for combination and experimentation and simulate and predict the mosquito resistance rate of knitted fabrics with different materials and structures.Practical implicationsThe development of anti-mosquito seamless knitted fabrics with good comprehensive performance and the use of environmentally friendly wormwood repellents not only conform to the current people's healthy and environmentally friendly life philosophy, but also promote the development of the functional seamless knitted fabric market.Social implicationsIn addition, seamless knitted fabrics have a huge market prospect, and many of their fabrics are used for sports underwear and outdoor wear. Therefore, the research and development of functional knitted fabrics will attract consumers to buy. While improving the wearing comfort, it can increase profits for the company.Originality/valueThe mosquito-proof functional seamless knitted fabric developed in this research has a high mosquito-proof rate after 15 times and can be used as underwear fabric or outdoor sports fabric.

Paracord Anklet and Bracelet Controls Against Mosquitoes

The purpose of this study is to assess the effectiveness of paracord anklet and bracelet made up of guyabano (Annona Muricata) seeds and wormwood plant (Artemisia Absinthium) extract as mosquito repellent and to determine if the paracord anklet and bracelet can be as effective as the commercial patch used in this study. In the study, 2 groups were examined: The experimental group and the control group. The experimental group with variation 1 (50 guyabano seed, 80% of water, and wormwood extract), variation 2 (75 Guyabano seed, 50% of water, and wormwood extract), and variation 3 (100 Guyabano seed, 30% of water, and wormwood extract). The control group was tested with the commercialized patch. Results showed that the overall gathered data showed very promising effectiveness, with a level of effectiveness of 6.5 means. Furthermore, the ANOVA showed no differences at all between the Control and Experimental Group (fcalc 2.20fcrit3.49, P=0.13). This may therefore mean that the paracord anklet and bracelet made up of guyabano and wormwood plant is as effective as the commercial patch used in this study. This study shows that the paracord anklet and bracelet can be considered as an alternative and environmentally friendly mosquito repellent.

Lead intoxication and beneficial effect of administering the aqueous extract of Artemisia absinthium on lipid metabolism and hypertension in male Wistar rats

Lead is a persistent and common environmental contaminant that can be found in food and water. It is well-known that lead acetate causes hyperten-sion, disturbance of lipid metabolism in humans and animals. Nowadays, the phytotherapy remains a very interesting field to recovery of deleterious effect of heavy metals. The aim of this study was to evaluate the effects of aqueous extract of Artemisia absinthium to modulating the effect of lead acetate induced changes in systolic and diastolic blood pressure levels, lipid profiles in serum, heart, aorta and liver tissues and determination of lead burden in blood, when added to intoxicated animal. Male rats (80-120) g were divided into four groups of six animals each. Intoxicated animals re-ceived intraperitoneally (i.p) injections of lead acetate (20 mg/kg/body weight) solution for 4 weeks every two days. Rats treated with plant receive orally the extract of A.absinthium (5%) for 4 weeks. Orally administration of the wormwood extract in rats intoxicated with lead acetate decreased sig-nificantly the systolic (SBP), diastolic blood pressure (DBP) and blood lead level. Levels of total lipids, TC and TG were decreased significantly in group (Pb+PL) compared to control, especially in heart and liver tissues. In serum, a significant elevation of TC and TG was noted in intoxicated rats compared to control. The cardiac damage induced by Pb was evidenced by an increase in the levels of LDH and AST. In conclusion, the data indicate that the worm-wood extract has a significant ability to reduce hypertension, blood lead lev-el, equilibrate lipid metabolism and cardiac enzymes level.

SEARCH FOR BIOLOGICALLY ACTUVE SUBSTANCES WITH ANTIMICROBIAL AND ANTIBIOTIC POTENTIATING PROPERTIES AMOUNG MEMBERS OF THE GENUS ARTEMISIA AGAINTS THE MAIN PATHOGENS OF INFECTIOUS SKIN LESIONS

Abstract. Active components of the genus Artemisia show a wide range of antimicrobial effect against most bacterial pathogens. In addition, artemisin isolated from wormwood is used to treat chloroquine-resistant malaria. Antimicrobial and antibiotic-potentiating effects of eight aqueous ethanolic extracts (40 %, 70 % and 90 %) aerial part of the genus Artemisia aqueous against S. aureus and S. epidermidis strains with various types of resistance to macrolides, lincosamides, tetracyclines and fluoroquinolones isolated from outpatients with different forms of pyoderma have been researched with agar diffusion micromethod. Determination of effective antimicrobial concentrations of antimicrobials and test extracts against staphylococci have been performed with serial dilutions micromethod. The statistical program "Statistica", computer programs UTHSCSA Image Tool 3.0 and Microsoft Office Excel 2016 have been used for statistical processing of microbiological research results. The pronounced antimicrobial effect of the wormwood extract Artemisia dracunculus L. (MIC 125.0 - 250.0 μg/ml) against all S. aureus and S. epidermidis test strains has been established. Other studied extracts showed much weaker antimicrobial effect (MIC 1000.0 - 1500.0 μg/ml). It was found that the species of staphylococci and the phenotype of resistance of test strains do not affect antimicrobial activity of the studied extracts. We have found that there is no clear сorrelation between antimicrobial properties of the studied extracts and the phenotype of resistance of staphylococci test-strains. Extracts were equally effective against staphylococcal strains with low level of resistance by efflux of antimicrobial drug and skin isolates with chromosomal resistance. The greatest resistance to BAC of the studied extracts was showed by test strains with a high level of resistance to antibiotics of MLS-group and tetracycline, exhibiting sensitivity only to tarragon wormwood.&#x0D; Active components of 70, 90 % common mugwort extract Artemisia vulgaris L. (increase of the inhibition zone up to 117 – 142 %, p&lt;0.05) and southern wormwood extract (increase of the inhibition zone up to 50 – 59 % and 74 – 122 %, respectively, p&lt;0.05) showed dose-dependent synergistic interaction with erythromycin. Common mugwort extract (70 %) showed synergistic interaction with ¼ MIC of tetracycline (increase of the inhibition zone up to 100 %) against strains with combined resistance to all studied antimicrobials. For the study we used crude total extracts of medicinal plants (40 %, 70 % and 90 % ethanol), so we expect their significantly higher antimicrobial effect against staphylococcal strains while optimizing the extraction process and subsequent purification. It should be noted that 90 % aqueous ethanol extracts showed significantly better antimicrobial properties compared to 40 % and 70 % extracts. Active compounds of tarragon wormwood Artemisia dracunculus L. extract show pronounced antimicrobial effect against S. aureus and S. epidermidis strains, the main causative agents of infectious skin lesions, with different types of resistance to macrolides, lincosamides, tetracyclines and fluoroquinolones. Dose-dependent synergistic interaction with macrolides of common mugwort Artemisia vulgaris L. extracts and southern wormwood Artemisia abrotanum L. extract with macrolides (erythromycin) and tetracycline has been revealed.