Wood Decay Research Articles

Impact of Norway spruce pre-degradation stages induced by Gloeophyllum trabeum on fungal and bacterial communities

In forest ecosystems, fungi are the key actors in wood decay. They have the capability to degrade lignified substrates and the woody biomass of coniferous forests, with brown rot fungi being common colonizers. Brown rots are typically involved in the earliest phase of lignocellulose breakdown, which therefore influences colonization by other microorganisms. However, few studies have focused on the impact of introducing decayed wood into forest environments to gauge successional colonization by natural bacterial and fungal communities following partial decay. This study aimed to address this issue by investigating the bacterial and fungal colonization of Norway spruce ( Picea abies ) wood, after intermediate and advanced laboratory-based, pre-decay, by the brown rot fungus Gloeophyllum trabeum . Using Illumina metabarcoding, the in situ colonization of the wood blocks was monitored 70 days after the blocks were placed on the forest floor and covered with litter. We observed significant changes in the bacterial and fungal communities associated with the pre-decayed stage. Further, the wood substrate condition acted as a gatekeeper by reducing richness for both microbial communities and diversity of fungal communities. Our data also suggest that the growth of some fungal and bacterial species was driven by similar environmental conditions.

Why wood should move in rivers

AbstractLarge wood is inherently mobile in naturally functioning river corridors, yet river management commonly introduces wood that is anchored to limit hazards. Wood that is periodically mobilized is important for: replacing stationary large wood that performs diverse physical and ecological functions; contributing to the disturbance regime of the river corridor; diversifying wood decay states; dispersing organisms and propagules; providing refugia during floodplain inundation and in mobile‐bed channels; dissipating flow energy; and supplying wood to downstream environments including lakes, coastlines, the open ocean, and the deep sea. We briefly review what is known about large wood mobility in river corridors and suggest priorities for ongoing research and river management, including: structural designs that can pass mobile wood; enhancing piece diversity of introduced wood that is anchored in place; quantifying wood mobilization and transport characteristics in natural and managed river corridors; and enhancing documentation of the benefits of wood mobility.

Metabolic activities and ultrastructure imaging at late-stage of wood decomposition in interactive brown rot - white rot fungal combinations

Basidiomycota brown rot fungus ( Fomitopsis pinicola ) and two white rot fungi ( Phlebia radiata , Trichaptum abietinum ) were cultivated on thin slices of spruce wood individually and in interspecies combinations. Within 12 months, F. pinicola substantially decomposed spruce wood observed as mass loss, also in three-species combinations. However, white rot fungi through hyphal interactions negatively affected the brown-rot indicative iron reduction capacity of F. pinicola . Decay-signature gene expression in mycelial interaction zones indicated suppression of brown rot mechanism but stimulation of enzymatic white-rot lignin attack by P. radiata . Wood ultrastructure imaging showed white-rot dominance in the fungal combinations, whereas destructive brown-rot was evident with F. pinicola alone. Our results confirm the dynamic pattern of enzyme production in fungal combinations, and transition from brown to white rot decomposition metabolism during the late stage of wood decay after one year of interspecific interactions.

The Brazilian Amazonian rainforest harbors a high diversity of yeasts associated with rotting wood, including many candidates for new yeast species.

This study investigated the diversity of yeast species associated with rotting wood in Brazilian Amazonian rainforests. A total of 569 yeast strains were isolated from rotting wood samples collected in three Amazonian areas (Universidade Federal do Amazonas-Universidade Federal do Amazonas [UFAM], Piquiá, and Carú) in the municipality of Itacoatiara, Amazon state. The samples were cultured in yeast nitrogen base (YNB)-d-xylose, YNB-xylan, and sugarcane bagasse and corncob hemicellulosic hydrolysates (undiluted and diluted 1:2 and 1:5). Sugiyamaella was the most prevalent genus identified in this work, followed by Kazachstania. The most frequently isolated yeast species were Schwanniomyces polymorphus, Scheffersomyces amazonensis, and Wickerhamomyces sp., respectively. The alpha diversity analyses showed that the dryland forest of UFAM was the most diverse area, while the floodplain forest of Carú was the least. Additionally, the difference in diversity between UFAM and Carú was the highest among the comparisons. Thirty candidates for new yeast species were obtained, representing 36% of the species identified and totaling 101 isolates. Among them were species belonging to the clades Spathaspora, Scheffersomyces, and Sugiyamaella, which are recognized as genera with natural xylose-fermenting yeasts that are often studied for biotechnological and ecological purposes. The results of this work showed that rotting wood collected from the Amazonian rainforest is a tremendous source of diverse yeasts, including candidates for new species.

First Report of Trichoderma crassum Causing Leaf Spot on Tomato (Solanum lycopersicum cv. M82) in Ohio.

Trichoderma is a genus of wood-decaying fungi generally found in soil (Druzhinina and Kubicek 2005). Trichoderma crassum was confirmed to be a sister species to T. virens according to the molecular sequencing results (Chaverri et al. 2003). A foliar disease with ~70% incidence on Solanum lycopersicum was observed in a greenhouse at The Ohio State University (40°0'8'' north latitude, 83°1'36'' west longitude), Columbus, United States, in December 2021. On average up to 60% of the leaves per two-month-old tomato plant were infected. Initially, the dark-grey color and irregular spots appeared at the leaf tips. As the disease progressed, the yellow necrotic lesions were observed surrounding the preformed disease spots. Finally, the infected leaves appeared curled and wilted as a whole. The leaf fragments from three tomato plants 40 inches apart were cut from the diseased lesions and surface sterilized with 75% ethanol (30 seconds) and 1% NaOCl (60 seconds), subsequently rinsed with sterilized deionized water three times. Nine pieces of the sterilized leaf tissues were then placed on the PDA plates at 28℃ in the dark and incubated in one incubator for 4 days. The pure cultures of five isolates were acquired and examined with a light microscope. The fungus from all the isolates changed from white to dark green with the radial pattern and profuse sporulation on the PDA. The produced round conidia were observed under a light microscope (Fig S1). The DNA was extracted from two representative isolates which showed the same morphology. The internal transcribed spacer (ITS) region and a conserved fungal rRNA region were amplified using the primers ITS1/ITS4 (5'-TCCGTAGGTGAACCTGCGG-3' and 5'-TCCTCCGCTTATTGATATGC-3') (White et al. 1990) and SR6f/SR7r (5'-TGTTACGACTTTTACTT-3' and 5'-AGTTAAAAAGCTCGTAGTTG-3') (Hirose et al. 2012), respectively. The PCR products were further sequenced by Sanger sequencing (Table S1). Based on the BLAST results through NCBI website, the ITS sequences of the two isolates were 99% (566/572) and 98% (558/572) identical to Trichoderma crassum DAOM 164916 (EU280067). Their SR sequences both showed 99% (290/293; 289/293) identity to the same strain. The phylogenetic tree was also created with the sequences of ITS region by MEGA software (version 11) (Fig S2). Therefore, the fungus was identified as Trichoderma crassum based on its morphological characteristics (green conidia), Sanger sequencing results, and phylogenetic tree. To complete Koch's postulates, the 5-mm-diameter fungal agar discs of 7-day-old pure cultures were used for the inoculation on 18 healthy leaves of six tomato cv. M82 plants with two-month-old. The sterile pure PDA discs of the equal size were used for the mock inoculation as a comparison. Fungal plug method was chosen in this study because it had been widely applied to characterization of the fungal pathogens causing leaf spot disease (Pornsuriya et al. 2020; Yang et al. 2021). Five days later, the same symptom as those that occurred on the previously naturally infected tomato plants were observed on all the inoculated leaves (Fig S3A). However, there were no symptoms on the leaves with the mock inoculation. The fungus re-isolated from the symptomatic leaves showed the consistent morphology (dark-green color with radial sporulation) with the original isolates (Fig S3B). Thus, Trichoderma crassum was verified as the causal agent of the foliar disease on Solanum lycopersicum cv. M82 in our greenhouse. To our knowledge, it is the first report of Trichoderma crassum leading to the leaf spot and wilt on tomato in Ohio. The identification of the causal agent lays the groundwork for the development of necessary disease management techniques. We acknowledge the funding support from CFAES Internal Grants Program 2021009.

Cellulose Nano Crystals (CNC) as Additive for a Bio-Based Waterborne Acrylic Wood Coating: Decay, Artificial Weathering, Physical and Chemical Tests.

Wood coatings prolong the service life of wood-based products, but they are usually of synthetic origin. The aim of the present article is to reduce the fossil-based compounds in a commercial waterborne acrylic coating by CNC addition and to test its performance. The coatings were applied on European beech and Norway spruce wood in order to test durability against Gloeophyllum trabeum (brown wood rot) and Trametes versicolor (white wood rot). Artificial weathering and blue stain, contact angle, physical tests (adhesion, impact and scratch test), chemical (FTIR) and morpho-anatomical analysis (SEM) were carried out. CNC addition increased viscosity, limiting the spreading of the coating into wood pores as visible after SEM observation, which reduced coating adhesion on the substrate. CNC improved fungal resistance as seen by a reduced mass loss and FTIR spectroscopy thanks to crosslinks formation, which reduced water sorption as well. Color change was not significant, and, on the other hand, glossiness was reduced but resulted as more homogeneous than control. CNC addition gave good results also in blue stain protection. CNC improved scratch resistance, but no visible change to impact was registered. CNC has promising results in coatings depending on wood and fungal species and presence of further commercial additives (biocides).

Effect of Various Mentha sp. Extracts on the Growth of Trichoderma viride and Chaetomium globusom on Agar Medium and Pine Wood

This paper presents the effect of water extracts from the leaves of various Mentha spp. on the growth of selected fungi causing the gray decay of wood. The study determined which of the Mentha spp. extracts used had the best effect on inhibiting the development of fungi on various substrates including pine wood. The best results in the complete inhibition of fungi growth on an agar medium were obtained for the M. × piperita ‘Almira’ extract. Biocidal properties were not achieved on wood samples, although it was noticed that at doses of extracts of 600 g/m2 and higher, the growth of fungi in the initial stages of cultivation was clearly inhibited. Chemical substances in the obtained extracts were characterized by gas chromatography. Oxygen monoterpenes were the dominant group of substances, substances belonging to sesquiterpene hydrocarbons, monoterpene hydrocarbons containing oxygen sesquiterpenes, and one substance belonging to non-terpene hydrocarbons were also identified.

Continuous operation of fungal wheel reactor based on solid-state fermentation for the removal of pharmaceutical and personal care products

Wood-rotting fungi and their enzymatic systems represent promising biocatalysts for the removal of pharmaceuticals and personal care products (PPCPs) from wastewater. We designed a fungal wheel reactor (FWR) based on solid-state fermentation (SSF) of Trametes versicolor and a lignocellulosic substrate, which was used as an immobilization carrier for fungal biomass and the sole initial nutrient source for producing fungal oxidative enzymes. Three pharmaceutical and personal care products, acetaminophen, bisphenol A and carbamazepine, were spiked into the synthetic wastewater and the treatment was carried out under non-sterile conditions. Acetaminophen was completely removed from the FWR until laccase was observed. The acetaminophen removal efficiency was retrieved by replacing the fungal wheel with fresh SSF products. Bisphenol A and carbamazepine were removed via enzymatic activity and adsorption. When the fungal wheel was replaced, acetaminophen began to be completely removed, even after laccase depletion. The microbial community analysis indicated that the continuous removal of acetaminophen was mainly due to the high proportion of T. versicolor. The relative abundance of the co-occurring microbial community might be responsible for the divergence in acetaminophen removal between two of fungal wheel-replaced reactors. Overall, FWRs are promising tools for the removal of PPCPs by highly reactive enzymatic mechanisms as well as adsorption on the carrier surface. By replacing SSF and settled microbial communities, FWRs may continuously contribute to bioremediation over a long-term period.

A Laccase Gene Reporting System That Enables Genetic Manipulations in a Brown Rot Wood Decomposer Fungus Gloeophyllum trabeum.

Brown rot fungi are primary decomposers of wood and litter in northern forests. Relative to other microbes, these fungi have evolved distinct mechanisms that rapidly depolymerize and metabolize cellulose and hemicellulose without digesting the more recalcitrant lignin. Its efficient degradative system has therefore attracted considerable attention for the development of sustainable biomass conversion technologies. However, there has been a significant lack of genetic tools in brown rot species by which to manipulate genes for both mechanistic studies and engineering applications. To advance brown rot genetic studies, we provided a gene-reporting system that can facilitate genetic manipulations in a model fungus Gloeophyllum trabeum. We first optimized a transformation procedure in G. trabeum, and then transformed the fungus into a constitutive laccase producer with a well-studied white rot laccases gene (from Trametes versicolor). With this, we built a gene reporting system based on laccase gene's expression and its rapid assay using an 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) indicator dye. The laccase reporter system was validated robust enough to allow us to test the effects of donor DNA's formats, protoplast viability, and gene regulatory elements on transformation efficiencies. Going forward, we anticipate the toolset provided in this work would expedite phenotyping studies and genetic engineering of brown rot species. IMPORTANCE One of the most ubiquitous types of decomposers in nature, brown rot fungi, has lacked robust genetic tools by which to manipulate genes and understand its biology. Brown rot fungi are primary decomposers in northern forests helping recycle the encased carbons in trees back to ecosystem. Relative to other microbes, these fungi employ distinctive mechanisms to disrupt and consume the lignified polysaccharides in wood. Its decay mechanism allows fast, selective carbohydrate catabolization, but without digesting lignin-a barren component that produces least energy trade back for fungal metabolisms. Thus, its efficient degradative system provides a great platform for developing sustainable biotechnologies for biomass conversions. However, progress has been hampered by the lack genetic tools facilitating mechanistic studies and engineering applications. Here, the laccase reporter system provides a genetic toolset for genetic manipulations in brown rot species, which we expect would advance relevant genetic studies for discovering and harnessing the unique fungal degradative mechanisms.

Evaluation of Non-Conventional Yeasts Isolated from Rotten Wood for Hydrolytic Activities and Xylose Fermentation

Hydrolysis of lignocellulose to fermentable sugars and their subsequent conversion to ethanol remain great challenges in the biofuel industry. Rotten wood is first colonized by bacteria and molds that possess strong hydrolases. Yeasts are also an important group of microorganisms that may participate in wood hydrolysis. Decaying wood could provide a rich natural reservoir of yeasts possessing promising hydrolytic activities, including xylanases, cellulases, β-glucosidases, or abilities essential for the fermentation of pentose sugars derived from lignocellulose degradation, especially xylose. Therefore, the aim of this work was to screen yeasts isolated from rotten wood samples for the production of hydrolytic enzymes directed at lignocellulose components and the ability to ferment xylose, L-arabinose, and cellobiose. Methods. Yeast strains were isolated from 22 samples of rotten wood and identified by phenotypic characteristics according to Kurtzman et al. Hydrolytic properties and the ability of the isolated strains to ferment xylose, L-arabinose, and cellobiose were determined using conventional methods. Results. 30 strains of yeasts and yeast-like micromycetes were isolated from 22 samples of rotten wood in the Holosiivskyi Forest, Kyiv. Based on phenotypic properties, most of the isolated yeasts belonged to ascomycetous yeasts and were represented by the following genera: Candida (8 strains), Debaryomyces (5 strains), Kluyveromyces (5 strains), Pichia (5 strains), Scheffersomyces (2 strains), Lachancea, Hanseniaspora, Saccharomyces, and Geotrichum/Galactomyces. A strain of yeast-like non-photosynthetic alga Prototheca sp. was also detected. Most of the isolated microfungi (66.6% isolates) exhibited extracellular β-glucosidase activity, two Candida tropicalis strains possessed weak pectinase and xylanase activity. None of the isolates demonstrated extracellular cellulase activity. Two yeast strains preliminarily identified as Scheffersomyces stipitis were able to ferment xylose at a concentration of 20—100 g/L over a wide temperature range up to 37°C. Acetic acid at 0.25—1% (v/v) concentration resulted in the complete inhibition of xylose fermentation. Ethanol production from xylose up to 6 g/L was observed under the microaerobic fermentation conditions for 24 hr at the substrate concentration 40 g/L, but the subsequent fermentation resulted in decreasing ethanol concentration presumably due to ethanol re-assimilation. None of the isolated strains was capable of fermenting cellobiose or L-arabinose under the microaerobic conditions. Conclusions. This work provides the characterization of yeast microbiota of rotten wood that was represented predominantly by ascomycetous yeasts. The dominant extracellular hydrolytic activity of the isolates was β-glucosidase. This is the first report on the isolation of xylose-fermenting yeasts Scheffersomyces stipitis in Ukraine, which comprised 7% of all the microfungi isolated from rotten wood.

Moroccan Endemic Artemisia herba-alba Essential Oil: GC-MS Analysis and Antibacterial and Antifungal Investigation

In Morocco, the endemic Artemisia herba-alba is well known by its traditional uses and health benefits. The search for natural, safe, and effective antibacterial and antifungal agents from plants is in high demand due to microbial and fungal resistance to conventional synthetic antibiotics and antifungal drugs. In this study, the A. herba-alba was collected from the region of Fez-Boulemane during the periods of March, June, and September. Essential oils (EOs) were extracted from the aerial part of the plant by the hydrodistillation method. The chemical constituents were determined using GC-MS as analytical tools. The antimicrobial activities of different oils were tested using the macrodilution method. The results showed the difference in the yields between the three EOs (0.49, 1.74, 1.30% (mL/100 g)), respectively, as well as in their corresponding chemical compositions. The main constituents revealed by GC-MS are higher contents of oxygenated monoterpenes (84.7, 84.4, 81%), such as cis chrysanthenyl acetate (30, 26.7, 27.6%), β-thujone (23.2, 12.9, 15.4%), camphor (9.76, 14.3, 15.8%), chrysanthenone (2.4, 1, 14%), 1,8-cineole (1.5, 11.7, 11.8%), trans β-dihydroterpineol (7.8, 7.2, 6.9%), α-thujone (4.8, 3, 5.4%), and sesquiterpenic davanone (3.9, 1.5, 1.4%), respectively. The three EOs biological activities’ results showed significant antimicrobial effects against four bacteria tested (E. coli, B. subtilis, S. aureus, M. luteus), with the MIC values ranging from 0.1 to 0.03% (v/v), as well as interesting antifungal effects on both wood rot fungi against four fungi examined (G. trabeum, P. placenta, C. puteana, C. versicolor) and molds against three microorganisms tested (A. niger, P. digitatum, P. expansum), with MIC values ranging from 0.2 to 0.03% (v/v) and 0.4 to 0.03% (v/v), respectively. The June and September EO samples showed more potent activities than those collected during March. Our research findings showed quantitative variability in both EO contents and chemical compositions, which could be due to the phenological stages, climatic conditions of growth, and harvesting periods. The potent results of the antimicrobial/antifungal activities were provided by the EOs of June and September and might be correlated to the contribution and synergism effect of all oxygenated monoterpenes. These results support the possible application of A. herba-alba EOs as natural and safe antibacterial agents, and an effective alternative to synthetic drugs, enabling the prevention and treatment of certain pathogenic infections in food and health, and the preservation of wood alteration against fungi.

Fungal trunk diseases of fruit trees in Europe: pathogens, spread and future directions

Production from crops of pome, stone fruit, nut, berry fruit, citrus, grapevine, and olive is increasingly threatened by fungal trunk diseases (FTD). These diseases and the consequent production losses are major problems. Many fungi (including Botryosphaeriaceae, Calosphaeriaceae, Diaporthaceae, Diatrypaceae, Nectriaceae, Phaeomoniellaceae, Pleosporaceae, Togniniaceae, Valsaceae) infect host wood, mainly through wounds and subsequent colonization of woody tissues, causing symptoms such as cankers, gummosis, wood rotting, blight and dieback. Propagative plant material, seedlings and fruit play a significant role in pathogen spread. Several abiotic factors (e.g. shifts in cultural practices and climate change) are involved in the disease development. This paper reviews recent literature on FTD of fruit crops, particularly focusing on the European status of pathogen occurrence. Case studies are described related to diseases of apple, citrus, grapevine, berry, nut and stone fruit, and olive trees. Aspects related to epidemiology and the increase in disease incidence along with the future perspectives on the FTD research are also discussed.

Lignin-Modifying Enzymes in Scedosporium Species.

Scedosporium species are usually soil saprophytes but some members of the genus such as S. apiospermum and S. aurantiacum have been regularly reported as causing human respiratory infections, particularly in patients with cystic fibrosis (CF). Because of their low sensitivity to almost all available antifungal drugs, a better understanding of the pathogenic mechanisms of these fungi is mandatory. Likewise, identification of the origin of the contamination of patients with CF may be helpful to propose prophylactic measures. In this aim, environmental studies were conducted demonstrating that Scedosporium species are abundant in human-made environments and associated with nutrient-rich substrates. Although their natural habitat remains unknown, there is accumulated evidence to consider them as wood-decaying fungi. This study aimed to demonstrate the ability of these fungi to utilize lignocellulose compounds, especially lignin, as a carbon source. First, the lignolytic properties of Scedosporium species were confirmed by cultural methods, and biochemical assays suggested the involvement of peroxidases and oxidases as lignin-modifying enzymes. Scedosporium genomes were then screened using tBLASTn searches. Fifteen candidate genes were identified, including four peroxidase and seven oxidase genes, and some of them were shown, by real-time PCR experiments, to be overexpressed in lignin-containing medium, thus confirming their involvement in lignin degradation.

First fossil eleusinin rove beetle (Coleoptera: Staphylinidae: Osoriinae: Eleusinini) from mid-Cretaceous Kachin amber of northern Myanmar and its evolutionary implications

Eleusinini is the smallest osoriine tribe, consisting of 221 species in three genera. No fossil Eleusinini has been found until now. Here, I describe the first extinct representative of eleusinin rove beetles, Eleusis sulcata n. sp., based on a well-preserved adult specimen from the mid-Cretaceous Kachin amber in northern Myanmar. This new species is tentatively placed in the extant genus EleusisLaporte, 1835, and it is easily distinguished from its congeners by the longitudinal sulcus on the dorsal surface of the head and complete lack of supraocular grooves. The discovery of E. sulcata n. sp. provides further evidence that Eleusinini is a relatively ancient group, originating no later than the Albian–Cenomanian boundary. Furthermore, the markedly flattened body of this new species suggests that the Eleusinini had adapted to a subcortical lifestyle under bark or in rotten wood by the mid-Cretaceous.

Earthworms are not just “earth” worms: Multiple drivers to large diversity in deadwood

Earthworms are ecosystem engineers associated with important soil functions. Despite the large amount of literature on earthworm ecology, relatively few studies have examined earthworms in deadwood or quantified their importance in this habitat. We investigated earthworm communities in decaying deadwood and disentangled how their community dynamics are influenced by variation in tree species, wood decomposition stage, and forest environment. Decaying logs (of standardised size) of ten common, temperate tree species were laid out to decay on the soil surface for four years. The experiment was carried out in the “tree cemetery” experiment LOGLIFE, in two contrasting temperate forests in the central Netherlands. The decaying logs yielded surprisingly rich earthworm populations, with on average 19 individuals per meter of log and in total belonging to 12 different species. Our findings highlighted that earthworm communities in deadwood in terms of composition and abundance were influenced by tree species, wood decomposition stage and forest type with different soil properties, as well as their interactions. After one and two years of decay, earthworm abundance was higher in the logs of Picea abies and relatively fast decomposing Populus spp. than in other trees, while this pattern changed after four years with higher earthworm abundance observed in the other tree species. Overall, Populus spp. supported the highest earthworm abundance, followed by Picea abies and Quercus robur. The earthworm community composition in the logs in the two forest sites had broadly similar dynamic trends of first becoming very dissimilar between one and two years, then relatively more similar from two to four years of decay, although the community composition differed between sites. The interacting influences of tree species, wood decay stage and forest environment on earthworm communities strongly suggest that diversity in deadwood resources contributes to earthworm diversity in forests. Thus, by mixing different tree species and logging gradually through the years, forest managers could enhance the diversity of this abundant and understudied component of deadwood invertebrate diversity. Further research should study the feedback loop between earthworm communities and decomposition of deadwood.

Effectiveness of three Gabonese tree resin fractions combined with tebuconazole as wood preservative formulations

Wood treatments based on oleoresins from Aucoumea Klaineana, Canarium schweinfurthii and Dacryodes edulis have shown interesting anti-termites properties but poor resistance to fungi and leaching. Oleoresins were combined with tebuconazole to produce both antifungal and anti-termite wood preservatives formulations with an expected better fixation of chemicals mixtures in the wood. Beech and Scots pine wood block samples were impregnated by these different formulations and exposed to white and brown rot, respectively, as well as to European termite (Reticulitermes flavipes). The use of aqueous formulations of each oleoresin of combined to tebuconazole improved the wood resistance against termites and mainly brown rot. In addition, the best formulation was found by using the raw resin from Aucoumea Klaineana. These results are mainly due to the antifungal activity of tebuconazole. In addition, the presence of antioxidant chemicals in the wood resins inhibits the wood decay fungi growth and acts as enzyme and metabolism inhibitors disturbing the treated wood digestion process by termites. For the future, the promising protection obtained should be formulated to be resistant to leaching.

Interference of wood decay, growth, and infection ofGanoderma boninenseby ligninolytic fungi from herbaceous plants

Basal stem rot (BSR) disease caused byGanoderma boninenseis the most destructive disease in oil palm plantations. Herbaceous plants such as arrowroot, cocoyam, and canna were reported to interfere with theGanodermadisease of oil palm under mixed planting on non-sterilized soil. This study aimed to determine the role of the ligninolytic fungi isolated from herbaceous plants on wood decay, mycelial growth, and infection ofGanoderma boninense. A total of 24 ligninolytic fungal isolates were isolated from arrowroot, cocoyam, and canna plant and grouped into 6 types of wood decay interaction, namely (1) neutral, (2) negative interference to the herbaceous isolates, (3) negative interference to both fungi, (4) negative interference toGanoderma, (5) negative interference to the herbaceous isolates and positive forGanoderma, and (6) positive interference in both fungi. Ligninolytic fungi from cocoyam and canna plants were able to negatively interfere with theGanodermawood decay, inhibit the colony, and reduce the initial root infection of oil palm. Keywords: antagonistic in vitro,Ganoderma boninense, ligninolytic fungi, root necrosis, wood decay.

Co-culture Wood Block Decay Test with Bacteria and Wood Rotting Fungi to Analyse Synergism/Antagonism during Wood Degradation.

Mixed communities of fungi and bacteria have been shown to be more efficient in degrading wood than fungi alone. Some standardised protocols for quantification of the wood decay ability of fungi have been developed (e.g., DIN V ENV 12038:2002 as the legal standard to test for the resistance of wood against wood-destroying basidiomycetes in Germany). Here, we describe a step-by-step protocol developed from the official standard DIN V ENV12038 to test combinations of bacteria and fungi for their combined wood degradation ability. Equally sized wood blocks are inoculated with wood decay fungi and bacterial strains. Axenic controls allow the analysis of varying degradation rates via comparison of the wood dry weights at the end of the experiments. This protocol provides new opportunities in exploration of inter- and intra-kingdom interactions in the wood-related environment and forms the basis for microcosm experiments. Key features • Quantification of wood decay ability of mixed cultures. • Allows testing if fungi are more efficient in degrading wood when bacteria are present.

How climate change will change the potential distribution of two wood-decaying fungi?

How climate change will change the potential distribution of two wood-decaying fungi?

Antivirusna svojstva lignikolnih gljiva Srbije

Fungi have multiple roles in nature. However, from his point of view, man most often views them as useful or harmful (depending on his primary activity). Thus, wood decaying fungi are most often seen as parasitic and saprophytic organisms, economically harmful, overlooking their useful functions. One of their useful functions is a possibility of their use in the treatment of various diseases. The results of the research of antiviral properties of lignicolous fungi are presented in this paper. Samples for isolation and identification of fungi are collected on several sites in Serbia. It has been determined that the fungi that cause wood decay have various antiviral properties, most often against viruses such as influenza, hepatitis, herpes, SARS-CoV-2 and HIV.