This study examined workplace drug use, emotion regulation, and perceived family income among employees in Uyo, Nigeria. Using an analytical cross-sectional design, 454 participants were purposively and randomly selected with mean age of 35.41 (SD = 8.27) years. Data from two standardized instruments—emotion regulation questionnaire and drug use disorders identification test—were analyzed by means of hierarchical regressions, independent subject t-test, Pearson correlation, and descriptive statistics. Emotion regulation correlated significantly and was a major contributing factor to workplace drug use: Employees with high level of emotion regulation reported less workplace drug use whereas those who reported more workplace drug use had low level of emotion regulation (r = −0.23; p < 0.05). However, perceived family income showed no significant difference in workplace drug use [t (452) = −0.35, p > 0.05]. A significant joint prediction was observed between emotion regulation and perceived family income on workplace drug use, F (2, 453) = 12.73, p = 0.00 level of significance. Regulation is a key determining factor to emotional disturbances and such abnormal coping strategies as drug use. Understanding the trends in workplace drug use and providing corresponding motivation and incentives as and when due is capable of maintaining and sustaining positive emotions among employees.