Abstract
This study focused on the purification of plant extracts, with the help of adsorption chromatography (liquid chromatography with a solid stationary phase - CLS) so that the purified plant extracts lead to obtaining bioactive compounds in a pure state or with advanced purity. The studied extracts can be divide into categories as follows: a) The purification of extracts from perilla frutescens b) The purification of extracts from rosmarinus officinalis c) The purification of extracts from artemisia annua (peliniță) For the perilla frutescens extract, dried leaves, the purification aimed at obtaining pure crystals of ursolic acid and the physico-chemical analysis of the plant extracts before and after extraction, to determine the loss of the important compounds of the plant, due to their retention in the adsorbent layer. For the rosemary and wormwood (artemisia annua) extracts, dry leaves, the purification aimed at obtaining pure crystals of bioactive compounds (ursolic acid, artemisinin) and their gravimetric dosing to determine the bioactive compound content of the original plant. Mineral adsorbents and combinations of mineral adsorbents in the form of adsorbent mixtures have been used. Glass liquid columns of various sizes were used. For each experiment, the working times (saturation-activation time, elution and column washing time) were noted. All experiments were performed with the adsorption chromatography procedure, with gravity flow column (in adsorption chromatography with gravity flow column), without using Sephadex LH-20 macroporous resins as a stationary phase.
Published Version
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