Nowaday humanity lives in a super information world, which is marked by an increase in volume of auditory and visual information, the acceleration of change in the objective and the spirit world, increase information load in the form of electromagnetic waves caused by man. This can lead to information overload, the essence of which is that the amount of useful information coming exceed the objective possibilities of perception. The singular group, which is over loaded by information – the students. The aim of the presented work was to identify the relationship between psychophysiological indices and vegetative adaptive responses of the students from the Dnepropetrovsk National University of Oles Gonchar on information overload, which may form the basis for the formation of curricula and educational work in high school and would like to offer some prognostic model of the functional state of the students in today's environment. The study involved 120 students volunteer 2–3 courses of natural specialties Dnepropetrovsk National University of Oles Gonchar. To determine the physiological features and functional state of the students the program «Psychodiagnostics» was used. For registration of heart rate variability a heart rate monitor POLAR RS800CX was used. Testing was conducted before and after the information load. Information load carried by means of tests Schulte, Bourdon and Gorbova granted in computerized form. In boys enrolled in natural specialties found high rates of properties of neural processes. Under the influence of information load indicator latent period of reaction time choosing two out of three was significantly decreased by 15 % and the rate of functional mobility of nervous processes that characterize the strength of nervous processes significantly decreased by 17 %. Mobility and balance the nervous processes when information load decreased. These changes in performance can indicate the presence of an imbalance of the autonomic nervous system stress due to information load. Resulting in uneven tasks and the lack of a clear rhythm work. Under the influence of information load significantly increased capacity is very high-frequency range (VLF, ms2) and total power (Total, ms2). Exceeding the indicator VLF, may indicate an increased influence of humoral-metabolic mechanisms regulating heart rate. There is a tendency to increase of high-capacity and low-frequency spectra, and reduce the percentage of very low-frequency (HF) range. Also, the rate of vago-sympathetic tone (LF/HF) 2 times higher than normal, which may indicate the presence sympathicotonia. Indicators spectral analysis of heart rate variability was significantly increased, the rate of the average standard deviation (SDNN) increased by 23 % and the prevalence rate of parasympathetic regulation of sympathetic link link (pNN50) by 37 %. These changes indicate activation of the sympathetic division of the autonomic nervous system and low adaptive capacities of the autonomic nervous system. Our investigations have shown that young natural specialties of Dnepropetrovsk National University of Oles Gonchar are influenced by information load significantly deteriorated physiological parameters and heart rate variability. Infringement of autonomic adaptive responses leads to a decline in physical and mental performance of boys.