Wood Formation Research Articles

Puude hooajalise radiaalkasvu mõõtmiskuupäevaks moodustunud osa arvutusmudel Eesti tingimuste jaoks

Abstract Knowledge about the seasonal dynamics of tree growth and its relationship with environmental factors is necessary to eliminate the uncertainty due to ongoing climate change and for more precise growth modelling when re-measurements are done periodically. Despite the increasing number of studies monitoring seasonal wood formation, a considerable part of European forests, including Estonia, lacks such information. In this article, we present a date-dependent model for determining the share of seasonal radial growth for the three most common tree species in the region (Scots pine, Norway spruce and silver birch) for Estonian conditions. Since seasonal tree growth monitoring data were unavailable for Estonia, we used published seasonal radial growth data from Lithuania by Dr Adomas Vitas (2011). We tested four functions (Kumaraswamy, Weibull, Gompertz and logistic) on obtained data to approximate the seasonal development of radial growth. Kumaraswamy’s function could track the course of seasonal radial growth gains the best; thus, this function was chosen for further use. We obtained data on intra-annual radial growth from published research studies from neighbouring countries and determined the dates of growth initiation and cessation for Estonian conditions. Finally, we combined Kumaraswamy’s function and the predicted radial growth onset and cessation dates into the model that could predict the seasonal growth course and thus were able to estimate the share of newly formed increment from the dates.

The PtoTCP20-miR396d-PtoGRF15 module regulates secondary vascular development in Populus

Secondary vascular development is a key biological characteristic of woody plants and the basis of wood formation. Our understanding of gene expression regulation and dynamic changes in microRNAs (miRNAs) during secondary vascular development is still limited. Here we present an integrated analysis of the miRNA and mRNA transcriptome of six phase-specific tissues—the shoot apex, procambium, primary vascular tissue, cambium, secondary phloem, and secondary xylem—in Populus tomentosa. Several novel regulatory modules, including the PtoTCP20-miR396d-PtoGRF15 module, were identified during secondary vascular development in Populus. A series of biochemical and molecular experiments confirmed that PtoTCP20 activated transcription of the miR396d precursor gene and that miR396d targeted PtoGRF15 to downregulate its expression. Plants overexpressing miR396d (35S:miR396d) showed enhanced secondary growth and increased xylem production. Conversely, during the transition from primary to secondary vascular development, plants with downregulated PtoTCP20expression (PtoTCP20-SRDX), downregulated miR396 expression (35S:STTM396), and PtoGRF15 overexpression (35S:PtoGRF15) showed delayed secondary growth. Novel regulatory modules were identified by integrated analysis of the miRNA and mRNA transcriptome, and the regulatory role of the PtoTCP20-miR396d-PtoGRF15 signaling cascade in secondary vascular development was validated in Populus, providing information to support improvements in forest cultivation and wood properties.

The tree-ring anatomy of Robinia pseudoacacia L. As a data source for dendrogeomorphic reconstruction of past landslide movements in the landslide hot-spot of central Europe

The chronology of past landslide movements is a key source of information for effective landslide hazard determination. Dendrogeomorphological methods are currently an effective tool for obtaining chronological data but still encounter limitations in extracting landslide signals from yearly series. These limitations can be reduced by introducing anatomical analyses of the yearly series. Black locust (Robinia pseudoacacia L.), a widespread tree species occupying landslides on river banks, has been surprisingly rare in dendrogeomorphology. Thus, this study focused on the effectiveness of using both standard macroscopic signals (tree-ring eccentricity) and anatomical growth responses of this species in a dendrogeomorphic analysis of landslide movements at one of the landslide hot spots in Central Europe (the Váh River bank). Analysis of the eccentricity of tree rings from 67 individuals of R. pseudoacacia allowed us to reconstruct 13 landslide events over the last 49 years. From an anatomical point of view, a strong association was found between a significant reduction in vessel lumen area and the eccentricity of the tree rings and the tilting of the tree stems. A possible cause of the anatomical response is a combination of root damage from subsurface landslide movements and occasional tension wood formation following tree tilting. The constructed chronology confirmed the known years of landslide activity and generally complemented the known chronology. One of the results of the study is the implication for effective evaluation of the spatial extent of landslide activity, considering tree-ring-based magnitude-frequency relationships. The results of this study will thus allow further extension of the use of dendrogeomorphological methods both methodologically and biogeographically.

Wood quality of residual branches of Hymenaea courbaril L. from logging in the Amazon rainforest

Abstract Branches remain in the forest environment as logging activity residue. Considering the large size of many Amazonian trees, their branches have considerable dimensions and can contribute to a sustainable wood alternative for various applications. Due to the formation of reaction wood in the branches, relevant macro and ultrastructural changes can occur in its characteristics in relation to the trunk wood. However, the woods of the branches are not technologically well known. Therefore, this work aimed to evaluate the wood quality of branches of Hymenaea courbaril L., comparing it with wood of trunks. The extractives and lignin contents, basic density, shrinkage, fiber biometry, microfibril angle and mechanical resistance to compression parallel to the fibers were analyzed. The branch wood had smaller fiber dimensions and a higher microfibril angle than the trunk wood. The basic density was similar between these materials. The linear and volumetric shrinkages were smaller in the branch wood than in the trunk, while the axial shrinkage was higher in the branch. The parallel compressive strength was also lower in the branch wood than in the trunk. The branch wood has properties suitable for products with higher added value such as furniture, decorative objects, floors and utensils in general.

Bimodal and unimodal radial growth of Mediterranean oaks along a coast-inland gradient

Wood formation during the growing season is shaped by the intra-annual variability of climatic conditions. In the Mediterranean, the cambial activity is seasonally constrained by winter low temperature and summer drought, resulting in bimodal growth patterns. Although bimodal growth is an ecologically important adaptation of woody species to seasonally dry environments, its variability across space and among species remains unclear. We combined direct monitoring of intra-annual radial growth using dendrometers and xylogenesis micro-sampling with indirect wood formation modeling to assess growth bimodality for two coexisting oak species across six sites in North-Eastern Spain. We focused on species with two contrasting life strategies, evergreen with diffuse-porous wood (Quercus ilex) and winter deciduous with ring-porous wood (Q. faginea/Q. pubescens). Study sites spanned from oceanic areas with high autumn precipitation to dry continental inland regions. We hypothesized that growth bimodality reflects the interaction between local precipitation patterns and species-specific traits. Our results revealed cambial activity in autumn after summer quiescence across the entire region and for both oak species. However, growth bimodality and the relative contribution of the autumn growth peak to the total annual growth was geographically structured and species-specific. Growth was unimodal under continental and Atlantic conditions. By contrast, growth bimodality was significant along the Mediterranean coast, where precipitation showed a bimodal pattern. Moreover, evergreen Q. ilex showed more growth bimodality compared with deciduous Q. faginea/Q. pubescens at Mediterranean sites. Different intra-annual distribution of radial growth could facilitate coexistence of both oak species through temporal niche partitioning. The growth bimodality helps to compensate for reduced growth in summer by autumn cambial resumption and, therefore, the differences in its intensity between sites and species may determine divergent responses of forest ecosystems to ongoing climate change.

Decoupled leaf-wood phenology in two pine species from contrasting climates: Longer growing seasons do not mean more radial growth

Climate warming is expected to lengthen the growing season of tree species and enhance radial growth rates. Alternatively, a longer growing season could not lead to improved radial growth if wood production depends more on growth rate than on growing season length. We test these ideas by comparing leaf phenology data and the estimated start and end dates of wood formation predicted by the VS-Lite growth model. We analyzed long-term series of leaf unfolding and fall dates and reconstructed radial growth of two pine species under contrasting climatic conditions: Scots pine (Pinus sylvestris) in a Russian boreal site and Aleppo pine (Pinus halepensis) in a Spanish Mediterranean site. On average, leaf onset occurred in days 99 and 163 in P. halepensis and P. sylvestris, respectively, about 40 days earlier than the estimated start date of wood formation. The onset of leaf unfolding advanced 2.1 days per decade in P. sylvestris in response to warmer May temperatures. Radial growth was enhanced by warm-wet spring-summer conditions in P. sylvestris and by wet soil conditions from prior winter up to current summer in P. halepensis. In this species the growing season length and the radial growth rate were not coupled because the growing season length shortened during cool-wet periods whereas growth rates increased. In P. sylvestris leaf onset was delayed during years with low growth rates suggesting a potential coupling between warmer spring conditions, earlier leaf onset and enhanced growth whenever soil water content is high enough. Overall, we show that longer growing seasons do not necessarily imply higher radial growth rates.

High-resolution mapping reveals hotspots and sex-biased recombination in Populus trichocarpa.

Fine-scale meiotic recombination is fundamental to the outcome of natural and artificial selection. Here, dense genetic mapping and haplotype reconstruction were used to estimate recombination for a full factorial Populus trichocarpa cross of 7 males and 7 females. Genomes of the resulting 49 full-sib families (N = 829 offspring) were resequenced, and high-fidelity biallelic SNP/INDELs and pedigree information were used to ascertain allelic phase and impute progeny genotypes to recover gametic haplotypes. The 14 parental genetic maps contained 1,820 SNP/INDELs on average that covered 376.7 Mb of physical length across 19 chromosomes. Comparison of parental and progeny haplotypes allowed fine-scale demarcation of cross-over regions, where 38,846 cross-over events in 1,658 gametes were observed. Cross-over events were positively associated with gene density and negatively associated with GC content and long-terminal repeats. One of the most striking findings was higher rates of cross-overs in males in 8 out of 19 chromosomes. Regions with elevated male cross-over rates had lower gene density and GC content than windows showing no sex bias. High-resolution analysis identified 67 candidate cross-over hotspots spread throughout the genome. DNA sequence motifs enriched in these regions showed striking similarity to those of maize, Arabidopsis, and wheat. These findings, and recombination estimates, will be useful for ongoing efforts to accelerate domestication of this and other biomass feedstocks, as well as future studies investigating broader questions related to evolutionary history, perennial development, phenology, wood formation, vegetative propagation, and dioecy that cannot be studied using annual plant model systems.

The Glutathione S-Transferase PtGSTF1 Improves Biomass Production and Salt Tolerance through Regulating Xylem Cell Proliferation, Ion Homeostasis and Reactive Oxygen Species Scavenging in Poplar.

Glutathione S-transferases (GSTs) play an essential role in plant cell detoxification and secondary metabolism. However, their accurate functions in the growth and response to abiotic stress in woody plants are still largely unknown. In this work, a Phi class Glutathione S-transferase encoding gene PtGSTF1 was isolated from poplar (P. trichocarpa), and its biological functions in the regulation of biomass production and salt tolerance were investigated in transgenic poplar. PtGSTF1 was ubiquitously expressed in various tissues and organs, with a predominant expression in leaves and inducible expression by salt stress. Transgenic poplar overexpressing PtGSTF1 showed improved shoot growth, wood formation and improved salt tolerance, consistent with the increased xylem cell number and size under normal condition, and the optimized Na+ and K+ homeostasis and strengthened reactive oxygen species scavenging during salt stress. Further transcriptome analyses demonstrated that the expressions of genes related to hydrolase, cell wall modification, ion homeostasis and ROS scavenging were up- or down-regulated in transgenic plants. Our findings imply that PtGSTF1 improves both biomass production and salt tolerance through regulating hydrolase activity, cell wall modification, ion homeostasis and ROS scavenging in transgenic poplar, and that it can be considered as a useful gene candidate for the genetic breeding of new tree varieties with improved growth under salt stress conditions.

Anatomical, physiological, biochemical and molecular responses of Eucalyptus spp. under water deficit conditions and characteristics of Tunisian arid species: an overview

The genus Eucalyptus occurs in a large range of environmental conditions, mainly arid/semi-arid areas. It includes species with high capacity to survive with extremely low water potential. For that, our review shows an important effect of drought on Eucalyptus spp. plants’ resistance mechanisms and management strategies. In fact, water stress acts directly on growth, productivity, yield, it affects also response to pests and diseases, disturbs wood formation and essential oil yield. However, the general patterns of response to water stress varied among species, genotypes, hybrids and clones. To assume, reducing water loss in eucalyptus species is manifested by reducing leaves area, reducing gas exchange, increasing water uptake thanks to a prolific and deep root systems. A greater accumulation of osmolytes that gives rise to osmotic adjustment including carbohydrates especially cyclitols and quercitol, other amino acids and organic acids, also some proteins which play a vital role in sustaining cellular functions under drought conditions. More than that, water stress increases mainly the levels of pigments, chlorophyll fluorescence parameters, malondialdehyde (MDA), abscisic acid (ABA) and the biosynthesis of triacylglycerols (TAGs) in Eucalyptus species.

Spatiotemporal variation and covariation of heartwood color in planted teak wood from Ghana

Heartwood color is a complex trait that affects the economic and aesthetic value of the wood but is highly variable. How the color of the heartwood varies spatially and temporally is poorly understood. To illustrate how heartwood color varies within a tree, two opposite aspects of wood within the same tree, representing differential growth rate, were used to model the long-short axis system jointly. The color of the heartwood on the long and the short axis was considered to be two different traits. By jointly modeling the long and short axes, the correlation was examined between aspect (spatial) and contemporaneous correlations (within aspect). Spatial and temporal correlations and their interactions describe the indirect physiological, genetic, and environmental changes in wood formation with time and position in the trunk. Spatial correlations were consistently lower than temporal correlations but were positive and significant. Between the heartwood color parameters, b* showed a relatively higher spatial correlation. The results suggest that there is a spatial correlation in the long-short axis for all color parameters and in the two surfaces. Variations between aspects were not statistically significant for any color parameter. The bivariate mixed model method revealed hidden physics behind heartwood color formation. Models need to be developed to account for both spatial and temporal dependence in studies of wood property change.

Intra-Annual Growth and Its Response to Climatic Factors of Two Salix Species under Warm Temperate Environment

Monitoring cambial activity and intra-annual growth dynamics is an effective method for identifying tree growth response to climate change. However, there have been few pieces of research on intra-annual wood formation of diffuse-porous species under monsoonal warm temperate environment. Here, we monitored weekly the cambial activity and xylem differentiation of Salix babylonica and Salix matsudana by collecting microcores during the 2018 growing season. Two willow species exhibited similar cambial activity and xylem differentiation processes, of which the onset and cessation of xylem growth was from middle/late March to early/middle November. The onset and cessation of cambial activity were slightly earlier for Salix matsudana (19 March and 12 October) than for Salix babylonica (26 March and 17 October), which peaked on 2 June and 31 May, respectively. Salix babylonica showed wider xylem increment and higher growth rate than that of Salix matsudana, of which the intra-annual xylem width was 8525 ± 1201 µm and 7603 ± 826 µm, respectively, fitted by Gompertz function. Moreover, the maximum growth rate of Salix babylonica and Salix matsudana was 79.75 μm day−1 and 66 μm day−1, respectively, occurring on 4 June (DOY155) and 26 May (DOY146). Both temperature and water availability were important factors influenced the xylem growth for two species, which Salix matsudana had a stronger response to temperature but not to water availability than Salix babylonica. These results suggested that Salix babylonica seem to grow better under moist warm temperate environment due to it being a more conservative response to the climate than Salix babylonica. These observed species-specific differences at the intra-annual scale may help researchers more accurately anticipate the species suitability in temperate forests.

Wood from Forest Residues: Technological Properties and Potential Uses of Branches of Three Species from Brazilian Amazon

Branch wood presents potential volumetry that may have several applications, and its use may improve forest management efficiency in the Amazon. However, there is a lack of knowledge regarding the properties of branch wood when compared to the stem or what its potential applications might be, considering the possible variability of wood formation in branches. This study aimed to characterize physically and mechanically the branch wood of Dipteryx odorata, Hymenolobium petraeum and Hymenaea courbaril and to compare them with their respective stems. No significant statistical differences were observed for the basic density between the branch and stem woods. The branch wood of Dipteryx odorata and Hymenaea courbaril showed a lower coefficient of anisotropy (1.23 and 1.99, respectively) than the stem wood (1.62 and 2.49, respectively). D. odorata showed similar mechanical properties between the branch and stem, except for hardness, while H. petraeum and H. courbaril branch wood showed lower strengths when compared to the stem wood for all mechanical tests, except for the shear strength test. Branch wood has similar potential uses to stem wood and can be used for non-structural purposes such as small artifacts, decorative items, furniture, tools and panels composed of short, glued pieces.

Aspartic proteases modulate programmed cell death and secondary cell wall synthesis during wood formation in poplar.

Programmed cell death (PCD) is essential for wood development in trees. However, the determination of crucial factors involved in xylem PCD of wood development is still lacking. Here, two Populus trichocarpa typical aspartic protease (AP) genes, AP17 and AP45, modulate xylem maturation, especially fibre PCD, during wood formation. AP17 and AP45 were dominantly expressed in the fibres of secondary xylem, as suggested by GUS expression in APpro::GUS transgenic plants. Cas9/gRNA-induced AP17 or AP45 mutants delayed secondary xylem fibre PCD, and ap17ap45 double mutants showed more serious defects. Conversely, AP17 overexpression caused premature PCD in secondary xylem fibres, indicating a positive modulation in wood fibre PCD. Loss of AP17 and AP45 did not alter wood fibre wall thickness, whereas the ap17ap45 mutants showed a low lignin content in wood. However, AP17 overexpression led to a significant decrease in wood fibre wall thickness and lignin content, revealing the involvement in secondary cell wall synthesis during wood formation. In addition, the ap17ap45 mutant and AP17 overexpression plants resulted in a significant increase in saccharification yield in wood. Overall, AP17 and AP45 are crucial modulators in xylem maturation during wood development, providing potential candidate genes for engineering lignocellulosic wood for biofuel utilization.

Upscaling xylem phenology: sample size matters.

Upscaling carbon allocation requires knowledge of the variability at the scales at which data are collected and applied. Trees exhibit different growth rates and timings of wood formation. However, the factors explaining these differences remain undetermined, making samplings and estimations of the growth dynamics a complicated task, habitually based on technical rather than statistical reasons. This study explored the variability in xylem phenology among 159 balsam firs [Abies balsamea (L.) Mill.]. Wood microcores were collected weekly from April to October 2018 in a natural stand in Quebec, Canada, to detect cambial activity and wood formation timings. We tested spatial autocorrelation, tree size and cell production rates as explanatory variables of xylem phenology. We assessed sample size and margin of error for wood phenology assessment at different confidence levels. Xylem formation lasted between 40 and 110 d, producing between 12 and 93 cells. No effect of spatial proximity or size of individuals was detected on the timings of xylem phenology. Trees with larger cell production rates showed a longer growing season, starting xylem differentiation earlier and ending later. A sample size of 23 trees produced estimates of xylem phenology at a confidence level of 95 % with a margin of error of 1 week. This study highlighted the high variability in the timings of wood formation among trees within an area of 1 km2. The correlation between the number of new xylem cells and the growing season length suggests a close connection between the processes of wood formation and carbon sequestration. However, the causes of the observed differences in xylem phenology remain partially unresolved. We point out the need to carefully consider sample size when assessing xylem phenology to explore the reasons underlying this variability and to allow reliable upscaling of carbon allocation in forests.

PagDET2 promotes cambium cell division and xylem differentiation in poplar stem.

Secondary growth of the woody tree stem is governed by meristematic cell division and differentiation in the vascular cambium. Multiple hormonal signals and endogenous developmental programs regulate vascular cambium activity. Brassinosteroids (BRs) significantly promote secondary stem growth and wood formation in poplar trees. However, the underlying regulatory mechanisms of BRs within the vascular tissue remain unclear. Genetic and anatomical approaches were used here to elucidate the role of PagDET2, the rate-limiting enzyme for BRs biosynthesis, in regulating secondary vascular cambium activity in Populus. This study showed that the elevated endogenous castasterone (CS) levels in tree stems through overexpressing PagDET2 could enhance cambium meristem cell activity and xylem (XY) differentiation to promote secondary stem growth. RNA-seq analysis revealed that genes involved in BRs response, vascular cambium cell division, XY differentiation, and secondary cell wall synthesis were up-regulated.

Insights into source/sink controls on wood formation and photosynthesis from a stem chilling experiment in mature red maple.

Whether sources or sinks control wood growth remains debated with a paucity of evidence from mature trees in natural settings. Here, we altered carbon supply rate in stems of mature red maples (Acer rubrum) within the growing season by restricting phloem transport using stem chilling; thereby increasing carbon supply above and decreasing carbon supply below the restrictions, respectively. Chilling successfully altered nonstructural carbon (NSC) concentrations in the phloem without detectable repercussions on bulk NSC in stems and roots. Ring width responded strongly to local variations in carbon supply with up to seven-fold differences along the stem of chilled trees; however, concurrent changes in the structural carbon were inconclusive at high carbon supply due to large local variability of wood growth. Above chilling-induced bottlenecks, we also observed higher leaf NSC concentrations, reduced photosynthetic capacity, and earlier leaf coloration and fall. Our results indicate that the cambial sink is affected by carbon supply, but within-tree feedbacks can downregulate source activity, when carbon supply exceeds demand. Such feedbacks have only been hypothesized in mature trees. Consequently, these findings constitute an important advance in understanding source-sink dynamics, suggesting that mature red maples operate close to both source- and sink-limitation in the early growing season.

What drives forest carbon storage? The ramifications of source-sink decoupling.

This article is a Commentary on Martínez‐Sancho et al. (2022), 236: 58–70.

Genetic architecture behind developmental and seasonal control of tree growth and wood properties in Norway spruce.

Genetic control of tree growth and wood formation varies depending on the age of the tree and the time of the year. Single-locus, multi-locus, and multi-trait genome-wide association studies (GWAS) were conducted on 34 growth and wood property traits in 1,303 Norway spruce individuals using exome capture to cover ~130K single-nucleotide polymorphisms (SNPs). GWAS identified associations to the different wood traits in a total of 85 gene models, and several of these were validated in a progenitor population. A multi-locus GWAS model identified more SNPs associated with the studied traits than single-locus or multivariate models. Changes in tree age and annual season influenced the genetic architecture of growth and wood properties in unique ways, manifested by non-overlapping SNP loci. In addition to completely novel candidate genes, SNPs were located in genes previously associated with wood formation, such as cellulose synthases and a NAC transcription factor, but that have not been earlier linked to seasonal or age-dependent regulation of wood properties. Interestingly, SNPs associated with the width of the year rings were identified in homologs of Arabidopsis thaliana BARELY ANY MERISTEM 1 and rice BIG GRAIN 1, which have been previously shown to control cell division and biomass production. The results provide tools for future Norway spruce breeding and functional studies.

Biomass formation and sugar release efficiency of Populus modified by altered expression of a NAC transcription factor.

Woody biomass is an important feedstock for biofuel production. Manipulation of wood properties that enable efficient conversion of biomass to biofuel reduces cost of biofuel production. Wood cell wall composition is regulated at several levels that involve expression of transcription factors such as wood‐/secondary cell wall‐associated NAC domains (WND or SND). In Arabidopsis thaliana , SND1 regulates cell wall composition through activation of its down‐stream targets such as MYBs. The functional aspects of SND1 homologs in the woody Populus have been studied through transgenic manipulation. In this study, we investigated the role of PdWND1B, Populus SND1 sequence ortholog, in wood formation using transgenic manipulation through over‐expression or silencing under the control of a vascular‐specific 4‐coumarate‐CoA ligase (4CL) promoter. As compared with control plants, PdWND1B‐RNAi plants were shorter in height, with significantly reduced stem diameter and dry biomass, whereas there were no significant differences in growth and productivity of PdWND1B over‐expression plants. Conversely, PdWND1B over‐expression lines showed a significant reduction in cellulose and increase in lignin content, whereas there was no significant impact on lignin content of downregulated lines. Stem carbohydrate composition analysis revealed a decrease in glucose, mannose, arabinose, and galactose, but an increase in xylose in the over‐expression lines. Transcriptome analysis revealed upregulation of several downstream transcription factors and secondary cell wall related structural genes in the PdWND1B over‐expression lines, partly explaining the observed phenotypic changes in cell wall chemistry. Relative to the control, glucose release efficiency and ethanol production from stem biomass was significantly reduced in over‐expression lines. Our results show that PdWND1B is an important factor determining biomass productivity, cell wall chemistry and its conversion to biofuels in Populus.

Induced Wood-Inorganic Composites in Standing Trees via Slow-Release Drip

It is a novel idea to fabricate wood-inorganic composites by utilizing the transpiration of bionic trees to realize the self-assembly of inorganic precursors in wood formation. We selected a 10-year-old poplar and diffused the solvent or sol containing SiO2 precursor into the xylem via the slow-release drip method. In combination with the moisture in xylem, reactions such as hydrolysis, polycondensation and self-assembly were induced in order to form wood inorganic composites. It was found, through microscopic observation, that such inorganic substances were yellowish brown and widely existed in vessels, wood fibers and ray cells. For the new grown wood, the fiber–tissue ratio and cell wall thickness underwent an increase, while the vessel diameter and tissue ratio experienced a decline. Moreover, such change was related to the concentration of precursors. EDS analysis proved that the elemental composition of sediments in wood cells was C, O, Si, K and Ca. XPS confirmed that the newly formed wood contained silicon oxide, illustrating that the standing tree slow-release drip technology could induce wood to fabricate inorganic composites.