Wood Extractives Research Articles

Angular dependence of random laser emission by using ZnO-CuO heterostructure as scatterer and its applications in biocompatible imaging.

The angular dependence of random laser (RL) generation in a commercially available rhodamine 6G (Rh6G) dye has been demonstrated using ZnO-CuO heterostructure as passive scatterers. The grass-like superstructure formed at a 1M:1M molar ratio of ZnO-CuO significantly enhances scattering, resulting in RL spikes with a full width at half maximum (FWHM) of just a few nanometer and a noticeable reduction in the RL threshold. RL emission spectra were collected over an angular spread of 0-180 degrees, revealing a remarkable shift in RL emission from 566nm to 580nm. Additionally, we explored RL using a biological extract from Caesalpinia sappan L (Biancaeasappan L) and demonstrated speckle-free imaging with an affordable handheld microscope called the Foldscope. The biocompatibility of the wood extract shows significant potential for applications in speckle-free imaging. We had assessed the Pearson correlation coefficient between images captured under LED, RL, and conventional laser illumination. It is observed that the value of the coefficient becomes the lowest under RL illumination which confirms the formation of high-resolution and artifact-free images.

Nanoparticle Synthesis of Secang Wood Extract (Caesalpinia sappan L.) as Antibacterial Agent

Nanoparticle Synthesis of Secang Wood Extract (Caesalpinia sappan L.) as Antibacterial Agent

Metabolic profiling and antibacterial activity of tree wood extracts obtained under variable extraction conditions

IntroductionTree bacterial diseases are a threat in forestry due to their increasing incidence and severity. Understanding tree defence mechanisms requires evaluating metabolic changes arising during infection. Metabolite extraction affects the chemical diversity of the samples and, therefore, the biological relevance of the data. Metabolite extraction has been standardized for several biological models. However, little information is available regarding how it influences wood extract’s chemical diversity.ObjectivesThis study aimed to develop a methodological approach to obtain extracts from different tree species with the highest reproducibility and chemical diversity possible, to ensure proper coverage of the trees’ metabolome.MethodsA full factorial design was used to evaluate the effect of solvent type, extraction temperature and number of extraction cycles on the metabolic profile, chemical diversity and antibacterial activity of four tree species.ResultsSolvent, temperature and their interaction significantly affected the extracts’ chemical diversity, while the number of extraction cycles positively correlated with yield and antibacterial activity. Although 60% of the features were recovered in all the tested conditions, differences in the presence and abundance of specific chemical classes per tree were observed, including organooxygen compounds, prenol lipids, carboxylic acids, and flavonoids.ConclusionsEach tree species has a unique metabolic profile, which means that no single protocol is universally effective. Extraction at 50 °C for three cycles using 80% methanol or chloroform/methanol/water showed the best results and is suggested for studying wood metabolome. These observations highlight the need to tailor extraction protocols to each tree species to ensure comprehensive metabolome coverage for metabolic profiling.

Efficient Separation of Poplar Lignin Using a New Carboxylic Acid-Based Deep Eutectic Solvents - Choline Chloride/Malonic Acid.

Separation of lignin by pretreatment is an important step in biomass refining. This study investigated how a novel dicarboxylic acid-based deep eutectic solvent (DES) - choline chloride (ChCl)/malonic acid (MA) - affected the process of separating lignin from poplar. At 140 °C for 3.0 h, with a ChCl: MA molar ratio of 1: 3.5, the ideal pretreatment conditions were met, and 91.8 % lignin was obtained. Even after five DES reuses, the consistent and effective separation efficiency of 77.9 % remains unchanged. The hydrolysate contained 92.4 % of the recovered lignin, with a purity of 94.6 %. Moreover, the regenerated lignin obtained through the new DES pretreatment exhibited a high phenolic hydroxyl content of 1.9 mmol g-1 and a low polydispersity index of 1.4. The results showed efficient and selective separation of lignin using the new binary carboxylic acid-based DES pretreatment was achieved. This research offers a novel approach to effectively separate wood fiber biomass and extract valuable lignin.

Antimicrobial activities of ethanolic Kiam (Cotyleobium lanceotatum) wood extract and its application to extend shelf life of refrigerated Pacific white shrimp

Antimicrobial activities of ethanolic Kiam (Cotyleobium lanceotatum) wood extract and its application to extend shelf life of refrigerated Pacific white shrimp

HISTOPATHOLOGICAL STRUCTURE OF THE RAT KIDNEY AFTER ADMINISTRATION OF SAPPAN WOOD EXTRACT (Caesalpinia sappan L.) IN IRON OVERLOAD CONDITION

Excessive amounts of iron in the body can lead to damage to various organs, including the kidneys. Iron chelators have been demonstrated to effectively reduce the accumulation of this excess iron. This study aims to investigate the impact of administering sappan wood extract (Caesalpinia sappan) as an adjuvant to iron chelators on the structure and function of kidneys in a rat model of iron overload. The experimental research, spanning 28 days, employed a Completely Randomized Design (CRD) involving 30 male Wistar rats (Rattus norvegicus) distributed across 6 test groups. Iron dextran at 60 mg/kg bb induced iron overload, while a comparative iron chelator, deferiprone, was given at 1.35 mg/kg bb. Various doses of Sappan wood extract (SWE) 100, 150, and 200 mg/kg bb were administered to separate test groups. The parameters that are being observed include the distribution of iron and the structure of kidney injury. At a 95% confidence level, the acquired data were examined using analysis of variance (ANOVA), and the Duncan test was used to see whether there were any differences. The study's findings demonstrated that each treatment group's kidney iron levels differed from the excess iron-containing control group (p <0.05). The histological investigation results demonstrated a significant difference in damage scores (p <0.05) between the groups administered secang wood extract and the excess besei control group. Adminstered of 100 mg/kgbw dose of EKS might lower their organ iron levels and lessen the harm that the iron did to their kidneys. Keyword: Sappan Wood (Caesalpinia sappan L.), Iron Overload, Kidney

Analysis of Balanites aegyptiaca wood extractives and their variation across different ecological zones

This study focuses on the analysis of extractives from the wood of Balanites aegyptiaca, an endemic and iconic species of dry African regions, and the impact of climatic and geographical conditions on these extractives. The wood samples were collected from three distinct geographical zones: the Sahelian Chadian zone, the Sudanian Chadian zone, and the Sahelian Senegalese zone. A total of nine trees were analyzed in this study. The results showed that samples from the Sahelian Chadian zone, characterized by more arid conditions, had a significantly higher extractive content compared to those from the other two regions. The extractive compounds characteristic of these samples included stereoisomers of inositol, pinnitol, diosgenin, and sesquiterpenes. These compounds were found to be identical in samples from the Sahelian Senegalese and Sudanian Chadian zones, except for disaccharides, which were additionally present in the Senegalese samples. For the same species, pinnitol remained the most dominant compound in the wood from the Sahelian Chadian zone, alongside monosaccharides. This marked difference in extractive compounds is explained as a response of the tree to local constraints, particularly water stress and high temperatures, which stimulate the production of secondary metabolites such as tannins, polysaccharides, and pinnitol. These compounds are essential for the tree's resistance to unfavorable environmental conditions. The study highlights the influence of climatic factors on the biosynthesis of secondary compounds and suggests that Balanites aegyptiaca could offer promising opportunities for pharmacological and industrial applications.

Brazilin-rich extract from Sappan wood attenuates neuroinflammation through the restoration of the gut microbiota: improving cognitive function in mice with age-related Alzheimer’s disease

Alzheimer’s disease is an age-related neurodegenerative disorder characterized by amyloid-β accumulation, which leads to memory impairment, neuroinflammation, and imbalance in the gut microbiota. Targeting neuroinflammation and correcting the imbalance in the gut microbiota may be promising strategies for treating age-related Alzheimer’s disease. Sappan wood, a traditional Chinese medicine known for its neuroprotective and anti-inflammatory properties, has not been extensively studied for its effects on cognitive function in age-related Alzheimer’s disease and neuroinflammation. In this study, we demonstrated that Sappan wood extract, brazilin, is nontoxic and reduces the degree of cytotoxicity induced by amyloid-β oligomers in PC12 cells. Sappan wood extract can alleviate cognitive deficits and protect hippocampal neurons in the mouse model of age-related Alzheimer’s disease by reducing neuroinflammation caused by amyloid-β42 oligomers. Furthermore, Sappan wood extract significantly reverses the imbalance in the gut microbiota caused by amyloid-β oligomers, enhancing resistance to inflammatory damage and age-related Alzheimer’s disease. Therefore, Sappan wood extract shows significant potential for combating age-related Alzheimer’s disease by improving cognitive impairment and inhibiting neuroinflammation through the modulation of the gut microbiota.

Tracing the geographic origin of wood in pulp and paper by GC-MS analysis of extractives

Abstract This study explores the application of chemotaxonomic methods to trace the geographic origin of wood used in paper production. Utilizing thermal desorption gas chromatography-mass spectrometry (TD-GC-MS) and principal component analysis (PCA), distinct chemical profiles were identified in both hydrophobic and hydrophilic extracts. Residual wood extractives, that are capable of origin differentiation, were found in acetone extracts of the CITES-protected genus ramin (Gonystylus Teijsm. et Binn. spp.), petroleum ether extracts of red meranti (Rubroshorea (Meijer) P.S.Ashton et J.Heck. spp.) and n-hexane extracts of teak (Tectona grandis L.f.) pulp. The PCA results demonstrate the potential of these chemical fingerprints to serve as reliable indicators of wood provenance, which could potentially be crucial for the practical execution of the European Deforestation Regulation (EUDR) and the US Lacey Act in the paper industry. Despite the promising findings for ramin and teak, the effectiveness of the method to distinguish the origin of red meranti pulps was limited in some cases.

Toxicological Evaluation of Ginger (Zingiber afficinale) and Kayu Secang (Biancaea sappan) Extracts using Brine Shrimp (Artemia Salina L.)

Ginger (Zingiber oficinale) and sappan wood (Biancaeae sappan) are herbaceous plants that are commonly used as herbal decoctions. Due to their wide biological and pharmacological activity, many natural compounds can serve as the foundation for the creation of modern medicines. Some of them are also known to have toxicological characteristics that are important in the development of cancer drugs. Objective: This study aims to evaluate the toxic effects of secang wood extract (Biancaeae sappan) and ginger (Zingiber oficinale) on saltwater shrimp (Artemia salina L.).Methods: The design of this study was a laboratory experimental analysis using the Brine Shrimp (Artemia Salina L.) method. The extract was prepared using standard methods and its toxicity was evaluated using a saltwater shrimp death test. Results: The results of the study were shown by determining its lethal concentration (LC50) which affects 50% of the shrimp population. showed that ginger extract was very toxic to shrimp brine with an LC50 value of 12.91. This shows that both extracts have high toxicity and can have a good effect on cells undergoing carcinogenesis even at relatively low concentrations. Conclusion: These findings highlight the importance of careful use and proper handling of extracts to minimize the potential health risks associated with the toxicity of herbal extracts. Further research is needed to elucidate the specific components responsible for the observed toxicity and to explore the potential use of herbal extracts as anti-cancer and antioxidants.

Effect of Keruing and Meranti Wood Extracts on the Nail Corrosion Rate

Effect of Keruing and Meranti Wood Extracts on the Nail Corrosion Rate

Differences in Inhibitory Effectiveness of Salam Leaf Extract (Syzygium Polyanthtalum) and Secang Wood Extract (Caesalpinia sappan L.) Against the Growth of Escherichia Coli Bacteria

Bay leaves (Syzygium polyanthum) and a second wood (Caesalpinia sappan L) are widely known to the public as natural antiseptics for prevention and treatment, including treating digesting diseases, one of which is diarrhea caused by Escherichia coli. Bay leaves and Secang wood can be used as antiseptics because they contain the active substance tannin. This research aims to determine the difference in the antibacterial effectiveness of 96% ethanol extract of bay leaves (Syzygium polyanthum) and 96% ethanol extract of second wood (Caesalpinia sappan L) on growth Escherichia coli bacteria. The type of research is experimental. The method used in this research is the Kirby-Bauer diffusion method. This method observes the clear zone produced from a blank disk that has been treated with bay leaf extract and second wood extract at a concentration of 30%, 60%, and 90% for incubation at 37?C for 24 hours. From the research results, it can be concluded that the ethanol extract of bay leaves with a concentration of 30%, 60%, and 90% with an inhibition zone diameter of 0 mm, 7,6 mm, and 9,6 mm. The second wood at a concentration of 30%, 60%, and 90% with an inhibition zone diameter of 10 mm, 11,3 mm, and 14 mm. The statistical test shows that the data is normally distributed with a parametric test ANOVA, p =0,00, which means that there is no significant difference in the diameter of the inhibition zone between the ethanol extract of bay leaves and the ethanol extract of second wood.

Karakteristik Fisik dan Kimia Edible Film Maizena dengan Penambahan Ekstrak Kayu Secang (Caesalpinia Sappan L.)

The purpose of this research was to obtain the concentration of secang wood extract that produces the best physcochemical characteristics of maizena edible fim. This study used randomized block design (RBN) with one factor and five replications. The factor of this study was the addition of secang wood extract into maizena edible film which consisted of five levels, namely s0 (0%); s1 (1%); s2 (3%); s3 (5%); and s4 (7%). Analysis of physical and chemical characteristics include thickness, solubility, water holding capacity, antioxidant activity, and total flavonoid compound. The best edible film was selected by De Garmo test. The result showed that 7% of secang wood concentration made the best physcochemical characteristics of maizena edible film with 0,154 mm of thickness, 43,253% of solubility, 4,111 (g/g) of Water Holding Capacity (WHC), 96,485% of antioxidant activity, 252,727 mg QE/g extract of total flavonoid compound.

Antibacterial Effectiveness Test of South Kalimantan Bajakah Wood Extract against the Bacteria Staphylococcus Aureus Atcc 25923 Article Sidebar

Introduction: Root canal treatment is a treatment that removes necrotic pulp tissue, biofilm and debris from the root canal system so that it can trigger the formation of healthy periapical tissue. Staphylococcus aureus is one of the important resistant microorganisms that is often isolated from root canal treatment. Bajakah wood (Spatholobus littoralis Hassk) is known to contain flavonoids, saponins, tannins and polyphenols. Material and Methods: This research uses the well method. The number of samples was 20 samples with Bajakah wood extract as the test material. This research uses a posttest only control group design. Results and Discussions: Data analysis using the Kruskal-Wallis test showed that there were significant differences (p<0.05) between the treatment groups. Conclusion: There is no resistance to South Kalimantan Bajakah wood extract in concentrations of 50%, 75%, 100% against Staphylococcus aureus bacteria, which means there is no antibacterial activity.

Investigating the potential of waste oyster shell as a sustainable bio-mordant in natural dyeing

The use of natural bioresources in textile dyeing has attracted significant research interest due to their environmentally friendly and low-toxic nature. This study investigated the utilization of waste oyster shell (WOS) as a bio-mordant combined with sappan wood extract to dye silk fabrics using pre-, meta-, and post-mordanting method. The crystal structure of CaCO3 derived from WOS and their impact on color properties, color strength and color fastness were analyzed. The dyed fabrics displayed a range of shades from light pink to deep red and brown. FTIR analysis confirmed chemical interactions among dye, silk, and WOS mordants. Notably, silk treated with WOS at 800 °C as a pre-mordant showed color strength approximately 2.6 times higher than that of un-mordanted samples. Additionally, bio-mordanted samples exhibited improved color fastness compared to untreated ones (rating 2), with pre-mordanting offering the highest resistance to washing (rating 4–5). These findings shed light on the potential of waste oyster shell as an effective, sustainable alternative to conventional metal mordants in textile dyeing. This study not only support the utilization of waste but also enhances environmental and resource efficiency of the dyeing process.

Multidimensional Exploration of Wood Extractives: A Review of Compositional Analysis, Decay Resistance, Light Stability, and Staining Applications

Extractives, which naturally evolve as fundamental defense mechanisms in wood against environmental stresses, hold an essential place in the field of wood conservation science. Despite their low content in woody substrates, extractives are chemically complex and can be extracted accurately by solvents with different polarities, covering key components such as aliphatic, terpenoid, and phenolic compounds. The application of solvent extraction allows for the effective recovery of these extracts from forestry waste, thereby creating new opportunities for their reuse in wood modification and enhancing the economic value and potential applications of forestry waste. In the wood industry, extractives not only act as efficient preservatives and photo-stabilizers, significantly improving the decay resistance and photodegradation resistance of wood, but also serve as ideal dyes for fast-growing wood due to their abundant natural colors, which lend the product a distinct aesthetic appeal. The aim of this paper is to provide a comprehensive review of the origin and distribution characteristics of wood extractives and to examine the impact of solvent selection on extraction efficiency. At the same time, the mechanism of extractives in enhancing wood decay resistance and slowing down photodegradation is deeply analyzed. In addition, specific examples are presented to illustrate their wide utilization in the wood industry. This is intended to provide references for research and practice in related fields.

Structural analysis of fossilized tree trunks from the Miocene Mydlovary Formation of the South Bohemian Basin (Czech Republic)

Tree trunks were found in situ in Miocene strata of the Mydlovary Formation of the South Bohemian Basin. Our study presents microscopic and chemical tissue analyses of these tree trunks. The trees were fossilized and flattened. However, they are well preserved, which made the analyses feasible. Good preservation of the tissues is indicated by the high Tissue Preservation Index, the high content of cellulose, and the slightly gelified state of the wood. The Gelification Index indicated that there was only limited alteration during diagenesis. Huminite macerals prevailed over liptinite and inertinite macerals, and two varieties of ulminite and textinite were distinguished, differing in colour reflection, light fluorescence, and reflectance values. The biomarker composition in the extracts of the fossil wood obtained by gas chromatography-mass spectrometry, included terpenoid signatures, supporting a relationship to the Family Cupressaceae s.s.. Gradual burial of the trees is indicated by the fact that they lay crosswise on top of each other. The character of the sedimentary environment and the physiological differences of the trees could explain the deformation and the high degree of preservation.

Testing the Inhibitory Activity of Bajakah Tampala (Spatholobus littoralis Hassk) Wood Extract from South Kalimantan on the Growth of Pseudomonas aeruginosa Bacteria

Background: Dental root canal treatment aims to eliminate microorganisms that cause infection in the tooth root canal, one of which is Pseudomonas aeruginosa bacteria. Disinfection of dental root canals with 0.5% - 5.25% NaOCl irrigation solution is still constrained so it is necessary to look for alternatives. The bajakah tampala plant (Spatholubus littoralis Hassk) is known to contain antibacterial compounds, namely flavonoids, saponins, tannins, and polyphenols. Objective: To evaluate the inhibitory activity of bajakah tampala wood extract (Spatholobus littoralis Hassk) from South Kalimantan against the growth of Pseudomonas aeruginosa bacteria. Method: Post test-only with control group design. The test groups were 100%, 75%, 50% bajakah tampala wood extract solution and positive control 2.5% NaOCL solution. Extraction using maceration method. Bacterial growth inhibition was tested by well diffusion method. Inhibition zone was measured using a caliper. Results: The growth inhibition from the largest to the smallest was shown by 75%, 100% and 50% bajakah wood extracts with mean inhibition zone diameters of 5.05 mm, 3.84 mm and 2.02 mm respectively, meanwhile the positive control was 16.14 mm. One Way Anova test showed significant difference in inhibition (p<0.05) in all groups. Post hoc test showed no significant difference in the inhibition of 75% bajakah tampala wood extract with 100% extract. The inhibition of 100% and 75% extracts was significantly greater than 50% extract. The inhibition of NaOCL 2.5% was significantly greater than all groups of test extracts. Conclusion: Bajakah tampala wood extract has inhibitory activity against the growth of Pseudomonas aeruginosa bacteria.

Maple compounds prevent biofilm formation in Listeria monocytogenes via sortase inhibition.

The Pss exopolysaccharide (EPS) enhances the ability of the foodborne pathogen Listeria monocytogenes to colonize and persist on surfaces of fresh fruits and vegetables. Eradicating listeria within EPS-rich biofilms is challenging due to their increased tolerance to disinfectants, desiccation, and other stressors. Recently, we discovered that extracts of maple wood, including maple sap, are a potent source of antibiofilm agents. Maple lignans, such as nortrachelogenin-8'-O-β-D-glucopyranoside and lariciresinol, were found to inhibit the formation of, and promote the dispersion of pre-formed L. monocytogenes EPS biofilms. However, the mechanism remained unknown. Here, we report that these lignans do not affect Pss EPS synthesis or degradation. Instead, they promote EPS detachment, likely by interfering with an unidentified lectin that keeps EPS attached to the cell surfaces. Furthermore, the maple lignans inhibit the activity of L. monocytogenes sortase A (SrtA) in vitro. SrtA is a transpeptidase that covalently anchors surface proteins, including the Pss-specific lectin, to the cell wall peptidoglycan. Consistent with this, deletion of the srtA gene results in Pss EPS detachment from listerial cells. We also identified several additional maple compounds, including epicatechin gallate, isoscopoletin, scopoletin, and abscisic acid, which inhibit L. monocytogenes SrtA activity in vitro and prevent biofilm formation. Molecular modelling indicates that, despite their structural diversity, these compounds preferentially bind to the SrtA active site. Since maple products are abundant and safe for consumption, our finding that they prevent biofilm formation in L. monocytogenes offers a viable source for protecting fresh produce from this foodborne pathogen.

Rapid real-time quantitative colorimetric LAMP methodology for field detection of Verticillium dahliae in crude olive-plant samples

BackgroundVerticilium dahliae is the most important wilt pathogen of olive trees with a broad host range causing devastating diseases currently without any effective chemical control. Traditional detection methodologies are based on symptoms-observation or lab-detection using time consuming culturing or molecular techniques. Therefore, there is an increasing need for portable tools that can detect rapidly V. dahliae in the field.ResultsIn this work, we report the development of a novel method for the rapid, reliable and on-site detection of V. dahliae using a newly designed isothermal LAMP assay and crude extracts of olive wood. For the detection of the fungus, LAMP primers were designed targeting the internal transcribed spacer (ITS) region of the rRNA gene. The above assay was combined with a purpose-built prototype portable device which allowed real time quantitative colorimetric detection of V. dahliae in 35 min. The limit of detection of our assay was found to be 0.8 fg/μl reaction and the specificity 100% as indicated by zero cross-reactivity to common pathogens found in olive trees. Moreover, detection of V. dahliae in purified DNA gave a sensitivity of 100% (Ct < 30) and 80% (Ct > 30) while the detection of the fungus in unpurified crude wood extracts showed a sensitivity of 80% when multisampling was implemented. The superiority of the LAMP methodology regarding robustness and sensitivity was demonstrated when only LAMP was able to detect V. dahliae in crude samples from naturally infected trees with very low infection levels, while nested PCR and SYBR qPCR failed to detect the pathogen in an unpurified form.ConclusionsThis study describes the development of a new real time LAMP assay, targeting the ITS region of the rRNA gene of V. dahliae in olive trees combined with a 3D-printed portable device for field testing using a tablet. The assay is characterized by high sensitivity and specificity as well as ability to operate using directly crude samples such as woody tissue or petioles. The reported methodology is setting the basis for the development of an on-site detection methodology for V. dahliae in olive trees, but also for other plant pathogens.