Durian timber and Coconut timber grow a lot in the area of Gunung Pati Semarang, Central Java. The threat of biodeteoration in Indonesia as tropical area is quite high. Therefore, it is necessary to take protection measures in the form of preservation for prevention. The preservatives used in this study are natural preservative of soursop leaf extract. The preservation method used was cold soaking method with variation of concentration of preservative as follows: 0%, 5%, 10%, 15%, 20% and 25%. This study aims to figure out how significant the weight loss of timber and how durian wood and coconut wood’s resistance to termites based on the weight loss of timber. Result of examination of termite attack on Coconut timber with preservation of soursop leaf extract 0%, 5%, 10%, 15%, 20% and 25% obtained by weight loss of wood 17.57%, 13.43%, 10.61%, 7.67%, 5.50% and 4.20%. Meanwhile, the result of examination of termite attack on Durian timber with the concentration of soursop leaf extract 0%, 5%, 10%, 15%, 20% and 25% showed these following timber weight losses: 18.24%, 14.61%, 13.04%, 11.33%, 8.98% and 7.84%. Based on the wood resistance table, Durian timber with soursop leaf concentration of 20% and 25% were classified in the medium classification. Coconut timber with 15% and 20% soursop leaf concentrations were classified in the medium classification, while at 25% concentrations in resistant classification.