This study aims to increase the quality of work and life of food service employees and also the quality of employment of managerial subjects in the future by understanding the effects of work-life imbalance on the relation between job stress and turnover intention. Using the judgment sampling of the non-probability sampling method, this study targeted the culinary employees and food & beverage employees working for food service companies and small-size stores of food service industry in Seoul region. The results of this study could be summarized as follows. First, the work-family imbalance and the work-growth imbalance had positive(+) effects on the job stress. Thus, the time conflict, role conflict, and conflict between family members at home, would have close relations with job stress. Second, the work-family imbalance and the work-growth imbalance had positive(+) effects on the turnover intention while the job stress also had positive(+) effects on the turnover intention. In the level of organization, the turnover of competent talent causes considerable losses such as the decrease of productivity and competitiveness. The work-life balance and the decrease of job stress could be the important requirements for motivating employees and also securing/maintaining excellent talent. Third, the job stress showed the mediating effects on the relations of work-family imbalance, work-growth imbalance, and turnover intention.