Nutritional status, yield and fruit quality of Wonderful pomegranate trees grown in sandy calcareous soil in response to spraying Royal jelly at 0.0125, 0.0250 and 0.050% and frequencies, once (at the end May) twice (at the end of May and again at mid-June) and three applications (at the end May, mid-June and the end of August) were investigated during 2017 and 2018 seasons. spraying Royal jelly was very effective in improving the leaf area, leaf chlorophylls contents and total carotenoids, N, P, and K, yield and fruit quality rather than non- application. The gradual Increasing in Royal jelly concentration and frequencies was remarkably enhancing yield and fruit quality. However, non-significant differences were observed between the two heights concentrations. The best results with regard to vegetative growth, yield and fruit quality of Wonderful pomegranate trees grown under sandy calcareous soil were obtained when the trees received three sprays of Royal jelly at 0.05%. However non-significant differences were obtained between the two highest concentrations of Royal Jelly namely; 0.0250 and 0.050%.