Abstract Panelized light wood frame construction is becoming more popular due to the faster construction time and shortage of onsite skilled labor. To use light wood frame panels effectively in panelized floor systems, panel-to-panel joints must be fastened adequately to allow load transfer between panels. They must also possess in-plane shear strength and stiffness comparable to stick-built, staggered-sheathed assemblies. This study was designed to develop efficient and effective panel-to-panel joints for connecting adjacent floor panels built with wood I-joists and evaluate the efficiency of the joints in achieving diaphragm action. At first, a number of these panel-to-panel joints were tested in the laboratory using a small-scale diaphragm test setup to determine their efficiency in transferring in-plane forces between panels. Test results showed that a small decrease in in-plane stiffness was expected for the most effective joints, but their strengths were significantly higher than at the same location in a conventional site-built floor diaphragm. The presence of blockings and use of two-row nailing were found to considerably improve stiffness and strength. These features can be used to mitigate the potential reduction in mechanical performance of panelized floor construction, in comparison with the site-built wood I-joist floor.