Working as a farmer is one type of job that has high risks, judging from the type of activity and the work environment. The work activities of horticultural farmers include preparing land for planting, caring for plants by fertilizing, pulling grass or weeds, spraying pests, and harvesting and processing or handling postharvest fruits. This is not a light job, especially if the land being worked on is quite large and there are so many plants. These cause a high workload on farmers. It is necessary to measure the workload of horticultural farmers in the agro-tourism area. Many factors affect the workload of farmers, such as internal factors and external factors. Internal factors can be influenced by aspects of work (tasks) and work organization. Meanwhile, external factors can be influenced by the working environment of farmers. This workload will also affect the work productivity of farmers. Whether these factors have a dominant effect on influencing the workload of farmers, it is considered necessary to conduct more in-depth research. This type of research includes quantitative research with a cross-sectional approach. The number of samples found with the Lemeshow formula was 33 farmers. Data collection techniques involve the distribution of questionnaires, interviews with farmers, and direct observation. The factors that affect the workload are predicted through questionnaires and direct observation. Workload categories are internal and external workloads. The data obtained in the analysis used inferential statistics, namely, factor analysis. The results of the research analysis concluded that the three components of the dominant factors affecting the workload of horticultural farmers are: (1) type of work, body mass index, and calorie intake; (2) work experience, motivation, income, work posture, lifting, and work duration; and (3) work aids, age, and work environment. It is recommended to conduct further research on things that can reduce the workload of horticultural farmers in agro-tourism areas.