Abstract Background and Objective: The role of knowledge, awareness, and practice of the principles of ergonomics in the dental profession needs to be highlighted to the dental fraternity as the prevalence and severity of musculoskeletal disorders (MSDs) are increasing in higher numbers leading to a shortened clinical life span of the dentist. Materials and Methods: A valid close-ended questionnaire was designed and circulated as a Google Form, and 200 dentists all over Gujarat consented to take part in the cross-sectional study. Data were collected and analyzed statistically to understand the relation between understanding and practice of the principles of dental ergonomics. Results: The result revealed approximately 89% of the dentists who had <5 years of clinical experience faced pain in their lower back/shoulder/arm/neck area irrespective of their gender. It was observed that the association between understanding the importance between proper illumination and magnification on a daily routine in a neutral posture was highly statistically significant with 73.8% of dentists aware and practicing it. However, we need a larger sample size to be followed for a longer time to establish and highlight the risk factors for the prevention of work-related injuries. Conclusion: As oral care needs are increasing in the Indian population at an alarming rate, the need for a competent experienced dental fraternity will be a necessity for preventive as well as treatment-based dentistry. An ergonomically established dental setup in line with the practical implementation of the principles of ergonomics and lifestyle changes will lead to a reduction in MSD.